Simultaneous purification of minor components in natural products using twin-column recycling chromatography with a step solvent gradient

The isolation of minor components from complex natural product matrices presents a significant challenge in the field of purification science due to their low concentrations and the presence of structurally similar compounds.This study introduces an optimized twin-column recycling chromatography method for the efficient and simultaneous purification of these elusive constituents.By introducing water at a small flowing rate between the twin columns,a step solvent gradient is created,by which the leading edge of concentration band would migrate at a slower rate than the trailing edge as it flowing from the upstream to downstream column.Hence,the band broadening is counterbalanced,resulting in an enrichment effect for those minor components in separation process.Herein,two target substances,which showed similar peak position in high performance liquid chromatography(HPLC)and did not exceed 1.8%in crude paclitaxel were selected as target compounds for separation.By using the twin-column recycling chromatography with a step solvent gradient,a successful purification was achieved in getting the two with the purity almost 100%.We suggest this method is suitable for the separation of most components in natural produces,which shows higher precision and recovery rate compared with the common lab-operated separation ways for natural products(thin-layer chromatography and prep-HPLC).

Progress on the ultrasonic testing and laser thermography techniques for NDT of tokamak plasma-facing components

During manufacturing and operation, different kinds of defects, e.g., delamination or surface cracks, may be generated in the plasma-facing components (PFCs) of a Tokamak device. To ensure the safety of the PFCs, various kinds of nondestructive testing (NDT) techniques are needed for different defect and failure mode. This paper gives a review of the recently developed ultrasonic testing (UT) and laser thermography methods for inspection of the delamination and surface cracks in PFCs. For monoblock W/Cu PFCs of divertor, the bonding quality at both W-Cu and Cu- CuCrZr interfaces was qualified by using UT with a focus probe during manufacturing. A noncontact, coupling-free and flexible ultrasonic scanning testing system with use of an electromagnetic acoustic transducer and a robotic inspection manipulator was introduced then for the in-vessel inspection of delamination defect in first wall (FW). A laser infrared thermography testing method is highlighted for the on-line inspection of delamination defect in FW through the vacuum vessel window of the Tokamak reactor. Finally, a new laser spot thermography method using laser spot array source was described for the online inspection of the surface cracks in FW.

Packed Supercritical Fluid Chromatography for the Analyses and Preparative Separations of Palm Oil Minor Components

This paper depicts a brief review on the applications of packed supercritical fluid chromatography (SFC) in palm oil analyses and purifications from early 1990s to date. Packed SFC has been used for the analyses of various palm oil components. The analytical separations have also been scaled up to preparative scale that leads to the recovery of high value components from palm oil. This review encompasses both analytical and preparative SFC in the oil palm processing.

Testing Component-Based Software: What It has to do with Design and Component Selection

In a component-based software development life cycle, selection of preexisting components is an important task. Every component that has to be reused has an associated risk of failure of not meeting the functional and non-functional requirements. A component's failure would lead a developer to look for some other alternative of combinations of COTS, in-house and engineered components among possible candidate combinations. This means design itself can readily change. The very process of design of a software system and component selection seems to be heavily dependent on testing results. Instability of design, further, becomes more severe due to requirements change requests. Therefore, this instability of design has to be essentially mitigated by using proper design and testing approaches, otherwise, it may lead to exorbitantly high testing cost due to the repeated testing of various alternatives. How these three activities: Component-based software design, component selection and component-based software testing are interrelated? What process model is most suited to address this concern? This work explores the above questions and their implication in terms of nature of a process model that can be convincing in case of component-based software development.

A case study of blasting vibration attenuation based on wave component characteristics

A typical blasting vibration wave is a composite wave,and its attenuation law is affected by the type of dominant wave component.The purpose of the present study is to establish an attenuation equation of the peak particle velocity(PPV),taking into account the attenuation characteristics of P-,S-and R-waves in the blasting vibration wave.Field blasting tests were carried out as a case to specifically apply the proposed equation.In view of the fact that the discrete properties of rock mass will inevitably cause the uncertainty of blasting vibration,we also carried out a probability analysis of PPV uncertainty,and introduced the concept of reliability to evaluate blasting vibration.The results showed that the established attenuation equation had a higher prediction accuracy,and can be considered as a promising equation implemented on more complex sites.The adopted uncertainty analysis method can comprehensively take account of the attenuation law of blasting vibration measured on site and discrete properties of rock masses.The obtained distribution of the PPV uncertainty factor can quantitatively evaluate the reliability of blasting vibration,which is a powerful and necessary supplement to the PPV attenuation equation.

