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Myricetin alleviates ovalbumin-induced allergic rhinitis in mice by regulating Th1/Th2 balance 被引量:2
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作者 Ya-Nan Shi Jin-Zhu Su +1 位作者 Juan Wang Jiang-Qiao Geng 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2023年第7期306-314,共9页
Objective:To evaluate the effect of myricetin on ovalbumin(OVA)-induced allergic rhinitis in mice.Methods:Mice were sensitized and challenged using OVA(5%,500 mL)intraperitoneally and intranasally,respectively,on an a... Objective:To evaluate the effect of myricetin on ovalbumin(OVA)-induced allergic rhinitis in mice.Methods:Mice were sensitized and challenged using OVA(5%,500 mL)intraperitoneally and intranasally,respectively,on an alternative day for 14 days,followed by administration of myricetin(50,100,and 200 mg/kg)till day 21.Nasal symptoms,biochemical parameters,protein expressions,and histopathology were observed.Results:OVA-induced increased nasal symptoms including rubbing,sneezing,and discharge were significantly reduced by myricetin(100and 200 mg/kg)(P<0.05).Myricetin also protected against histamine challenge and attenuated elevated serum immunoglobulin E(IgE;total and OVA-specific),total IgG1,andβ-hexosaminidase levels,as well as leukotriene C4 and interleukins levels in nasal lavage fluid(P<0.05).Western blot analysis showed that myricetin significantly upregulated the protein expression of T-box expressed in T cells,while downregulating the protein expression of GATA binding protein 3,NF-κB,and IκB-α(P<0.05).Additionally,OVA-induced histopathological abberations in the nasal mucosa was markedly ameliorated by myricetin treatment(P<0.05).Conclusions:Myricetin exerts anti-allergic effects against OVAinduced allergic rhinitis via regulating Th1/Th2 balance. 展开更多
关键词 Allergic rhinitis GATA3 INTERLEUKINS MYRICETIN MONTELUKAST Ovalbumin th1/th2 balance
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Effect of glycyrrhetinic acid on Th1/Th2 balance in cough variant asthma mice
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作者 CHEN Qiu-ling ZHOU Bei +3 位作者 LI Yan-ling LIN Hui WU Yan-chun LIU Han 《Journal of Hainan Medical University》 CAS 2023年第16期1-6,共6页
Objective:To evaluate the therapeutic effect of Glycyrrhetinic Acid on cough variant asthma(CVA)mice and to investigate the possible mechanism in reducing lung inflammation.Methods:48 young female Balb/c mice were div... Objective:To evaluate the therapeutic effect of Glycyrrhetinic Acid on cough variant asthma(CVA)mice and to investigate the possible mechanism in reducing lung inflammation.Methods:48 young female Balb/c mice were divided into Control,CVA,Prednisone Acetate,Glycyrrhetinic Acid high-dose,Glycyrrhetinic Acid middle-dose and Glycyrrhetinic Acid lowdose groups randomly,with 8 mice in each group.The CVA mice model was established by ovalbumin(OVA)sensitization and OVA challenge,the animal asthma behavior was observed after drug administration,and the index of the lung of mice were recorded.The level of OVAsIgE in the bronchoalveolar lavage fluid(BALF)was tested by ELISA.The pathological changes of the lung tissue were observed by Hematoxylin and Eosin(H&E)staining.The protein expressions of T-bet,IFN-γ,Gata3,IL-4 and IL-13 in the lung tissue were determined by Western blot.Results:Compared with the CVA group,the index of lung of mice,the OVA-sIgE level in BALF and expression levels of Th2-related factor in the lung tissue of mice in Prednisone Acetate and Glycyrrhetinic Acid groups were significantly decreased(P<0.05 or P<0.01),the infiltration of inflammatory cells in the lung tissue was reduced,while expressions of Th1-related factor in the lung tissue was significantly increased(P<0.05 or P<0.01).Conclusion:Glycyrrhetinic acid has therapeutic effect on CVA mice,the underlying mechanism of Glycyrrhetinic acid alleviating lung impairment and airway inflammation may be associated with mediating the Th1/Th2 imbalance in the lung tissue. 展开更多
