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Study on Extraction of Oolong Tea Assisted by Ultrasonic Wave and 4C Technique and Its Application
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作者 Guofeng YU Cuishu LENG +9 位作者 Keli FANG Zhiguang LIU Jianhong SUN Zengyu Wang Xuegang LI Shuai Zhang Jian LIU Xulun WEI Qiulin LIU Shoujie NIE 《Agricultural Biotechnology》 2024年第3期24-27,共4页
[Objectives]The extraction conditions of formula oolong tea were investigated by an orthogonal experiment.[Methods]The technical conditions were optimized by the 4C method,and the application of formula oolong tea ext... [Objectives]The extraction conditions of formula oolong tea were investigated by an orthogonal experiment.[Methods]The technical conditions were optimized by the 4C method,and the application of formula oolong tea extract in cigarettes was studied.[Results]①In the experimental range,the best sensory evaluation effect of formula oolong tea extract was obtained with extraction conditions of 70%ethanol as extraction solvent,extraction time h,extraction temperature 25℃,and ultrasonic frequency 80 kHz,and follow-up low-temperature concentration,low-temperature sedimentation and low-temperature centrifugation.②The effects of different centrifugal speeds on the quality of formula oolong tea extract were explored.The formula oolong tea extract obtained under the conditions of 3000 r/min and centrifugal time of 10 min showed the best evaluation effect with soft and delicate smoke,rich smoke fragrance,good comfort and refreshing mouthfeel.③The effective aroma components in the formula oolong tea extract were qualitatively analyzed by GC-MS.[Conclusions]This study provides high-quality raw materials and a theoretical basis for the research of independent flavor blending in cigarette industry enterprises. 展开更多
关键词 Combined application of ultrasonic wave and 4C technique Orthogonal experiment Formula extraction FLAVOR
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The Application of Analytical Techniques to Alpha-Synuclein in Parkinson’s Disease
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作者 Olatayo Adedayo Olahanmi 《American Journal of Analytical Chemistry》 CAS 2024年第9期269-285,共17页
Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by motor symptoms such as tremors, rigidity, and bradykinesia, as well as non-motor symptoms including cognitive impairment and mood ... Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by motor symptoms such as tremors, rigidity, and bradykinesia, as well as non-motor symptoms including cognitive impairment and mood disorders. A hallmark of PD is the accumulation of alpha-synuclein, a presynaptic neuronal protein that aggregates to form Lewy bodies, leading to neuronal dysfunction and cell death. The study of alpha-synuclein and its pathological forms is crucial for understanding the etiology of PD and developing effective diagnostic and therapeutic strategies. Analytical techniques play a pivotal role in elucidating the structure, function, and aggregation mechanisms of alpha-synuclein. Biochemical methods such as Western blotting and enzyme-linked immunosorbent assay (ELISA) are employed to detect and quantify alpha-synuclein in biological samples, offering insights into its expression levels and post-translational modifications. Imaging techniques like immunohistochemistry and positron emission tomography (PET) allow for the visualization of alpha-synuclein aggregates in tissue samples and in vivo, respectively, facilitating the study of its spatial distribution and progression in PD Spectroscopic methods, including nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry, provide detailed structural information on alpha-synuclein and its isoforms, aiding in the identification of conformational changes associated with aggregation. Emerging techniques such as cryo-electron microscopy (Cryo-EM) and single-molecule fluorescence enable high-resolution structural analysis and real-time monitoring of alpha-synuclein aggregation dynamics, respectively. The application of these analytical techniques has significantly advanced our understanding of the pathophysiological role of alpha-synuclein in PD. They have contributed to the identification of potential biomarkers for early diagnosis and the evaluation of therapeutic interventions targeting alpha-synuclein aggregation. Despite technical limitations and challenges in clinical translation, ongoing advancements in analytical methodologies hold promise for improving the diagnosis, monitoring, and treatment of Parkinson’s disease through a deeper understanding of alpha-synuclein pathology. 展开更多
关键词 Parkinson’s Disease ALPHA-SYNUCLEIN techniqueS
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Electrospinning-hot pressing technique for the fabrication of thermal and electrical storage membranes and its applications