EMPIRICAL BAYES TEST PROBLEMS OF VARIANCE COMPONENTS IN RANDOM EFFECTS MODEL

Bayes decision rule of variance components for one-way random effects model is derived and empirical Bayes (EB) decision rules are constructed by kernel estimation method. Under suitable conditions, it is shown that the proposed EB decision rules are asymptotically optimal with convergence rates near O(n-1/2). Finally, an example concerning the main result is given.

A Metadata Model Based on Coupling Testing Information to Increase Testability of Component

A software component must be tested every time it is reused in order to assure quality of component itself and system in which it is to be integrated.So how to increase testability of component has become a key technology in the software engineering community.Here a method is introduced to increase component testability.And meanings of component testability and relative effective ways to increase testability are summarized.Then definitions of component coupling testing criterion,DU-I(Definition-Use Information) and OP-Vs(Observation-Point Values)are given.Base on these,a definition-use table is introduced,which includes DU-A and OP-Vs item,to help component testers to understand and observe interior details about component under test better.Then a framework of testable component based on above DU-table is given.These facilities provide ways to detect errors,observe state variables by observation-points based monitor mechanism.Moreover,above methods are applied to our application developed by ourselves before,and some test cases are generated.Then our method is compared with Orso method and Kan method using the same example,presenting the comparison results.The results illustrate the validity of our method,effectively generating test cases and killing more mutants.

Minor Component Analysis-based Landing Forecast System for Ship-borne Helicopter

The general structure of ship-borne helicopter landing forecast system is presented, and a novel ship motion prediction model based on minor component analysis (MCA) is built up to improve the forecast effectiveness. To validate the feasibility of this landing forecast system, time series for the roll, pitch and heave are generated by simulation and then forecasted based on MCA. Simulation results show that ship-borne helicopters can land safely in higher sea condition while carrying on rescue or replenishment tasks at sea in terms of the landing forecast system.

Feasibility of differentiating defect signals of ultrasonic testing for laser weld based on independent component analysis theory

Separating noise from observed signals was studied.When the small defect in the T-shape laser welding joint was inspected by ultrasonic testing system adopting independent component analysis(ICA) theory to process the signals.The principle of automatic ultrasonic testing signals processing and negentropy law of ICA were introduced.The experimental data were processed using relative analysis tools and results showed that the ICA could separate defects signals from noise effectively in laboratory.

Analysis of College Students’ Test Scores Based on Two-Component Mixed Generalized Normal Distribution

In order to improve the fitting accuracy of college students’ test scores, this paper proposes two-component mixed generalized normal distribution, uses maximum likelihood estimation method and Expectation Conditional Maxinnization (ECM) algorithm to estimate parameters and conduct numerical simulation, and performs fitting analysis on the test scores of Linear Algebra and Advanced Mathematics of F University. The empirical results show that the two-component mixed generalized normal distribution is better than the commonly used two-component mixed normal distribution in fitting college students’ test data, and has good application value.

Study on Extraction of Effective Aroma Components from Formula Tobacco by the Supercritical CO2 Extraction Method and Its Aroma Composition

[Objectives]This study was conducted to improve the sensory quality and industrial availability of tobacco extracts.[Methods]The L9(34)design was adopted to carried out an extraction experiment,in which formula tobacco was extracted using supercritical CO2,and the extract was concentrated by vacuum distillation.Through sensory evaluation and chemical analysis,the function determination and chemical composition analysis of the tobacco extracts were carried out,and the optimal supercritical fluid extraction process was finally determined.[Results]The obtained optimal supercritical fluid extraction conditions were as follows:extraction temperature 55℃,extraction pressure 25 MPa,CO2 flow rate of 20 L/h,and entrainer of 95%ethanol.The tobacco extract obtained under the optimal conditions endowed the cigarettes with full and delicate aroma,less irritation and clean aftertaste and made the flavor of the cigarettes overall coordinated and softer,so the sensory quality was significantly improved.[Conclusions]The tobacco extract obtained by the supercritical CO2 extraction method from formula tobacco can effectively improve the quality of cigarettes.

A Review of the Calculation Formula for the Four-component Borehole Strainmeter and Application to Earthquake Cases

Based on the principle formula for the four-component strainmeters, we can directly obtain the specific plane strain, shear strain and azimuthal angle of the principal strain, and the maximum and minimum principal strains calculated afterwards are the indirect result. The problems of practicality of the sensitivity coefficients A and B of plane strain and shear strain are then discussed. Based on this idea, we analyzed the observation data of several four-component borehole strainmeters near the epicenter of the Yiliang M_S5.7 earthquake in 2012 and the Ludian M_S6.5 earthquake in 2014 in the Zhaotong area, Yunnan Province. The results show that the analysis based on the perspective of plane strain and shear strain has an obviously better effect than that based on the component readings, and can directly peel off the respective abnormality of the plane strain and shear strain. In addition, the correlation coefficient curves between measured data of two plane strains show significant anomalies which often occur several days before and during the earthquake.

