Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cell...Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cells, and determined the population of Thl, Th2, Tcl and Tc2 of peripheral blood samples taken from 26 HCC patients before and after RFA. Results： The proportion of Typel cells （Thl and Tcl） and NK cells were significantly increased after RFA, especially in patients of the following subgroups： male, age〉55 years, pathological grade Ⅰ-Ⅱ tumor, clinical stage Ⅰ-Ⅱ or Child-Pugh A and B. Conclusion： TypeⅠ cells and NK cells in HCC patients were increased in a short period after RFA.展开更多
目的:本研究采用Meta分析方法,系统评估了COVID‐19患者外周血中TH1、TH2、TH17相关细胞因子水平,并深入探讨了这些细胞因子研究结果存在差异的原因。方法:本研究已通过国际荟萃分析INPLASY平台认证(INPLASY协议认证编号:202380023),通...目的:本研究采用Meta分析方法,系统评估了COVID‐19患者外周血中TH1、TH2、TH17相关细胞因子水平,并深入探讨了这些细胞因子研究结果存在差异的原因。方法:本研究已通过国际荟萃分析INPLASY平台认证(INPLASY协议认证编号:202380023),通过检索中国知网、PubMed、Embase、Cochrane和Web of Science数据库,收集COVID-19患者和健康对照群体外周血TH1、TH2、TH17相关细胞因子(TNF-a、IL-2、IL-4、IL-10、IL-17)水平的病例对照研究,检索时间范围为建库至2023年10月10日。由研究者独立完成文献筛选、数据提取和偏倚评估,使用RevMan 5.4软件进行Meta分析。结果:该Meta分析共检索出19,941项研究,最终纳入了6项进行Meta分析,这些研究均为中、高质量文献。患者入组的时间范围从2020年~2022年不等。结果表明:COVID-19感染组的患者外周血TNF-a水平显著高于健康对照组(HC组) [MD = 3.42, 95%CI (1.91, 4.92), I2 = 50%, P 2 = 98%, P = 0.0004];COVID-19感染组患者外周血IL-10水平显著高于健康对照组(HC组) [MD = 3.38, 95%CI (1.97, 4.80), I2 = 84%, P 2 = 98%, P = 0.009];COVID-19感染组患者与HC组外周血IL-2水平差异无统计学意义[MD = 0.89, 95%CI (−2.19, 3.97), I2 = 67%, P = 0.57]。结论:一些与TH1、TH2及TH17相关的细胞因子在COVID-19患者外周血中的水平明显高于健康对照组,这些细胞因子在新型冠状病毒感染的疾病进展中起着重要作用。Objective: In this study, we used Meta-analysis to systematically evaluate the levels of TH1, TH2, and TH17-related cytokines in the peripheral blood of COVID-19 patients, and the reasons for the differences in the findings of these cytokines were explored in depth. Methods: This study has been validated by the international Meta-analysis INPLASY platform (INPLASY protocol certification number: 202380023), by searching China Knowledge, PubMed, Embase, Cochrane and Web of Science databases, a case-control study on the levels of TH1, TH2, and TH17-related cytokines (TNFa, IL-2, IL-4, IL-10, and IL-17) in peripheral blood of COVID-19 patients and healthy control groups was collected, with a search timeframe from library construction to October 10, 2023. Literature screening, data extraction and bias assessment were done independently by the authors, and Meta-analysis was performed using RevMan 5.4 software. Results: The Meta-analysis retrieved a total of 19,941 studies, and 6 were finally included for Meta-analysis, which were all medium- and high-quality literature. The time of patient enrollment ranged from 2020 to 2022. The results showed that patients in the COVID-19-infected group had significantly higher peripheral blood TNF-a levels than in the healthy control group (HC group) [MD = 3.42, 95%CI (1.91, 4.92), I2 = 50%, P 2 = 98%, P = 0.0004];peripheral blood IL-10 levels were significantly higher in patients in the COVID-19-infected group than in the healthy control group (HC group) [MD = 3.38, 95%CI (1.97, 4.80), I2 = 84%, P 2 = 98%, P = 0.009];the difference in peripheral blood IL-2 levels between patients in the COVID-19-infected group and the HC group was not statistically significant [MD = 0.89, 95%CI (−2.19, 3.97), I2 =67%, P = 0.57]. Conclusion: Some cytokines related to TH1, TH2 and TH17 were significantly higher in the peripheral blood of COVID-19 patients than in the healthy control group, and these cytokines play an important role in the disease progression of novel coronavirus infections.展开更多
· AIM: To determine the effects of rapamycin on experimental autoimmune uveoretinitis(EAU) and investigate of role of rapamycin on T cell subsets in the disease.·METHODS: EAU was induced in rats using peptid...· AIM: To determine the effects of rapamycin on experimental autoimmune uveoretinitis(EAU) and investigate of role of rapamycin on T cell subsets in the disease.·METHODS: EAU was induced in rats using peptides1169 to 1191 of the interphotoreceptor binding protein(IRBP). Rapamycin(0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4 +CD25 + regulatory T cells(Tregs)from rat spleen were detected by flow cytometry.·RESULTS: Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, in vitro.Rapamycin also significantly increased TGF-β1production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore,rapamycin decreased the ratio of Th17 cells/CD4 +T cells and upregulated Tregs in EAU, as detected by flow cytometry.·CONCLUSION: Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU.Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.展开更多
文摘Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cells, and determined the population of Thl, Th2, Tcl and Tc2 of peripheral blood samples taken from 26 HCC patients before and after RFA. Results： The proportion of Typel cells （Thl and Tcl） and NK cells were significantly increased after RFA, especially in patients of the following subgroups： male, age〉55 years, pathological grade Ⅰ-Ⅱ tumor, clinical stage Ⅰ-Ⅱ or Child-Pugh A and B. Conclusion： TypeⅠ cells and NK cells in HCC patients were increased in a short period after RFA.