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作者 Panpan Che Baoshan Xie +7 位作者 Penghui Cao Youfu Lv Daifei Liu Huali Zhu Xianwen Wu Zhangxing He Jian Chen Chuanchang Li 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第9期1945-1964,共20页
The combination of electrospinning and hot pressing,namely the electrospinning-hot pressing technique(EHPT),is an efficient and convenient method for preparing nanofibrous composite materials with good energy storage ... The combination of electrospinning and hot pressing,namely the electrospinning-hot pressing technique(EHPT),is an efficient and convenient method for preparing nanofibrous composite materials with good energy storage performance.The emerging composite membrane prepared by EHPT,which exhibits the advantages of large surface area,controllable morphology,and compact structure,has attracted immense attention.In this paper,the conduction mechanism of composite membranes in thermal and electrical energy storage and the performance enhancement method based on the fabrication process of EHPT are systematically discussed.Moreover,the state-of-the-art applications of composite membranes in these two fields are introduced.In particular,in the field of thermal energy storage,EHPT-prepared membranes have longitudinal and transverse nanofibers,which generate unique thermal conductivity pathways;also,these nanofibers offer enough space for the filling of functional materials.Moreover,EHPT-prepared membranes are beneficial in thermal management systems,building energy conservation,and electrical energy storage,e.g.,improving the electrochemical properties of the separators as well as their mechanical and thermal stability.The application of electrospinning-hot pressing membranes on capacitors,lithium-ion batteries(LIBs),fuel cells,sodium-ion batteries(SIBs),and hydrogen bromine flow batteries(HBFBs)still requires examination.In the future,EHPT is expected to make the field more exciting through its own technological breakthroughs or be combined with other technologies to produce intelligent materials. 展开更多
关键词 electrospinning-hot pressing technique thermal storage electrical storage composite membranes NANOFIBER
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Recent Advance in Synaptic Plasticity Modulation Techniques for Neuromorphic Applications
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作者 Yilin Sun Huaipeng Wang Dan Xie 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第10期403-434,共32页
Manipulating the expression of synaptic plasticity of neuromorphic devices provides fascinating opportunities to develop hardware platforms for artifi-cial intelligence.However,great efforts have been devoted to explo... Manipulating the expression of synaptic plasticity of neuromorphic devices provides fascinating opportunities to develop hardware platforms for artifi-cial intelligence.However,great efforts have been devoted to exploring biomimetic mechanisms of plasticity simulation in the last few years.Recent progress in various plasticity modulation techniques has pushed the research of synaptic electronics from static plasticity simulation to dynamic plasticity modulation,improving the accuracy of neuromorphic computing and providing strategies for implementing neuromorphic sensing functions.Herein,several fascinating strategies for synap-tic plasticity modulation through chemical techniques,device structure design,and physical signal sensing are reviewed.For chemical techniques,the underly-ing mechanisms for the modification of functional materials were clarified and its effect on the expression of synaptic plasticity was also highlighted.Based on device structure design,the reconfigurable operation of neuromorphic devices was well demonstrated to achieve programmable neuromorphic functions.Besides,integrating the sensory units with neuromorphic processing circuits paved a new way to achieve human-like intelligent perception under the modulation of physical signals such as light,strain,and temperature.Finally,considering that the relevant technology is still in the basic exploration stage,some prospects or development suggestions are put forward to promote the development of neuromorphic devices. 展开更多
关键词 Plasticity modulation Dynamic plasticity Chemical techniques Programmable operation Neuromorphic sensing
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Advances in the application of auxiliary imaging techniques in parathyroid diseases
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作者 Lei Lu Hong-Qing Shang 《World Journal of Clinical Cases》 SCIE 2024年第17期2946-2950,共5页