关键词 Glycyrrhetinic acid Cough variant asthma(CVA) th1/th2 balance
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All-transRetinoic Acid Regulates Th1/Th2 Balance in CD4+T cells When GATA-3 is Deficient 被引量:6
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作者 ZHU Yan Feng HU Jia Zhe +2 位作者 ZHAO Pin Nan LIU Lin Xi and LI Yun 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第9期774-777,共4页
The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balanc... The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balance under a strong Thl-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite allotrans retinoic acid (ATRA) on ThloTh2 differentiation in CD4~ T cells under GATA-3 deficiency, which can induce Thl-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA. 展开更多
关键词 GATA cell th All-transRetinoic Acid Regulates th1/th2 balance in CD4+T cells When GATA-3 is Deficient CD
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Effect of Th1/Th2 balance on serum prostaglandin,COX-2,AVP and OT in primary dysmenorrhea rats with cold coagulation and blood stasis syndrome
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作者 Feng-Ting Zhai Dong-Mei Wang +3 位作者 Qing Ma Wei-Ping Cao Dan-Dan Wang Guang-Wen Li 《Journal of Hainan Medical University》 2021年第15期1-5,共5页
Objective:Explore the role of Th1/Th2 balance in the pathogenesis of primary dysmenorrhea through study the effect of Th1/Th2 balance drift on serum prostaglandin F_(2α),prostaglandin,6-keto-prostaglandin F_(1α),TXB... Objective:Explore the role of Th1/Th2 balance in the pathogenesis of primary dysmenorrhea through study the effect of Th1/Th2 balance drift on serum prostaglandin F_(2α),prostaglandin,6-keto-prostaglandin F_(1α),TXB2,COX-2,AVP and OT in Primary Dysmenorrhea Rats with cold coagulation and blood stasis syndrome.Method:The rat model of primary dysmenorrhea with cold coagulation and blood stasis syndrome was established.On the basis of this model,Th1 shift model and Th2 shift model were established.The rats were divided into blank group,model group,Th1 shifting group and Th2 shifting group.The writhing reaction of rats in each group was compared,and the contents of PGF_(2α),PGE_(2),6-keto-PGF_(1α),TXB2,COX-2,AVP and OT in serum were detected.Results:Writhing latency:There was no significant difference between Th1 and Th2 shifting groups(P>0.05),Th2 shifting group was shorter than Th1 shifting group(P<0.05).Writhing times in 30 minutes:Compared with the model group,the writhing times of Th1 shifting group decreased significantly(P<0.01),while Th2 shifting group increased significantly(P<0.01).Compared with the blank group,the contents of PGF_(2α),TXB2,COX-2,AVP and OT were increased(P<0.05,P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2)were decreased(P<0.01).Compared with the model group,the contents of PGF_(2α),TXB2,COX-2 and AVP in Th1 shifting group were decreased(P<0.05,P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2)were increased(P<0.01),while the contents of PGF_(2α),TXB2,COX-2,AVP and OT in Th2 shifting group were increased(P<0.01),and the contents of 6-keto-PGF_(1α)and PGE_(2)were decreased(P<0.01).Conclusion:The intervention of Th1/Th2 cell balance on the expression of PGF_(2α),PGE_(2),6-keto-PGF_(1α),TXB2,COX-2,AVP and OT may be one of the pathogenesis of PD with cold coagulation and blood stasis syndrome. 展开更多
