Mufangji tang ameliorates pulmonary arterial hypertension through improving vascular remodeling,inhibiting inflammatory response and oxidative stress,and inducing apoptosis

Background:Mufangji tang(MFJT)is composed of Ramulus Cinnamomi,Radix Ginseng,Cocculus orbiculatus(Linn.)DC.,and Gypsum.In clinical settings,MFJT has been effectively employed in addressing a range of respiratory disorders,notably including pulmonary arterial hypertension(PAH).However,the mechanism of action of MFJT on PAH remains unknown.Methods:In this study,a monocrotaline-induced PAH rat model was established and treated with MFJT.The therapeutic effects of MFJT on PAH rat model were evaluated.Network pharmacology was conducted to screen the possible targets for MFJT on PAH,and the molecular docking between the main active components and the core targets was carried out.The key targets identified from network pharmacology were tested.Results:Results showed significant therapeutic effects of MFJT on PAH rat model.Analysis of network pharmacology revealed several potential targets related to apoptosis,inflammation,oxidative stress,and vascular remodeling.Molecular docking showed that the key components were well docked with the core targets.Further experimental validation results that MFJT treatment induced apoptosis(downregulated Bcl-2 levels and upregulated Bax levels in lung tissue),inhibited inflammatory response and oxdative stress(decreased the levels of IL-1β,TNF-α,inducible NOS,and malondialdehyde,and increased the levels of endothelial nitric oxide synthase,nitric oxide,glutathione and superoxide dismutase),reduced the proliferation of pulmonary arterial smooth muscle cells(downregulated ET-1 andβ-catenin levels and ERK1/2 phosphorylation,increased GSK3βlevels).Conclusion:Our study revealed MFJT treatment could alleviate PAH in rats via induction of apoptosis,inhibition of inflammation and oxidative stress,and the prevention of vascular remodeling.

Gaze and Oppositional Gaze in Mr. Tang’s Girls

The gaze is a means of power that can flow through family relationships.It has intense regulatory overtones.In her short story Mr.Tang’s Girls,Malaysian-Chinese Anglophone diasporic writer Shirley Geok-lin Lim shows the conflict between Eastern and Western cultures and the resistance of women under the oppression of patriarchy through the portrayal of the father,Ah Kong,and the eldest daughter,Kim Li.Based on Foucault’s discipline gaze and Hooks’oppositional gaze,this article analyzes the power operation in the story from the following three aspects:the male gaze,discipline and self-discipline,and the oppositional gaze.Kim Li’s subjective image of breaking free from male ownership control has been presented.It suggests the difficulties encountered by female groups in their rebellion against the male gaze and their pursuit of freedom and emancipation.It also reflects the tragedy of awakening women’s destinies under the dual oppression of patriarchy and the East-Westen cultural conflict.

Clinical efficacy of external treatment with Zi Cao Yang He Tang combined with hormone therapy for granulomatous mastitis and its effect on cellular pyroptosis proteins

Objective:To observe the clinical effect of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in the treatment of granulomatous lobular mastitis in the mass stage and the effect on cellular pyroptosis-related proteins.Methods:Forty patients with granulomatous lobular mastitis were randomly divided into 20 cases in the observation group and 20 cases in the control group.Both groups were given dexamethasone ointment for treatment,and the observation group was treated with external application of Zi Cao Yang He Tang on the basis of the control group.Breast lump vol-ume changes,breast pain and pus symptoms,pathology and histology and immunohistochemistry,cellular death-related indicators expression were compared between the two groups.Results:After treatment,the difference in mass volume of the observation group was significantly greater than that of the control group(16.7±21.5 in the control group and 32.9±27.4 in the experimental group)and the difference between the two groups was statistically significant(P<0.05);the objective remission rate of the patients in the observation group(ORR=85%)was significantly higher than that of the treatment group(ORR=50%,P<0.05).In terms of pus formation rate and pain level,the breast pain score of the observation group was significantly lower than that of the control group after two weeks or three weeks of treatment(P<0.05);the pus formation rate of the observation group was 30%,and the pus formation rate of the control group was 20%,and the difference was not statistically significant(P>0.05).The immunohistochemical expression levels of caspase-1 and GSDMD in the observation group were significantly lower than those in the control group(P<0.05);and in terms of the expression of cellular pyroptosis-related indexes,the expression of GSDMD,cas-pase-1,and NLRP3 in the observation group was significantly lower compared with that in the control group(P<0.05).Conclusion:The efficacy of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in treating granulomatous lobular mastitis in the mass stage is remarkable,which can effectively reduce breast pain,reduce the size of the mass,de-crease the expression of cellular pyroptosisrelated indexes,and have a good anti-inflammatory effect.

