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Meiotic Behavior of 1BL/1RS Translocation Chromosome and Alien Chromosome in Two Tri-genera Hybrids 被引量:3
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作者 李义文 李振声 贾旭 《Acta Botanica Sinica》 CSCD 2002年第7期821-826,共6页
The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivisio... The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background. 展开更多
关键词 MEIOSIS chromatin univalent 1BL/1RS translocation chromosome fluorescence in situ hybridization
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LncRNA PVT1对弥漫大B细胞淋巴瘤细胞活性的影响及其机制
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作者 路晓辉 李文永 +1 位作者 王孟林 陈香莉 《青岛大学学报(医学版)》 CAS 2024年第3期381-387,共7页
目的 探究长链非编码RNA(LncRNA)浆细胞瘤变体异位基因1(PVT1)对弥漫大B细胞淋巴瘤(DLBCL)细胞生物学行为的影响,并分析其潜在机制。方法 收集41例DLBCL病人和15例淋巴结反应性增生(RLH)病人的组织标本,体外培养人正常B淋巴细胞GM12878... 目的 探究长链非编码RNA(LncRNA)浆细胞瘤变体异位基因1(PVT1)对弥漫大B细胞淋巴瘤(DLBCL)细胞生物学行为的影响,并分析其潜在机制。方法 收集41例DLBCL病人和15例淋巴结反应性增生(RLH)病人的组织标本,体外培养人正常B淋巴细胞GM12878和人DLBCL细胞(OCI-Ly3、U2932、TMD8),对TMD8细胞进行转染,将其分为control组(只转染Lipofectamine-2000)、si-NC组(转染si-NC)、inhibitor-NC组(转染inhibitor-NC)、si-PVT1组(转染si-PVT1)、miR-145-5p inhibitor组(转染miR-145-5p inhibitor)、si-PVT1+miR-145-5p inhibitor组(转染si-PVT1和miR-145-5p inhibitor)。应用qRT-PCR方法检测各组细胞PVT1 mRNA和miR-145-5p表达,Western Blot方法检测CDK6蛋白表达,CCK-8法检测TMD8细胞增殖,流式细胞术检测TMD8细胞周期变化,Transwell实验检测TMD8细胞迁移和侵袭能力,RNA pull down和双荧光素酶报告基因法验证PVT1、miR-145-5p与细胞周期蛋白依赖性激酶6(CDK6)的靶向关系。结果 DLBCL组织PVT1 mRNA、CDK6蛋白的表达水平高于RLH组织,miR-145-5p表达低于RLH组织(t=14.264~24.445,P<0.05)。与GM12878细胞比较,OCI-Ly3、U2932、TMD8细胞中PVT1 mRNA、CDK6蛋白表达均增加,miR-145-5p表达均减少(F=69.557~234.718,P<0.05)。6组细胞PVT1 mRNA、miR-145-5p、CDK6蛋白表达及增殖率、G0/G1期细胞比例、S期细胞比例、迁移和侵袭细胞数差异有统计学意义(F=25.589~319.150,P<0.05);与control组比较,si-PVT1组细胞PVT1 mRNA、CDK6蛋白、增殖率、S期细胞比例、迁移和侵袭数量降低,miR-145-5p表达、G0/G1期细胞比例升高(P<0.05),miR-145-5p inhibitor组呈相反变化(P<0.05);下调miR-145-5p表达可减弱敲低PVT1对TMD8细胞恶性生物学行为的抑制作用(P<0.05)。过表达PVT1 mRNA增高CDK6蛋白表达、细胞增殖率、S期细胞比例、迁移和侵袭数量,降低miR-145-5p表达、G0/G1期的细胞比例(F=38.025~327.887,P<0.05)。miR-145-5p是PVT1的靶基因,且miR-145-5p可靶向下调CDK6表达。结论 敲低PVT1可抑制DLBCL细胞恶性生物学行为,其作用机制可能与调控miR-145-5p/CDK6轴有关。 展开更多
关键词 淋巴瘤 大B细胞 弥漫性 RNA 长链非编码 浆细胞瘤变体异位基因1 miR-145-5p 细胞周期蛋白依赖激酶6
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缺血性疾病患者血清lncRNA PVT1和FOXM1表达水平及联合心脏磁共振延迟强化成像与预后的关系
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作者 尹晓翔 赵森 +2 位作者 郭颖 刘梦雯 庄琰 《中国CT和MRI杂志》 2024年第3期73-75,共3页
目的 探讨缺血性疾病患者血清长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)和叉头框转录因子M1(FOX M1)表达水平及联合心脏钆对比剂延迟增强磁共振成像(LGE-MRI)与预后的关系。方法 选取2021年2月-2022年2月我院收治的缺血性心脏... 目的 探讨缺血性疾病患者血清长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)和叉头框转录因子M1(FOX M1)表达水平及联合心脏钆对比剂延迟增强磁共振成像(LGE-MRI)与预后的关系。方法 选取2021年2月-2022年2月我院收治的缺血性心脏病患者118例即为研究组,随访一年根据随访过程中是否发生主要心脏不良事件(MACE),分为MACE组32例,无MACE组86例。同期选择在我院体检健康的志愿者118例为对照组。实时荧光定量PCR(qRT-PCR)检测血清lncRNA PVT1的相对表达量。采用酶联免疫吸附试验(E LISA)检测FOXM1水平。受试者工作特征(ROC)曲线分析LGE-MRI、血清lncRNA PVT1和FOXM1对缺血性心脏病患者预后发生MACE的预测价值。结果 研究组患者DBP、SBP、TG、TC、 LDL-C、GLU水平与对照组相比显著升高,HDL-C水平显著降低(P<0.05)。与对照组相比,研究组的血清中lncRNA PVT1和FOXM1水平显著升高(P<0.05)。与无MACE组相比,MACE组患者DBP、SBP、TC、LDL-C水平显著升高, HDL-C水平显著降低(P<0.05)。与无MACE组相比,MACE组患者血清中lncRNA PVT1和FOXM1水平显著升高(P<0.05)。MACE组的LGE-MRI阳性数量显著高于无MACE组(P<0.)05。与LGE-MRI、血清lncRNA PVT1和FOXM1单独预测相比,三者联合预测MACE发生的AUC更高(Z=7.221,P<0.001;Z=7.737,P<0.001;Z=7.091,P<0.001)。结论 缺血性心脏病预后发生MACE的患者血清lncRNA PVT1和FOXM1水平呈高表达,二者联合LGE-MRI对MACE的发生有一定的预测价值。 展开更多
关键词 长链非编码RNA浆细胞瘤转化迁移基因1 叉头框转录因子M1 心脏钆对比剂延迟增强磁共振成像 预后 缺血性疾病
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血清lncRNA ANRIL、lncRNA PVT1水平与急性呼吸窘迫综合征患儿病情及预后的关系
