Relationship between tea pigments and health: A bibliometric and visual analysis

Tea pigments have significant effects on human health.However,more attention have been paid to their physiological functions.The aim of this study was to analyze the quantitative and qualitative impact of tea pigments on human health,together with their current and potential future research directions.The study searched and screened 520 publications on WOS from January 2002 to December 2022.The article collected and collated literature published in the last 20 years and analyzed it bibliometrically for years,journals,countries,authors,topics,keywords and strongest citation bursts.The findings of keywords and strongest citation bursts revealed that the most discussed research topics were anticancer,black tea polyphenol,antioxidant,activator inhibitor,in vivo,gut microbiota,and summarize the relevant literature.As a reference for future research,the literature pointed out current shortcomings and speculated future development trend of tea pigments.

Tea pigments induce cell-cycle arrest and apoptosis in HepG2 cells

AIM： To investigate the molecular mechanisms by which tea pigments exert preventive effects on liver carcinogenesis. METHODS： HepG2 cells were seeded at a density of 5×10^5/well in six-well culture dishes and incubated overnight. The cells then were treated with various concentrations of tea pigments over 3 d, harvested by trypsinization, and counted using a hemocytometer. Flow cytometric analysis was performed by a flow cytometer after propidium iodide labeling. Bd-2 and p21^WAF1 proteins were determined by Western blotting. In addition, DNA laddering assay was performed on treated and untreated cultured HepG2 cells. RESULTS： Tea pigments inhibited the growth of HepG2 cells in a dose-dependent manner. Flow-cytometric analysis showed that tea pigments arrested cell cycle progression at G1 phase. DNA laddering was used to investigate apoptotic cell death, and the result showed that 100 mg/L of tea pigments caused typical DNA laddering. Our study also showed that tea pigments induced upregulation of p21^WAF1 protein and downregulation of Bcl-2 protein. CONCLUSION： Tea pigments induce cell-cycle arrest and apoptosis. Tea pigments may be used as an ideal chemopreventive agent.

Effect of Varieties on Tea Pigment and Chromatic Aberration of Black Tea Infusion

Effects of varieties on the content of tea pigment, L＊, a＊ and b＊ value of chromatic aberration of black tea infusion was studied. The correlation relationship between content of TF, TR, TB and L＊, a＊, b＊ value was extremely significant at P＆lt;0.05. Fresh tea leaves with plucking standard of 1 bud and 2 leaves could man-ufacture black tea with higher content of tea pigment, higher a＊ and b＊ value but lower L＊ value, than those of plucking standard of 1 bud and 3 leaves. But the discrepancy of certain parameter of the two plucking standards changed with vari-eties. Varieties’ quality potential could be concealed by improper plucking standards. Six varieties were divided into four groups on the basis of gross tea polyphenols in fresh tea leaves （GTP）, consumption of tea polyphenols to gross tea polyphenols ratio （CTP/GTP） and gross tea pigment to consumption of tea polyphenols ratio （GP/CTP）. Hybrid performed the very suitability of black tea manufacturing. To en-sure high quality of made black tea, suitable variety should be chosen and reason-able plucking standard also should be taken into consideration.