Hypoparathyroidism is one of the main complications after total thyroidectomy,severely affecting patients’quality of life.How to effectively protect parathyroid function after surgery and reduce the incidence of hypo... Hypoparathyroidism is one of the main complications after total thyroidectomy,severely affecting patients’quality of life.How to effectively protect parathyroid function after surgery and reduce the incidence of hypoparathyroidism has always been a key research area in thyroid surgery.Therefore,precise localization of parathyroid glands during surgery,effective imaging,and accurate surgical resection have become hot topics of concern for thyroid surgeons.In response to this clinical phenomenon,this study compared several different imaging methods for parathyroid surgery,including nanocarbon,indocyanine green,near-infrared imaging techniques,and technetium-99m methoxyisobutylisonitrile combined with gamma probe imaging technology.The advantages and disadvantages of each method were analyzed,providing scientific recommendations for future parathyroid imaging.In recent years,some related basic and clinical research has also been conducted in thyroid surgery.This article reviewed relevant literature and provided an overview of the practical application progress of various imaging techniques in parathyroid surgery. 展开更多
关键词 Imaging technique Parathyroid gland Thyroid surgery HYPOPARATHYROIDISM COMPLICATION
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Application of oblique photogrammetry technique in geological hazard identification and decision management
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作者 Min Tang Xi Mei +3 位作者 Yanna Li Chen Chen Xiuju Liu Heng Lu 《Earthquake Research Advances》 CSCD 2024年第3期34-41,共8页
With the continuous development of the oblique photography technique, it has been used more and more widely in the field of geological disasters. It can quickly obtain the three-dimensional(3D) real scene model of dan... With the continuous development of the oblique photography technique, it has been used more and more widely in the field of geological disasters. It can quickly obtain the three-dimensional(3D) real scene model of dangerous mountainous areas under the premise of ensuring the safety of personnel while restoring the real geographic information as much as possible. However, geological disaster areas are often accompanied by many adverse factors such as cliffs and dense vegetation. Based on this, the paper introduced the flight line design of oblique photogrammetry, analyzed the multi-platform data fusion processing, studied the multi-period data dynamic evaluation technology and proposed the application methods of data acquisition, early warning, disaster assessment and decision management suitable for geological disaster identification through the analysis of actual cases, which will help geologists to plan and control geological work more scientifically and rationally, improve work efficiency and reduce the potential personnel safety hazards in the process of geological survey, to offer technical support to the application of oblique photogrammetry in geological disaster identification and decision making and provide the scientific basis for personal and property safety protection and later-stage geological disaster management in disaster areas. 展开更多
关键词 Oblique photography technique Three-dimensional models Geological hazards Data integration Disaster assessment Decision management
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Establishment and Application of Prevention and Control Techniques of New Sugarcane White Leaf Disease
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作者 Xiaoyan WANG Ruonan QIU +8 位作者 Hong XU Yulong JIANG Rongyue ZHANG Fengyun ZHANG Ming GONG Jinxue LEI Hongli SHAN Jie LI Yingkun HUANG 《Agricultural Biotechnology》 2024年第4期18-20,27,共4页
Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10... Aiming at the basic and key technical problems in prevention and control of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and control of new SCWL after 10 years of collaborative research,and achieved several innovative achievements.SCWL phytoplasmas newly recorded in China and the new subgroup of SCWL phytoplasmas(16SrXI-D)were discovered for the first time in Yunnan,and the whole genome analysis of the epidemic subgroup was completed.The main transmission source of SCWL pathogens has been identified as infected seed canes,and Tettigoniella viridis and Clovia conifer were newly discovered as vectors for virus transmission.The disease resistance of 25 main varieties was identified,and 10 control varieties were selected.The prevention and control strategy of"emphasizing early warning,strictly carrying out quarantine,blocking the vectors and controlling residual plants"was put forward,and a comprehensive prevention technique was established through integration of various techniques,and standardized technical regulations were formulated for demonstration application.The promotion and application of these achievements have realized scientific prevention and control of SCWL,effectively curbed the spread of SCWL,and ensured the safety of sugarcane producing areas in China,achieving great economic,social and ecological benefits and providing technical support for high-quality development,loss reduction and efficiency improvement of China's sugar industry. 展开更多
