Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye ...Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).展开更多
In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of...In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of systems with a center in two zones and without equilibrium points in the other zone (or with a center in one zone and without equilibrium points in the other zones). In addition, we also give examples to illustrate that it can reach the maximal number.展开更多
The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened ...The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.展开更多
Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,J...Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.展开更多
BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untran...BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.展开更多
The 10kV distribution network is an essential component of the power system,and its stable operation is crucial for ensuring reliable power supply.However,various factors can lead to faults in the distribution network...The 10kV distribution network is an essential component of the power system,and its stable operation is crucial for ensuring reliable power supply.However,various factors can lead to faults in the distribution network.In order to enhance the safety and reliability of power distribution,this paper focuses on the analysis of faults in the 10kV distribution network caused by natural factors,operational factors,human factors,and equipment factors.It elucidates the various hazards resulting from distribution network faults and proposes corresponding preventive measures for different types of faults in the 10kV distribution network.The aim is to mitigate or reduce the impact of distribution network faults,ensuring the safe and stable operation of the distribution system.展开更多
In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation ...In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.展开更多
Due to the NP-hardness of the two-sided assembly line balancing (TALB) problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In ...Due to the NP-hardness of the two-sided assembly line balancing (TALB) problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In this paper, an effective hybrid algorithm is proposed to address the TALB problem with multiple constraints (TALB-MC). Considering the discrete attribute of TALB-MC and the continuous attribute of the standard teaching-learning-based optimization (TLBO) algorithm, the random-keys method is hired in task permutation representation, for the purpose of bridging the gap between them. Subsequently, a special mechanism for handling multiple constraints is developed. In the mechanism, the directions constraint of each task is ensured by the direction check and adjustment. The zoning constraints and the synchronism constraints are satisfied by teasing out the hidden correlations among constraints. The positional constraint is allowed to be violated to some extent in decoding and punished in cost fimction. Finally, with the TLBO seeking for the global optimum, the variable neighborhood search (VNS) is further hybridized to extend the local search space. The experimental results show that the proposed hybrid algorithm outperforms the late acceptance hill-climbing algorithm (LAHC) for TALB-MC in most cases, especially for large-size problems with multiple constraints, and demonstrates well balance between the exploration and the exploitation. This research proposes an effective and efficient algorithm for solving TALB-MC problem by hybridizing the TLBO and VNS.展开更多
In this paper, the motions are studied of a multi-body which is composed of two plates hinged together and moored by eight mooring lines in regular waves. The experimental results are compared with computational resul...In this paper, the motions are studied of a multi-body which is composed of two plates hinged together and moored by eight mooring lines in regular waves. The experimental results are compared with computational results. The linear potential theory and the perturbation method are combined to study this complicated system. The former is used to calculate the wave forces acting on the plates and the motion responses of them, while the latter is used to describe the dynamic character of the eight mooring lines coupled with the two hinged plates. Some response results of each plate are presented and comparisons between calculated results and experimental data are given. All the calculations are confined to regular beam waves.展开更多
Electrical pollution is a worldwide concern,because it is potentially harmful to human health.Trees not only play a significant role in moderating the climate,but also can be used as shields against electrical polluti...Electrical pollution is a worldwide concern,because it is potentially harmful to human health.Trees not only play a significant role in moderating the climate,but also can be used as shields against electrical pollution.Shielding effects on the electric field strength under transmission lines by two tree species,Populus alba and Larix gmelinii,were examined in this study.The electrical resistivity at different heights of trees was measured using a PiCUS sonic tomograph,which can image the electrical impedance for trees.The electric field strength around the trees was measured with an elf field strength measurement system,HI-3604,and combined with tree resistivity to develop a model for calculating the electric field intensity around trees using the finite element method.In addition,the feasibility of the finite element method was confirmed by comparing the calculated results and experimental data.The results showed that the trees did reduce the electric field strength.The electric field intensity was reduced by 95.6%,and P.alba was better than L.gmelinii at shielding.展开更多