关键词 Primary dysmenorrhea th1/th2 balance PROSTAGLANDIN CYCLOOXYGENASE-2 VASOPRESSIN OXYTOCIN
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浅谈复方中草药制剂对Th1-Th2细胞平衡的影响
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作者 曹文斌 王文魁 +1 位作者 顾宪红 韩克光 《河南畜牧兽医》 2007年第03S期13-14,共2页
结合Th1-Th2细胞平衡的理论,中医药领域开展了相关的试验研究,并已取得了一定的成果。本文在综述前人研究成果基础上,提出了加强中草药免疫药理学机理研究的必要性,中草药复方制剂对免疫平衡影响的研究可能是开发出困扰养殖业持续快速... 结合Th1-Th2细胞平衡的理论,中医药领域开展了相关的试验研究,并已取得了一定的成果。本文在综述前人研究成果基础上,提出了加强中草药免疫药理学机理研究的必要性,中草药复方制剂对免疫平衡影响的研究可能是开发出困扰养殖业持续快速发展疾病的中草药复方制剂的突破口。 展开更多
关键词 复方 中草药 th1-th2 平衡 影响
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Scutellarin alleviates complete freund’s adjuvant-induced rheumatoid arthritis in mice by regulating the Keap1/Nrf2/HO-1 pathway
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作者 JIAN LI QINGQING WANG XIAOYING ZHANG 《BIOCELL》 SCIE 2023年第6期1307-1316,共10页
Scutellarin(SCU)is a herbal flavonoid glucuronide with multiple pharmacological activities,including antioxidant,anti-inflammation,vascular relaxation,anti-platelet,and myocardial protection.However,the effect of SCU... Scutellarin(SCU)is a herbal flavonoid glucuronide with multiple pharmacological activities,including antioxidant,anti-inflammation,vascular relaxation,anti-platelet,and myocardial protection.However,the effect of SCU on complete Freund’s adjuvant(CFA)-induced rheumatoid arthritis(RA)had not been studied.In this study,we investigated the beneficial effects of SCU in the CFA-induced RA mice model and the anti-arthritic activity was evaluated by paw edema.Enzyme-linked immunosorbent assay(ELISA)was carried out to evaluate the plasma levels of immunoglobulin(Ig)G,IgE,tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,receptor activator of nuclear factor-κB ligand(RANKL),and osteoprotegerin(OPG).Histological slides were prepared from the harvested paws of mice to determine the pathological changes in the joints.The proportions of T helper type 1(Th1)and T helper type 2(Th2)cells of CD4+T lymphocyte subsets were analyzed by flow cytometry.The expression of Kelch-like ECHassociated protein 1(Keap1),nuclear factor erythroid 2-related factor 2(Nrf2),and heme oxygenase-1(HO-1)was analyzed using real-time quantitative PCR(RT-qPCR)and western blotting assays.The present study demonstrated that SCU prevented CFA-induced RA,and inhibited the expression of inflammation factors,IgG,IgE,TNF-α,IL-1β,and IL-6.While SCU also reduced the RANKL level,it increased OPG expression in RA mice.The Th1/Th2 ratio was significantly lower in mice treated with SCU.Additionally,HO-1 expression was reduced while the expression of Keap1 and Nrf2 was elevated following SCU treatment.Results provide preliminary evidence to employ SCU in arthritis treatment which might be related to the regulation of Th1/Th2 balance and the Keap1/Nrf2/HO-1 pathway. 展开更多
关键词 SCUTELLARIN Rheumatoid arthritis th1/th2 balance Keap1/Nrf2/HO-1 pathway Immunosuppression
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Th1-Th2平衡失调与人类疾病关系及其相关治疗研究现状 被引量:21
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作者 刘加洪 王英年 +2 位作者 赵云峰 姚艳红 张克佳 《青岛大学医学院学报》 CAS 2002年第4期366-368,共3页
关键词 th1-th2平衡失调 人类疾病 免疫病理学 淋巴细胞转化
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RAI1脱敏干预影响变应性鼻炎大鼠模型ECP表达和Th1-Th2分化的机制 被引量:4
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作者 杨守云 肖艳 《中国医药生物技术》 2019年第6期540-544,共5页