Effects of Xiaoxianxiong Tang on biological behavior in lung cancer cell line A549

Obiective:To investigate the effects of Xiaoxianxiong Tang on the proliferation,invasion,apoptosis,and other cell biological behaviors of non-small cell lung cancer A549 cells.Methods:Human lung adenocarcinoma A549 cells were cultured in vitro,and the IC50 concentration and effective time of administration of Xiaoxianxiong Tang were determined by the CCK-8 assay to detect the inhibitory effect of Xiaoxianxiong Tang on A549 cells proliferation.The effect of the Xiaoxianxiong Tang on apoptosis was determined by flow cytometry;the apoptosis-related protein was detected via Western blot;the metastasis-related protein mRNA was detected by RT-PCR.Results:Xiaoxianxiong Tang significantly inhibited the proliferation viability,the invasive ability,and the clonogenic ability of A549 cells compared with the control group(P<0.001).Moreover,Xiaoxianxiong Tang significantly promoted the apoptosis of A549 cells(P<0.001).Xiaoxianxiong Tang significantly up-regulated Bax and down-regulated Bcl2 expression in A549 cells compared with the control group(P<0.01).The mRNA expression of MMP2 and MMP9 was significantly down-regulated by Xiaoxianxiong Tang compared with the control group(P<0.05).Conclusion:Xiaoxianxiong Tang has the effect of regulating the biological behavior of A549 cells,and Xiaoxianxiong Tang significantly inhibites the proliferation viability,colony formation,and invasion ability of lung cancer A549 cells.

Study on the mechanism of action of Lizhong Tang in ameliorating non-alcoholic fatty liver disease by regulating mitochondrial autophagy

Objective:To investigate the therapeutic efficacy and underlying mechanisms of LiZhong Tang in the context of non-alcoholic fatty liver disease(NAFLD).Methods:High-fat feed was used to induce NAFLD in rats,Blood and liver samples were collected to facilitate a comparative analysis of rat body mass and liver wet weight and calculate the liver index.Liver pathology was observed,while serum transaminase and blood lipid levels were measured.The protein expression levels of PINK1,Parkin,and LC-3II in rat liver were detected using Western Blot analysis.Results:Compared with the control group,the NAFLD rats exhibited a significant increase in body weight,liver wet weight,liver index,transaminase levels,and blood lipid levels.The expression levels of PINK1,Parkin,and LC3-II protein were significantly decreased(P<0.01).Following intervention with Lizhong Tang,rats in each herbal treatment group displayed a decrease in body weight,liver wet weight,liver index,se-rum transaminase,and blood lipid levels.The expression levels of PINK1,Parkin,and LC-3II rebounded(P<0.05),with the high-dose group demonstrating the most pronounced effects(P<0.01).Histopathological examination of liver tissue revealed that rats in the model group displayed disrupted hepatic lobule structure,swollen hepatocytes,disordered arrangement,and a multi-tude of varying-sized lipid vacuoles within the cytoplasm.Conversely,rats treated with different doses of the herbal remedy exhibited improvements in liver tissue pathology,with the high-dose group showing the most notable enhancement.Conclusion:Lizhong Tang can improve NAFLD disease by regulating mitochondrial autophagy.

