A single-phase lattice Boltzmann model with modified surface tension is developed in this paper to solve the problem of high-density-ratio free surface flow.The computational efficiency and accuracy are both enhanced....A single-phase lattice Boltzmann model with modified surface tension is developed in this paper to solve the problem of high-density-ratio free surface flow.The computational efficiency and accuracy are both enhanced.The restriction to the relaxation factor (which needs to be smaller than 1) is circumvented by the new surface tension algebra,due to its rational physical nature compared with the treatment of Xing,Buther and Yang in their paper (Comp.Mater.Sci.,2007,39(2):282-290).The proposed stable surface tension scheme is applied to simulate the free deformation of a square droplet with surface tension effect and the process of a droplet impinging on a liquid film.The numerical solution for free deformation of a droplet agrees well with thermodynamic principles,and also achieves high accuracy in comparison with Xing,et al.'s model.Three typical impinging modes are successfully obtained with the new scheme,and another particular mode found by Wang and Chen is also successfully simulated.The evolutions of liquid crown agree well with the power law related to time.展开更多
AIM:To analyze the clinical features,diagnostic modalities,and the surgical management of urethral complications after tension-free vaginal tape procedures.METHODS:This study encompasses a retrospective review of nine...AIM:To analyze the clinical features,diagnostic modalities,and the surgical management of urethral complications after tension-free vaginal tape procedures.METHODS:This study encompasses a retrospective review of nine patients presented with urethral complications after midurethral sling procedures.The patients underwent the procedures during a period from 1999 to 2012 in three different regional hospitals in the southwest part of Sweden.The time from sling placement to diagnosis,the risk factors,clinical features,diagnosis,surgical management,and functional outcome are presented.The presenting symptoms were described as either early onset(< 12 mo) or late onset(> 12 mo) according to when they were first reported.RESul TS:Eight cases of urethral erosion and one case of bladder-neck erosion were detected.The mean interval for diagnoses of the erosions ranged from 3 mo to 11 years.The most common presenting symptoms included de novo urgency with or without incontinence(7/9 patients),urinary retention/voiding dysfunction(4/9 patients),urethritis(4/9 patients),relapse of stress-incontinence(3/9 patients),recurrent urinary tract infections(5/9 patients),and hematuria(1/9 patient).In most cases,voiding dysfunction and urethritis occurred early after the operation.The surgical management applied in most cases was transurethral resection of the intraurethral part of the mesh.The removal of the intraurethral mesh resulted in improvement or complete cure of urgency symptomsin 5/7 patients with urgency.Four patients were reoperated with a new stress-incontinence surgery,one with laparoscopic Burch,and three with retropubic tension-free vaginal tape procedures.COn Clu SIOn:Urethral complications should be suspected in the case of de novo urgency and relapse of stressincontinence.Transurethral excision of the intraurethral mesh is the recommended treatment.展开更多
Objective To study the outcome of tension-free vaginal tape (TVT) for the treatment of stress urinary incontinence (SUI) in women with cystocele. Methods Forty-two patients with SUI confirmed by urodynamics underwent ...Objective To study the outcome of tension-free vaginal tape (TVT) for the treatment of stress urinary incontinence (SUI) in women with cystocele. Methods Forty-two patients with SUI confirmed by urodynamics underwent the TVT procedure under local anesthesia. A prolapse repair was done simultaneously. Results Mean TVT operation time was 26.29 minutes. Mean blood loss was 29.86 mL. Eighty-eight percent of the patients were able to micturate spontaneously within 12 hours and residual urine was less than 100 mL. And 12% of the patients had to use indwelling catheter for 3-11 days. Average hospital stay was 2.91 days. Eighty-eight percent of patients were discharged within 2 days. All patients were followed up (an average of 10.26 months). According to subjective and objective assessment of the outcome, 39 patients (93%) were cured, another 3 patients (7%) were significantly improved and none was failed. There were no major complications such as bladder injury occurred. Conclusion TVT procedure is a minimal invasive, effective, and safe surgery for treatment of SUI.展开更多