Using the C-mapping topological theory, we study the topological structure of vortex lines in a two-dimensional generalized Gross Pitaevskii theory in (3+l)-dimensional space-time. We obtain the reduced dynamic equ...Using the C-mapping topological theory, we study the topological structure of vortex lines in a two-dimensional generalized Gross Pitaevskii theory in (3+l)-dimensional space-time. We obtain the reduced dynamic equation in the framework of the two-dimensional Gross-Pitaevskii theory, from which a conserved dynamic quantity is derived on the stable vortex lines. Such equations can also be used to discuss Bose-Einstein condensates in heterogeneous and highly nonlinear systems. We obtain an exact dynamic equation with a topological term, which is ignored in traditional hydrodynamic equations. The explicit expression of vorticity as a function of the order parameter is derived, where the function indicates that the vortices can only be generated from the zero points of Ф and are quantized in terms of the Hopf indices and Brouwer degrees. The C-mapping topological current theory also provides a reasonable way to study the bifurcation theory of vortex lines in the two-dimensional Gross-Pitaevskii theory.展开更多
An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations....An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations. In the TGMS lines, fertile pollens had an average diameter of 0.89 mm while the sterile pollens was with 0.02 mm diameter.TS-29-150GY produced the biggest fertile pollens with 0.92 mm and other two lines produced relatively smaller pollens with 0.91 and 0.85 ram. Pollen fertility during the fertility reversion period was an average of 60.7%. TS-29-150GY had the maximum of 66.9% spikelet fertility whereas other two lines (TNAU18S and TNAU60S) had relatively lower spikelet fertility of 27.8% and 26.7%, respectively. Average of 17.00 g of seed yield was obtained in the TGMS lines during the fertility reversion period. TS-29- 150GY had the highest value of 21.20 g of seed yield while TNAU18S and TNAU60S produced 16.6 g and 13.2 g of seed yield, respectively. The low seed production ability of these three TGMS lines was attributed only to the environmental conditions prevailing during the period. All three TGMS lines had considerable outcrossing potential of 41.2%, 24.6% and 25.0%, respectively. The cross combinations viz. TNAU18S/IET21508 (36 g/plant), TNAU18S/IET21044 (13 g/plant), TNAU18S/IET21009 (26.5 g/plant), TNAU60S/CB-09-106 (26.2 g/plant), TNAU60S/IET21009 (14 g/plant) and TS29-150-GY/DRR 3306 (39.2 g/plant) showed perfect synchronization with acceptable hybrid seed yield, indicating suitability of TGMS system under Indian condition. Based on the outcrossing related traits viz. panicle exertion, angle of glume opening, stigma length and pollen size, TNAU18S was identified as the best, followed by TS-29-150GY.展开更多
A plant expression vector containing a chimeric Bt29K gene coding for the activated Cry1Ac protein and the arrowhead proteinase inhibitior gene API B were introduced into the cotton cultivar Jihe321 mediated ...A plant expression vector containing a chimeric Bt29K gene coding for the activated Cry1Ac protein and the arrowhead proteinase inhibitior gene API B were introduced into the cotton cultivar Jihe321 mediated by Agrobactertium tumefaciens. Based on the results of kanamycin resistant testing, PCR detection for both foreign genes and insect bioassay using Heliethis armigera , nine transgenic homozygous cotton lines with insect resistance of more than 90% and better agronomic traits were bred through six generations from the original transgenic plants. Results from insect bioassay and sequence analysis of the PCR products of plants from some homozygous lines indicated that the chimeric Bt29K gene was stably inherited in these transgenic cotton lines. The main agronomic characters of these homozygous cotton lines, such as boll productivity and fibre strength, were better than that of the original cotton cv. Jihe321.展开更多
The gluten proteins of wheat grain are responsible for visco-elastic properties of flour,but they also trigger the immune-response of celiac disease.In this work,two low-gliadin RNA interference(RNAi)wheat lines that ...The gluten proteins of wheat grain are responsible for visco-elastic properties of flour,but they also trigger the immune-response of celiac disease.In this work,two low-gliadin RNA interference(RNAi)wheat lines that differ for the promoter driving the silencing(D-hordein andγ-gliadin promoters for D783 and D793 lines,respectively),were characterized at transcriptomic,and protein fraction levels in the grain.Silencing of gliadins provides a readjustment in the grain protein fractions that also affects to the nongluten proteins(NGP),which were increased in both RNAi lines.Determination of wheat gluten by means of the R5 monoclonal antibody also showed a strong reduction in the content of gluten in both RNAi lines.Moreover,fructans,an oligosaccharide linked with the development of non-celiac wheat sensitivity(NCWS)were also significantly decreased in RNAi lines.The down-regulation of gliadins fractions also impacts to other metabolic processes,particularly on carbohydrate metabolism,enzyme regulator activity and response to stress.Genes and transcription factors regulated by ABA were up-regulated,which could suggest the implication of this phytohormone on the stress response observed in the RNAi lines.展开更多