A Study on the“Social Behavior”of Zen Poetry Culture:A Case Study of the Sui and Tang Dynasties

During the Sui and Tang dynasties,Buddhism and Zen poetry underwent great development.Zen poetry is diverse in form,strong in personality,and high in achievement.Among the types of Zen poetry are poems,lyrics,and prose,some of which have social attributes closely related to the development of Chinese Buddhism.An analysis reveals that the viewpoints and information conveyed in these poems,lyrics,and prose are positively correlated with the attitudes of the emperors towards Buddhism.When the attitude of the emperor towards Buddhism reached its peak of positivity during the Tang dynasty,there was a change towards a negative correlation with the viewpoints expressed in poetry.

M-Tang法肌腱缝合技术的生物力学研究及其在Ⅱ区屈肌腱修复中的应用

目的报道一种新的肌腱缝合方法 M-Tang法的生物力学特性及临床应用结果。方法本研究采用36根猪后足屈肌腱作为实验材料,在相当于Ⅱ区水平造成切割伤后,18根用Tang法、18根用M-Tang法进行修复。将修复后的肌腱分别进行直线和90°成角状态下的拉伸,用Instron力学测定仪测定2 mm间隙形成负荷及断裂负荷。M-Tang法用于临床修复Ⅱ区屈肌腱65例共96指,术后均采用保护性主、被动活动相结合的锻炼计划。采用Strickland标准进行功能评价。结果在直线拉伸模式下,M-Tang法的2 mm间隙形成负荷为(46.2±5.2)N,断裂负荷为(61.9±6.0)N,与Tang法相近;在90°成角拉伸模式下,M-Tang法的2 mm间隙形成负荷为(35.9±3.6)N,与Tang法相近,断裂负荷为(57.0±4.5)N,高于Tang法。65例患者术后平均随访26个月,临床运用M-Tang法修复的Ⅱ区屈肌腱无1例发生断裂,根据Strickland TAM标准,其中优78指,良10指,可8指,优良率91.6%。结论 M-Tang法具备Tang法的生物力学强度,操作简便,使用缝线和外露线结少,能满足肌腱早期保护性主动活动的需要,是Ⅱ区屈肌腱修复的优选方法之一。

Investigation of the mechanism of action of Sanwu Huangqin Tang in the treatment of restless leg syndrome based on network pharmacology,molecular docking technology and molecular dynamics simulation

Background:Through the use of network pharmacology and molecular docking approaches,this study will examine the pharmacological effects of Sanwu Huangqin Tang on restless legs syndrome in order to better understand the mechanism of action of Traditional Chinese medicine(TCM)on RLS.Method:Utilise the TCMSP database to collect and select the drug components of Sanwu Huangqin Tang,and the Uniprot database to identify pertinent targets;RLS-related disease targets were obtained from GeneCards,DrugBank,and OMIM databases;and STRING and Cytoscape 3.9.1 software were used to generate an interaction network.KEGG pathway analysis and GO enrichment analysis were performed utilizing the DAVID database.Use Autodock for analyzing the relationships between targets and core components.Result:By using a network pharmacology approach,83 active ingredients and 50 drug-disease intersecting targets were derived for Sanwu Huangqin Tang.The molecular docking results showed that the drug components had strong affinity with the average target targets.Conclusions:This study provides new insights into the mechanism through which Traditional Chinese Medicine(TCM)treats Restless Legs Syndrome(RLS).Furthermore,it lays the foundation for additional research into the mechanism of treatment for RLS through the intervention that addresses various targets and pathways using the active ingredients identified by Sanwu Huangqin Tang.

