Objective： To evaluate the renal protective effect of Tangshenkang Granule（糖肾康颗粒） in a rat model of diabetic nephropathy（DN）.Methods： Forty male Sprague-Dawley rats were randomly divided into control,DN,Tan...Objective： To evaluate the renal protective effect of Tangshenkang Granule（糖肾康颗粒） in a rat model of diabetic nephropathy（DN）.Methods： Forty male Sprague-Dawley rats were randomly divided into control,DN,Tangshenkang and benazepril groups.DN model was established in the rats of DN,Tangshenkang and benazepril groups.Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks,respectively.Urinary albumin and creatinine were detected.The albumin/creatinine（ACR） was calculated in addition to 24 h urinary protein（24-h UPr）,serum creatinine（Scr）,blood urea nitrogen（BUN）,total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL）,and creatinine clearance rate（Ccr）.Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining.The average glomerular diameter（DG）,average glomerular（AG） and mesangial areas（AM） were measured.The thickness of glomerular basement membrane（TGBM） was detected using transmission electron microscope.Results： Compared with rats in the control group,rats in the DN group showed significantly decreased body weight,increased hypertrophy index,24-h urinary volume,24-h UPr,ACR,Scr,BUN,Ccr,blood lipids as well as renal pathological indices including DG,AG,AM,AM/AG and TGBM（P〈0.05）.Compared with the DN group,the weights of rats in the Tangshenkang and benazepril groups were significantly increased,and the renal hypertrophy indices were significantly decreased（P 〈0.05）.The 24-h urinary volumes,ACR,24-h UPr,Scr,BUN,Ccr,LDL,DG,AG,AM and TGBM were obviously decreased（P 〈0.05）.Compared with the benazepril group,the Tangshenkang group showed significantly decreased levels of ACR,24-h UPr,AG and AM（P 〈0.05）.Conclusion： Tangshenkang Granule decreased the urinary protein,attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats,and prevented further injury induced by diabetic nephropathy.展开更多
Objective: To study the effects of Chinese herbal medicine Tangshenkang (TSK) capsule on diabetic nephropathy (DN). Method: Fifty-seven patients with DN were randomly divided into two groups, the TSK-treated group and...Objective: To study the effects of Chinese herbal medicine Tangshenkang (TSK) capsule on diabetic nephropathy (DN). Method: Fifty-seven patients with DN were randomly divided into two groups, the TSK-treated group and the control group. Results: Serious disorders of metabolism showed in DN patients, displayed high levels of thromboxane B2 / 6 - keto- prostaglandin F1a (TXB2 / 6-keto-PGF1a ) ratio and lipid peroxide (LPO) as compared with those of healthy persons. After 6 weeks treatment with TSK, the urinary albumin level reduced obviously (by 51 % ), renal plasma flow (RPF) increased, glomerular filtration rate and LPO levels decreased. A positive correlation was observed between albuminuria level and TXB2/6-keto-PGF1a ratio. The clearance rate of creatinine didn't improve significantly. In the control group, no significant difference in the above-mentioned parameters between pre-treatment and post-treatment was found. Conclusion: TSK capsule possessed a significant effect in improving urinary albumin and glomerular function. These effects might be due to the property of TSK in adjusting TXB2/6-keto-PGF1a ratio and its lipid-peroxidation in DN patients.展开更多
基金Supported by the Scientific Research Project of Colleges and Universities in Anhui Province,China(No.2013z172)the National Natural Science Foundation of China(No.81503590)
文摘Objective： To evaluate the renal protective effect of Tangshenkang Granule（糖肾康颗粒） in a rat model of diabetic nephropathy（DN）.Methods： Forty male Sprague-Dawley rats were randomly divided into control,DN,Tangshenkang and benazepril groups.DN model was established in the rats of DN,Tangshenkang and benazepril groups.Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks,respectively.Urinary albumin and creatinine were detected.The albumin/creatinine（ACR） was calculated in addition to 24 h urinary protein（24-h UPr）,serum creatinine（Scr）,blood urea nitrogen（BUN）,total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL）,and creatinine clearance rate（Ccr）.Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining.The average glomerular diameter（DG）,average glomerular（AG） and mesangial areas（AM） were measured.The thickness of glomerular basement membrane（TGBM） was detected using transmission electron microscope.Results： Compared with rats in the control group,rats in the DN group showed significantly decreased body weight,increased hypertrophy index,24-h urinary volume,24-h UPr,ACR,Scr,BUN,Ccr,blood lipids as well as renal pathological indices including DG,AG,AM,AM/AG and TGBM（P〈0.05）.Compared with the DN group,the weights of rats in the Tangshenkang and benazepril groups were significantly increased,and the renal hypertrophy indices were significantly decreased（P 〈0.05）.The 24-h urinary volumes,ACR,24-h UPr,Scr,BUN,Ccr,LDL,DG,AG,AM and TGBM were obviously decreased（P 〈0.05）.Compared with the benazepril group,the Tangshenkang group showed significantly decreased levels of ACR,24-h UPr,AG and AM（P 〈0.05）.Conclusion： Tangshenkang Granule decreased the urinary protein,attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats,and prevented further injury induced by diabetic nephropathy.
文摘Objective: To study the effects of Chinese herbal medicine Tangshenkang (TSK) capsule on diabetic nephropathy (DN). Method: Fifty-seven patients with DN were randomly divided into two groups, the TSK-treated group and the control group. Results: Serious disorders of metabolism showed in DN patients, displayed high levels of thromboxane B2 / 6 - keto- prostaglandin F1a (TXB2 / 6-keto-PGF1a ) ratio and lipid peroxide (LPO) as compared with those of healthy persons. After 6 weeks treatment with TSK, the urinary albumin level reduced obviously (by 51 % ), renal plasma flow (RPF) increased, glomerular filtration rate and LPO levels decreased. A positive correlation was observed between albuminuria level and TXB2/6-keto-PGF1a ratio. The clearance rate of creatinine didn't improve significantly. In the control group, no significant difference in the above-mentioned parameters between pre-treatment and post-treatment was found. Conclusion: TSK capsule possessed a significant effect in improving urinary albumin and glomerular function. These effects might be due to the property of TSK in adjusting TXB2/6-keto-PGF1a ratio and its lipid-peroxidation in DN patients.
