Quantitative trait loci (QTL) were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatel...Quantitative trait loci (QTL) were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatellite markers were examined on SSC4, SSC6, SSC7, SSC8, and SSC13. The genetic traits included heart weight (HW), lung weight (LW), liver and gallbladder weight (LGW), spleen weight (SPW), stomach weight (STW), small intestine weight (S1W), large intestine weight (LIW), kidney weight (KW), carcass length to the first cervical vertebra (CL1), carcass length to the first thoracic vertebra (CL2), rib numbers (RNS), and teat numbers (TNS). Results indicated that, 3 highly significant QTL (P≤0.01 at chromosome-wise level) for HW (at 30 cM on SSC6), RNS (at 115 cM on SSC7), TNS (at 110 cM on SSC7), and 6 significant QTL (P≤0.05 at chromosome-wise level) for LW (at 119 cM on SSC13), LGW (at 94 cM on SSC6), SPW (at 106 cM on SSC8), SIW (0 cM on SSC4), LIW (170 cM on SSC 4), and TNS (at 95 cM on SSC6) were detected. The phenotypic variances for which these QTL were accounted ranged from 0.04 % to 14.06 %. Most of these QTL had not been previously reported.展开更多
旨在研究VRTN(vertebrae development homolog)基因在巴马香猪群体中的编码区序列特征、组织表达情况、脊椎数性状因果突变位点ins291的等位基因频率及其与乳头数和产仔数性状的关联。本研究采集3头0日龄巴马香猪组织,利用cDNA克隆技术...旨在研究VRTN(vertebrae development homolog)基因在巴马香猪群体中的编码区序列特征、组织表达情况、脊椎数性状因果突变位点ins291的等位基因频率及其与乳头数和产仔数性状的关联。本研究采集3头0日龄巴马香猪组织,利用cDNA克隆技术获得VRTN基因编码区全长序列并进行生物信息学分析,利用荧光定量PCR技术检测VRTN在心、肝、脾、肺、肾、背最长肌和皮下脂肪组织中的表达情况;采集279头经产巴马香猪母猪血液,检测ins291位点在该群体中的频率分布,并与乳头数、产仔数性状进行关联分析。结果表明,巴马香猪VRTN基因编码区全长2 097 bp,其编码的氨基酸序列在不同物种间存在大量保守区域;VRTN基因编码698个氨基酸,预测为亲水性蛋白质,存在1个螺旋转角螺旋域超家族结构功能区、2个低复杂度区域和2个内部重复结构,并与核受体辅抑制子1(NR6A1)、果蝇同源框基因Prospero的脊椎动物同源蛋白2(PROX2)和富亮氨酸重复序列74亚基(LRRC74A)等蛋白具有相互作用;VRTN基因在0日龄巴马香猪各组织中均有表达,其中在背最长肌组织中表达量最高;巴马香猪保种群体中VRTN基因有利突变位点ins291的等位基因频率为21.15%,不同基因型个体之间平均产仔数、总乳头数、左侧乳头数、右侧乳头数和单侧最大乳头数性状均无显著差异(P>0.05),但纯合突变型(ins/ins)个体的乳头数性状均高于其他基因型个体。结果提示,在巴马香猪产业化生产中可通过分子育种手段提高VRTN ins291有利等位基因频率,但不影响其产仔数性状。展开更多
基金This work was supported by the State Key Basic Research and Development Plan of China (No. 2006CB102102)the National Natural Science Foundation of China (No. 30500358).
文摘Quantitative trait loci (QTL) were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatellite markers were examined on SSC4, SSC6, SSC7, SSC8, and SSC13. The genetic traits included heart weight (HW), lung weight (LW), liver and gallbladder weight (LGW), spleen weight (SPW), stomach weight (STW), small intestine weight (S1W), large intestine weight (LIW), kidney weight (KW), carcass length to the first cervical vertebra (CL1), carcass length to the first thoracic vertebra (CL2), rib numbers (RNS), and teat numbers (TNS). Results indicated that, 3 highly significant QTL (P≤0.01 at chromosome-wise level) for HW (at 30 cM on SSC6), RNS (at 115 cM on SSC7), TNS (at 110 cM on SSC7), and 6 significant QTL (P≤0.05 at chromosome-wise level) for LW (at 119 cM on SSC13), LGW (at 94 cM on SSC6), SPW (at 106 cM on SSC8), SIW (0 cM on SSC4), LIW (170 cM on SSC 4), and TNS (at 95 cM on SSC6) were detected. The phenotypic variances for which these QTL were accounted ranged from 0.04 % to 14.06 %. Most of these QTL had not been previously reported.