The objective of the research discussed in this paper has been to find an optimal schedule on mixed mass production lines of two and three machines. Johnson's rule on flow shops is generalized to mixed mass produ...The objective of the research discussed in this paper has been to find an optimal schedule on mixed mass production lines of two and three machines. Johnson's rule on flow shops is generalized to mixed mass production lines. Scheduling methods on three-machine lines are proposed for six special kinds of data of processing times of jobs. The scheduling method on two-machine lines is also proposed.展开更多
Objective To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods Suppression subtractive hybridization (SSH) was used to createa subtracted lib...Objective To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods Suppression subtractive hybridization (SSH) was used to createa subtracted library, and clones were sequenced. Results Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs (Expressed Sequence Tag), and were collected by GenBank dbEST database (The access number was EB390424-7). Conclusion SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different cells may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.展开更多
An accurate numerical algorithm for three-line fault involving different phases from each of two-parallel lines is presented. It is based on one-terminal voltage and current data. The loop and nodel equations comparin...An accurate numerical algorithm for three-line fault involving different phases from each of two-parallel lines is presented. It is based on one-terminal voltage and current data. The loop and nodel equations comparing faulted phase to non-faulted phase of two-parallel lines are introduced in the fault location estimation modal, in which the faulted impedance of remote end is not involved. The effect of load flow and fault resistance on the accuracy of fault location are effectively eliminated, therefore an accurate algorithm of locating fault is derived. The algorithm is demonstrated by digital computer simulations and the results show that errors in locating fault are less than 1%.展开更多
Objective: To study apoptosis induced by radiation in two nasopharyngeal carcinoma (NPC) cell lines, CNE and CNE-2. Methods: Hoechst 33342 staining, immuno-histochemical staining, RT-PCR, DNA dot blotting and Southern...Objective: To study apoptosis induced by radiation in two nasopharyngeal carcinoma (NPC) cell lines, CNE and CNE-2. Methods: Hoechst 33342 staining, immuno-histochemical staining, RT-PCR, DNA dot blotting and Southern blotting were used to identify apoptosis. Results: A single dose of X-irradiation resulted in apoptosis, the apoptotic index (AI) was time- and dose-dependent. Different apoptotic responses existed in the two cell lines. Immunohistochemical staining showed that bcl-2 protein was strongly positive in CNE but negative in CNE-2. However, RT-PCR revealed p53 mRNA in CNE-2 but not in CNE. P53 and bcl-2 genes were both present in the two cell lines as shown by DNA blotting, but the 2.8 kb fragment of the p53 gene was much lower than the 5.6 kb fragment on CNE which was clearly shown in Southern hybridization, suggestive of partial deletion of p53 gene in CNE. Conclusion: Apoptotic response to radiation is different in two NPC cell lines. CNE is more radioresistant than CNE-2. Overexpression of bcl-2 protein and partial deletion of p53 gene may explain their difference in radiosensitivity.展开更多
The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes (Temminck et. Schlegel) as ...The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes (Temminck et. Schlegel) as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.展开更多
This paper attempts to investigate the problem for the interaction between a uniformly moving screw dislocation and interface rigid lines in two dissimilar.anisotropic. materials. Integrating Riemann-Schwarz's symmet...This paper attempts to investigate the problem for the interaction between a uniformly moving screw dislocation and interface rigid lines in two dissimilar.anisotropic. materials. Integrating Riemann-Schwarz's symmetry principle with the analysis singularity of complex functions, we present the general elastic solutions of this problem and the closed form solutions for interfaces containing one and two rigid lines. The expressions of stress intensity factors, at the rigid line tips and image force acting on moving dislocation are derived explicitly. The results show that dislocation velocity has an antishielding effect on the rigid line tip and a larger dislocation velocity leads to the equilibrium position of dislocation closing with the rigid line. The presented solutions contain previously known results as the special cases.展开更多
基金supported by the National Natural Science Foundation of China(32171990 and 32072053)Key Research and Development Program of Zhenjiang(NY2021001)+4 种基金State Key Laboratory of Plant Cell and Chromosome Engineering(PCCE-KF-2021-05 and PCCE-KF-2022-07)State Key Laboratory of Crop Biology in Shandong Agricultural University(2021KF01)Natural Science Foundation of the Jiangsu Higher Education institutions of China(21KJB210004)Open Project Funding of State Key Laboratory of Crop Stress Adaptation and Improvement(CX1130A0920014)Key Research and Development Program of Shandong Province(2020CXGC010805).
