Creation of targeted inversion mutations in plants using an RNA-guided endonuclease

Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases(RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were2.6% and 2.2% in the Arabidopsis FLOWERING TIME(At FT) and TERMINAL FLOWER 1(At TFL1)loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.

Automatic target recognition method for inverse synthetic aperture sonar imaging

To address the randomness of target aspect angle and the incompleteness of observed target in inverse synthetic aperture sonar（ISAS） imaging,a method for target recognition is proposed based on topology vector feature（TVF） of multiple highlights. Analysis of the projection relationship from 3 D space to 2 D imaging plane in ISAS indicates that the distance between two highlights in the cross-range scale calibrated image is determined by the distance between the corresponding physical scattering centers. Then, TVFs of different targets, which remain stable in various possibilities of target aspect angle, can be built. K-means clustering technique is used to effectively alleviate effect of the point missing due to incompleteness of the observed target. A nearest neighbor classifier is used to realize the target recognition. The ISAS experimental results using underwater scaled models are provided to demonstrate the effectiveness of the proposed method. A classification rate of 84.0% is reached.

Simultaneous ISAR imaging of group targets flying in formation

This paper proposes a novel inverse synthetic aperture radar（ISAR） imaging method based on second-order keystone transform（KT） and Sandglass transform for group targets flying in a formation with constant accelerated rectilinear motion in the same radar beam. First, range curvature and range walk of each sub-target among group targets are corrected by the second-order KT combined with the quadratic phase term compensation. After range alignment, the signals in each range frequency cell can be modelled as multiple chirp signals and then the Sandglass transform is utilized to cross-range imaging, which transforms the time–frequency distribution of the signals in each range frequency cell into beelines parallel to the slow time axis simultaneously. Finally, cross-range profiles of group targets in each range frequency cell are obtained via a projection of the perk of every scatterer in the two-dimensional accumulation plane onto the frequency axis. The advantage of the proposed method is that it can align range profiles of each sub-target simultaneously and image cross-range profiles directly without separating the returned signals, which simplifies the operation procedure. Simulation results are used to demonstrate the effectiveness of the proposed method.