目的探究探针RAI1脱敏干预影响变应性鼻炎大鼠模型ECP表达和Th1-Th2分化的机制方法卵白蛋白(OVA)诱导雌性Sprague-dawley大鼠构建过敏性鼻炎(AR)模型。并进行构建沉默RAI1 mRNA质粒载体,脑内注射至AR模型大鼠体内。通过蛋白质印迹法检测... 目的探究探针RAI1脱敏干预影响变应性鼻炎大鼠模型ECP表达和Th1-Th2分化的机制方法卵白蛋白(OVA)诱导雌性Sprague-dawley大鼠构建过敏性鼻炎(AR)模型。并进行构建沉默RAI1 mRNA质粒载体,脑内注射至AR模型大鼠体内。通过蛋白质印迹法检测Th1-Th2相关因子的蛋白表达情况;通过qRT-PCR分析各组大鼠中维甲酸诱导蛋白RAI1的mRNA表达情况;通过记录大鼠鼻炎行为评估变应性鼻炎症状;通过酶联免疫吸附试验检测各组大鼠血清中ECP、IgE含量。结果大鼠搔鼻挠嘴、打喷嚏行为AR模型组较对照组、RAI1干预组频繁(P<0.05),说明AR模型建立成功。RAI1干预组(1.02±0.01)较AR模型组(2.85±0.25)、对照组(2.69±0.11)相比,RAI1 mRNA表达降低(P<0.05),说明病毒载体构建成功,RAI1蛋白表达受到抑制。Th1相关因子IL-2、IFN-γ的蛋白表达AR模型组较对照组、RAI1干预组低(P<0.05)。Th2相关因子IL-4、IL-5的蛋白表达AR模型组较对照组、RAI1干预组高(P<0.05)。经酶联免疫吸附检测ECP、IgE含量,AR模型组(3.11±0.23、29.51±3.64)较对照组(1.62±0.18、13.72±2.68)、RAI1干预组(1.85±0.14、17.66±2.16)升高(P<0.05)。结论RAI1脱敏干预会降低ECP蛋白表达,平衡Th1-Th2生物功能,对变应性鼻炎具有治疗效果,减轻鼻炎症状。 展开更多
关键词 鼻炎 变应性 季节性 脱敏法 免疫 th1-th2平衡 嗜酸性粒细胞阳离子蛋白 炎症反应 RAI1
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淫羊藿总黄酮(EF)对老龄大鼠Th1、Th2、Th3细胞的调节作用 被引量:15
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作者 刘小雨 沈自尹 +1 位作者 王琦 陈伟华 《中国免疫学杂志》 CAS CSCD 北大核心 2005年第11期842-845,849,共5页
目的:探讨淫羊藿总黄酮(EF)对衰老大鼠脾淋巴细胞Th1、Th2、Th3各类细胞因子的调节作用。方法:采用流式细胞仪,监测比较老龄大鼠脾脏淋巴细胞凋亡率;采用Th1、Th2、Th3 cDNA芯片,分别检测正常青年组(YC)、老龄大鼠组(OM)、EF组、NF-KB... 目的:探讨淫羊藿总黄酮(EF)对衰老大鼠脾淋巴细胞Th1、Th2、Th3各类细胞因子的调节作用。方法:采用流式细胞仪,监测比较老龄大鼠脾脏淋巴细胞凋亡率;采用Th1、Th2、Th3 cDNA芯片,分别检测正常青年组(YC)、老龄大鼠组(OM)、EF组、NF-KB阻断剂组(PDTC)、PDTC+EF组的Th1、Th2、Th3,各类细胞因子信号差异表达。结果:①老龄大鼠普遍存在淋巴细胞过度凋亡现象,EF可显著抑制这种过度凋亡状态。②YC组,Th1、Th2、Th3类细胞因子之间处于平衡状态(P>0.05);OM组Th1、Th2、Th3类细胞因子表达比YC组增高,呈Th2、Th3类细胞因子优势应答(P<0.05)。③EF组Th1、Th2、Th3类细胞因子均较OM组明显降低(P<0.05),其趋势与YC组近似,并且Th1、Th2、Th3类细胞恢复平衡。④NFKB通路抑制后(PDTC组),Th1、Th2、Th3类细胞因子平衡失调,Th2优势更加明显(P<0.05);而PDTC+EF组,Th1、Th2、Th3各组表达值则较PDTC组下调,其组内Th1和Th2也重新达到一种相对平衡状态。结论:老龄大鼠脾淋巴细胞过度凋亡,Th1、Th2、Th3类细胞因子分泌增高,Th2、Th3类细胞因子优势应答;EF可以抑制淋巴细胞过度凋亡,校正老龄大鼠Th1、Th2、Th3类细胞因子之间的比例失衡,重塑Th1-Th2-Th3细胞免疫调节网络的良性平衡。 展开更多
关键词 淫羊藿总黄酮 th1-th2-th3 细胞因子 基因芯片 细胞凋亡
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支气管哮喘患者外周血Th1、Th2与Th17细胞表达水平及临床意义 被引量:84
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作者 高阳 杨帆 《解放军医药杂志》 CAS 2017年第1期76-79,共4页
目的探讨外周血Th1、Th2与Th17细胞在支气管哮喘患者中的表达水平及临床意义。方法选取2015年1月—2016年2月诊治的支气管哮喘40例,均为急性发作期,治疗1周后为缓解期,选取同期健康人40例作为对照组。检测支气管哮喘急性发作期、缓解期... 目的探讨外周血Th1、Th2与Th17细胞在支气管哮喘患者中的表达水平及临床意义。方法选取2015年1月—2016年2月诊治的支气管哮喘40例,均为急性发作期,治疗1周后为缓解期,选取同期健康人40例作为对照组。检测支气管哮喘急性发作期、缓解期和对照组外周血Th1、Th2与Th17细胞水平,观察外周血中干扰素(IFN)-γ、白介素(IL)-4及IL-17的表达情况。结果支气管哮喘组急性发作期和缓解期Th1、Th1/Th2、IFN-γ的表达水平低于对照组,且急性发作期低于缓解期(P<0.05);支气管哮喘组急性发作期和缓解期Th2、Th17、IL-4、IL-17表达水平高于对照组,且急性发作期高于缓解期(P<0.05)。结论支气管哮喘患者外周血Th1、Th2与Th17细胞表达水平失衡,导致IFN-γ、IL-4及IL-17异常分泌,可能参与支气管哮喘的发病。 展开更多
关键词 哮喘 th1-th2平衡 th17细胞
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紫草素对特应性皮炎小鼠TSLP/OX40L通路及Th1/Th2平衡的影响 被引量:11
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作者 吴金环 郑宝勇 张理涛 《天津医药》 CAS 北大核心 2021年第9期949-954,共6页
目的探究紫草素对特应性皮炎(AD)小鼠胸腺基质淋巴细胞生成素(TSLP)/OX40配体(OX40L)通路及辅助性T细胞(Th)1/Th2平衡的影响。方法采用局部外涂2,4-二硝基氟苯(DNFB)法制备AD小鼠模型。采用随机数字表法将模型小鼠分为模型组,紫草素低(2... 目的探究紫草素对特应性皮炎(AD)小鼠胸腺基质淋巴细胞生成素(TSLP)/OX40配体(OX40L)通路及辅助性T细胞(Th)1/Th2平衡的影响。方法采用局部外涂2,4-二硝基氟苯(DNFB)法制备AD小鼠模型。采用随机数字表法将模型小鼠分为模型组,紫草素低(20 mg/kg)、中(30 mg/kg)、高(40 mg/kg)剂量组和阳性对照组(泼尼松龙,10 mg/kg),每组15只;另取15只作为正常对照组。各给药组大鼠按10 mL/kg灌胃相应药物,模型组和正常对照组灌胃等体积溶剂,每日1次,连续15 d。分别于给药后第5、10和15天观察小鼠搔抓行为;于给药后第7、15天评价小鼠皮损状况;末次给药结束后,HE染色观察小鼠皮损组织病理学变化;流式细胞术检测小鼠外周血中Th1/Th2比例;酶联免疫吸附试验(ELISA)检测小鼠血清中TSLP、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-4和干扰素(IFN)-γ水平;Western blot法检测小鼠皮损组织TSLP和OX40L蛋白表达水平。结果与正常对照组相比,模型组小鼠搔抓行为增加,皮肤炎性损伤加重;皮损组织中表皮和真皮区域均有密集的炎性细胞浸润,表皮增厚;外周血Th2细胞、TSLP、TNF-α和IL-4水平以及皮损组织TSLP和OX40L蛋白表达水平显著升高(P<0.05),外周血Th1细胞、Th1/Th2比例和IFN-γ水平显著下降(P<0.05)。与模型组相比,紫草素低、中、高剂量组小鼠第5、10、15天的搔抓行为减少,第7、15天皮肤炎性损伤依次减轻(P<0.05);小鼠表皮和真皮区域炎性细胞浸润和表皮增厚依次减轻,外周血Th2细胞、TSLP、TNF-α和IL-4水平以及皮损组织TSLP和OX40L蛋白表达水平依次降低(P<0.05),Th1细胞、Th1/Th2比例和IFN-γ水平依次升高(P<0.05)。结论紫草素可能通过抑制TSLP/OX40L通路维持AD小鼠外周血中Th1/Th2平衡,改善皮肤炎性损伤。 展开更多
关键词 紫草素 皮炎 特应性 th1-th2平衡 OX40配体 胸腺基质淋巴细胞生成素/OX40L通路
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肝细胞癌患者肝动脉化疗栓塞术后外周血长链非编码RNA TINCR水平与Th1/Th2平衡及预后的相关性分析 被引量:7