文摘Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).
文摘In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of systems with a center in two zones and without equilibrium points in the other zone (or with a center in one zone and without equilibrium points in the other zones). In addition, we also give examples to illustrate that it can reach the maximal number.
基金the support provided by the National Natural Science Foundation of China(NSFC,Grant Nos.12090040/3,12125303,12288102,and 11733008)the National Key Research and Development Program of China(grant No.2021YFA1600401/3)+3 种基金the China Manned Space Project(CMSCSST-2021-A10)the Yunnan Fundamental Research Projects(grant No.202101AV070001)the National Natural Science Foundation of China and the Chinese Academy of Sciences,under grant No.U1831125the Research Program of Frontier Sciences,CAS(grant No.QYZDY-SSW-SLH007)。
文摘The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.
基金supported by a research grant from the Ministry of Education,Culture,Research and Technology(KEMENDIKBUD RISTEK)number NKB-022/UN2.RST/HKP.05.00/2021 awarded to AB.
文摘Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.
基金Supported by General Program of National Natural Science Foundation of China,No.81770197Scientific and Technological Research Major Program of Chongqing Municipal Education Commission,No.KJZD-M202312802+1 种基金Chongqing Natural Science Foundation of China,No.CSTB2022NSCQ-MSX0190,No.CSTB2022NSCQ-MSX0176,and No.cstc2020jcyj-msxmX0051Xinqiao Young Postdoc Talent Incubation Program,No.2022YQB098.
文摘BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.
基金Tibet Autonomous Region Natural Fund Key Project(XZ202201ZR0024G)。
文摘The 10kV distribution network is an essential component of the power system,and its stable operation is crucial for ensuring reliable power supply.However,various factors can lead to faults in the distribution network.In order to enhance the safety and reliability of power distribution,this paper focuses on the analysis of faults in the 10kV distribution network caused by natural factors,operational factors,human factors,and equipment factors.It elucidates the various hazards resulting from distribution network faults and proposes corresponding preventive measures for different types of faults in the 10kV distribution network.The aim is to mitigate or reduce the impact of distribution network faults,ensuring the safe and stable operation of the distribution system.
文摘In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.
基金Supported by National Natural Science Foundation of China(Grant Nos.51275366,50875190,51305311)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.20134219110002)
文摘Due to the NP-hardness of the two-sided assembly line balancing (TALB) problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In this paper, an effective hybrid algorithm is proposed to address the TALB problem with multiple constraints (TALB-MC). Considering the discrete attribute of TALB-MC and the continuous attribute of the standard teaching-learning-based optimization (TLBO) algorithm, the random-keys method is hired in task permutation representation, for the purpose of bridging the gap between them. Subsequently, a special mechanism for handling multiple constraints is developed. In the mechanism, the directions constraint of each task is ensured by the direction check and adjustment. The zoning constraints and the synchronism constraints are satisfied by teasing out the hidden correlations among constraints. The positional constraint is allowed to be violated to some extent in decoding and punished in cost fimction. Finally, with the TLBO seeking for the global optimum, the variable neighborhood search (VNS) is further hybridized to extend the local search space. The experimental results show that the proposed hybrid algorithm outperforms the late acceptance hill-climbing algorithm (LAHC) for TALB-MC in most cases, especially for large-size problems with multiple constraints, and demonstrates well balance between the exploration and the exploitation. This research proposes an effective and efficient algorithm for solving TALB-MC problem by hybridizing the TLBO and VNS.
基金This work was financially supported by the National Natural Science Foundation of China(Grant No.50009005 and No.50039010)
文摘In this paper, the motions are studied of a multi-body which is composed of two plates hinged together and moored by eight mooring lines in regular waves. The experimental results are compared with computational results. The linear potential theory and the perturbation method are combined to study this complicated system. The former is used to calculate the wave forces acting on the plates and the motion responses of them, while the latter is used to describe the dynamic character of the eight mooring lines coupled with the two hinged plates. Some response results of each plate are presented and comparisons between calculated results and experimental data are given. All the calculations are confined to regular beam waves.