关键词 Sugarcane white leaf disease Comprehensive prevention and control application
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Research on the Application of Contemporary Wireless Enamel Technique in the Teaching of Traditional Jewelry Craft Course for College Student
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作者 Zhengxu Yan Xiao Lu +1 位作者 Toshie Miyakawa Rui Bao 《宝石和宝石学杂志(中英文)》 CAS 2024年第4期86-93,共8页
Taking the wireless enamel(wireless enamel refers to make enamel works without filigree)production technique in jewelry practice course as the research object,combined with years of enamel teaching experience in the G... Taking the wireless enamel(wireless enamel refers to make enamel works without filigree)production technique in jewelry practice course as the research object,combined with years of enamel teaching experience in the Gemmological Institute of China University of Geosciences(Wuhan),this study expounds the theoretical class,design preparation,creative idea and technique practice application from the course teaching objectives and the implementation,which also emphasizes the practical improvability of students’design practice ability and innovative thinking ability.During the period,it focuses on the core processes,such as wireless enamel glaze method,object correction,firing time,composite application and system integration,to help students solve the key and difficult problems in the wireless enamel firing process. 展开更多
关键词 wireless enamel technique practical teaching
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Masquelet technique using an allogeneic cortical bone graft for a large bone defect:A case report
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作者 Hai-Yang Zong Yu Liu +2 位作者 Xing Yin Wei Zhou Nan Li 《World Journal of Clinical Cases》 SCIE 2025年第5期36-43,共8页
BACKGROUND The induced-membrane technique was initially described by Masquelet as an effective treatment for large bone defects,especially those caused by infection.Here,we report a case of chronic osteomyelitis of th... BACKGROUND The induced-membrane technique was initially described by Masquelet as an effective treatment for large bone defects,especially those caused by infection.Here,we report a case of chronic osteomyelitis of the radius associated with a 9 cm bone defect,which was filled with a large allogeneic cortical bone graft from a bone bank.Complete bony union was achieved after 14 months of follow-up.Previous studies have used autogenous bone as the primary bone source for the Masquelet technique;in our case,the exclusive use of allografts is as successful as the use of autologous bone grafts.With the advent of bone banks,it is possible to obtain an unlimited amount of allograft,and the Masquelet technique may be further improved based on this new way of bone grafting.CASE SUMMARY In this study,we reported a case of repair of a long bone defect in a 40-year-old male patient,which was characterized by the utilization of allograft cortical bone combined with the Masquelet technique for the treatment of the patient's long bone defect in the forearm.The patient's results of functional recovery of the forearm were surprising,which further deepens the scope of application of Masquelet technique and helps to strengthen the efficacy of Masquelet technique in the treatment of long bones indeed.CONCLUSION Allograft cortical bone combined with the Masquelet technique provides a new method of treatment to large bone defect. 展开更多
关键词 OSTEOMYELITIS Bone defect Allogeneic cortical bone Masquelet technique Membrane induction technique Case report
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Application of an electrophysiological technique combined with auricular acupuncture in gynecological perioperative gastrointestinal dysfunction
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作者 Mei-Hua Wu Yan Ke +5 位作者 Xiao-Lei Song Nan Jiang Na Yu Wei-Wei Sun Mei Wang Wen-Ming Cao 《Clinical Research Communications》 2024年第4期21-28,共8页