Exploration of the mechanism of action of Xiayuxue Tang against hepatic fibrosis based on GEO data mining,network pharmacology and molecular docking technology

Objective:To study the mechanism of action of Xiayuxue Tang in treating hepatic fibrosis by combining GEO data mining,network pharmacology,and molecular docking technology,and provide new research directions for the treatment of hepatic fibrosis.Method:Utilizing multiple databases,we aim to identify the relevant targets of various components in Xiayuxue Tang and their associations with hepatic fibrosis.After pinpointing the key targets through interaction analysis,we will construct both the compound-target network and the protein interaction network for Xiayuxue Tang.Conclusively,we will conduct GO and KEGG enrichment analyses on these key targets,followed by molecular docking verification.Result:Through mining the GEO database,171 related targets were identified.When combined with other databases,a total of 2,343 hepatic fibrosis-related targets were obtained.Xiayuxue Tang comprises 82 related components,which include 26 active components from rhubarb,1 from ground beetle worm,46 from peach kernels,with a total of 314 predicted targets.The GO enrichment analysis revealed 748 biological processes,32 cellular components,and 73 molecular functions,while the KEGG enrichment analysis identified 222 pathways.Molecular docking verification confirmed that effective compounds can bind stably to key proteins,exhibiting strong binding activity.This underscores the potential efficacy of Xiayuxue Tang in addressing hepatic fibrosis.Conclusion:Xiayuxue Tang exerts regulatory effects on hepatic fibrosis through different targets and pathways,suggesting that the herbal compound has the characteristics of multiple pathways and targets.

基于TANG模型的西安国际旅游成长实证分析

文章运用旅游地非线性成长模型(TANG模型)对50年来西安国际旅游成长进行实证分析。分析指出,西安国际旅游第一周期为新中国成立至改革开放前阶段,规模极小,受政府制度、资源与产品创新、大规模突发事件影响极大;从客流量与旅游收入两个指标体系入手,讨论1978年以来的第二、第三周期,通过模拟方程、成长曲线及相关特征值实现了不同周期、不同指标的横向与纵向比较,同时在与统计值进行对比的过程中,清晰地反映出异常波动情况,判断波动原因,对旅游地成长过程有了更为清晰的认识。

关于“门光尺”——答英国Mr.H·W·Tang

本刊英国读者邓孔怀先生(Mr.H·W·Tang)在《古建园林技术》总第六期研读了路长先生的《门光尺》一文(见总第六期第55～56页),甚感兴趣。邓先生参考《鲁班经》、《中国古代建筑技术史》(以下简称《技术史》)、清工部《工程做法则例》(以下简称《则例》)等文献资料。

On C-E Translation of Chinese Tang Poetry from the Perspective of Ecotranslatology

Tang poetry is a shining pearl in the history of Chinese literature.C-E translation of Tang poetry is regarded as a very complex and hard task because of its special style and form,concise diction and profound artistic conception.Eco-translatology provides a new perspective for the study on C-E translation of Tang poetry.Based on analysis of some practical examples,this paper discusses some C-E translation methods of tang poetry from the perspective of eco-translatology,namely,adaptive transformation from linguistic,cultural and communicative dimensions.

On Rhyme and Symmetry in the Translation of Tang Poetry:A Case Study of Xu Yuanchong’s English Translation of 300 Tang Poems

Ancient poetry represents historical and cultural legacies accumulated by the Chinese nation for thousands of years and underpins the strong cultural confidence of the Chinese people.Tang poetry distinguishes itself from other literary works with its beautiful rhyme and symmetry.Previous studies of English translations of Tang poetry mostly focused on appreciation,analysis,and critics of different translation versions.Few researchers have delved into the specific translation methods for achieving the rhyming and symmetrical effects of Tang poetry in the target text.This paper examined Professor Xu Yuanchong’s English translation of 300 Tang Poems as the research object,and elaborated on how to retain the rhyming and symmetrical effects of Tang poems.This paper also summarized some practical methods that can be applied to translating Tang poems.Rhyming can be realized by the addition of end rhyme words and changes in word or line order.Symmetry can be achieved by the omission of a modifier or predicate,the addition of function words,changes in word order,or parallelism with the original sentence structure.