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作者 杨静 刘华朋 +1 位作者 柳旎 朱萍 《实用临床医药杂志》 CAS 2024年第14期77-81,86,共6页
目的探究血清长链非编码RNA INK4位点反义非编码RNA(lncRNA ANRIL)、长链非编码RNA浆细胞瘤变异易位基因1(lncRNA PVT1)水平与急性呼吸窘迫综合征(ARDS)患儿病情及预后的关系。方法选取124例确诊的ARDS患儿为患病组,另选取124例同期体... 目的探究血清长链非编码RNA INK4位点反义非编码RNA(lncRNA ANRIL)、长链非编码RNA浆细胞瘤变异易位基因1(lncRNA PVT1)水平与急性呼吸窘迫综合征(ARDS)患儿病情及预后的关系。方法选取124例确诊的ARDS患儿为患病组,另选取124例同期体检健康者为对照组。ARDS患儿根据病情严重程度分为重度组(34例)、中度组(42例)和轻度组(48例);ARDS患儿根据预后情况分为预后不良组(55例)和预后良好组(69例)。采用荧光定量聚合酶链反应测定血清中lncRNA ANRIL、lncRNA PVT1水平。采用Logistic回归分析法分析ARDS患儿发生预后不良的影响因素;采用受试者工作特征(ROC)曲线分析血清lncRNA ANRIL、lncRNA PVT1水平对ARDS患儿发生预后不良的预测价值。结果患病组的血清lncRNA ANRIL、lncRNA PVT1水平高于对照组,差异有统计学意义(P<0.05)。轻度组、中度组和重度组的血清lncRNA ANRIL、lncRNA PVT1水平随病情严重程度升高(P<0.05)。预后不良组的血清lncRNA ANRIL、lncRNA PVT1水平、氧合指数(OI)、呼吸频率、急性生理与慢性健康状况评分系统Ⅱ(APACHEⅡ)评分高于预后良好组,差异有统计学意义(P<0.05);OI、呼吸频率、lncRNA ANRIL、lncRNA PVT1为患儿发生预后不良的影响因素(P<0.05)。血清lncRNA ANRIL、lncRNA PVT1水平预测ARDS患儿发生预后不良的曲线下面积(AUC)分别为0.827、0.737,截断值分别是11.35、4.36,二者联合预测的AUC为0.876。二者联合预测的AUC优于血清lncRNA ANRIL、lncRNA PVT1水平单独预测(P<0.05)。结论ARDS患儿的血清lncRNA ANRIL、lncRNA PVT1水平均上调,且lncRNA ANRIL、lncRNA PVT1为患儿发生预后不良的影响因素,二者联合预测的价值较高。 展开更多
关键词 急性呼吸窘迫综合征 长链非编码RNA INK4位点反义非编码RNA 长链非编码RNA浆细胞瘤变异易位基因1 儿童 病情 预后
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Herpes simplex virus-1 infection or Simian virus 40-mediated immortalization of corneal cells causes permanent translocation of NLRP3 to the nuclei 被引量:5
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作者 Shu-Long Wang Ge Zhao +5 位作者 Wei Zhu Xiao-Meng Dong Ting Liu Yuan-Yuan Li Wen-Gang Song Yi-Qiang Wang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2015年第1期46-51,共6页
AIM: To investigate into the potential involvement of pyrin containing 3 gene(NLRP3), a member of the nucleotide-binding oligomerization domain-like receptors with cytosolic pattern recognition, in the host defense of... AIM: To investigate into the potential involvement of pyrin containing 3 gene(NLRP3), a member of the nucleotide-binding oligomerization domain-like receptors with cytosolic pattern recognition, in the host defense of corneas against viruses.METHODS: The herpes viral keratitis model was utilized in BALB/c mice with inoculation of herpes simplex virus-1(HSV-1). Corneal tissues removed during therapy of patients with viral keratitis as well as a Simian vacuolating virus 40(SV40)-immortalized human corneal epithelial cell line were also examined.Immunohistochemistry was used to detect NLRP3 in these subjects, focusing on their distribution in tissue or cells. Western blot was used to measure the level of NLRP3 and another two related molecules in NLPR3 inflammasome, namely caspase-1 and IL-1β.RESULTS: The NLRP3 activation induced by HSV-1infection in corneas was accompanied with redistribution of NLRP3 from the cytoplasm to the nucleus in both murine and human corneal epithelial cells. Furthermore,in the SV40-immortalized human corneal epithelial cells,NLRP3 was exclusively located in the nucleus, and treatment of the cells with high concentration of extracellular potassium(known as an inhibitor of NLRP3activation) effectively drove NLRP3 back to the cytoplasm as reflected by both immunohistochemistry and Western blot.· CONCLUSION: It is proposed that herpes virus infection activates and causes redistribution of NLRP3 to nuclei. Whether this NLRP3 translocation occurs with other viral infections and in other cell types merit further study. 展开更多
关键词 pyrin containing 3 gene INFLAMMASOME translocation herpes simplex virus-1 KERATITIS human corneal epithelial cell Simian vacuolating virus 40 IMMORTALIZATION