Objective:This study aimed to evaluate the effectiveness and safety of combining low-frequency pulsed electrophysiological techniques with auricular acupuncture(a fusion of traditional Chinese and Western medicine)for... Objective:This study aimed to evaluate the effectiveness and safety of combining low-frequency pulsed electrophysiological techniques with auricular acupuncture(a fusion of traditional Chinese and Western medicine)for patients experiencing perioperative gastrointestinal dysfunction(POGD)following gynecological surgery.Methods:Eighty patients undergoing gynecological surgery were randomly assigned to an experimental group and a control group.Both groups received enhanced recovery after surgery(ERAS)care,with the experimental group receiving additional treatment combining electrophysiology and auricular acupuncture.Postoperative recovery indicators,including bowel sound recovery time,time to first exhaust and defecation,duration and incidence of abdominal pain and distension,nausea and vomiting,and specific gastrointestinal markers(HSP70,I-FABP,DAO,D-lactate),were monitored and compared between the two groups.Results:After treatment,the postoperative bowel sound recovery time,first postoperative exhaust time,duration of abdominal pain,and duration of abdominal distension were shorter in the experimental group than in the control group(P<0.05).The 6-hour and 48-hour integral values of the experimental group were significantly lower than those of the control group.In the experimental group,60%of the patients were completely cured of gastrointestinal function after surgery,whereas only 32.5%of the patients in the control group were cured.The main symptom scores(abdominal pain,abdominal distension,nausea and vomiting)on the third postoperative day and the total symptom scores on the third postoperative day were significantly lower in the experimental group than in the control group(P<0.05).The values of HSP70,I-FABP,DAO,and D-lactate in the experimental group on postoperative day one were significantly lower than those in the control group(P<0.05).Conclusion:The combination of the Chinese and Western medicine ERAS programs of low-frequency pulse electrophysiology technology plus auricular acupuncture can accelerate local gastrointestinal blood circulation during the perioperative period of gynecology,promote the recovery of gastrointestinal function,promote anal exhaustion,and prevent the occurrence of postoperative abdominal pain and bloating.The combination of Chinese and Western medicine ERAS can increase gastrointestinal fluid secretion,increase gastrointestinal hormone and blood flow values,increase gastrointestinal electrophysiological detection values,and change the indicators of gastrointestinal dysfunction. 展开更多
关键词 electrophysiological technique auricular acupuncture ERAS gynecological surgery POGD
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Application of nanotechnology to dentistry:Impact of graphene nanocomposites on clinical air quality
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作者 Ruth Rodríguez Montaño Mario A Alarcón-Sánchez +2 位作者 Melissa Martínez Nieto Juan J Varela Hernández Sarah M LomelíMartínez 《World Journal of Clinical Cases》 SCIE 2025年第8期1-7,共7页
Concerns about air quality in dental clinics where aerosol generation during procedures poses significant health risks,have prompted investigations on advanced disinfection technologies.This editorial describes the st... Concerns about air quality in dental clinics where aerosol generation during procedures poses significant health risks,have prompted investigations on advanced disinfection technologies.This editorial describes the strengths and limitations of ventilation and aerosol control measures in dental offices,especially with respect to the use of graphene nanocomposites.The potential of graphene nanocomposites as an innovative solution to aerosol-associated health risks is examined in this review due to the unique properties of graphene(e.g.,high con-ductivity,mechanical strength,and antimicrobial activity).These properties have produced promising results in various fields,but the application of graphene in dentistry remains unexplored.The recent study by Ju et al which was published in World Journal of Clinical Cases evaluated the effectiveness of graphene-based air disinfection systems in dental clinics.The study demonstrated that graphene-based disinfection techniques produced significant reductions in suspended particulate matter and bacterial colony counts,when co-mpared with traditional methods.Despite these positive results,challenges such as material saturation,frequency of filter replacement,and associated costs must be addressed before widespread adoption of graphene-based disinfection techniques in clinical practice.Therefore,there is need for further research on material structure optimization,long-term safety evaluations,and broader clinical applications,in order to maximize their positive impact on public health. 展开更多
关键词 GRAPHENE NANOCOMPOSITES Antibacterial activity Biomedical applications Air disinfection
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An Enhanced Lung Cancer Detection Approach Using Dual-Model Deep Learning Technique
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作者 Sumaia Mohamed Elhassan Saad Mohamed Darwish Saleh Mesbah Elkaffas 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期835-867,共33页