目的:探讨腹腔镜经腹膜前腹股沟疝修补术(TAPP)对腹股沟疝患者疼痛程度及皮质醇(Cor)、组织金属蛋白酶抑制物-2(TIMP-2)的影响。方法:选取2017年1月—2022年12月常熟市第五人民医院收治的102例腹股沟疝患者。根据随机数表法将其分为观...目的:探讨腹腔镜经腹膜前腹股沟疝修补术(TAPP)对腹股沟疝患者疼痛程度及皮质醇(Cor)、组织金属蛋白酶抑制物-2(TIMP-2)的影响。方法:选取2017年1月—2022年12月常熟市第五人民医院收治的102例腹股沟疝患者。根据随机数表法将其分为观察组和对照组,各51例。对照组给予传统腹股沟疝无张力修补术,观察组给予TAPP。比较两组围手术期指标,术后1 h、6 h、12 h、24 h疼痛程度,术前、术后24 h相关指标及并发症。结果:观察组术中出血量少于对照组,住院时间短于对照组,差异有统计学意义(P<0.05)。两组视觉模拟评分法(VAS)评分组间、时间、交互比较,差异有统计学意义(P<0.05)。与术后1 h比较,两组术后6 h、12 h VAS评分升高,对照组术后24 h VAS评分升高;与术后6 h比较,两组术后12 h、24 h VAS评分降低,与术后12 h比较,两组术后24 h VAS评分降低,观察组术后6 h、12 h、24 h VAS评分低于对照组,差异有统计学意义(P<0.05)。术后24 h,两组Cor水平升高,TIMP-2水平降低,观察组TIMP-2、Cor水平均低于对照组,差异有统计学意义(P<0.05)。观察组并发症发生率低于对照组,差异有统计学意义(P<0.05)。结论:与传统腹股沟疝无张力修补术比较,TAPP治疗腹股沟疝可减少术中出血量,缩短住院时间,缓解患者的疼痛感,调节Cor、TIMP-2水平,并且降低患者术后并发症发生率。展开更多
The dynamic problem of three-dimensional free surface is numerically studied in this paper. The ALE (Arbitrary Lagrange-Euler) kinematic description is introduced into the control equation system. The ALE descriptio...The dynamic problem of three-dimensional free surface is numerically studied in this paper. The ALE (Arbitrary Lagrange-Euler) kinematic description is introduced into the control equation system. The ALE description method is used to track free surface. Accurate formulations for calculating the normal vector on the free surface are presented. The discrete numerical equations by finite element method are developed by Galerkin weighted residual method. The boundary condition about free-surface tension is represented in the form of weak integration that can be computed by a differential geometry method derived in the present paper. The effect of contact angle is incorporated in the numerical algorithm. Furthermore, the numerical computations are performed and the comparison between computational and analytical results validated the effectiveness of the method. The results of this paper provide a fundamental understandings of the dynamics of liquid free surfaces, in which the surface tension and contact angle boundary conditions are taken into account. Finally, numerical simulation of largescale amplitude sloshing of liquid in a cylindrical container is performed and a numerical analysis of the effect of an annular ring-shaped rigid damping baffle on liquid sloshing oscillations in a cylindrical tank is also carried out.展开更多
Maximum bubble pressure measurement was employed to evaluate surface tension of Sn-8Zn-3Bi- (00.15)Nd and Sn-8Zn-3Bi-(00.15)La solder melts. Wetting balance method was used to measure wetting force and wetting time on...Maximum bubble pressure measurement was employed to evaluate surface tension of Sn-8Zn-3Bi- (00.15)Nd and Sn-8Zn-3Bi-(00.15)La solder melts. Wetting balance method was used to measure wetting force and wetting time on Cu substrate of the two group solders. The experimental results show that minute amount of Nd or La addition to Sn-8Zn-3Bi solder causes significant decrease of the surface tension of the solder melts at 200240℃ and Nd addition is more effective on reduction of surface tension than that of La. Nd or La addition has the effect on enhancing the wetting force of the solder melts on Cu substrate, which results from the decrease of interfacial tension between the solder melt and Cu substrate. The wetting force reaches the maximum when 0.1% Nd is added to the base alloy. The contact angle between Sn-8Zn-3Bi base solders and Cu substrate decreases with the addition of Nd or La and the minimum of the contact angle is obtained from the solder with 0.1% Nd addition.展开更多
