To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic a...To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.展开更多
目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重...目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重度新生儿高胆红素血症的患儿为研究对象,按照其接受干预方式分为研究组(45例)和对照组(40例)。研究组联用白蛋白与光疗治疗,对照组接受单纯光疗治疗。比较两组患儿临床干预效果、干预过程中TBA、TSB等实验室指标的变化情况,并统计两组患儿治疗过程中并发症的发生情况。结果研究组患者治疗总有效率高于对照组,差异有统计学意义(P<0.05)。干预12~72 h,研究组患儿的TBA和TSB水平均低于对照组,差异有统计学意义(P<0.05)。干预前,两组患儿超敏C反应蛋白(HS-CRP)、游离脂肪酸(FFA)水平比较,差异无统计学意义(P>0.05);干预后,研究组患儿HS-CRP、FFA水平均低于对照组,差异有统计学意义(P<0.05)。两组患儿不良反应发生率比较,差异无统计学意义(P>0.05)。结论白蛋白联合光疗对重度新生儿高胆红素血症患儿具有较好的干预效果,能够显著降低新生儿TBA及TSB水平,治疗安全性较好,值得临床应用。展开更多
目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜...目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜形成的影响;提取细菌总RNA,逆转录后进行实时荧光定量PCR检测3种培养基对表皮葡萄球菌粘附基因表达量的影响。结果与LB(0.149±0.047)和M-H(0.323±0.003)培养基相比,TSB(2.954±0.287)培养基能显著促进表皮葡萄球菌生物膜的形成(TSB vs LB,t=16.706,P<0.01;TSB vs M-H,t=15.877,P<0.01);与LB培养基相比,TSB培养基可显著促进表皮葡萄球菌ica A基因的表达(t=9.667,P<0.01)并抑制icaR基因的表达(t=13.283,P<0.01)。结论与LB和M-H肉汤培养基相比,TSB培养基能显著促进表皮葡萄球菌生物膜的形成和粘附基因ica A的表达。展开更多
文摘To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
文摘目的探究综合运用白蛋白和光疗对治疗重度新生儿高胆红素血症的效果及对患儿总胆汁酸(total bile acid,TBA)、总胆红素(total bilirubin,TSB)水平的影响。方法选择2020年1月—2022年12月于淮安市淮阴区妇幼保健院接受临床干预的85例重度新生儿高胆红素血症的患儿为研究对象,按照其接受干预方式分为研究组(45例)和对照组(40例)。研究组联用白蛋白与光疗治疗,对照组接受单纯光疗治疗。比较两组患儿临床干预效果、干预过程中TBA、TSB等实验室指标的变化情况,并统计两组患儿治疗过程中并发症的发生情况。结果研究组患者治疗总有效率高于对照组,差异有统计学意义(P<0.05)。干预12~72 h,研究组患儿的TBA和TSB水平均低于对照组,差异有统计学意义(P<0.05)。干预前,两组患儿超敏C反应蛋白(HS-CRP)、游离脂肪酸(FFA)水平比较,差异无统计学意义(P>0.05);干预后,研究组患儿HS-CRP、FFA水平均低于对照组,差异有统计学意义(P<0.05)。两组患儿不良反应发生率比较,差异无统计学意义(P>0.05)。结论白蛋白联合光疗对重度新生儿高胆红素血症患儿具有较好的干预效果,能够显著降低新生儿TBA及TSB水平,治疗安全性较好,值得临床应用。
文摘目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜形成的影响;提取细菌总RNA,逆转录后进行实时荧光定量PCR检测3种培养基对表皮葡萄球菌粘附基因表达量的影响。结果与LB(0.149±0.047)和M-H(0.323±0.003)培养基相比,TSB(2.954±0.287)培养基能显著促进表皮葡萄球菌生物膜的形成(TSB vs LB,t=16.706,P<0.01;TSB vs M-H,t=15.877,P<0.01);与LB培养基相比,TSB培养基可显著促进表皮葡萄球菌ica A基因的表达(t=9.667,P<0.01)并抑制icaR基因的表达(t=13.283,P<0.01)。结论与LB和M-H肉汤培养基相比,TSB培养基能显著促进表皮葡萄球菌生物膜的形成和粘附基因ica A的表达。