Lung cancer continues to be a leading cause of cancer-related deaths worldwide,emphasizing the critical need for improved diagnostic techniques.Early detection of lung tumors significantly increases the chances of suc... Lung cancer continues to be a leading cause of cancer-related deaths worldwide,emphasizing the critical need for improved diagnostic techniques.Early detection of lung tumors significantly increases the chances of successful treatment and survival.However,current diagnostic methods often fail to detect tumors at an early stage or to accurately pinpoint their location within the lung tissue.Single-model deep learning technologies for lung cancer detection,while beneficial,cannot capture the full range of features present in medical imaging data,leading to incomplete or inaccurate detection.Furthermore,it may not be robust enough to handle the wide variability in medical images due to different imaging conditions,patient anatomy,and tumor characteristics.To overcome these disadvantages,dual-model or multi-model approaches can be employed.This research focuses on enhancing the detection of lung cancer by utilizing a combination of two learning models:a Convolutional Neural Network(CNN)for categorization and the You Only Look Once(YOLOv8)architecture for real-time identification and pinpointing of tumors.CNNs automatically learn to extract hierarchical features from raw image data,capturing patterns such as edges,textures,and complex structures that are crucial for identifying lung cancer.YOLOv8 incorporates multiscale feature extraction,enabling the detection of tumors of varying sizes and scales within a single image.This is particularly beneficial for identifying small or irregularly shaped tumors that may be challenging to detect.Furthermore,through the utilization of cutting-edge data augmentation methods,such as Deep Convolutional Generative Adversarial Networks(DCGAN),the suggested approach can handle the issue of limited data and boost the models’ability to learn from diverse and comprehensive datasets.The combined method not only improved accuracy and localization but also ensured efficient real-time processing,which is crucial for practical clinical applications.The CNN achieved an accuracy of 97.67%in classifying lung tissues into healthy and cancerous categories.The YOLOv8 model achieved an Intersection over Union(IoU)score of 0.85 for tumor localization,reflecting high precision in detecting and marking tumor boundaries within the images.Finally,the incorporation of synthetic images generated by DCGAN led to a 10%improvement in both the CNN classification accuracy and YOLOv8 detection performance. 展开更多
关键词 Lung cancer detection dual-model deep learning technique data augmentation CNN YOLOv8
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Advancements and challenges in neuroimaging for the diagnosis of intracranial aneurysms:Addressing false positive diagnoses and emerging techniques
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作者 Nanthida Arora Sombat Muengtaweepongsa 《World Journal of Clinical Cases》 SCIE 2025年第6期48-50,共3页
Despite advancements in neuroimaging,false positive diagnoses of intracranial aneurysms remain a significant concern.This article examines the causes,prevalence,and implications of such false-positive diagnoses.We dis... Despite advancements in neuroimaging,false positive diagnoses of intracranial aneurysms remain a significant concern.This article examines the causes,prevalence,and implications of such false-positive diagnoses.We discuss how conditions like arterial occlusion with vascular stump formation and infundibular widening can mimic aneurysms,particularly in the anterior circulation.The article compares various imaging modalities,including computer tomography angiogram,magnetic resonance imaging/angiography,and digital subtraction angiogram,highlighting their strengths and limitations.We emphasize the im-portance of accurate differentiation to avoid unnecessary surgical interventions.The potential of emerging technologies,such as high-resolution vessel wall ima-ging and deep neural networks for automated detection,is explored as promising avenues for improving diagnostic accuracy.This manuscript underscores the need for continued research and clinical vigilance in the diagnosis of intracranial aneurysms. 展开更多
关键词 Intracranial aneurysms Neuroimaging techniques Computed tomographic angiography Magnetic resonance angiography Digital subtraction angiography False positive diagnoses
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APPROXIMATION TECHNIQUES FOR APPLICATION OF GENETIC ALGORITHMS TO STRUCTURAL OPTIMIZATION 被引量:1
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作者 金海波 丁运亮 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2003年第2期147-154,共8页