Tang缝合法修复指屈肌腱的疗效分析

目的:探讨Tang缝合法在手指屈肌腱断裂治疗中的效果。方法:回顾性分析手指屈肌腱断裂患者96例临床资料,随机分为两组,Tang缝合法组48例(研究组)、Kessler缝合法组48例(对照组),分别采用Tang缝合法和Kessler缝合法修复手指屈肌腱,应用透明质酸钠填充治疗,24 h后开始手指功能锻炼,术后3周内采用Kleinert橡皮筋牵引疗法(动态支具保护),并定期随访,术后6个月手指功能采用主动活动度(Total active movement,TAM)法评定。结果:研究组TAM优良率为91.6%;对照组TAM优良率为54.1%,两组比较,差异有统计学意义(P<0.01)。结论:Tang缝合法在手指屈肌腱断裂治疗中获得满意的修复效果。

Effects of Wen Dan Tang on insomnia-related anxiety and levels of the brain-gut peptide Ghrelin

Ghrelin, a brain-gut peptide that induces anxiety and other abnormal emotions, contributes to the effects of insomnia on emotional behavior. In contrast, the traditional Chinese Medi- cine remedy Wen Dan Tang reduces insomnia-related anxiety, which may perhaps correspond to changes in the brain-gut axis. This suggests a possible relationship between Wen Dan Tan^s pharmacological mechanism and the brain-gut axis. Based on this hypothesis, a sleep-deprived rat model was induced and Wen Dan Tang was administered using oral gavage during model es- tablishment. Wen Dan Tang significantly reduced insomnia-related anxiety and prevented Ghrelin level decreases following sleep deprivation, especially in the hypothalamus. Increased expression of Ghrelin receptor mRNA in the hypothalamus was also observed, suggesting that reduced anxi- ety may be a result of Wen Dan Tan＇s regulation of Ghrelin-Ghrelin receptors.

Traditional Chinese medicine “Qing Yi Tang” alleviates oxygen free radical injury in acute necrotizing pancreatitis

ＴｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅｍｅｄｉｃｉｎｅ“ＱｉｎｇＹｉＴａｎｇ”ａｌｅｖｉａｔｅｓｏｘｙｇｅｎｆｒｅｅｒａｄｉｃａｌｉｎｊｕｒｙｉｎａｃｕｔｅｎｅｃｒｏｔｉｚｉｎｇｐａｎｃｒｅａｔｉｔｉｓＬＩＺｈａｎＬｉａｎｇ１，ＷＵＣｈｅｎｇＴａｎ...

金边阔叶麦冬(Liriope platyphylla Wang et Tangvar.variegata Hort.)的组织培养

将金边阔叶麦冬 (LiriopeplatyphyllaWangetTangvar.variegataHort.)不同部位外植体块 ,接种于附加不同激素配比的MS培养基上 ,实验结果表明 ,取自根状茎芽点 ,培养基为MS +BA 1.5mg/L +NAA 0 .5mg/L的不定芽诱导率最高 ( 10 0 % ) ,长势较旺 ,不定芽和愈伤组织均较多 ,但极易造成性状分离。用MS +BA 3.0mg/L +NAA 0 .5mg/L液体培养可以直接“芽生芽” ,避免了脱分化过程 ,从而稳定保持嵌合性。不定芽每月可转接 1次 ,适宜的生根培养基为 1/2MS大量 +IBA 0 .2 5mg/L。试管苗在蛭石中炼苗后即可移栽。