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Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation 被引量:10
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作者 Jian Fang CHAI Xu LIU Ji Zeng JIA 《Cell Research》 SCIE CAS CSCD 2005年第8期658-664,共7页
Wheat 1BL/1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield. 1BL/1RS translocations are however po... Wheat 1BL/1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield. 1BL/1RS translocations are however poor in bread making, partially caused by a family of small monomeric proteins, ω-secalins, which are encoded by genes on 1RS. Based on published sequence of a rye ω-secalin gene we designed a pair of primers to cover the whole mature protein coding sequence. A major band could be amplified from 1BL/1RS translocations but not from euploid wheat. Using this primer set we conducted PCR amplification by using high fidelity Pfu polymerase on the genomic DNAs and cDNAs purified from a 1BL/1RS translocation Lankao 906. Sequencing analysis indicated that this gene family contains several mem- bers of 1150 bp, 1076 bp, 1075 bp, 1052 bp and 1004 bp genes, including two pseudogenes and three active genes. The gene transcripts were differentially expressed in developing seeds. 展开更多
关键词 WHEAT 1BL/1RS translocation ω-secalin QUALITY homoeologous cloning.
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Lysophosphatidic acid induced nuclear translocation of nuclear factor-κB in Panc-1 cells by mobilizing cytosolic free calcium 被引量:5
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作者 Yoshiyuki Arita Tetsuhide Ito +3 位作者 Takamasa Oono Ken Kawabe Terumasa Hisano Ryoichi Takayanagi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第28期4473-4479,共7页
AIM: To clarify whether Lysophosphatidic acid (LPA) activates the nuclear translocation of nuclear factor-κB (NF-κB) in pancreatic cancer. METHODS: Panc-1, a human pancreatic cancer cell line, was used throughout th... AIM: To clarify whether Lysophosphatidic acid (LPA) activates the nuclear translocation of nuclear factor-κB (NF-κB) in pancreatic cancer. METHODS: Panc-1, a human pancreatic cancer cell line, was used throughout the study. The expression of LPA receptors was confirmed by reverse-transcript polymerase chain reaction (RT-PCR). Cytosolic free calcium was measured by fluorescent calcium indicator fura-2, and the localization of NF-κB was visualized by immunofluorescent method with or without various agents, which effect cell signaling. RESULTS: Panc-1 expressed LPA receptors, LPA1, LPA2 and LPA3. LPA caused the elevation of cytosolic free calcium dose-dependently. LPA also caused the nuclear translocation of NF-κB. Cytosolic free calcium was attenuated by pertussis toxin (PTX) and U73122, an inhibitor of phospholipase C. The translocation of NF-κB was similarly attenuated by PTX and U73122, but phorbol ester, an activator of protein kinase C, alone did not translocate NF-κB. Furthermore, the translocation of NF-κB was completely blocked by Ca2+ chelator BAPTA-AM. Thapsigargin, an endoplasmic- reticulum Ca2+-ATPase pump inhibitor, also promoted the translocation of NF-κB. Staurosporine, a proteinkinase C inhibitor, attenuated translocation of NF-κB induced by LPA. CONCLUSION: These findings suggest that protein kinase C is activated endogenously in Panc-1, and protein kinase C is essential for activating NF-κB with cytosolic calcium and that LPA induces the nuclear translocation of NF-κB in Panc-1 by mobilizing cytosolic free calcium. 展开更多
