目的研究益气活血复方对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)Toll样受体4(TLR4)及其下游信号转导通路元件髓样分化因子88(MyD88)、肿瘤坏死因子受体相关因子-6(TRAF-6)、Toll样受体相关分子(TRAM)、Toll样受体相关的干扰素活化子...目的研究益气活血复方对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)Toll样受体4(TLR4)及其下游信号转导通路元件髓样分化因子88(MyD88)、肿瘤坏死因子受体相关因子-6(TRAF-6)、Toll样受体相关分子(TRAM)、Toll样受体相关的干扰素活化子(TRIF)表达的影响,探讨益气活血复方含药血清防治动脉粥样硬化(AS)的机制。方法选择新西兰大耳白兔20只,随机分为4组,即正常组、中药高浓度组、中药中浓度组、中药低浓度组,每组5只。以上各组白兔分别以生理盐水和高、中、低浓度益气活血复方连续灌胃7d。末次灌胃给药2h后,心脏采血,离心后分离血清。体外培养人脐静脉内皮细胞,用LPS刺激后,分别加入高、中、低浓度益气活血复方含药血清干预24h,收集细胞,用Real ti me PCR方法测定TLR4、MyD88、TRAF-6、TRAM及TRIF mRNA的表达。结果用LPS刺激人脐静脉内皮细胞后,引起TLR4、MyD88、TRAF-6、TRAM及TRIF mRNA的高表达(与空白对照组比较,P<0.01),用益气活血复方含药血清干预以后显著抑制TLR4、MyD88及TRAF-6 mRNA的高表达(与模型组比较P<0.05或P<0.01),对TRAM及TRIF作用不明显。结论益气活血复方可阻断TLR4高表达,同时阻断TLR4胞内信号转导的MyD88依赖性途径,而对MyD88非依赖性途径作用不明显,因此益气活血复方主要是通过阻断MyD88依赖性途径来发挥其抗动脉粥样硬化的作用。展开更多
This review summarizes the general developments of the operational mesoscale model system based on the Global/Regional Assimilation and Prediction System-Tropical Monsoon Model (GRAPES-TMM) at the Guangzhou Regional M...This review summarizes the general developments of the operational mesoscale model system based on the Global/Regional Assimilation and Prediction System-Tropical Monsoon Model (GRAPES-TMM) at the Guangzhou Regional Meteorological Center. GRAPES-TMM consists of the Tropical Regional Atmospheric Model System for the South China Sea (TRAMS, a typhoon model with a horizontal resolution of 9 km), the Mesoscale Atmospheric Regional Model System (MARS, 3km) and the fine-scale Rapid Update Cycling (RUC, 1km) forecasting system. The main advances of model dynamical core and physical processes are summarized, including the development of the 3D reference atmosphere scheme, the coupling scheme between dynamics and model physics, the calculation of nonlinear terms by fractional steps, the gravity wave drag scheme induced by sub-grid orography and a simplified model for landsurface scheme. The progress of model applications is reviewed and evaluated. The results show that the updated 9-3-1forecasting system provides an overall improved performance on the weather forecasting in south China, especially for typhoon-genesis and typhoon-track forecasting as well as short-range weather forecasting. Capabilities and limitations as well as the future development of the forecasting system are also discussed.展开更多
Recent innovations in transport technology are now providing mobility that is cheaper, autonomous, electric, and with improved ride quality. While much of the world’s attention has been on how this can be applied to ...Recent innovations in transport technology are now providing mobility that is cheaper, autonomous, electric, and with improved ride quality. While much of the world’s attention has been on how this can be applied to cars, there have been rapid adoption of these and other technologies in High Speed Rail and Metro Rail systems that run between and across cities. This paper shows how such innovations have now been applied to create the next generation of urban transit system called a Trackless Tram. Trackless Trams are effectively the same as traditional light rail except they run on rubber tyres avoiding disruption from construction for Light Rail, but they retain the electric propulsion (with batteries) and have high ride quality due to rail-type bogies, stabilization technologies and precision tracking from the autonomous optical guidance systems—with infrastructure costs reduced to as low as one tenth of a Light Rail system. As with Light Rail, a Trackless Tram System provides a rapid transit option that can harness the fixed route assurance necessary to unlock new land value appreciation that can be leveraged to contribute to construction and running costs whilst creating urban regeneration. The paper considers the niche for Trackless Trams in cities along with its potential for city shaping through the creation of urban re-development along corridors. The paper suggests that the adoption of Trackless Tram Systems is likely to grow rapidly as a genuine alternative to car and bus systems, supplementing and extending the niche occupied by Light Rail Transit (LRT). This appears to be feasible in any medium-sized or larger city, especially in emerging and developing economies, and case studies are outlined for Perth and Thimpu to illustrate its potential.展开更多
