目的:探讨用Th/Tr淋巴细胞比值评估乳腺癌患者的抗肿瘤免疫状态。方法:采用流式细胞术检测53例不同分期的乳腺癌患者化疗前外周血中CD4+Th细胞、CD4+CD25+Tr细胞水平,计算Th/Tr比值,并与30例健康志愿者进行比较。结果:有转移与无转移的...目的:探讨用Th/Tr淋巴细胞比值评估乳腺癌患者的抗肿瘤免疫状态。方法:采用流式细胞术检测53例不同分期的乳腺癌患者化疗前外周血中CD4+Th细胞、CD4+CD25+Tr细胞水平,计算Th/Tr比值,并与30例健康志愿者进行比较。结果:有转移与无转移的乳腺癌患者Tr细胞绝对值差异无统计学意义(103±75vs 109±70,P=0.722)。有转移的乳腺癌患者Th/Tr淋巴细胞比值小于无转移者(3.83±1.37 vs 6.11±2.93,P<0.001),无转移患者小于健康志愿者(6.11±2.93 vs 11.24±1.84,P<0.001)。结论:Th/Tr比值对乳腺癌患者抗肿瘤免疫状态的评估优于Tr细胞计数,Th/Tr比值在乳腺癌患者尤其是有转移的乳腺癌患者中显著降低,其水平变化可作为评估乳腺癌患者免疫状态的有效指标。展开更多
This study examined the effect of intensive insulin therapy on immune function and inflammatory factors at the early phase after severe trauma. At day 1, 3, 5, 7 after admission, subsets of CD4＋ helper T lymphocytes ...This study examined the effect of intensive insulin therapy on immune function and inflammatory factors at the early phase after severe trauma. At day 1, 3, 5, 7 after admission, subsets of CD4＋ helper T lymphocytes （Th1/Th2） and human leukocyte antigen （HLA）-DR expression on CD14＋ monocytes were flow cytometrically measured. Levels of cytokines, including tumor necrosis factor-alpha （TNF-α）, interleukin-6 （IL-6）, interleukin-10 （IL-10） and other immunity markers, such as IgA, IgG, IgM, C3, C4 and C reaction protein （CRP） were examined in two groups. The results showed that TNF-α, IL-6 and CRP levels in the intensive insulin therapy group were significantly lower than those in the conventional therapy group, whereas IL-10 levels were substantially increased after intensive insulin therapy. C3 level at day 3, 5, 7 and C4 levels at day 5, 7 were lower in the intensive therapy group than in the conventional therapy group. Th1/Th2 ratios decreased gradually over time in both groups, and were much lower at day 3, 5, 7 in intensive therapy group. There were significant differences among day 3 to day 7 after admission in HLA-DR expression in CD14＋ monocytes. It was concluded that the intensive insulin therapy could decrease pro-inflammatory cytokines and increase anti-inflammatory cytokines in the elderly suffering from severe trauma, at the same time, with complement recovery being delayed. Moreover, intensive insulin therapy promoted immune suppression and, therefore, measures need be taken to address the issue.展开更多
文摘目的:探讨用Th/Tr淋巴细胞比值评估乳腺癌患者的抗肿瘤免疫状态。方法:采用流式细胞术检测53例不同分期的乳腺癌患者化疗前外周血中CD4+Th细胞、CD4+CD25+Tr细胞水平,计算Th/Tr比值,并与30例健康志愿者进行比较。结果:有转移与无转移的乳腺癌患者Tr细胞绝对值差异无统计学意义(103±75vs 109±70,P=0.722)。有转移的乳腺癌患者Th/Tr淋巴细胞比值小于无转移者(3.83±1.37 vs 6.11±2.93,P<0.001),无转移患者小于健康志愿者(6.11±2.93 vs 11.24±1.84,P<0.001)。结论:Th/Tr比值对乳腺癌患者抗肿瘤免疫状态的评估优于Tr细胞计数,Th/Tr比值在乳腺癌患者尤其是有转移的乳腺癌患者中显著降低,其水平变化可作为评估乳腺癌患者免疫状态的有效指标。
基金supported by a grant from the National Natural Sciences Foundation of China(No.30700869)
文摘This study examined the effect of intensive insulin therapy on immune function and inflammatory factors at the early phase after severe trauma. At day 1, 3, 5, 7 after admission, subsets of CD4＋ helper T lymphocytes （Th1/Th2） and human leukocyte antigen （HLA）-DR expression on CD14＋ monocytes were flow cytometrically measured. Levels of cytokines, including tumor necrosis factor-alpha （TNF-α）, interleukin-6 （IL-6）, interleukin-10 （IL-10） and other immunity markers, such as IgA, IgG, IgM, C3, C4 and C reaction protein （CRP） were examined in two groups. The results showed that TNF-α, IL-6 and CRP levels in the intensive insulin therapy group were significantly lower than those in the conventional therapy group, whereas IL-10 levels were substantially increased after intensive insulin therapy. C3 level at day 3, 5, 7 and C4 levels at day 5, 7 were lower in the intensive therapy group than in the conventional therapy group. Th1/Th2 ratios decreased gradually over time in both groups, and were much lower at day 3, 5, 7 in intensive therapy group. There were significant differences among day 3 to day 7 after admission in HLA-DR expression in CD14＋ monocytes. It was concluded that the intensive insulin therapy could decrease pro-inflammatory cytokines and increase anti-inflammatory cytokines in the elderly suffering from severe trauma, at the same time, with complement recovery being delayed. Moreover, intensive insulin therapy promoted immune suppression and, therefore, measures need be taken to address the issue.