关键词 Lysophosphatidic acid Nuclear translocation Nuclear factor-κB Cytosolic free calcium PANC-1
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Hepatitis C Virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-α through dephosphorylation 被引量:4
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作者 Guo-Zhong Gong Jie Cao Yong-Fang Jiang Yang Zhou Bo Liu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第30期4080-4084,共5页
AIM: To study the effect of Hepatitis C virus nonstructural 5A (HCV NSSA) on IFNα induced signal transducer and activator of transcription-1 (STAT1) phosphorylation and nuclear translocation.METHODS: Expression... AIM: To study the effect of Hepatitis C virus nonstructural 5A (HCV NSSA) on IFNα induced signal transducer and activator of transcription-1 (STAT1) phosphorylation and nuclear translocation.METHODS: Expression of STAT1 Tyr701 phosphorylation at different time points was confirmed by Western blot, and the time point when p-STAT1 expressed most, was taken as the IFN induction time for further studies. Immunocytochemistry was used to confirm the successful transient transfection of NS5A expression plasmid. Immunofluorescene was performed to observe if there was any difference in IFNα-induced STAT1 phosphorylation and nuclear translocation between HCV NSSA-expressed and non-HCV NSSA-expressed cells. Western blot was used to compare the phosphorylated STAT1 protein of the cells.RESULTS: Expression of HCV NS5A was found in the cytoplasm of pCNS5A-transfected Huh7 cells, but not in the PRC/ CMV transfected or non-transfected cells, STAT1 Tyr701 phosphorylation was found strongest in 30 min of IFN induction, STAT1 phosphorylation and nuclear import were much less in the presence of HCV NS5A protein in contrast to pRC/CMV-transfected and non-transfected cells under fluorescent microscopy, which was further confirmed by Western blot.CONCLUSION: HCV NSSA expression plasmid is successfully transfected into Huh7 cells and HCV NS5A protein is expressed in the cytoplasm of the cells. IFN-α is able to induce STAT1 phosphrylation and nuclear translocation, and this effect is inhibited by HCV NS5A protein, which might be another possible resistance mechanism to interferon alpha therapy. 展开更多
关键词 Hepatitis C virus nonstructural protein 5A IFN-Α Signal transducer and activator of transcription (STAT1 PHOSPHORYLATION Nuclear translocation
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dep1 improves rice grain yield and nitrogen use efficiency simultaneously by enhancing nitrogen and dry matter translocation 被引量:1
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作者 HUANG Li-ying Li Xiao-xiao +2 位作者 ZHANG Yun-bo Shah FAHAD WANG Fei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第11期3185-3198,共14页
The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological ... The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.Therefore,we evaluated the yield performance and NUE of two near-isogenic lines(NILs)carrying DEP1(NIL-DEP1)and dep1-1(NIL-dep1)genes under the Nanjing 6 background at 0 and 120 kg N ha^(–1).Grain yield and NUE for grain production(NUEg)were 25.5 and 21.9%higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years,respectively.The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size(i.e.,higher total spikelet number),grainfilling percentage,total dry matter production,and harvest index.N utilization rather than N uptake contributed to the high yield of NIL-dep1.Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency,lower leaf and stem N concentration at maturity,and higher glutamine synthetase(GS)activity in leaves.In conclusion,dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period. 展开更多
