To investigate the effects of different tenascin-R fragments on morphological changes of neurons in vitro. Methods: Cell suspension was prepared from E14-15 mouse embryo spinal cords by mechanical dissection and tryps...To investigate the effects of different tenascin-R fragments on morphological changes of neurons in vitro. Methods: Cell suspension was prepared from E14-15 mouse embryo spinal cords by mechanical dissection and trypsin digestion. The cells were cultured in dishes coated with different bacterial expressed tenascin--R fragments. After being cultured in serum-free medium for 26 h, the cells were fixed and stained by ABC immunocytochemistry method for NSE. The cell number and neurites length were measured by a stereological method using an image analysis system, and the data was analyzed statistically. Results:The cells grew well in the serum-free medium for 26 h. Three types of cells were identified: ①phase-bright cells with single or double neurites; ②phasedark cells with well branching neurites; ③flat cells with 2-4 round vesicles in the cell body and radiotlike neurites. The cell number and the neurites length were influenced by different tenascin-R fragments. It was found that FN1-2 fragment inhibited neurite outgrowth. Conclusion: Different tenascin-R fragments that were used as substrate exert varying effects on cultured neural cells, adhesion or anti-adhension of cells, promotion or inhibition of the growth of neurites. These influences were mediated through receptors on the cell membrane. This study may provide some clues to the search for the search for the different receptors which may play essential roles in neuronal development and plasticity.展开更多
文摘To investigate the effects of different tenascin-R fragments on morphological changes of neurons in vitro. Methods: Cell suspension was prepared from E14-15 mouse embryo spinal cords by mechanical dissection and trypsin digestion. The cells were cultured in dishes coated with different bacterial expressed tenascin--R fragments. After being cultured in serum-free medium for 26 h, the cells were fixed and stained by ABC immunocytochemistry method for NSE. The cell number and neurites length were measured by a stereological method using an image analysis system, and the data was analyzed statistically. Results:The cells grew well in the serum-free medium for 26 h. Three types of cells were identified: ①phase-bright cells with single or double neurites; ②phasedark cells with well branching neurites; ③flat cells with 2-4 round vesicles in the cell body and radiotlike neurites. The cell number and the neurites length were influenced by different tenascin-R fragments. It was found that FN1-2 fragment inhibited neurite outgrowth. Conclusion: Different tenascin-R fragments that were used as substrate exert varying effects on cultured neural cells, adhesion or anti-adhension of cells, promotion or inhibition of the growth of neurites. These influences were mediated through receptors on the cell membrane. This study may provide some clues to the search for the search for the different receptors which may play essential roles in neuronal development and plasticity.
