Hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality.Immunotherapy has emerged in clinical treatment,owing to the limitation and severe side effects of chemotherapy.In the immune sy...Hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality.Immunotherapy has emerged in clinical treatment,owing to the limitation and severe side effects of chemotherapy.In the immune system,natural killer（NK）cells are important effectors required to eliminate malignant tumor cells without the limitation of major histocompatibility complex（MHC）molecule issues.Hence,treatment which could stimulate NK cells is of great interest.Here,we investigated the efficacy of the combined therapy of TT-1（a mutant of melittin）and interferon-α（IFN-α）on NK cells and human liver cancer HepG-2/Huh7 cells in vitro and in vivo,as well as the mechanism involved.The combination therapy significantly inhibited the growth of HepG-2/Huh7 cells in vivo,but this effect was impaired after depleting NK cells.TT-1 not only up-regulated MHC class I-related chain molecules A（MICA）expression,but also prevented the secretion of soluble MICA（sM ICA）.Both the mR NA and protein of a disintegrin and metallopeptidase 10（ADAM 10）in HepG-2/Huh7 cells were decreased after TT-1 treatment.The combined therapy of TT-1 and IFN-αcould suppress the growth of HepG-2/Huh7 xenografted tumor effectively via promoting the interaction of NK group 2,member D（NKG2D）and MICA,indicating that TT-1＋IFN-αwould be a potential approach in treating liver cancer.展开更多
针对深空测控系统高精度测量对于信道附加相噪的要求,采用直接数字频率合成(DDS)正交调制方法设计频率综合器。通过巧妙的试验和外推方法,择优选取电压型鉴相器,在锁相环相噪模型的基础上,全面分析各部分相噪的贡献,综合设计环路带宽,...针对深空测控系统高精度测量对于信道附加相噪的要求,采用直接数字频率合成(DDS)正交调制方法设计频率综合器。通过巧妙的试验和外推方法,择优选取电压型鉴相器,在锁相环相噪模型的基础上,全面分析各部分相噪的贡献,综合设计环路带宽,有效控制附加相噪,实现低相噪频综器最理想的目标,即环路带内的相噪完全由参考决定,带外的相噪由压控振荡器(VCO)决定,并采用两源互比的方法完成1 Hz极低相位噪声的测试,测试结果为-73 d Bc/Hz,与设计结果完全一致。该方法对于测控站极低相噪的设计具有一定参考价值。展开更多
＂Tian Tuo 1＂ （TT-1） nano-satellite is the first single-board nano-satellite that was suc- cessfully launched in China. The main objective of TT-1 is technology demonstration and scientific measurements. The satell...＂Tian Tuo 1＂ （TT-1） nano-satellite is the first single-board nano-satellite that was suc- cessfully launched in China. The main objective of TT-1 is technology demonstration and scientific measurements. The satellite carries out the significant exploration of single-board architecture fea- sibility validation, and it is tailored to the low-cost philosophy by adopting numerous commercial- off-the-shelf （COTS） components. The satellite is featured with three-axis stabilization control capability. A pitch bias momentum wheel and three magnetic coils are adopted as control actuators. The sun sensors, magnetometers and a three-axis gyro are employed as the measurement sensors. The quaternion estimator （QUEST） and unscented Kalman filter （UKF） method are adopted for the nano-satellite attitude determination. On-orbit data received by ground station is conducted to analysis the performance of attitude determination and control system （ADCS）. The results show that the design of ADCS for TT-1 is suitable, robust and feasible.展开更多
基金supported by the Outstanding Young Talent Foundation Project of Science and Technology Department in Jilin Province(No.20170520018JH),China
文摘Hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality.Immunotherapy has emerged in clinical treatment,owing to the limitation and severe side effects of chemotherapy.In the immune system,natural killer（NK）cells are important effectors required to eliminate malignant tumor cells without the limitation of major histocompatibility complex（MHC）molecule issues.Hence,treatment which could stimulate NK cells is of great interest.Here,we investigated the efficacy of the combined therapy of TT-1（a mutant of melittin）and interferon-α（IFN-α）on NK cells and human liver cancer HepG-2/Huh7 cells in vitro and in vivo,as well as the mechanism involved.The combination therapy significantly inhibited the growth of HepG-2/Huh7 cells in vivo,but this effect was impaired after depleting NK cells.TT-1 not only up-regulated MHC class I-related chain molecules A（MICA）expression,but also prevented the secretion of soluble MICA（sM ICA）.Both the mR NA and protein of a disintegrin and metallopeptidase 10（ADAM 10）in HepG-2/Huh7 cells were decreased after TT-1 treatment.The combined therapy of TT-1 and IFN-αcould suppress the growth of HepG-2/Huh7 xenografted tumor effectively via promoting the interaction of NK group 2,member D（NKG2D）and MICA,indicating that TT-1＋IFN-αwould be a potential approach in treating liver cancer.
文摘针对深空测控系统高精度测量对于信道附加相噪的要求,采用直接数字频率合成(DDS)正交调制方法设计频率综合器。通过巧妙的试验和外推方法,择优选取电压型鉴相器,在锁相环相噪模型的基础上,全面分析各部分相噪的贡献,综合设计环路带宽,有效控制附加相噪,实现低相噪频综器最理想的目标,即环路带内的相噪完全由参考决定,带外的相噪由压控振荡器(VCO)决定,并采用两源互比的方法完成1 Hz极低相位噪声的测试,测试结果为-73 d Bc/Hz,与设计结果完全一致。该方法对于测控站极低相噪的设计具有一定参考价值。
文摘＂Tian Tuo 1＂ （TT-1） nano-satellite is the first single-board nano-satellite that was suc- cessfully launched in China. The main objective of TT-1 is technology demonstration and scientific measurements. The satellite carries out the significant exploration of single-board architecture fea- sibility validation, and it is tailored to the low-cost philosophy by adopting numerous commercial- off-the-shelf （COTS） components. The satellite is featured with three-axis stabilization control capability. A pitch bias momentum wheel and three magnetic coils are adopted as control actuators. The sun sensors, magnetometers and a three-axis gyro are employed as the measurement sensors. The quaternion estimator （QUEST） and unscented Kalman filter （UKF） method are adopted for the nano-satellite attitude determination. On-orbit data received by ground station is conducted to analysis the performance of attitude determination and control system （ADCS）. The results show that the design of ADCS for TT-1 is suitable, robust and feasible.