关键词 dep1 grain yield nitrogen use efficiency nitrogen translocation RICE
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circPVT1靶向调控miR-195-5p/SOX9轴影响结肠癌细胞增殖、凋亡及上皮间质转化
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作者 刘光世 李涛 +3 位作者 李鹏 马占军 张旺 袁传威 《现代肿瘤医学》 CAS 2024年第12期2189-2195,共7页
目的:探究环状RNA浆细胞瘤变体易位1(circPVT1)靶向调控微小RNA-195-5p(miR-195-5p)/性别决定区Y框蛋白9(SOX9)轴对结肠癌细胞增殖、凋亡及上皮间质转化(EMT)的影响。方法:体外培养人正常结肠上皮细胞NCM-460、人结肠癌细胞系(SW480、HC... 目的:探究环状RNA浆细胞瘤变体易位1(circPVT1)靶向调控微小RNA-195-5p(miR-195-5p)/性别决定区Y框蛋白9(SOX9)轴对结肠癌细胞增殖、凋亡及上皮间质转化(EMT)的影响。方法:体外培养人正常结肠上皮细胞NCM-460、人结肠癌细胞系(SW480、HCT116、HCT29、LOVO、SW620)至对数期,将SW480细胞分为对照组(正常培养SW480细胞不进行转染)、空载质粒组(转染空载质粒)、circPVT1沉默组[转染circPVT1小干扰RNA(siRNA)质粒]、miR-195-5p过表达组[转染miR-195-5p模拟物(mimics)质粒]、SOX9沉默组(转染SOX9 siRNA质粒)、circPVT1沉默+miR-195-5p低表达组(转染circPVT1 siRNA和miR-195-5p siRNA质粒)。各组转染相应质粒后培养48 h。荧光定量PCR法检测NCM-460细胞、人结肠癌细胞系(SW480、HCT116、HCT29、LOVO、SW620)和各组SW480细胞circPVT1、miR-195-5p、SOX9 mRNA的表达;噻唑蓝和流式细胞仪分别检测各组SW480细胞增殖和凋亡;蛋白印迹法检测各组SW480细胞SOX9、凋亡和EMT相关蛋白表达;双荧光素酶报告基因实验检测circPVT1与miR-195-5p、miR-195-5p与SOX9的靶向关系。结果:与NCM-460细胞比较,SW480细胞、HCT116细胞、HCT29细胞、LOVO细胞、SW620细胞中circPVT1、SOX9 mRNA表达显著升高,miR-195-5p表达显著降低(P<0.05);其中,SW480细胞中circPVT1、SOX9 mRNA表达最高,miR-195-5p表达最低;故选择SW480细胞进行后续实验。与对照组和空载质粒组比较,circPVT1沉默组circPVT1、SOX9 mRNA及蛋白表达显著降低,miR-195-5p表达显著升高(P<0.05);miR-195-5p过表达组miR-195-5p表达显著升高,SOX9 mRNA及蛋白表达显著降低(P<0.05);SOX9沉默组SOX9 mRNA及蛋白表达显著降低(P<0.05)。与对照组和空载质粒组比较,circPVT1沉默组、miR-195-5p过表达组、SOX9沉默组细胞增殖率、Bcl-2、Vimentin蛋白表达显著降低,凋亡率、Bax、E-cadherin蛋白表达显著升高(P<0.05)。与circPVT1沉默组比较,circPVT1沉默+miR-195-5p低表达组miR-195-5p、凋亡率、Bax、E-cadherin蛋白表达显著降低,SOX9 mRNA及蛋白、细胞增殖率、Bcl-2、Vimentin蛋白表达显著升高(P<0.05)。circPVT1与miR-195-5p、miR-195-5p与SOX9存在靶向调控关系。结论:沉默circPVT1可以靶向上调miR-195-5p表达,抑制SOX9表达,进而抑制SW480细胞增殖和EMT进程,促进其凋亡。 展开更多
关键词 环状RNA浆细胞瘤变体易位1 微小RNA-195-5p 性别决定区Y框蛋白9 结肠癌 上皮间质转化
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运动性骨骼肌损伤中时钟基因BMAL1与MyoD的作用
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作者 刘志杨 傅泽铤 +1 位作者 夏雨 丁海丽 《中国组织工程研究》 CAS 北大核心 2024年第4期510-515,共6页
背景:一次大负荷运动后会引起肌联蛋白titin降解导致骨骼肌损伤,成肌调节因子家族MyoD参与骨骼肌生成,在骨骼肌损伤修复中发挥重要的作用。目的:观察一次大负荷运动不同时相下骨骼肌MyoD、时钟基因BMAL1与titin表达变化,以期明确BMAL1与... 背景:一次大负荷运动后会引起肌联蛋白titin降解导致骨骼肌损伤,成肌调节因子家族MyoD参与骨骼肌生成,在骨骼肌损伤修复中发挥重要的作用。目的:观察一次大负荷运动不同时相下骨骼肌MyoD、时钟基因BMAL1与titin表达变化,以期明确BMAL1与MyoD在运动诱导骨骼肌损伤中的作用。方法:24只8周龄SD大鼠随机分为安静对照组(n=4)和运动组(n=20)。运动组大鼠于跑台进行90 min下坡跑,运动后即刻(0 h)及运动后12,24,48,72 h取比目鱼肌。通过实时荧光定量PCR实验检测BMAL1、MyoD的mRNA表达量;透射电镜观察骨骼肌肌纤维超微结构变化;免疫荧光观测MyoD与BMAL1、BMAL1与titin的定位情况。结果与结论:①透射电镜显示:一次大负荷离心运动后,大鼠比目鱼肌部分位置肌节变宽,Z线模糊不清呈水波状,其中运动后12 h损伤最为严重,72 h后基本恢复;②实时荧光定量PCR检测显示:运动组BMAL1的mRNA表达呈现先升高,后趋于正常的状态;MyoD的mRNA表达呈现先下降、后升高的趋势;③免疫荧光观测:运动组可在12,24 h观测到BMAL1和MyoD的共定位;可在0,12,24 h观测到BMAL1和titin的共定位;④结果表明,MyoD与BMAL1共同参与运动性骨骼肌损伤的修复,可能是通过titin进行的。 展开更多
关键词 运动性骨骼肌损伤 运动 骨骼肌 损伤 成肌调节因子 大脑/肌肉芳香经受体核转位因子样蛋白1 骨架蛋白 肌联蛋白
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Microbial translocation, residual viremia and immune senescence in the pathogenesis of HIV-1 infection
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作者 Alessandra Fantauzzi Francesca Falasca +4 位作者 Gabriella d’Ettorre Eugenio Nelson Cavallari Ombretta Turriziani Vincenzo Vullo Ivano Mezzaroma 《World Journal of Clinical Infectious Diseases》 2013年第4期47-57,共11页