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作者 柏涛 王静丽 +3 位作者 胡旭东 夏冰 程海林 王娟 《临床肝胆病杂志》 CAS 北大核心 2021年第1期79-83,共5页
目的探讨分析外周血长链非编码RNA(lncRNA)TINCR表达水平与肝细胞癌(HCC)患者行肝动脉化疗栓塞术(TACE)术后辅助性T淋巴细胞1(Th1)/Th2平衡及预后的相关性。方法回顾性选取2015年3月—2017年3月在湖北省武汉市金银潭医院行TACE治疗的HC... 目的探讨分析外周血长链非编码RNA(lncRNA)TINCR表达水平与肝细胞癌(HCC)患者行肝动脉化疗栓塞术(TACE)术后辅助性T淋巴细胞1(Th1)/Th2平衡及预后的相关性。方法回顾性选取2015年3月—2017年3月在湖北省武汉市金银潭医院行TACE治疗的HCC患者85例,并随机抽取在本院体检的健康志愿者30例作为对照组。检测HCC患者TACE完成后7 d及对照组外周血lncRNA TINCR表达情况、Th1/Th2细胞因子表达情况。计量资料组间比较采用t检验,计数资料组间比较采用χ2检验,等级资料组间比较采用Kruskal-Wallis H检验。lncRNA TINCR相对表达量与Th1/Th2细胞因子的相关性采用Spearman相关性分析。lncRNA TINCR相对表达量对生存情况的影响采用Kaplan-Meier生存曲线分析,生存曲线比较采用log-rank检验。结果HCC组患者外周血lncRN TINCR相对表达量(1.784±0.429)高于对照组(0.926±0.263),差异有统计学意义(t=10.277,P<0.05)。lncRNA TINCR相对表达与HCC患者血管侵犯、肿瘤大小、肿瘤分期及血清AFP水平有关(t值分别为3.958、4.499、3.361、4.949,P值分别为<0.001、<0.001、0.001、<0.001)。HCC组患者血清IFNγ、IL-2水平明显低于对照组[(13.48±5.20)pg/ml vs(27.49±7.21)pg/ml、(21.89±6.45)pg/ml vs(32.05±7.89)pg/ml,t值分别为11.408、6.986,P值均<0.05],而IL-4、IL-10水平明显高于对照组[(13.73±3.35)pg/ml vs(9.36±2.73)pg/ml、(12.75±2.74)pg/ml vs(8.93±2.16)pg/ml,t值分别为6.426、6.909,P值均<0.05]。HCC患者外周血lncRNA TINCR相对表达量与血清IFNγ、IL-2水平呈负相关(r值分别为-3.164、-3.270,P值均<0.001),与患者血清IL-4、IL-10呈正相关(r值分别为2.963、3.044,P值分别为0.007、<0.001)。以lncRNA TINCR相对表达量≥1.700作为高表达,85例HCC患者中lncRNA TINCR高表达患者47例,低表达患者38例。log-rank检验结果显示,lncRNA TINCR低表达患者生存情况明显优于高表达患者(χ2=4.182,P<0.05)。结论HCC患者行TACE后外周血lncRNA TINCR表达明显高于健康人群,lncRNA TINCR表达与患者病理特征及Th1/Th2免疫平衡有密切联系,可作为患者生存预后的潜在预测指标之一。 展开更多
关键词 肝细胞 化学栓塞 治疗性 RNA 长链非编码 th1-th2平衡 预后
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新生儿低氧缺血性脑病Th_2细胞功能的检测 被引量:1
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作者 张秋业 董延妮 +1 位作者 董增义 姜红 《青岛大学医学院学报》 CAS 2002年第3期209-210,219,共3页
①目的 了解低氧缺血性脑病 (HIE)新生儿Th2 细胞功能变化及其与HIE间的关系。②方法 采用ELISA法和单克隆抗体间接免疫荧光法 ,检测 4 6例HIE病儿不同病期血浆和外周血单个核细胞 (PBMC)体外产生白细胞介素 (IL) 6 ,IL 8,IL 10水平... ①目的 了解低氧缺血性脑病 (HIE)新生儿Th2 细胞功能变化及其与HIE间的关系。②方法 采用ELISA法和单克隆抗体间接免疫荧光法 ,检测 4 6例HIE病儿不同病期血浆和外周血单个核细胞 (PBMC)体外产生白细胞介素 (IL) 6 ,IL 8,IL 10水平和T细胞IL 2受体 (IL 2R)表达率 ,并与正常足月新生儿脐血检测结果进行对照。③结果 HIE病程第 1天 (D1)血浆及PBMC体外产生IL 6 ,IL 8和IL 10水平明显高于正常对照组 (z =4 .6 5 3~ 5 .85 6 ,P <0 .0 1) ;中重度及并发多器官功能障碍综合征 (MODS)的HIE病儿血浆及PBMC体外产生IL 6 ,IL 8水平分别高于轻度及无MODS病儿 (z =2 .2 2 8~ 2 .70 1,P <0 .0 5 ;z =2 .877~ 4 .187,P <0 .0 1) ,而IL 10水平无显著性差异 (z =0 .4 5 0~ 1.85 0 ,P >0 .0 5 ) ;T细胞IL 2R表达率明显低于正常对照组 (t=3.5 6 1,P <0 .0 1) ,中重度HIE病儿尤著 (t′ =4 .194 ,P <0 .0 1)。血浆IL 6 ,IL 8,IL 10水平间呈显著正相关 (r =0 .6 2 9~ 0 .74 6 ,P <0 .0 1) ;血浆及PBMC体外产生IL 6 ,IL 8水平与血清脑型磷酸激酶水平呈显著正相关 (r =0 .4 89~ 0 .6 91,P <0 .0 5 ,0 .0 1) ,与T细胞IL 2R表达率呈显著负相关 (r=- 0 .394~ - 0 .4 5 1,P <0 .0 5 ,0 .0 1)。病程第 3天 (D3) 展开更多
关键词 白细胞介素-4 干扰素Ⅱ型 th1-th2平衡 胎血
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敲除H2-eb1基因建立的变应性鼻炎模型小鼠
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作者 李林格 冯娟 +6 位作者 胡斌 寿玺 张春 田钰 江春荣 张瑜 张华 《中国组织工程研究》 CAS 北大核心 2015年第27期4417-4422,共6页
背景:HLA-DRB1与变应性鼻炎发病发病有关,构建HLA-DRB1基因敲除动物模型,不仅为阐明变应性鼻炎发病机制、同时也为相关疾病发病机制的阐明提供了良好的途径,然而未查阅到利用H2-eb1基因敲除小鼠进行相关研究的报道。目的:构建HLA-DRB1... 背景:HLA-DRB1与变应性鼻炎发病发病有关,构建HLA-DRB1基因敲除动物模型,不仅为阐明变应性鼻炎发病机制、同时也为相关疾病发病机制的阐明提供了良好的途径,然而未查阅到利用H2-eb1基因敲除小鼠进行相关研究的报道。目的:构建HLA-DRB1基因敲除动物模型。方法:经杂合子小鼠近亲繁殖,获得纯合子、野生型和杂合子小鼠。经基因及蛋白鉴定确认,采用随机数字表法选取8周龄雌性野生型(H2-eb1+/+)小鼠12只和H2-eb1-/-小鼠12只,将12只H2-eb1+/+小鼠和12只H2-eb1-/-小鼠以卵清蛋白致敏激发,建立小鼠变应性鼻炎模型。将另12只H2-eb1+/+小鼠以PBS代替卵清蛋白激发作为对照。结果与结论:与对照小鼠相比,变应性鼻炎模型小鼠血清中卵清蛋白IgE、白细胞介素4水平明显升高,γ-干扰素水平明显降低;而与基因敲除野生型小鼠(H2-eb1+/+)相比,基因敲除H2-eb1-/-变应性鼻炎小鼠IgE、白细胞介素4水平较低,γ-干扰素水平较高。提示H2-eb1基因在变应性鼻炎的发病机制中的Th1/Th2失衡有重要调节作用。 展开更多
关键词 鼻炎 变应性 常年性 基因敲除技术 近亲繁殖 th1-th2平衡 实验动物 基因病毒载体及相关因子动物模型 变态反应性疾病 变应性鼻炎 基因敲除 基因型聚合酶链反应 免疫印迹 th1/th2平衡 国家自然科学基金
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扶正抗癌方联合调强放疗干预老年性食管癌的临床疗效及对辅助性T细胞1、辅助性T细胞2细胞因子的影响 被引量:1