基金financially supported by the National Key Research and Development Program(2017YFD0600101)the Central University Basic Research and Operating Expenses of Special Funding(2572016CB04)the Harbin Application Technology Research and Development Projects(2016RQQXJ134)
文摘Electrical pollution is a worldwide concern,because it is potentially harmful to human health.Trees not only play a significant role in moderating the climate,but also can be used as shields against electrical pollution.Shielding effects on the electric field strength under transmission lines by two tree species,Populus alba and Larix gmelinii,were examined in this study.The electrical resistivity at different heights of trees was measured using a PiCUS sonic tomograph,which can image the electrical impedance for trees.The electric field strength around the trees was measured with an elf field strength measurement system,HI-3604,and combined with tree resistivity to develop a model for calculating the electric field intensity around trees using the finite element method.In addition,the feasibility of the finite element method was confirmed by comparing the calculated results and experimental data.The results showed that the trees did reduce the electric field strength.The electric field intensity was reduced by 95.6%,and P.alba was better than L.gmelinii at shielding.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 10905026 and 10905027)the Program of Science and Technology Development of Lanzhou, China (Grant No. 2010-1-129)
文摘Using the C-mapping topological theory, we study the topological structure of vortex lines in a two-dimensional generalized Gross Pitaevskii theory in (3+l)-dimensional space-time. We obtain the reduced dynamic equation in the framework of the two-dimensional Gross-Pitaevskii theory, from which a conserved dynamic quantity is derived on the stable vortex lines. Such equations can also be used to discuss Bose-Einstein condensates in heterogeneous and highly nonlinear systems. We obtain an exact dynamic equation with a topological term, which is ignored in traditional hydrodynamic equations. The explicit expression of vorticity as a function of the order parameter is derived, where the function indicates that the vortices can only be generated from the zero points of Ф and are quantized in terms of the Hopf indices and Brouwer degrees. The C-mapping topological current theory also provides a reasonable way to study the bifurcation theory of vortex lines in the two-dimensional Gross-Pitaevskii theory.
文摘An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations. In the TGMS lines, fertile pollens had an average diameter of 0.89 mm while the sterile pollens was with 0.02 mm diameter.TS-29-150GY produced the biggest fertile pollens with 0.92 mm and other two lines produced relatively smaller pollens with 0.91 and 0.85 ram. Pollen fertility during the fertility reversion period was an average of 60.7%. TS-29-150GY had the maximum of 66.9% spikelet fertility whereas other two lines (TNAU18S and TNAU60S) had relatively lower spikelet fertility of 27.8% and 26.7%, respectively. Average of 17.00 g of seed yield was obtained in the TGMS lines during the fertility reversion period. TS-29- 150GY had the highest value of 21.20 g of seed yield while TNAU18S and TNAU60S produced 16.6 g and 13.2 g of seed yield, respectively. The low seed production ability of these three TGMS lines was attributed only to the environmental conditions prevailing during the period. All three TGMS lines had considerable outcrossing potential of 41.2%, 24.6% and 25.0%, respectively. The cross combinations viz. TNAU18S/IET21508 (36 g/plant), TNAU18S/IET21044 (13 g/plant), TNAU18S/IET21009 (26.5 g/plant), TNAU60S/CB-09-106 (26.2 g/plant), TNAU60S/IET21009 (14 g/plant) and TS29-150-GY/DRR 3306 (39.2 g/plant) showed perfect synchronization with acceptable hybrid seed yield, indicating suitability of TGMS system under Indian condition. Based on the outcrossing related traits viz. panicle exertion, angle of glume opening, stigma length and pollen size, TNAU18S was identified as the best, followed by TS-29-150GY.
文摘A plant expression vector containing a chimeric Bt29K gene coding for the activated Cry1Ac protein and the arrowhead proteinase inhibitior gene API B were introduced into the cotton cultivar Jihe321 mediated by Agrobactertium tumefaciens. Based on the results of kanamycin resistant testing, PCR detection for both foreign genes and insect bioassay using Heliethis armigera , nine transgenic homozygous cotton lines with insect resistance of more than 90% and better agronomic traits were bred through six generations from the original transgenic plants. Results from insect bioassay and sequence analysis of the PCR products of plants from some homozygous lines indicated that the chimeric Bt29K gene was stably inherited in these transgenic cotton lines. The main agronomic characters of these homozygous cotton lines, such as boll productivity and fibre strength, were better than that of the original cotton cv. Jihe321.