The pathophysiological mechanisms that underlie the progression of human immunodeficiency virus-1(HIV-1) disease to full-blown AIDS are not well understood. Findings suggest that, during HIV-1 infection, plasma lipopo... The pathophysiological mechanisms that underlie the progression of human immunodeficiency virus-1(HIV-1) disease to full-blown AIDS are not well understood. Findings suggest that, during HIV-1 infection, plasma lipopolysaccharide(LPS) levels, which are used as an indicator of microbial translocation(MT), are elevated throughout the acute and chronic phases of HIV-1 disease. The translocation of bacterial products through the damaged gastrointestinal barrier into the systemic circulation has been described as a driver of immune activation. In contrast, comorbidities that are associated with HIV-1 infection have been attributed to chronic inflammation and immune system dysfunction secondary to MT or low-level HIV-1 replication in plasma and cell reservoirs. Moreover, accelerated aging is significantly associated with chronic inflammation, immune activation, and immune senescence. In this review, we aimed to investigate the role of inflammation as a pivotal marker in the pathogenesis of HIV-1 disease. We will discuss the key features of chronic inflammation and immune activation that are observed during the natural course of the disease and those features that are detected in c ART-modified infection. The review will focus on the following aspects of HIV-1 infection:(1) MT;(2) the role of residual viremia; and(3) "immune senescence" or "inflammaging." Many questions remain unanswered about the potential mechanisms that are involved in HIV-1 pathogenesis. Further studies are needed to better investigate the mechanisms that underlie immune activation and their correlation with HIV-1 disease progression. 展开更多
关键词 Human IMMUNODEFICIENCY virus-1 Combined ANTIRETROVIRAL therapy IMMUNE activation Microbial translocation RESIDUAL VIREMIA IMMUNE senescence
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BMAL1和miR-552在胃癌中的表达及其临床价值
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作者 吕赛华 张兴 《国际消化病杂志》 CAS 2024年第5期296-301,共6页
目的探讨胃癌中芳香烃受体核转运体样蛋白1(BMAL1)和miR-552的表达及其临床价值。方法选择2018年1月至2019年1月在绍兴文理学院附属医院诊治的92例胃癌患者设为胃癌组,40例胃良性疾病患者设为对照组。收集胃癌组的胃癌组织和癌旁组织、... 目的探讨胃癌中芳香烃受体核转运体样蛋白1(BMAL1)和miR-552的表达及其临床价值。方法选择2018年1月至2019年1月在绍兴文理学院附属医院诊治的92例胃癌患者设为胃癌组,40例胃良性疾病患者设为对照组。收集胃癌组的胃癌组织和癌旁组织、对照组的胃病灶组织。另选取正常胃黏膜上皮细胞RGM-1和胃癌细胞(AGS、Hs746T、MGC-803及SGC-7901)。采用实时荧光定量PCR法检测各种组织和细胞中BMAL1和miR-552表达水平。分析胃癌组织中BMAL1和miR-552表达与患者临床病理特征的关系。采用ROC曲线分析胃癌组织中BMAL1和miR-552表达对患者死亡的预测效能。采用Kaplan-Meier生存曲线分析BMAL1和miR-552表达与胃癌患者生存期的关系。采用多因素logistic回归分析探讨胃癌患者预后的危险因素。结果胃癌组织中BMAL1和miR-552表达水平均显著高于癌旁组织和对照组胃病灶组织(P均<0.05),各种胃癌细胞中BMAL1和miR-552表达水平均显著高于正常胃黏膜上皮细胞(P均<0.05)。低分化、肿瘤最大径≥5 cm、T3~T4、有淋巴结转移及TNM分期Ⅲ~Ⅳ期患者的BMAL1和miR-552表达水平分别显著高于中~高分化、肿瘤最大径<5 cm、T1~T2、无淋巴结转移及TNM分期Ⅰ~Ⅱ期患者。BMAL1预测胃癌患者死亡的截断值为2.0,敏感度为81.2%,特异度为93.6%(P<0.05);miR-552预测胃癌患者死亡的截断值为1.0,敏感度为97.6%,特异度为74.2%(P<0.05)。低分化、肿瘤最大径≥5cm、T3~T4、有淋巴结转移、TNM分期Ⅲ~Ⅳ期、BMAL1表达≥2.0、miR-552表达≥1.0均为胃癌患者预后的危险因素(P均<0.05)。胃癌组织中BMAL1表达≥2.0且miR-552表达≥1.0的患者的中位生存期显著短于BMAL1表达<2.0或miR-552表达<1.0的患者[中位生存期(25.3±5.2)个月比(31.4±6.6)个月,Log-rank=14.677,P<0.05]。结论胃癌组织中BMAL1和miR-552表达水平均显著升高,其与患者的组织学分级、肿瘤最大径、T分期、淋巴结转移、TNM分期及生存期均有相关性。BMAL1表达≥2.0、miR-552表达≥1.0均为胃癌患者预后的危险因素,两者有潜力作为胃癌患者病情及预后评估的标志物。 展开更多
关键词 胃癌 芳香烃受体核转运体样蛋白1 miR-552 临床价值
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IS患者血清lncRNA PVT1、miR-214水平与继发性癫痫的相关性分析
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作者 郭舒雯 赵雄飞 代昌飞 《脑与神经疾病杂志》 2024年第1期17-21,共5页