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作者 徐超 周雷 +1 位作者 王小龙 孙波 《河北中医》 2019年第12期1849-1853,共5页
目的观察扶正抗癌方联合调强放疗干预老年性食管癌的临床疗效及对辅助性T细胞(Th)1、Th2细胞因子的影响。方法将76例老年性食管癌患者按照随机数字表法分为2组。对照组38例予调强放疗;治疗组38例在对照组治疗基础上加用扶正抗癌方口服,... 目的观察扶正抗癌方联合调强放疗干预老年性食管癌的临床疗效及对辅助性T细胞(Th)1、Th2细胞因子的影响。方法将76例老年性食管癌患者按照随机数字表法分为2组。对照组38例予调强放疗;治疗组38例在对照组治疗基础上加用扶正抗癌方口服,疗程6周。评价2组近期疗效,比较2组治疗前后血清肿瘤标志物[糖类抗原125(CA125)、糖类抗原19-9(CA19-9)、癌胚抗原(CEA)、鳞状上皮细胞癌抗原(SCC-Ag)]、Th1和Th2细胞因子[白细胞介素2(IL-2)、干扰素γ(IFN-γ)、IL-4、IL-10]水平变化,记录2组毒副作用发生情况。结果治疗组近期有效率78.95%,对照组近期有效率60.53%,治疗组近期疗效优于对照组(P<0.05)。2组治疗后血清肿瘤标志物CA125、CA19-9、CEA、SCC-Ag水平均降低(P<0.05),且治疗组治疗后CA125、CA19-9、CEA、SCC-Ag水平均低于对照组(P<0.05)。治疗组治疗后IL-2、IFN-γ水平升高(P<0.05),IL-4、IL-10水平降低(P<0.05);对照组治疗后IL-2、IFN-γ水平降低(P<0.05),IL-4、IL-10水平升高(P<0.05)。治疗组治疗后Th1/Th2细胞因子免疫失衡状态改善优于对照组(P<0.05)。治疗组白细胞计数减少、血小板计数减少、呕吐、放射性肺炎、放射性食管炎、肝肾功能损害发生率均低于对照组(P<0.05)。结论扶正抗癌方联合调强放疗干预老年性食管癌患者的抗癌疗效确切,利于降低肿瘤标志物的水平,纠正Th1/Th2细胞因子免疫失衡状态,毒副作用发生率较低,值得临床推广。 展开更多
关键词 食管肿瘤 放射疗法 扶正 中西医结合疗法 th1-th2平衡
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Faecalibacterium prausnitzii基因组DNA上调外周血单个核细胞Th1型免疫应答增强对人结肠癌LoVo细胞的杀伤活性
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作者 张涛 张敏 +3 位作者 汤爱荣 曹萍 谢丽娟 于成功 《胃肠病学》 2015年第8期457-461,共5页
背景:Faecalibacterium prausnitzii(Fp)是一种具有抗炎和免疫调节作用的肠道共生菌,研究发现结直肠癌患者肠道内Fp数量明显减少,可能与肿瘤发生有关。目的:探讨Fp及其基因组DNA(fD NA)干预对外周血单个核细胞(PBMCs)对人结肠癌LoV o细... 背景:Faecalibacterium prausnitzii(Fp)是一种具有抗炎和免疫调节作用的肠道共生菌,研究发现结直肠癌患者肠道内Fp数量明显减少,可能与肿瘤发生有关。目的:探讨Fp及其基因组DNA(fD NA)干预对外周血单个核细胞(PBMCs)对人结肠癌LoV o细胞杀伤活性的影响及其免疫学机制。方法:将Fp、fD NA或酶解fD NA(d-fD NA)与分离自健康成人的PBMCs体外共培养,MTT实验检测PBMCs对LoV o细胞的杀伤活性,ELISA法检测PBMCs培养上清液中的Th1、Th2型细胞因子干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)含量,real-time PCR检测PBMCs Th1、Th2特异性转录因子T-bet、GATA3表达。结果:与未予干预的PBMCs相比,fD NA干预能显著增强PBMCs对LoV o细胞的杀伤活性(P<0.05),同时促进PBMCs的IFN-γ分泌和T-bet mRNA表达(P<0.05),抑制IL-4分泌和GATA3mRNA表达(P<0.05)。Fp和d-fD NA均未显示出上述作用。结论:fD NA能增强PBMCs对人结肠癌细胞的杀伤活性,其机制可能与上调Th1型免疫应答有关。 展开更多
关键词 Faecalibacterium prausnitzii 干扰素Γ 白细胞介素4 th1-th2平衡 结肠肿瘤 LOVO细胞
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灭荨汤联合窄谱中波紫外线照射对血虚风燥型慢性荨麻疹患者皮损症状、血液流变学及辅助性T细胞1/2平衡的影响 被引量:6
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作者 任谦 何晓晓 刘晓晨 《河北中医》 2021年第2期291-295,共5页
目的观察灭荨汤联合窄谱中波紫外线照射对血虚风燥型慢性荨麻疹患者皮损症状、血液流变学及辅助性T细胞1/辅助性T细胞2(Th1/Th2)平衡的影响。方法将112例血虚风燥型慢性荨麻疹患者按照随机数字表法分为2组。对照组56例予窄谱中波紫外线... 目的观察灭荨汤联合窄谱中波紫外线照射对血虚风燥型慢性荨麻疹患者皮损症状、血液流变学及辅助性T细胞1/辅助性T细胞2(Th1/Th2)平衡的影响。方法将112例血虚风燥型慢性荨麻疹患者按照随机数字表法分为2组。对照组56例予窄谱中波紫外线照射,治疗组56例在对照组治疗基础上联合灭荨汤治疗。2组均治疗1个月。比较2组治疗前及治疗2周、1个月症状与体征评分、血液流变学指标[全血黏度(高切)(HSV)、全血黏度(低切)(LSV)、红细胞沉降率(ESR)、纤维蛋白原(FIB)]、血清Th1/Th2细胞因子[白细胞介素10(IL-10)、IL-18、IL-4、干扰素γ(IFN-γ)]水平,并统计疗效及不良反应发生情况。结果治疗组总有效率92.86%(52/56),对照组总有效率76.79%(43/56),治疗组疗效优于对照组(P<0.05)。治疗2周、1个月2组各症状、体征评分及总分均较本组治疗前降低(P<0.05),治疗1个月均较本组治疗2周降低(P<0.05),且治疗2周、1个月治疗组均低于对照组同期(P<0.05)。治疗2周、1个月后治疗组HSV、LSV、ESR、FIB水平均较本组治疗前降低(P<0.05),治疗1个月均较本组治疗2周降低(P<0.05),且治疗2周、1个月治疗组均低于对照组同期(P<0.05)。治疗2周、1个月2组血清IL-10、IL-4水平均较本组治疗前降低(P<0.05),IL-18、IFN-γ水平均升高(P<0.05),治疗1个月2组血清IL-10、IL-4水平均较本组治疗2周降低(P<0.05),IL-18、IFN-γ水平均较本组治疗2周升高(P<0.05),且治疗2周、1个月治疗组血清IL-10、IL-4水平均低于对照组同期(P<0.05),IL-18、IFN-γ水平均高于对照组同期(P<0.05)。2组不良反应发生率比较差异无统计学意义(P>0.05)。结论灭荨汤联合窄谱中波紫外线照射治疗血虚风燥型慢性荨麻疹可减轻皮损及瘙痒症状,改善血液流变学,调节Th1/Th2细胞平衡,提高疗效,且安全性好。 展开更多
关键词 荨麻疹 紫外线疗法 中西医结合疗法 血液流变学 th1-th2平衡
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Hydrolyzed seawater pearl tablet modulates the immunity via attenuating Th1/Th2 imbalance in an immunosuppressed mouse model 被引量:9
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作者 LAN Taijin LUO Xuefei +10 位作者 MO Mingyue CHEN Zhenxing LUO Fei HAN Siyin LIU Peng LIANG Zhongxiu ZHANG Ting LI Tianyi YAN Qiangqiang LIN Yong LIN Jiang 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2021年第3期397-405,共9页