基金funded by the Spanish Ministry of Science and Innovation(Project PID2019-110847RB-I00)the European Regional Development Fund(FEDER)。
文摘The gluten proteins of wheat grain are responsible for visco-elastic properties of flour,but they also trigger the immune-response of celiac disease.In this work,two low-gliadin RNA interference(RNAi)wheat lines that differ for the promoter driving the silencing(D-hordein andγ-gliadin promoters for D783 and D793 lines,respectively),were characterized at transcriptomic,and protein fraction levels in the grain.Silencing of gliadins provides a readjustment in the grain protein fractions that also affects to the nongluten proteins(NGP),which were increased in both RNAi lines.Determination of wheat gluten by means of the R5 monoclonal antibody also showed a strong reduction in the content of gluten in both RNAi lines.Moreover,fructans,an oligosaccharide linked with the development of non-celiac wheat sensitivity(NCWS)were also significantly decreased in RNAi lines.The down-regulation of gliadins fractions also impacts to other metabolic processes,particularly on carbohydrate metabolism,enzyme regulator activity and response to stress.Genes and transcription factors regulated by ABA were up-regulated,which could suggest the implication of this phytohormone on the stress response observed in the RNAi lines.
文摘The objective of the research discussed in this paper has been to find an optimal schedule on mixed mass production lines of two and three machines. Johnson's rule on flow shops is generalized to mixed mass production lines. Scheduling methods on three-machine lines are proposed for six special kinds of data of processing times of jobs. The scheduling method on two-machine lines is also proposed.
基金This work was supported by the National Natural Science Foundation of China (No30370660)
文摘Objective To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods Suppression subtractive hybridization (SSH) was used to createa subtracted library, and clones were sequenced. Results Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs (Expressed Sequence Tag), and were collected by GenBank dbEST database (The access number was EB390424-7). Conclusion SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different cells may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.
文摘An accurate numerical algorithm for three-line fault involving different phases from each of two-parallel lines is presented. It is based on one-terminal voltage and current data. The loop and nodel equations comparing faulted phase to non-faulted phase of two-parallel lines are introduced in the fault location estimation modal, in which the faulted impedance of remote end is not involved. The effect of load flow and fault resistance on the accuracy of fault location are effectively eliminated, therefore an accurate algorithm of locating fault is derived. The algorithm is demonstrated by digital computer simulations and the results show that errors in locating fault are less than 1%.
文摘Objective: To study apoptosis induced by radiation in two nasopharyngeal carcinoma (NPC) cell lines, CNE and CNE-2. Methods: Hoechst 33342 staining, immuno-histochemical staining, RT-PCR, DNA dot blotting and Southern blotting were used to identify apoptosis. Results: A single dose of X-irradiation resulted in apoptosis, the apoptotic index (AI) was time- and dose-dependent. Different apoptotic responses existed in the two cell lines. Immunohistochemical staining showed that bcl-2 protein was strongly positive in CNE but negative in CNE-2. However, RT-PCR revealed p53 mRNA in CNE-2 but not in CNE. P53 and bcl-2 genes were both present in the two cell lines as shown by DNA blotting, but the 2.8 kb fragment of the p53 gene was much lower than the 5.6 kb fragment on CNE which was clearly shown in Southern hybridization, suggestive of partial deletion of p53 gene in CNE. Conclusion: Apoptotic response to radiation is different in two NPC cell lines. CNE is more radioresistant than CNE-2. Overexpression of bcl-2 protein and partial deletion of p53 gene may explain their difference in radiosensitivity.
基金The finance special program of Tianjinthe transformation project of Tianjin Agricultural Achievements
文摘The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes (Temminck et. Schlegel) as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.
基金the National Natural Science Foundation of China(No.10472030)
文摘This paper attempts to investigate the problem for the interaction between a uniformly moving screw dislocation and interface rigid lines in two dissimilar.anisotropic. materials. Integrating Riemann-Schwarz's symmetry principle with the analysis singularity of complex functions, we present the general elastic solutions of this problem and the closed form solutions for interfaces containing one and two rigid lines. The expressions of stress intensity factors, at the rigid line tips and image force acting on moving dislocation are derived explicitly. The results show that dislocation velocity has an antishielding effect on the rigid line tip and a larger dislocation velocity leads to the equilibrium position of dislocation closing with the rigid line. The presented solutions contain previously known results as the special cases.