目的 探讨缺血性脑卒中(IS)患者血清长链非编码RNA变异性浆细胞瘤异位1 (lncRNA PVT1)、微小RNA-214 (miR-214)水平与继发性癫痫的相关性。方法 选取2018年6月至2021年6月眉山心脑血管病医院神内八科收治的280例IS患者作为研究对象,根... 目的 探讨缺血性脑卒中(IS)患者血清长链非编码RNA变异性浆细胞瘤异位1 (lncRNA PVT1)、微小RNA-214 (miR-214)水平与继发性癫痫的相关性。方法 选取2018年6月至2021年6月眉山心脑血管病医院神内八科收治的280例IS患者作为研究对象,根据患者1年内是否发生继发性癫痫,分为单纯IS组(212例)和继发性癫痫组(68例),根据癫痫的严重程度分为轻度癫痫组(19例),中度癫痫组(33例),重度癫痫组(16例)。另选取同时期来眉山心脏血管病医院神经八科体检的150例健康者作为对照组。采用实时荧光定量PCR(qRT-PCR)法测定所有受试者血清lncRNA PVT1、miR-214水平;采用酶联免疫吸附试验(ELISA)法检测血清中白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)水平;经Pearson法分析lncRNA PVT1与miR-214的相关性,及两者与IL-1 β、IL-6、TNF-α的相关性;采用多因素Logistic回归分析影响IS患者是否发生继发性癫痫的有关因素。结果 与对照组比较,单纯IS组和继发性癫痫组患者血清lncRNAPVT1水平明显升高,血清miR-214水平明显降低(P<0.05);与单纯IS组比较,继发性癫痫组患者血清lncRNA PVT1水平明显升高,血清miR-214水平明显降低(P<0.05)。与轻度癫痫组比较,中度癫痫组和重度癫痫组患者血清lncRNA PVT1水平明显升高,血清miR-214水平明显降低(P<0.05);与中度癫痫组比较,重度癫痫组患者血清lncRNA PVT1水平明显升高,血清miR-214水平明显降低(P <0.05)。继发性癫痫组患者血清lncRNA PVT1与IL-1 β、IL-6、TNF-α均呈正相关(P <0.05),血清miR-214与IL-1 β、IL-6、TNF-α均呈负相关(P <0.05),血清lncRNA PVT1与miR-214水平也呈负相关(P <0.05)。多因素Logistic回归分析表明,IL-1 β、IL-6、TNF-α、lncRNA PVT1及miR-214均与IS患者继发性癫痫的发生有关(P <0.05)。结论lncRNA PVT1水平升高及miR-124水平降低与IS患者继发性癫痫的发生有关,可作为评估IS患者发生继发性癫痫的血清标志物。 展开更多
关键词 缺血性脑卒中 继发性癫痫 长链非编码RNA变异性浆细胞瘤异位1 微小RNA-214
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敲低黏膜相关淋巴组织淋巴瘤易位蛋白1对大鼠脊髓损伤炎症因子表达及神经功能的影响
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作者 黄伟明 陈宏权 +2 位作者 张俊鑫 张清平 柳清洋 《中国微侵袭神经外科杂志》 CAS 2024年第10期603-608,共6页
目的 探讨黏膜相关淋巴组织淋巴瘤易位蛋白1(mucosa associated lymphoid tissue lymphoma translocation protein1,MALT1)对大鼠脊髓损伤(spinal cord injury,SCI)后神经炎症及神经功能恢复的影响。方法 选取96只SD(Sprague Dawley)大... 目的 探讨黏膜相关淋巴组织淋巴瘤易位蛋白1(mucosa associated lymphoid tissue lymphoma translocation protein1,MALT1)对大鼠脊髓损伤(spinal cord injury,SCI)后神经炎症及神经功能恢复的影响。方法 选取96只SD(Sprague Dawley)大鼠,随机分成普通组及MALT1敲低组,普通组分为假手术组(n=24)、脊髓损伤组(SCI组)(n=24)。MALT1敲低组分为假手术组(n=12)、SCI组(n=12)、SCI注射慢病毒阴性对照组(SCI+NC组)(n=12)、SCI注射慢病毒MALT1敲除组(SCI+KD组)(n=12)。利用大鼠BBB(Basso、Beattie和Bresnahan,BBB)评分评估大鼠运动能力,苏木素-伊红(hematoxylin-eosin,HE)染色明确SCI程度,免疫蛋白质印迹法检测各组小鼠脊髓白细胞介素6(interleukin-6,IL-6)、白细胞介素10(interleukin-10,IL-10)、肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)和MALT1的表达。结果 与假手术组相比,SCI组大鼠MALT1表达、神经炎症指标均较高,而运动能力较差(均P<0.05)。在SCI+KD组大鼠中,MALT1敲低在造模后10~28d改善大鼠BBB评分,减轻神经损伤(P<0.05),炎症因子IL-1β、IL-6和TNF-α表达下降(均P<0.05),但不影响IL-10表达(P>0.05)。结论 MALT1敲低通过抑制SCI炎症因子表达,可改善脊髓功能恢复。 展开更多
关键词 黏膜相关淋巴组织淋巴瘤易位蛋白1 脊髓损伤 炎症因子 神经功能
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PVT1通过TGF-β1/SMAD通路促进子宫内膜基质细胞过度纤维化的研究
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作者 杨强 张志伟 +1 位作者 邱娟娟 王雨潇 《中国性科学》 2024年第1期82-86,共5页
目的探讨长链非编码RNA(lncRNA)浆细胞瘤多样异位基因1(PVT1)在调控子宫内膜基质细胞纤维化中的病理生理作用及分子机理。方法通过实时荧光定量聚合酶链反应(qRT-PCR)检测人子宫内膜基质细胞(HESCs)中PVT1、胶原蛋白Ⅰ、胶原蛋白Ⅲ、转... 目的探讨长链非编码RNA(lncRNA)浆细胞瘤多样异位基因1(PVT1)在调控子宫内膜基质细胞纤维化中的病理生理作用及分子机理。方法通过实时荧光定量聚合酶链反应(qRT-PCR)检测人子宫内膜基质细胞(HESCs)中PVT1、胶原蛋白Ⅰ、胶原蛋白Ⅲ、转化生长因子-β1(TGF-β1)、SMAD2及SMAD3的表达水平。结果PVT1过表达促进HESCs增殖(P<0.05),而PVT1沉默抑制HESCs增殖(P<0.05)。过表达PVT1在HESCs中上调胶原蛋白Ⅰ和胶原蛋白ⅢmRNA的表达(P<0.05),而PVT1被敲除时,胶原蛋白Ⅰ和胶原蛋白ⅢmRNA的表达水平被显著下调(P<0.05)。过表达PVT1在HESCs中可以进一步上调TGF-β1/SMAD信号相关基因(TGF-β1、SMAD2、SMAD3)的表达(P<0.05)。当PVT1被敲除时,TGF-β1/SMAD信号相关基因胶原蛋白Ⅰ和胶原蛋白ⅢmRNA的表达水平被显著下调(P<0.05)。PVT1可诱导HESCs上清液中TGF-β1的产生(P<0.05)。结论PVT1可通过TGF-β1/SMAD通路促进子宫内膜基质细胞过度纤维化而产生胶原蛋白。同时,PVT1可能是一个用于治疗子宫内膜粘连的新靶点。 展开更多
关键词 长链非编码RNA 浆细胞瘤多样异位基因1 纤维化 子宫内膜基质细胞 转化生长因子-β1/SMAD通路
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基于lncRNA PVT1表达分析银杏内酯B对ITP患者B淋巴细胞及Treg/Th17免疫失衡的影响
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作者 雷蕊 杨亚丽 +2 位作者 张宝君 张丙寅 王金龙 《检验医学与临床》 CAS 2024年第21期3157-3160,3167,共5页