Steel 20Mn23AlV is a type of high aluminum steel with a very low ladle free-opening rate. The aluminum composition of 20Mn23AlV ranges from 1.6% to 2.45% ,which is significantly higher than other types of steel. Accor...Steel 20Mn23AlV is a type of high aluminum steel with a very low ladle free-opening rate. The aluminum composition of 20Mn23AlV ranges from 1.6% to 2.45% ,which is significantly higher than other types of steel. According to the real condition of 40 t ladle in steel-making plant of Baosteel Special Steel Company, previous works show that the key factors affecting the ladle free-opening rate of high aluminum steel in continuous casting are:sand material, accessories baking, ladle nozzle cleaning, the process and amount of adding sand, and the rate of argon stirring during refining. Therefore, improving the ladle filler sand quality, baking all of the raw materials, controlling the addition of ladle filler sand, cleaning the ladle nozzle, and optimizing argon stirring during the refining process can resolve the problem of a low ladle free-opening rate of high aluminum steel caused by the long ladle time of liquid steel.展开更多
We have been developing debris-free laser plasma sources for EUV lithography since 1996. Two types of debris-free sources, such as cryogenic target and gas-puff target laser plasma sources, were designed and built up ...We have been developing debris-free laser plasma sources for EUV lithography since 1996. Two types of debris-free sources, such as cryogenic target and gas-puff target laser plasma sources, were designed and built up in CIOMP. EUV radiation spectra of the sources with a variety of targets have been obtained by different ways.展开更多
BACKGROUND Large abdominal wall defect(LAWD)caused by shotgun wound is rarely reported.CASE SUMMARY Herein,we describe a case of LAWD caused by a gunshot wound in which the abdominal wall was reconstructed in stages,i...BACKGROUND Large abdominal wall defect(LAWD)caused by shotgun wound is rarely reported.CASE SUMMARY Herein,we describe a case of LAWD caused by a gunshot wound in which the abdominal wall was reconstructed in stages,including debridement,tensionreduced closure(TRC),and reconstruction with mesh and a free musculocutaneous flap.During a 3-year follow-up,the patient recovered well without hernia or other problems.CONCLUSION TRC is a practical approach for the temporary closure of LAWD,particularly in cases when one-stage abdominal wall restoration is unfeasible due to significant comorbidities.展开更多
In this paper,we give all-sided pastic analysis of the rectangular slab with three edges simply-supported and other free.Here we discuss the following four cases:(1)The uniformly distributedload over the area a slab.(...In this paper,we give all-sided pastic analysis of the rectangular slab with three edges simply-supported and other free.Here we discuss the following four cases:(1)The uniformly distributedload over the area a slab.(2).A concentrated load act at midpoint of free edges slab.(3)A concen-trated load act at the center a slab.(4)The line load act along free edge of slab.展开更多
BACKGROUND It is known that under conditions of tissue tolerance to insulin,observed during type 2 diabetes mellitus(DM2),there is an increased production of reactive oxygen species.Moreover,the free radicals can init...BACKGROUND It is known that under conditions of tissue tolerance to insulin,observed during type 2 diabetes mellitus(DM2),there is an increased production of reactive oxygen species.Moreover,the free radicals can initiate lipid peroxidation(LPO)in lipoprotein particles.The concentration of LPO products can influence the state of insulin receptors,repressing their hormone connection activity,which is expressed as a reduction of the glucose consumption by cells.It is possible that reduction in glucose concentration during administration of 10-(6-plastoquinonyl)decyltriphenylphosphonium(SkQ1)to rats with DM2 may be related to the antioxidant properties of this substance.AIM To establish the influence of SkQ1 on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia.METHODS To induce hyperglycemia,rats were fed a high-fat diet for 1 mo and then administered two intra-abdominal injections of streptozotocin with a 7-d interval