OBJECTIVE: To investigate whether Hydrolyzed Seawater Pearl tablet(HSPT) could modulate the Th1/Th2 imbalance in an immunosuppressed mouse model with Th1 to Th2 shift induced by Cyclosporine A(Cs A) which can be used ... OBJECTIVE: To investigate whether Hydrolyzed Seawater Pearl tablet(HSPT) could modulate the Th1/Th2 imbalance in an immunosuppressed mouse model with Th1 to Th2 shift induced by Cyclosporine A(Cs A) which can be used in the clinical treatment of Th2 to Th1 shift diseases, and explore the possible mechanism for the adjuvant therapeutic efficacy of HSPT on recurrent respiratory infections(RRI) and acquired immune deficiency syndrome(AIDS).METHODS: The mice were randomly divided into six groups of five animals each, namely normal group, model group, lentinan polysaccharide tablet(LPT) group and three HPST treated groups. HPST treated groups were administered with HPST(0.51,1.02, 2.04 g/kg) via intragastric gavage(i.g) for 30 consecutive days. LPT used as reference drug for positive control, LPT group was administered with LPT(8.2 mg/kg) for 30 consecutive days. Normal group and model group were received distilled water. The animals in model group, LPT group and HPST treated groups were injected intraperitoneally with Cs A(50 mg/kg) to establish the immunosuppressed mice model with Th1 to Th2 shift on the20 th, 22 nd and 24 th day, one hour after the administration of the respective treatment. Animals were sacrificed one hour after the last administration to collect blood and splenic tissue. The proportion of T cells including CD8+ and CD4+ T cells, Th1 and Th2 in peripheral blood of experimental mice were measured by flow cytometric. The protein level in serum and m RNA level in splenic tissue of experimental mice for interleukin(IL)-2, IL-12, interferon-γ(IFN-γ), IL-4, IL-6, IL-10 and IL-13 were measured by enzyme linked immunosorbent assay and fluorescence quantitative polymerase chain reaction respectively.RESULTS: HSPT elevated the proportion of T cells including both CD8+ and CD4+ T cells, in which the proportion of Th1 and Th2 cells increased, while the ratio of Th1/Th2 cells decreased in peripheral blood of the immunosuppressed mouse model with Th1 to Th2 shift induced by Cs A. Furthermore,HSPT elevated both protein and m RNA level of Th1-type cytokines IL-2 and IFN-γ, while had no significant effect on protein and m RNA level of Th1-type cytokine IL-12 and Th2-type cytokines IL-4, IL-6, IL-10, IL-13 in mouse model.CONCLUSION: Our findings suggest that HSPT can increase proportion of T cells including both CD8+and CD4+T cells and induce Th2 to Th1 shift in both cells and cytokines, which probably was the mechanism to account for the adjuvant therapeutic efficacy of HSPT on RRI and AIDS. 展开更多
关键词 respiratory tract infections acquired immunodeficiency syndrome th1-th2 balance INTERLEUKINS INTERFERON-GAMMA Hydrolyzed Seawater Pearl tablet
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Wang-Bi tablet, a patented Chinese medicine, maintains the balance of Th1/Th2 in mice with collagen-induced arthritis 被引量:5
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作者 Wang Jianming Luo Jing +4 位作者 Xu Yuan Yan Zeran Qu Xiangke Chen Mengxue Tao Qingwen 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2020年第3期401-406,共6页
