As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree （Hevea brasiliensis ）, ＂Tapping panel dryness （TPD）＂ causes great yield losses, thereby becoming the most imp...As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree （Hevea brasiliensis ）, ＂Tapping panel dryness （TPD）＂ causes great yield losses, thereby becoming the most important factor limiting rubber production. On the basis of an EST down-regulated in TPD-affected rubber trees, a 903 bp cDNA denoted HbPIP2;2 was isolated from the bark tissue with a combination of in silico cloning and RT-PCR. The cDNA contains an 867 hp ORF, 13 bp 5＇UTR and 23 bp 3＇ UTR. Sequence analysis indicated that HbPIP2;2 encodes 288 amino acids with a theoretical molecular weight （Mw） of 30. 71 kDa and isolectric point （Pi） of 8.20. Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the plasma membrane and harbor one conserved MIP domain that can be grouped into plasma membrane intrinsic proteins （PIPs） of aquaporin （AQP） family. Homolo- gy search revealed that the protein shares a similarity of more than 90% with the homologues in Ricinus communis, Popttlus trichocarpa, Juglans regia and Theobro- ma cacao, suooorting a hie.hly conserved evolution. This study provided basis for further uncovering the regulatory role of AOPs in TPD occurrence.展开更多
[Objective]To address the role of aquaporins（AQPs）in the occurrence of Tapping Panel Dryness（TPD）,a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree（Hevea bra...[Objective]To address the role of aquaporins（AQPs）in the occurrence of Tapping Panel Dryness（TPD）,a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree（Hevea brasiliensis）that causes great losses on rubber production,a tonoplast aquaporin gene associated with TPD occurence was cloned and analyzed.[Method]Based on an EST down-regulated in TPD-affected rubber trees,a 774 bp cDNA designated HbTIP1 was isolated from the bark tissue of Hevea brasiliensis with a combination of in silico cloning and RT-PCR.And the gene structure and sequence characteristics were analyzed using bioinformatics tools.[Result]The cDNA includes a 759 bp ORF,8 bp 5’UTR and 7 bp 3’UTR.Sequence analysis indicated that HbTIP1putatively encodes 252 amino acids with a theoretical molecular weight（Mw）of 25.88 kDa and isolectric point（pI）of 4.96.Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the vacuolar membrane and to harbor one conserved MIP domain that can be grouped into the tonoplast intrinsic proteins（TIPs）of aquaporin family.Homology search revealed that the protein shares a similarity of more than 90%with the homologues in Theobroma cacao,Prunus persica,Citrus sinensis and Ricinus communis,supporting a highly conserved evolution.[Conclusion]This study provided basis for further revealing the regulatory role of AQPs in the TPD occurrence.展开更多
本研究采用A、B和C 3种以壳聚糖为载体的死皮防治药剂开展橡胶树重度死皮(4、5级)的防治试验。结果表明:各处理区死皮长度恢复率依次为:C(55.84%)>A(27.57%)>B(20.11%)>CK(14.97),平均单株胶乳产量依次为:C(45.20 m L)>A(39...本研究采用A、B和C 3种以壳聚糖为载体的死皮防治药剂开展橡胶树重度死皮(4、5级)的防治试验。结果表明:各处理区死皮长度恢复率依次为:C(55.84%)>A(27.57%)>B(20.11%)>CK(14.97),平均单株胶乳产量依次为:C(45.20 m L)>A(39.74 m L)>B(27.50 m L)>CK(18.08 m L),两者变化趋势一致,3种药剂的防效依次为:C(31.91%)>A(16.19%)>B(8.83%)。由此可判断,该3种壳聚糖基死皮防治药剂对重度橡胶树死皮都具有一定的防效,其中C药剂效果最佳,达到显著水平,其次为A药剂和B药剂。本研究提供了一种对橡胶树重度死皮有一定疗效的药剂(C药剂),为壳聚糖在橡胶树死皮防治方面的应用开拓新思路,也为进一步开发更高效的死皮防治药剂提供依据。展开更多