Effects of Lizhong Tang on gastrointestinal motility in mice

AIM To investigate the effects of Lizhong Tang,a traditional Chinese medicine formula,on gastrointestinal motility in mice.METHODS The in vivo effects of Lizhong Tang on GI motility were investigated by measuring the intestinal transit rates(ITRs) and gastric emptying(GE) values in normal mice and in mice with experimentally induced GI motility dysfunction(GMD).RESULTS In normal ICR mice,the ITR and GE values were significantly and dose-dependently increased by Lizhong Tang(ITR values: 54.4% ± 1.9% vs 65.2% ± 1.8%,P < 0.01 with 0.1 g/kg Lizhong Tang and 54.4% ± 1.9% vs 83.8% ± 1.9%,P < 0.01 with 1 g/kg Lizhong Tang; GE values: 60.7% ± 1.9% vs 66.8% ± 2.1%,P < 0.05 with 0.1 g/kg Lizhong Tang and 60.7% ± 1.9% vs 72.5% ± 1.7%,P < 0.01 with 1 g/kg Lizhong Tang). The ITRs of the GMD mice were significantly reduced compared with those of the normal mice,which were significantly and dose-dependently reversed by Lizhong Tang. Additionally,in loperamide- and cisplatin-induced models of GE delay,Lizhong Tang administration reversed the GE deficits.CONCLUSION These results suggest that Lizhong Tang may be a novel candidate for development as a prokinetic treatment for the GI tract.

Intervention effect of traditional Chinese medicine Yi Tang Kang on metabolic syndrome of spleen deficiency

Objective:To investigate effects of herbal compound Yi Tang Kang on the spleen deficiency metabolic syndrome.Methods:Forty male Wistar rats were randomly divided into two groups:the normal control group and the MS spleen deficiency syndrome group.The control group rats were fed with standard diet and water,while MS spleen deficiency syndrome group with high fat diet and low dose intraperitoneal injection of slreptozocin.which swam to the endurance limit.After 12 weeks,the MS spleen deficiency syndrome group was randomly divided into two groups,with 13 rats in each group.Flats in model group were fed with high fat diet and conlinuouly administered with daily saline,and rats in intervention group with high fat diet were trated with traditional Chinese medicines Yi Tang Kang by gavage,2 mL/200 g at the same lime every day.10 weeks later,the expression of serum proteomics was investigated through abdominal aortic puncture and separation of serum,using isotope labeling technique,high performance liquid chromatography and four bar-Orbitrap mass spectrometer.Results:After treatment with traditional Chinese medicine yitangkang,in the model group,important carboxylesterase and retinal guanylate cyclase 2 precursor were upregulated.As for intervention group,these indesxes were raised,but immunoglobulin IgG,carnitine acetyltransferase,tubulin beta-5,and Gan Lu sugar binding protein C were down-regulated.At the same time,some new biological active substances,such as protein tyrosine kinase,beta glucosidase were also found.Conclusions:Traditional Chinese medicines Yi Tang Kang could regulate glucose and lipid metabolism in rats with spleen deficiency syndrome.

Effects of Lizhong Tang on cultured mouse small intestine interstitial cells of Cajal

AIM:To investigate the effects of Lizhong Tang,an herbal product used in traditional Chinese medicine,on mouse small intestine interstitial cells of Cajal(ICCs).METHODS:Enzymatic digestions were used to dissociate ICCs from mouse small intestine tissues.The ICCs were morphologically distinct from other cell types in culture and were identified using phase contrast microscopy after verification with anti c-kit antibody.A whole-cell patch-clamp configuration was used to record potentials(current clamp) from cultured ICCs.All of the experiments were performed at 30-32 ℃.RESULTS:ICCs generated pacemaker potentials,and Lizhong Tang produced membrane depolarization in current-clamp mode.The application of flufenamic acid(a nonselective cation channel blocker) abolished the generation of pacemaker potentials by Lizhong Tang.Pretreatment with thapsigargin(a Ca 2+-ATPase inhibi-tor in the endoplasmic reticulum) also abolished the generation of pacemaker potentials by Lizhong Tang.However,pacemaker potentials were completely abolished in the presence of an external Ca 2+-free solution,and under this condition,Lizhong Tang induced membrane depolarizations.Furthermore,When GDPβ-S(1 mmol/L) was in the pipette solution,Lizhong Tang still induced membrane depolarizations.In addition,membrane depolarizations were not inhibited by chelerythrine or calphostin C,which are protein kinase C inhibitors,but were inhibited by U-73122,an active phospholipase C inhibitors.CONCLUSION:These results suggest that Lizhong Tang might affect gastrointestinal motility by modulating pacemaker activity in interstitial cells of Cajal.