Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded ...Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress re-sponses and controlled by an ABA-dependent signaling pathway.展开更多
Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays imp...Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays important roles in trehalose metabolism and signaling.In this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7.However,the function of At TPS7 has not been determined.To analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in T.hispida under salt and drought stress and JA,ABA and GA3 hormone stimulation was monitored by qRT-PCR.The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused particularly strong induction of Th TPS in the roots of T.hispida.The ThTPS gene was transiently overexpressed in T.hispida.Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in T.hispida.Overall,the Th TPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic tolerance.These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.展开更多
[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectivel...[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.展开更多
基金This study was supported by national natural science foundation (Grant No. 30972386)Central university basic scientific business specific foundation (Grant No. DL09BA22)Genetically modified organisms breeding major projects (Grant No.2009ZX08009-098B)
文摘Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress re-sponses and controlled by an ABA-dependent signaling pathway.
基金supported by the Province in Heilongjiang Outstanding Youth Science Fund(JC2017004)the National Natural Science Foundation of China(No.31370676)Heilongjiang Touyan Innovation Team Program(Tree Genetics and Breeding Innovation Team)。
文摘Trehalose is a non-reducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses.Trehalose-6-phosphate synthase(TPS)plays important roles in trehalose metabolism and signaling.In this study,the full-length cDNA of ThTPS was cloned from Tamarix hispida Willd.A phylogenetic tree including ThTPS and 11 AtTPS genes from Arabidopsis indicated that the ThTPS protein had a close evolutionary relationship with AtTPS7.However,the function of At TPS7 has not been determined.To analyze the abiotic stress tolerance function of ThTPS,the expression of ThTPS in T.hispida under salt and drought stress and JA,ABA and GA3 hormone stimulation was monitored by qRT-PCR.The results show that ThTPS expression was clearly induced by all five of these treatments at one or more times,and salt stress caused particularly strong induction of Th TPS in the roots of T.hispida.The ThTPS gene was transiently overexpressed in T.hispida.Both physiological indexes and staining results showed that ThTPS gene overexpression increased salt and osmotic stress tolerance in T.hispida.Overall,the Th TPS gene can respond to abiotic stresses such as salt and drought,and its overexpression can significantly improve salt and osmotic tolerance.These findings establish a foundation to better understand the responses of TPS genes to abiotic stress in plants.
基金Supported by College Students Innovative Experimental Project of Northeast Forestry University(No.201210225004)
文摘[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.