at a 30 mg/kg of animal weight dose with citrate buffer equal to pH 4.4.SkQ1 solution was administered intraperitoneally at a 1250 nmol/kg dose per day.Tissue samples were taken from control animals,animals with experimental hyperglycemia,rats with streptozotocin-induced glycemia that were administered SkQ1 solution,animals housed under standard vivarium conditions that were administered SkQ1,rats that were administered intraperitoneally citrate buffer equal to pH 4.4 once a week during 2 wk after 1-mo high-fat diet,and animals that were administered intraperitoneally with appropriate amount of solution without SkQ1(98%ethanol diluted eight times with normal saline solution).To determine the intensity of free radical oxidation and total antioxidant activity,we used the biochemiluminescence method.Aconitate hydratase(AH),superoxide dismutase,and catalase activities were estimated using the Hitachi U-1900 spectrophotometer supplied with software.The amount of citrate was determined by means of the Natelson method.Real-time polymerase chain reaction was carried out using an amplifier ANK-32.RESULTS It was found that the mitochondrial-directed antioxidant elicits decrease of biochemiluminescence parameter values that increase by pathology as well as the levels of primary products of LPO,such as diene conjugates and carbonyl compounds,which indicate intensity of free radical oxidation.At the same time,the activity of AH,considered a crucial target of free radicals,which decreased during experimental hyperglycemia,increased.Apparently,increasing activity of AH influenced the speed of citrate utilization,whose concentration decreased after administering SkQ1 by pathology.Moreover,the previously applied antioxidant during hyperglycemia influenced the rate of antioxidant system mobilization.Thus,superoxide dismutase and catalase activity,as well as the level of gene transcript under influence of SkQ1 at pathology,were changing to the direction of control groups values.CONCLUSION According to the results of performed research,SkQ1 can be considered a promising addition to be included in antioxidant therapy of DM2.展开更多
The effect of strain rate on plasticity has been Investigat-ed for three metallic materials with dynamic tension tech-nique. A phenomenon of high strain induced plasticity -increasing was found for tested materials. T...The effect of strain rate on plasticity has been Investigat-ed for three metallic materials with dynamic tension tech-nique. A phenomenon of high strain induced plasticity -increasing was found for tested materials. The elongation of quenched 30CrMnSiNi2A is 14. 5% under 40 m/s loading rate which is more than two times of the 7 % un-der 0. 5 mm/min. This typic high strain rate induced plasticity - Increasing phenomenon is associated with adiabatic heating, strain twinning and suppression of strain - induced - phase transformation.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos.10625210,50609020 and 10902070)the Leading Academic Discipline Project of Shanghai Municipal Education Commission (Grant No.J50501)
文摘A single-phase lattice Boltzmann model with modified surface tension is developed in this paper to solve the problem of high-density-ratio free surface flow.The computational efficiency and accuracy are both enhanced.The restriction to the relaxation factor (which needs to be smaller than 1) is circumvented by the new surface tension algebra,due to its rational physical nature compared with the treatment of Xing,Buther and Yang in their paper (Comp.Mater.Sci.,2007,39(2):282-290).The proposed stable surface tension scheme is applied to simulate the free deformation of a square droplet with surface tension effect and the process of a droplet impinging on a liquid film.The numerical solution for free deformation of a droplet agrees well with thermodynamic principles,and also achieves high accuracy in comparison with Xing,et al.'s model.Three typical impinging modes are successfully obtained with the new scheme,and another particular mode found by Wang and Chen is also successfully simulated.The evolutions of liquid crown agree well with the power law related to time.