文献《Validation of commercial fiber optic components for aerospace environments》点评

文章是对一篇SPIE会议文献《用于太空环境的商业光纤器件地面验证方法》的点评。介绍了商业光纤部件从市场采购到准备作为太空飞行部件使用前,在空间环境作用(包括真空、热、振动、辐射)下的地面工艺性合格鉴定试验技术。这项技术由NASA哥达德空间飞行中心(GSFC)创立并命名为"工艺技术有效性保证法",该项技术还包括如何建立商用光纤产品制造工艺性合格鉴定试验验收标准或准则方面的内容。"点评"还针对文献中3个实例之一"水星激光高度仪"试验件的热与振动环境试验规范、试验夹具等重要试验技术信息进行详细介绍。

Component system regression testing method based on CTAM

The emphasis of component system regression testing is retesting of the event interaction between updated components and other components in a system.A component system regression testing method based on a new component testing association model （CTAM） is proposed.First,the modification-affected component groups are identified by the impact analysis on CTAM,and each component in this group is assigned with an influence degree.Then,previous test cases are selected according to the influence degree,to generate the minimal regression test suite.Compared with traditional methods,CTAM is derived from the statistic on the interactive events that occurred in previous test executions,and focuses on the complicated relationship between components,which is more applicable to the component system regression testing.

Characterization of Stress Concentration by Tangential Component Hp(x) of Metal Magnetic Memory Signals

The correlation between the stress concentration and the spontaneous magnetic signals of metal magnetic memory(MMM) was investigated via tensile tests. Sheet specimens of the Q235 steel were machined into standard bars with rectangular holes to obtain various stress concentration factors. The tangential component Hp(x) of MMM signals and its related magnetic characteristic parameters throughout the loading process were presented and analyzed. It is found that the tangential component Hp(x) is sensitive to the abnormal magnetic changes caused by the local stress concentration in the defect area. The minimum magnetic field is positively correlated to the magnitude of the load and the distance from the notch. The tangential magnetic stress concentration factor presents good numerical stability during the entire loading process, and can be used to evaluate the stress concentration factor. The results obtained will be a complement to the MMM technique.

An Effective Long String Searching Algorithm towards Component Security Testing

In the execution of method invocation sequences to test component security,abnormal or normal information is generated and recorded in a monitor log. By searching abnormal information from monitor log,the exceptions that the component has can be determined. To facilitate the searching process,string searching methods could be employed. However,current approaches are not effective enough to search long pattern string. In order to mine the specific information with less number of matches,we proposed an improved Sunday string searching algorithm in this paper. Unlike Sunday algorithm which does not make use of the already matched characters,the proposed approach presents two ideas — utilizing and recycling these characters. We take advantage of all matched characters in main string,if they are still in the matchable interval compared with pattern string,to increase the distance that pattern string moves backwards. Experimental analysis shows that,compared to Sunday algorithm,our method could greatly reduce the matching times,if the scale of character set constituting both main string and pattern string is small,or if the length of pattern string is long. Also,the proposed approach can improve the search effectiveness for abnormal information in component security testing.

Seismic response of rocking isolated railway bridge piers with sacrificial components

In this study, sacrificial components were incorporated into self-centering railway bridge piers to improve the lateral stiffness. The seismic response of this new detail was investigated. First, the method to compute the initial uplift moment of the self-centering pier is given. In addition, shaking table tests were conducted on a free-rocking pier without sacrificial components, which was used to validate a two-spring numerical model. Good agreement was obtained between the numerical results and experimental data. Furthermore, the validated model was employed to investigate the influence of sacrificial components on the seismic response of rocking piers. For this purpose, two models were developed, with and without sacrificial components. Nonlinear response history analysis was then performed on both models under three historical motions. The results showed that compared to the one without sacrificial components, the rocking pier with sacrificial components has comparable displacement at the top of the pier, and maximum uplift moment at high amplitude motion. Therefore, incorporating sacrificial components into the rocking pier can increase the lateral stiffness at service load and low amplitude frequent earthquakes but can produce comparable response at high seismic excitation. These results provide support for performance-based seismic design of self-centering rocking piers.