Although the genetic algorithm (GA) has very powerful robustness and fitness, it needs a large size of population and a large number of iterations to reach the optimum result. Especially when GA is used in complex str... Although the genetic algorithm (GA) has very powerful robustness and fitness, it needs a large size of population and a large number of iterations to reach the optimum result. Especially when GA is used in complex structural optimization problems, if the structural reanalysis technique is not adopted, the more the number of finite element analysis (FEA) is, the more the consuming time is. In the conventional structural optimization the number of FEA can be reduced by the structural reanalysis technique based on the approximation techniques and sensitivity analysis. With these techniques, this paper provides a new approximation model-segment approximation model, adopted for the GA application. This segment approximation model can decrease the number of FEA and increase the convergence rate of GA. So it can apparently decrease the computation time of GA. Two examples demonstrate the availability of the new segment approximation model. 展开更多
关键词 approximation techniques segment approximation model genetic algorithms structural optimization sensitivity analysis
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Application of Filming Technique in the DUS Test for PVP Information
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作者 高玲 徐丽 +1 位作者 刘迪发 张如莲 《Agricultural Science & Technology》 CAS 2013年第6期835-837,共3页
As the great progress has appeared in the field of protection of new varieties of plants, the standardization of the DUS (Distinctness, Uniformity, Stability) test procedure has become more important. However, the s... As the great progress has appeared in the field of protection of new varieties of plants, the standardization of the DUS (Distinctness, Uniformity, Stability) test procedure has become more important. However, the specification of filming technique plays an important role in the DUS test of new varieties of plants. In this paper, we analyzed the status quo and significance of the application of filming technique in the PVP (Plant Variety Protection) system, and provided an introduction about the application of filming technique in DUS test in China. 展开更多
关键词 Filming technique DUS test application
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Real-Time PCR Technique and Its Application in Quantification of Plant Nucleic Acid Molecules 被引量:8
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作者 刘进元 《Acta Botanica Sinica》 CSCD 2003年第6期631-637,共7页
Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of ini... Real-time PCR is a closed DNA amplification system that skillfully integrates biochemical, photoelectric and computer techniques. Fluorescence data acquired once per cycle provides rapid absolute quantification of initial template copy numbers as PCR products are generated. This technique significantly simplifies and accelerates the process of producing reproducible quantification of nucleic acid molecules. It not only is a sensitive, accurate and rapid quantitative method, but it also provides an easier way to calculate the absolute starting copy number of nucleic acid molecules to be tested. Together with molecular bio-techniques, like microarray, real-time PCR will play a very important role in many aspects of molecular life science such as functional gene analysis and disease molecular diagnostics. This review introduces the detailed principles and application of the real-time PCR technique, describes a recently developed system for exact quantification of AUX/IAA genes In Arabidopsis, and discusses the problems with the real-time PCR process. 展开更多
关键词 real-time PCR technique quantification of plant nucleic acid molecules gene expression molecular medicine
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Application and Promotion of Non-pesticide Replacing High-toxic Pesticide Techniques and Its Benefit Analysis in Kunming City
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作者 窦秦川 杨珺 +1 位作者 罗嵘 傅杨 《Plant Diseases and Pests》 CAS 2011年第2期51-57,共7页