文摘AIM:To analyze the clinical features,diagnostic modalities,and the surgical management of urethral complications after tension-free vaginal tape procedures.METHODS:This study encompasses a retrospective review of nine patients presented with urethral complications after midurethral sling procedures.The patients underwent the procedures during a period from 1999 to 2012 in three different regional hospitals in the southwest part of Sweden.The time from sling placement to diagnosis,the risk factors,clinical features,diagnosis,surgical management,and functional outcome are presented.The presenting symptoms were described as either early onset(< 12 mo) or late onset(> 12 mo) according to when they were first reported.RESul TS:Eight cases of urethral erosion and one case of bladder-neck erosion were detected.The mean interval for diagnoses of the erosions ranged from 3 mo to 11 years.The most common presenting symptoms included de novo urgency with or without incontinence(7/9 patients),urinary retention/voiding dysfunction(4/9 patients),urethritis(4/9 patients),relapse of stress-incontinence(3/9 patients),recurrent urinary tract infections(5/9 patients),and hematuria(1/9 patient).In most cases,voiding dysfunction and urethritis occurred early after the operation.The surgical management applied in most cases was transurethral resection of the intraurethral part of the mesh.The removal of the intraurethral mesh resulted in improvement or complete cure of urgency symptomsin 5/7 patients with urgency.Four patients were reoperated with a new stress-incontinence surgery,one with laparoscopic Burch,and three with retropubic tension-free vaginal tape procedures.COn Clu SIOn:Urethral complications should be suspected in the case of de novo urgency and relapse of stressincontinence.Transurethral excision of the intraurethral mesh is the recommended treatment.
文摘Objective To study the outcome of tension-free vaginal tape (TVT) for the treatment of stress urinary incontinence (SUI) in women with cystocele. Methods Forty-two patients with SUI confirmed by urodynamics underwent the TVT procedure under local anesthesia. A prolapse repair was done simultaneously. Results Mean TVT operation time was 26.29 minutes. Mean blood loss was 29.86 mL. Eighty-eight percent of the patients were able to micturate spontaneously within 12 hours and residual urine was less than 100 mL. And 12% of the patients had to use indwelling catheter for 3-11 days. Average hospital stay was 2.91 days. Eighty-eight percent of patients were discharged within 2 days. All patients were followed up (an average of 10.26 months). According to subjective and objective assessment of the outcome, 39 patients (93%) were cured, another 3 patients (7%) were significantly improved and none was failed. There were no major complications such as bladder injury occurred. Conclusion TVT procedure is a minimal invasive, effective, and safe surgery for treatment of SUI.
文摘目的:探讨腹腔镜经腹膜前腹股沟疝修补术(TAPP)对腹股沟疝患者疼痛程度及皮质醇(Cor)、组织金属蛋白酶抑制物-2(TIMP-2)的影响。方法:选取2017年1月—2022年12月常熟市第五人民医院收治的102例腹股沟疝患者。根据随机数表法将其分为观察组和对照组,各51例。对照组给予传统腹股沟疝无张力修补术,观察组给予TAPP。比较两组围手术期指标,术后1 h、6 h、12 h、24 h疼痛程度,术前、术后24 h相关指标及并发症。结果:观察组术中出血量少于对照组,住院时间短于对照组,差异有统计学意义(P<0.05)。两组视觉模拟评分法(VAS)评分组间、时间、交互比较,差异有统计学意义(P<0.05)。与术后1 h比较,两组术后6 h、12 h VAS评分升高,对照组术后24 h VAS评分升高;与术后6 h比较,两组术后12 h、24 h VAS评分降低,与术后12 h比较,两组术后24 h VAS评分降低,观察组术后6 h、12 h、24 h VAS评分低于对照组,差异有统计学意义(P<0.05)。术后24 h,两组Cor水平升高,TIMP-2水平降低,观察组TIMP-2、Cor水平均低于对照组,差异有统计学意义(P<0.05)。观察组并发症发生率低于对照组,差异有统计学意义(P<0.05)。结论:与传统腹股沟疝无张力修补术比较,TAPP治疗腹股沟疝可减少术中出血量,缩短住院时间,缓解患者的疼痛感,调节Cor、TIMP-2水平,并且降低患者术后并发症发生率。
基金The project supported by the National Natural Science Foundation of China (10272022, 10572022) and the Basic Research Foundation of Beijing Institute of Technology (000Y07). The English text was polished by Yunming Chen.