文摘目的:本研究采用Meta分析方法,系统评估了COVID‐19患者外周血中TH1、TH2、TH17相关细胞因子水平,并深入探讨了这些细胞因子研究结果存在差异的原因。方法:本研究已通过国际荟萃分析INPLASY平台认证(INPLASY协议认证编号:202380023),通过检索中国知网、PubMed、Embase、Cochrane和Web of Science数据库,收集COVID-19患者和健康对照群体外周血TH1、TH2、TH17相关细胞因子(TNF-a、IL-2、IL-4、IL-10、IL-17)水平的病例对照研究,检索时间范围为建库至2023年10月10日。由研究者独立完成文献筛选、数据提取和偏倚评估,使用RevMan 5.4软件进行Meta分析。结果:该Meta分析共检索出19,941项研究,最终纳入了6项进行Meta分析,这些研究均为中、高质量文献。患者入组的时间范围从2020年~2022年不等。结果表明:COVID-19感染组的患者外周血TNF-a水平显著高于健康对照组(HC组) [MD = 3.42, 95%CI (1.91, 4.92), I2 = 50%, P 2 = 98%, P = 0.0004];COVID-19感染组患者外周血IL-10水平显著高于健康对照组(HC组) [MD = 3.38, 95%CI (1.97, 4.80), I2 = 84%, P 2 = 98%, P = 0.009];COVID-19感染组患者与HC组外周血IL-2水平差异无统计学意义[MD = 0.89, 95%CI (−2.19, 3.97), I2 = 67%, P = 0.57]。结论:一些与TH1、TH2及TH17相关的细胞因子在COVID-19患者外周血中的水平明显高于健康对照组,这些细胞因子在新型冠状病毒感染的疾病进展中起着重要作用。Objective: In this study, we used Meta-analysis to systematically evaluate the levels of TH1, TH2, and TH17-related cytokines in the peripheral blood of COVID-19 patients, and the reasons for the differences in the findings of these cytokines were explored in depth. Methods: This study has been validated by the international Meta-analysis INPLASY platform (INPLASY protocol certification number: 202380023), by searching China Knowledge, PubMed, Embase, Cochrane and Web of Science databases, a case-control study on the levels of TH1, TH2, and TH17-related cytokines (TNFa, IL-2, IL-4, IL-10, and IL-17) in peripheral blood of COVID-19 patients and healthy control groups was collected, with a search timeframe from library construction to October 10, 2023. Literature screening, data extraction and bias assessment were done independently by the authors, and Meta-analysis was performed using RevMan 5.4 software. Results: The Meta-analysis retrieved a total of 19,941 studies, and 6 were finally included for Meta-analysis, which were all medium- and high-quality literature. The time of patient enrollment ranged from 2020 to 2022. The results showed that patients in the COVID-19-infected group had significantly higher peripheral blood TNF-a levels than in the healthy control group (HC group) [MD = 3.42, 95%CI (1.91, 4.92), I2 = 50%, P 2 = 98%, P = 0.0004];peripheral blood IL-10 levels were significantly higher in patients in the COVID-19-infected group than in the healthy control group (HC group) [MD = 3.38, 95%CI (1.97, 4.80), I2 = 84%, P 2 = 98%, P = 0.009];the difference in peripheral blood IL-2 levels between patients in the COVID-19-infected group and the HC group was not statistically significant [MD = 0.89, 95%CI (−2.19, 3.97), I2 =67%, P = 0.57]. Conclusion: Some cytokines related to TH1, TH2 and TH17 were significantly higher in the peripheral blood of COVID-19 patients than in the healthy control group, and these cytokines play an important role in the disease progression of novel coronavirus infections.
基金Supported by National Natural Science Foundation of China(No.81371005)
文摘· AIM: To determine the effects of rapamycin on experimental autoimmune uveoretinitis(EAU) and investigate of role of rapamycin on T cell subsets in the disease.·METHODS: EAU was induced in rats using peptides1169 to 1191 of the interphotoreceptor binding protein(IRBP). Rapamycin(0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4 +CD25 + regulatory T cells(Tregs)from rat spleen were detected by flow cytometry.·RESULTS: Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, in vitro.Rapamycin also significantly increased TGF-β1production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore,rapamycin decreased the ratio of Th17 cells/CD4 +T cells and upregulated Tregs in EAU, as detected by flow cytometry.·CONCLUSION: Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU.Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.