基金Supported by National Natural Science Foundation of China(31371556)Natural Science Foundation of Hainan Province(312026)Fundamental Research Fund for the Rubber Research Institute in Chinese Academy of Tropical Agricultural Sciences(1630022011014)
文摘As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree （Hevea brasiliensis ）, ＂Tapping panel dryness （TPD）＂ causes great yield losses, thereby becoming the most important factor limiting rubber production. On the basis of an EST down-regulated in TPD-affected rubber trees, a 903 bp cDNA denoted HbPIP2;2 was isolated from the bark tissue with a combination of in silico cloning and RT-PCR. The cDNA contains an 867 hp ORF, 13 bp 5＇UTR and 23 bp 3＇ UTR. Sequence analysis indicated that HbPIP2;2 encodes 288 amino acids with a theoretical molecular weight （Mw） of 30. 71 kDa and isolectric point （Pi） of 8.20. Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the plasma membrane and harbor one conserved MIP domain that can be grouped into plasma membrane intrinsic proteins （PIPs） of aquaporin （AQP） family. Homolo- gy search revealed that the protein shares a similarity of more than 90% with the homologues in Ricinus communis, Popttlus trichocarpa, Juglans regia and Theobro- ma cacao, suooorting a hie.hly conserved evolution. This study provided basis for further uncovering the regulatory role of AOPs in TPD occurrence.
基金Supported by National Natural Science Foundation of China(31371556)Natural Science Foundation of Hainan Province(312026)Fundamental Research Fund for the Rubber Research Institute in Chinese Academy of Tropical Agricultural Sciences(1630022011014)
文摘[Objective]To address the role of aquaporins（AQPs）in the occurrence of Tapping Panel Dryness（TPD）,a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree（Hevea brasiliensis）that causes great losses on rubber production,a tonoplast aquaporin gene associated with TPD occurence was cloned and analyzed.[Method]Based on an EST down-regulated in TPD-affected rubber trees,a 774 bp cDNA designated HbTIP1 was isolated from the bark tissue of Hevea brasiliensis with a combination of in silico cloning and RT-PCR.And the gene structure and sequence characteristics were analyzed using bioinformatics tools.[Result]The cDNA includes a 759 bp ORF,8 bp 5’UTR and 7 bp 3’UTR.Sequence analysis indicated that HbTIP1putatively encodes 252 amino acids with a theoretical molecular weight（Mw）of 25.88 kDa and isolectric point（pI）of 4.96.Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the vacuolar membrane and to harbor one conserved MIP domain that can be grouped into the tonoplast intrinsic proteins（TIPs）of aquaporin family.Homology search revealed that the protein shares a similarity of more than 90%with the homologues in Theobroma cacao,Prunus persica,Citrus sinensis and Ricinus communis,supporting a highly conserved evolution.[Conclusion]This study provided basis for further revealing the regulatory role of AQPs in the TPD occurrence.
文摘本研究采用A、B和C 3种以壳聚糖为载体的死皮防治药剂开展橡胶树重度死皮(4、5级)的防治试验。结果表明:各处理区死皮长度恢复率依次为:C(55.84%)>A(27.57%)>B(20.11%)>CK(14.97),平均单株胶乳产量依次为:C(45.20 m L)>A(39.74 m L)>B(27.50 m L)>CK(18.08 m L),两者变化趋势一致,3种药剂的防效依次为:C(31.91%)>A(16.19%)>B(8.83%)。由此可判断,该3种壳聚糖基死皮防治药剂对重度橡胶树死皮都具有一定的防效,其中C药剂效果最佳,达到显著水平,其次为A药剂和B药剂。本研究提供了一种对橡胶树重度死皮有一定疗效的药剂(C药剂),为壳聚糖在橡胶树死皮防治方面的应用开拓新思路,也为进一步开发更高效的死皮防治药剂提供依据。