文摘The dynamic problem of three-dimensional free surface is numerically studied in this paper. The ALE (Arbitrary Lagrange-Euler) kinematic description is introduced into the control equation system. The ALE description method is used to track free surface. Accurate formulations for calculating the normal vector on the free surface are presented. The discrete numerical equations by finite element method are developed by Galerkin weighted residual method. The boundary condition about free-surface tension is represented in the form of weak integration that can be computed by a differential geometry method derived in the present paper. The effect of contact angle is incorporated in the numerical algorithm. Furthermore, the numerical computations are performed and the comparison between computational and analytical results validated the effectiveness of the method. The results of this paper provide a fundamental understandings of the dynamics of liquid free surfaces, in which the surface tension and contact angle boundary conditions are taken into account. Finally, numerical simulation of largescale amplitude sloshing of liquid in a cylindrical container is performed and a numerical analysis of the effect of an annular ring-shaped rigid damping baffle on liquid sloshing oscillations in a cylindrical tank is also carried out.
文摘Maximum bubble pressure measurement was employed to evaluate surface tension of Sn-8Zn-3Bi- (00.15)Nd and Sn-8Zn-3Bi-(00.15)La solder melts. Wetting balance method was used to measure wetting force and wetting time on Cu substrate of the two group solders. The experimental results show that minute amount of Nd or La addition to Sn-8Zn-3Bi solder causes significant decrease of the surface tension of the solder melts at 200240℃ and Nd addition is more effective on reduction of surface tension than that of La. Nd or La addition has the effect on enhancing the wetting force of the solder melts on Cu substrate, which results from the decrease of interfacial tension between the solder melt and Cu substrate. The wetting force reaches the maximum when 0.1% Nd is added to the base alloy. The contact angle between Sn-8Zn-3Bi base solders and Cu substrate decreases with the addition of Nd or La and the minimum of the contact angle is obtained from the solder with 0.1% Nd addition.
文摘Steel 20Mn23AlV is a type of high aluminum steel with a very low ladle free-opening rate. The aluminum composition of 20Mn23AlV ranges from 1.6% to 2.45% ,which is significantly higher than other types of steel. According to the real condition of 40 t ladle in steel-making plant of Baosteel Special Steel Company, previous works show that the key factors affecting the ladle free-opening rate of high aluminum steel in continuous casting are:sand material, accessories baking, ladle nozzle cleaning, the process and amount of adding sand, and the rate of argon stirring during refining. Therefore, improving the ladle filler sand quality, baking all of the raw materials, controlling the addition of ladle filler sand, cleaning the ladle nozzle, and optimizing argon stirring during the refining process can resolve the problem of a low ladle free-opening rate of high aluminum steel caused by the long ladle time of liquid steel.
文摘We have been developing debris-free laser plasma sources for EUV lithography since 1996. Two types of debris-free sources, such as cryogenic target and gas-puff target laser plasma sources, were designed and built up in CIOMP. EUV radiation spectra of the sources with a variety of targets have been obtained by different ways.