目的探讨银杏内酯B对原发性免疫血小板减少症(ITP)患者长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)、B淋巴细胞和调节性T淋巴细胞/辅助性T淋巴细胞17(Treg/Th17)免疫失衡的影响及其相关性。方法依据双盲法将2021年5月至2023年5月... 目的探讨银杏内酯B对原发性免疫血小板减少症(ITP)患者长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)、B淋巴细胞和调节性T淋巴细胞/辅助性T淋巴细胞17(Treg/Th17)免疫失衡的影响及其相关性。方法依据双盲法将2021年5月至2023年5月廊坊市中医医院收治的130例ITP患者分为对照组(65例)和观察组(65例)。对照组给予长春地辛治疗,观察组给予银杏内酯B治疗,两组均治疗4周。比较两组患者临床疗效;比较两组患者治疗前后lncRNA PVT1表达、B淋巴细胞亚群[CD19^(+)、B1淋巴细胞]比例、Treg/Th17免疫失衡细胞因子[白细胞介素-17(IL-17)、白细胞介素-21(IL-21)];分析lncRNA PVT1表达与CD19^(+)、B1淋巴细胞比例以及IL-17、IL-21水平的相关性。结果研究过程中对照组失访7例,观察组失访5例,最终分组为对照组58例,观察组60例。治疗后两组患者lncRNA PVT1表达、IL-17、IL-21水平以及CD19^(+)、B1淋巴细胞比例较治疗前均有所降低(P<0.05),观察组治疗后lncRNA PVT1表达、IL-17、IL-21水平以及CD19^(+)、B1淋巴细胞比例明显低于对照组(P<0.05)。与对照组相比,观察组临床疗效更优(P<0.05)。lncRNA PVT1表达与CD19^(+)、B1淋巴细胞、IL-17、IL-21水平均呈正相关(P<0.05)。结论银杏内酯B对ITP患者具有较高临床疗效,可以显著改善其lncRNA PVT1表达、B淋巴细胞比例、Treg/Th17免疫失衡细胞因子水平,值得临床推广。 展开更多
关键词 长链非编码RNA浆细胞瘤转化迁移基因1 银杏内酯B 原发性免疫血小板减少症 B淋巴细胞 调节性T淋巴细胞 辅助性T淋巴细胞
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LncRNA PVT1在肝癌中的研究进展 被引量:1
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作者 唐晓婷 彭敦煌 房太勇 《航空航天医学杂志》 2023年第11期1347-1349,共3页
肝癌是我国常见的恶性肿瘤之一,死亡率亦很高,给我们国家带来了极大的经济社会负担。近年的研究发现长链非编码RNA(long noncoding RNA,lncRNA)参与了肝癌的细胞增殖、迁移、侵袭、转移等过程。本文对lncRNA浆细胞瘤变异易位基因1(plasm... 肝癌是我国常见的恶性肿瘤之一,死亡率亦很高,给我们国家带来了极大的经济社会负担。近年的研究发现长链非编码RNA(long noncoding RNA,lncRNA)参与了肝癌的细胞增殖、迁移、侵袭、转移等过程。本文对lncRNA浆细胞瘤变异易位基因1(plasmacytoma variant translocation gene 1,PVT1)概述的基础上,重点阐述lncRNA PVT1在肝癌的发生、进展、转移等方面的作用。 展开更多
关键词 长链非编码码RNA 浆细胞瘤变异易位基因1 原发性肝癌
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Nickel-catalyzed arylcyanation of alkenes via cyano group translocation:access to 1,n-dinitriles or 4-amino nitriles
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作者 Xiaohong Li Shenfan Wang +5 位作者 Xiangxiang Fu Donghui Xing Zeyuan Fu Yuanfu Deng Huanfeng Jiang Liangbin Huang 《Science China Chemistry》 SCIE EI CAS CSCD 2024年第9期2975-2981,共7页
Herein,a nickel-catalyzed arylcyanation of unactivated alkenes via cyano group translocation with aryl boronic acids has been developed.These transformations provided a robust approach to constructing structurally div... Herein,a nickel-catalyzed arylcyanation of unactivated alkenes via cyano group translocation with aryl boronic acids has been developed.These transformations provided a robust approach to constructing structurally diverse 1,n-dinitriles or 4-amino nitriles from easily prepared and commercially available starting materials.The cyano group translocation was achieved,involving the addition into the intramolecular C–N triple bond followed by the retro-Thorpe reaction.Mechanistic studies revealed that high temperature and CsHCO_(3) as the base were crucial for the cyano group translocation. 展开更多
关键词 arylcyanation nickel catalysis cyano group translocation 1 n-dinitriles 4-amino nitriles
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CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation 被引量:4
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作者 Hui-Hong Zhai Juan Meng +3 位作者 Jing-Bo Wang Zhen-Xiong Liu Yuan-Fei Li Shan-Shan Feng 《World Journal of Gastroenterology》 SCIE CAS 2014年第29期10062-10070,共9页
AIM: To investigate the role of nuclear translocation of calcyclin binding protein, also called Siah-1 interacting protein (CacyBP/SIP), in gastric carcinogenesis.
关键词 Calcyclin binding protein/Siah-1 interacting protein Gastric caner cells Nuclear translocation GASTRIN
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