文摘目的研究益气活血复方对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)Toll样受体4(TLR4)及其下游信号转导通路元件髓样分化因子88(MyD88)、肿瘤坏死因子受体相关因子-6(TRAF-6)、Toll样受体相关分子(TRAM)、Toll样受体相关的干扰素活化子(TRIF)表达的影响,探讨益气活血复方含药血清防治动脉粥样硬化(AS)的机制。方法选择新西兰大耳白兔20只,随机分为4组,即正常组、中药高浓度组、中药中浓度组、中药低浓度组,每组5只。以上各组白兔分别以生理盐水和高、中、低浓度益气活血复方连续灌胃7d。末次灌胃给药2h后,心脏采血,离心后分离血清。体外培养人脐静脉内皮细胞,用LPS刺激后,分别加入高、中、低浓度益气活血复方含药血清干预24h,收集细胞,用Real ti me PCR方法测定TLR4、MyD88、TRAF-6、TRAM及TRIF mRNA的表达。结果用LPS刺激人脐静脉内皮细胞后,引起TLR4、MyD88、TRAF-6、TRAM及TRIF mRNA的高表达(与空白对照组比较,P<0.01),用益气活血复方含药血清干预以后显著抑制TLR4、MyD88及TRAF-6 mRNA的高表达(与模型组比较P<0.05或P<0.01),对TRAM及TRIF作用不明显。结论益气活血复方可阻断TLR4高表达,同时阻断TLR4胞内信号转导的MyD88依赖性途径,而对MyD88非依赖性途径作用不明显,因此益气活血复方主要是通过阻断MyD88依赖性途径来发挥其抗动脉粥样硬化的作用。
基金National Key R&D Program of China(2018YFC1506901)National Natural Science Foundation of China (41505084)Program of Science and Technology Department of Guangdong Province (201804020038)。
文摘This review summarizes the general developments of the operational mesoscale model system based on the Global/Regional Assimilation and Prediction System-Tropical Monsoon Model (GRAPES-TMM) at the Guangzhou Regional Meteorological Center. GRAPES-TMM consists of the Tropical Regional Atmospheric Model System for the South China Sea (TRAMS, a typhoon model with a horizontal resolution of 9 km), the Mesoscale Atmospheric Regional Model System (MARS, 3km) and the fine-scale Rapid Update Cycling (RUC, 1km) forecasting system. The main advances of model dynamical core and physical processes are summarized, including the development of the 3D reference atmosphere scheme, the coupling scheme between dynamics and model physics, the calculation of nonlinear terms by fractional steps, the gravity wave drag scheme induced by sub-grid orography and a simplified model for landsurface scheme. The progress of model applications is reviewed and evaluated. The results show that the updated 9-3-1forecasting system provides an overall improved performance on the weather forecasting in south China, especially for typhoon-genesis and typhoon-track forecasting as well as short-range weather forecasting. Capabilities and limitations as well as the future development of the forecasting system are also discussed.
文摘Recent innovations in transport technology are now providing mobility that is cheaper, autonomous, electric, and with improved ride quality. While much of the world’s attention has been on how this can be applied to cars, there have been rapid adoption of these and other technologies in High Speed Rail and Metro Rail systems that run between and across cities. This paper shows how such innovations have now been applied to create the next generation of urban transit system called a Trackless Tram. Trackless Trams are effectively the same as traditional light rail except they run on rubber tyres avoiding disruption from construction for Light Rail, but they retain the electric propulsion (with batteries) and have high ride quality due to rail-type bogies, stabilization technologies and precision tracking from the autonomous optical guidance systems—with infrastructure costs reduced to as low as one tenth of a Light Rail system. As with Light Rail, a Trackless Tram System provides a rapid transit option that can harness the fixed route assurance necessary to unlock new land value appreciation that can be leveraged to contribute to construction and running costs whilst creating urban regeneration. The paper considers the niche for Trackless Trams in cities along with its potential for city shaping through the creation of urban re-development along corridors. The paper suggests that the adoption of Trackless Tram Systems is likely to grow rapidly as a genuine alternative to car and bus systems, supplementing and extending the niche occupied by Light Rail Transit (LRT). This appears to be feasible in any medium-sized or larger city, especially in emerging and developing economies, and case studies are outlined for Perth and Thimpu to illustrate its potential.