文摘BACKGROUND Large abdominal wall defect(LAWD)caused by shotgun wound is rarely reported.CASE SUMMARY Herein,we describe a case of LAWD caused by a gunshot wound in which the abdominal wall was reconstructed in stages,including debridement,tensionreduced closure(TRC),and reconstruction with mesh and a free musculocutaneous flap.During a 3-year follow-up,the patient recovered well without hernia or other problems.CONCLUSION TRC is a practical approach for the temporary closure of LAWD,particularly in cases when one-stage abdominal wall restoration is unfeasible due to significant comorbidities.
文摘In this paper,we give all-sided pastic analysis of the rectangular slab with three edges simply-supported and other free.Here we discuss the following four cases:(1)The uniformly distributedload over the area a slab.(2).A concentrated load act at midpoint of free edges slab.(3)A concen-trated load act at the center a slab.(4)The line load act along free edge of slab.
文摘BACKGROUND It is known that under conditions of tissue tolerance to insulin,observed during type 2 diabetes mellitus(DM2),there is an increased production of reactive oxygen species.Moreover,the free radicals can initiate lipid peroxidation(LPO)in lipoprotein particles.The concentration of LPO products can influence the state of insulin receptors,repressing their hormone connection activity,which is expressed as a reduction of the glucose consumption by cells.It is possible that reduction in glucose concentration during administration of 10-(6-plastoquinonyl)decyltriphenylphosphonium(SkQ1)to rats with DM2 may be related to the antioxidant properties of this substance.AIM To establish the influence of SkQ1 on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia.METHODS To induce hyperglycemia,rats were fed a high-fat diet for 1 mo and then administered two intra-abdominal injections of streptozotocin with a 7-d interval at a 30 mg/kg of animal weight dose with citrate buffer equal to pH 4.4.SkQ1 solution was administered intraperitoneally at a 1250 nmol/kg dose per day.Tissue samples were taken from control animals,animals with experimental hyperglycemia,rats with streptozotocin-induced glycemia that were administered SkQ1 solution,animals housed under standard vivarium conditions that were administered SkQ1,rats that were administered intraperitoneally citrate buffer equal to pH 4.4 once a week during 2 wk after 1-mo high-fat diet,and animals that were administered intraperitoneally with appropriate amount of solution without SkQ1(98%ethanol diluted eight times with normal saline solution).To determine the intensity of free radical oxidation and total antioxidant activity,we used the biochemiluminescence method.Aconitate hydratase(AH),superoxide dismutase,and catalase activities were estimated using the Hitachi U-1900 spectrophotometer supplied with software.The amount of citrate was determined by means of the Natelson method.Real-time polymerase chain reaction was carried out using an amplifier ANK-32.RESULTS It was found that the mitochondrial-directed antioxidant elicits decrease of biochemiluminescence parameter values that increase by pathology as well as the levels of primary products of LPO,such as diene conjugates and carbonyl compounds,which indicate intensity of free radical oxidation.At the same time,the activity of AH,considered a crucial target of free radicals,which decreased during experimental hyperglycemia,increased.Apparently,increasing activity of AH influenced the speed of citrate utilization,whose concentration decreased after administering SkQ1 by pathology.Moreover,the previously applied antioxidant during hyperglycemia influenced the rate of antioxidant system mobilization.Thus,superoxide dismutase and catalase activity,as well as the level of gene transcript under influence of SkQ1 at pathology,were changing to the direction of control groups values.CONCLUSION According to the results of performed research,SkQ1 can be considered a promising addition to be included in antioxidant therapy of DM2.
文摘The effect of strain rate on plasticity has been Investigat-ed for three metallic materials with dynamic tension tech-nique. A phenomenon of high strain induced plasticity -increasing was found for tested materials. The elongation of quenched 30CrMnSiNi2A is 14. 5% under 40 m/s loading rate which is more than two times of the 7 % un-der 0. 5 mm/min. This typic high strain rate induced plasticity - Increasing phenomenon is associated with adiabatic heating, strain twinning and suppression of strain - induced - phase transformation.