[ Objective] The paper was to operate the application and promotion of non-pesticide replacing high-toxic pesticides techniques in Kunming City, and to analyze its benefit. [ Method ] Through application and promotion... [ Objective] The paper was to operate the application and promotion of non-pesticide replacing high-toxic pesticides techniques in Kunming City, and to analyze its benefit. [ Method ] Through application and promotion of frequency trembler grid lamps, sticking plate trapping technology, construction of treatment ponds for field waste vegetable leaves, standardized (accurate) cultivation techniques, cultivation techniques of disease-resistant varieties and diverse cultivation technologies, the promotion benefit of non-pesticide replacing high-toxic pesticides techniques was comprehensively investigated and evaluated. [ Result ] The appli- cation and promotion area of non-pesticide replacing high-toxic pesticides techniques in Kunming City during 2006 -2010 reached 94 667 hm2. The investigation on control efforts and quantitative analysis of cost/benefit showed that the beneficial result of application and promotion of non-pesticide replacing high-toxic pesticides techniques was higher than the direct benefit of application and promotion of traditional pesticide replacing techniques. This improvement innovated the traditional pesticide replacing method in replacement work of high-toxic pesticides, reducing the usage volume of pesticide in Kunming City. [ Conclusion] The application and promotion of non-pesticide replacing high-toxic pesticides techniques improved the economic, social and ecological benefit of replacement work of high-toxic pesticides, protected the agricultural ecological environment and promoted the sustainable development of agricultural production. 展开更多
关键词 Non-pesticide replacing high-toxic pesticides techniques application and promotion Benefit analysis
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The Application of Modern Art Techniques in T.S. Eliot's The Waste Land
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作者 卢云 李雪芹 《英语广场(学术研究)》 2011年第Z5期14-15,18,共3页
The literature in 20th century is mainly characterized by irrationalism presented in modernism. Under the background, this paper aims to explore how Eliot attempts to use modern art techniques in The Waste Land to rev... The literature in 20th century is mainly characterized by irrationalism presented in modernism. Under the background, this paper aims to explore how Eliot attempts to use modern art techniques in The Waste Land to reveal a real life to readers. 展开更多
关键词 the application MODERN ART technique T.S. Eliot’s The WASTE LAND
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Grafting Techniques of Sophora japonica f. flavi-rameus and Its Application in Gardens
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作者 韩亚利 《Journal of Landscape Research》 2011年第3期29-31,55,共4页
The method of cultivating seedlings of Sophora japonica f.flavi-rameus on rootstocks of Sophora japonica Linn.was introduced in this study,including rootstock cultivation,grafting and post-grafting management,specific... The method of cultivating seedlings of Sophora japonica f.flavi-rameus on rootstocks of Sophora japonica Linn.was introduced in this study,including rootstock cultivation,grafting and post-grafting management,specifically,seed collection,sowing,and breeding large seedlings in the cultivation of rootstocks;grafting time,scion collection and treatment,rootstock treatment,as well as techniques of stump grafting and bud grafting;focuses of post-grafting management,such as checking the survival conditions and untying the film,bud picking,pinching,topiary work,moisture and fertilization management,disease and pest control.Then the application of Sophora japonica f.flavi-rameus in gardens was analyzed,and it was proposed that biological characteristics and aesthetic principles should be followed,proper planting patterns should be applied,such as isolated planting,group planting,mass planting,linear planting and so on,moreover,it could also be combined with other species to create outstanding landscape effects. 展开更多
关键词 SOPHORA japonica f.flavi-rameus SCION GRAFTING Bud GRAFTING GARDENING application
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ODE CONVERSION TECHNIQUES AND THEIR APPLICATIONS IN COMPUTATIONAL MECHANICS 被引量:14
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作者 袁驷 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 1991年第3期283-288,共6页
In this paper,a number of ordinary differential equation(ODE)conversion techniques for trans- formation of nonstandard ODE boundary value problems into standard forms are summarised,together with their applications to... In this paper,a number of ordinary differential equation(ODE)conversion techniques for trans- formation of nonstandard ODE boundary value problems into standard forms are summarised,together with their applications to a variety of boundary value problems in computational solid mechanics,such as eigenvalue problem,geometrical and material nonlinear problem,elastic contact problem and optimal design problems through some simple and representative examples,The advantage of such approach is that various ODE bounda- ry value problems in computational mechanics can be solved effectively in a unified manner by invoking a stand- ard ODE solver. 展开更多
关键词 ODE SOLVERS conversion techniques computational mechanics
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