OBJECTIVE: To investigate the pharmacological mechanism of Wang-Bi tablets(WBTs), a Chinese patented medicine, in rheumatoid arthritis(RA) using mice with collagen-induced arthritis(CIA).METHODS: A mouse model of CIA ... OBJECTIVE: To investigate the pharmacological mechanism of Wang-Bi tablets(WBTs), a Chinese patented medicine, in rheumatoid arthritis(RA) using mice with collagen-induced arthritis(CIA).METHODS: A mouse model of CIA was induced using bovine type Ⅱ collagen. WBT treatment was administered and efficacy was evaluated. The levels of interferon-γ(IFN-γ), interleukin-2(IL-2), and interleukin-4(IL-4) were examined using an enzyme-linked immunosorbent assay, and the proportions of Th1 and Th2 were detected using flow cytometry. T-bet and GATA-binding protein 3(GATA3)expression were demonstrated using Western blot analysis.RESULTS: Paw swelling and the arthritis index decreased significantly following WBT treatment. Histopathological analysis revealed markedly alleviated damage to synovium tissue in the WBT and methotrexate treatment groups. WBT regulated the production of IFN-γ, IL-2, and IL-4 and modulated Th1 and Th2 cell populations, which might have been induced by the attenuation of Th1 and Th2 cell differentiation through a decrease in the expression of T-bet and an increase in the expression of GATA3 in the synovial tissue in CIA mice.CONCLUSION: These results indicate that WBT may produce a therapeutic effect on CIA through maintaining the balance of Th1/Th2 cells, which could result in a decrease in the autoinflammatory disorder observed in RA. 展开更多
关键词 ARthRITIS RHEUMATOID Collagen th1-th2 balance Wang-Bi tablet
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Potential immunomodulatory role of sesamin in combating immune dysregulation associated with COVID-19
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作者 Amin F.Majdalawieh Sarah M.Yousef Imad A.Abu-Yousef 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第10期421-428,共8页
The ongoing outbreak of novel coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has caused an unprecedented global health crisis.Development of a cure for this de... The ongoing outbreak of novel coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has caused an unprecedented global health crisis.Development of a cure for this devastating disease is currently at full speed,with several vaccines against COVID-19 already authorized and administered.Currently,demand for these vaccines far exceeds supply.As such,boosting immunity represents a viable route to halt the rapid spread of SARS-CoV-2 and limit fatalities until vaccines become more readily and widely available.The use of phytochemicals appears to be a promising panacea.Sesamin,a lignan isolated from Sesamum indicum seeds,is known for its potent pharmacological properties,and is therefore hypothesized as a potential candidate in the therapeutic regimen against COVID-19.Herein,we highlight the confirmed therapeutic anti-inflammatory and immune-modulatory potential of sesamin against myriads of respiratory disorders,and tentatively suggest that sesamin may exert similar potent effects against COVID-19.Precisely,we speculate that sesamin may help alleviate COVID-19 via restoring Th1/Th2 balance and preventing inflammation and cytokine storm development.Additionally,we further support the promising role of sesamin against COVID-19 by underscoring the direct evidence,which suggests that sesamin may demonstrate promising inhibitory potential against three important SARS-CoV-2 targets,namely main protease,spike protein,and angiotensin-converting enzyme 2 receptor.Although preliminary,there is ample evidence to propose sesamin as a potential phytotherapeutic and prophylactic candidate against COVID-19.Further in vitro,in vivo,and preclinical studies are required to further substantiate the role of sesamin in the prevention and/or treatment of COVID-19. 展开更多
关键词 SESAMIN SARS-CoV-2 COVID-19 IMMUNOMODULATION ANTI-INFLAMMATORY Cytokine storm th1/th2 balance
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