Methyl ten-butyl ether （MTBE）, a gasoline additive, possesses serious problems to the environmental health. In the present study, a bacterial culture named A-3 which could effectively degrade MTBE was isolated from ...Methyl ten-butyl ether （MTBE）, a gasoline additive, possesses serious problems to the environmental health. In the present study, a bacterial culture named A-3 which could effectively degrade MTBE was isolated from the MTBE contaminated soil. The isolate was identified as Chryseobacterium sp., a new species capable of degrading MTBE. In order to enhance its degradation ability, selected environment factors were investigated. The results showed that the optimal temperature was in the range of 25-30℃, the pH was 7.0, the inoculum size was 2 × 10^8 CFU/ml and the optimal concentration of MTBE was from 50 to 100 mg/L. The maximum MTBE utilization rate （Vmax） was 102 nmol MTBE/（mg cell protein.h）. Furthermore, it was found that the isolate could also degrade tert-butyl alcohol （TBA）. The degradation rates of TBA were much faster than those of MTBE. The additional TBA would lead to the decrease of the initial MTBE degradation rate and the inhibitory effect of TBA increased with the increase of TBA concentration. Similar protein profiles at least seven peptides were demonstrated after SDS-PAGE analysis of crude extracts obtained from the cells growing in MTBE and TBA culture.展开更多
叔丁醇冻结干燥法用于植物材料,获得了良好的效果。经常规固定的样品完成脱水以后浸泡在叔丁醇中,然后将含醇样品放入电冰箱中冷冻,待叔丁醇凝固后再转移到真空镀膜机的钟罩里,用机械泵抽真空,冻结的醇在低真空中升华后样品得到了干燥。...叔丁醇冻结干燥法用于植物材料,获得了良好的效果。经常规固定的样品完成脱水以后浸泡在叔丁醇中,然后将含醇样品放入电冰箱中冷冻,待叔丁醇凝固后再转移到真空镀膜机的钟罩里,用机械泵抽真空,冻结的醇在低真空中升华后样品得到了干燥。SEM 镜检表明,样品的表面和细胞断裂面及其深层(可见到内质网的双层膜、线粒体的嵴、高尔基体潴泡叠层、核糖体颗粒等细胞器)的三维图象均优于用临界点干燥法所得到的样品图象,叔丁醇冻结干燥法用于植物 SEM 样品制备,是一种操作简单,干燥质量可靠、值得推广的方法。展开更多
基金Project supported by the National Natural Science Foundation of China(No.20276048)the Municipal Natural Science Foundation of Tianjin(No.06YFJMJC06800).
文摘Methyl ten-butyl ether （MTBE）, a gasoline additive, possesses serious problems to the environmental health. In the present study, a bacterial culture named A-3 which could effectively degrade MTBE was isolated from the MTBE contaminated soil. The isolate was identified as Chryseobacterium sp., a new species capable of degrading MTBE. In order to enhance its degradation ability, selected environment factors were investigated. The results showed that the optimal temperature was in the range of 25-30℃, the pH was 7.0, the inoculum size was 2 × 10^8 CFU/ml and the optimal concentration of MTBE was from 50 to 100 mg/L. The maximum MTBE utilization rate （Vmax） was 102 nmol MTBE/（mg cell protein.h）. Furthermore, it was found that the isolate could also degrade tert-butyl alcohol （TBA）. The degradation rates of TBA were much faster than those of MTBE. The additional TBA would lead to the decrease of the initial MTBE degradation rate and the inhibitory effect of TBA increased with the increase of TBA concentration. Similar protein profiles at least seven peptides were demonstrated after SDS-PAGE analysis of crude extracts obtained from the cells growing in MTBE and TBA culture.
文摘叔丁醇冻结干燥法用于植物材料,获得了良好的效果。经常规固定的样品完成脱水以后浸泡在叔丁醇中,然后将含醇样品放入电冰箱中冷冻,待叔丁醇凝固后再转移到真空镀膜机的钟罩里,用机械泵抽真空,冻结的醇在低真空中升华后样品得到了干燥。SEM 镜检表明,样品的表面和细胞断裂面及其深层(可见到内质网的双层膜、线粒体的嵴、高尔基体潴泡叠层、核糖体颗粒等细胞器)的三维图象均优于用临界点干燥法所得到的样品图象,叔丁醇冻结干燥法用于植物 SEM 样品制备,是一种操作简单,干燥质量可靠、值得推广的方法。
