Associations of PNPLA3 and LEP genetic polymorphisms with metabolic-associated fatty liver disease in Thai people living with human immunodeficiency virus

BACKGROUND The prevalence of metabolic-associated fatty liver disease(MAFLD)is a growing public health issue in people living with human immunodeficiency virus(PLWH).However,the pathophysiology of MAFLD is still unknown,and the role of genetic variables is only now becoming evident.AIM To evaluate the associations of gene-polymorphism-related MAFLD in PLWH.METHODS The study employed transient elastography with a controlled attenuation parameter≥248 dB/m to identify MAFLD in patients from a Super Tertiary Hospital in central Thailand.Candidate single-nucleotide polymorphisms(SNPs)were genotyped using TaqMan®MGB probe 5'nuclease assays for seven MAFLD-related genes.Statistical analyses included SNP frequency analysis,Fisher's Exact and Chi-square tests,odds ratio calculations,and multivariable logistic regression.RESULTS The G-allele carriers of PNPLA3(rs738409)exhibited a two-fold rise in MAFLD,increasing by 2.5 times in MAFLD with human immunodeficiency virus infection.The clinical features and genetic patterns imply that LEP rs7799039 A-allele carriers had a nine times(P=0.001)more significant chance of developing aberrant triglyceride among PLWH.CONCLUSION The current study shows an association between PNPLA3 rs738409 and LEP rs7799039 with MAFLD in PLWH.

Antioxidant Activities and Characterization of Polyphenols from Selected Northern Thai Rice Husks: Relation with Seed Attributes

Rice production generates a significant amount of agricultural waste. This study aimed to give results related to the existence of antioxidant phenols in agricultural waste of selected Northern Thai rice varieties. The antioxidant activities, contents of total flavonoids and phenolic compounds in the ethanolic rice husk extract were evaluated. The highest antioxidant activities were found in the variety PES1CMU, with 2,2’-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid and 2,2-diphenyl-1-picrylhydrazyl as 679.66 and 4.16 mmol/(L·g) trolox equivalent, respectively, ferric reducing antioxidant power as 0.87 mmol/(L·g) Fe2+, total phenolic content as 29.90 mmol/(L·g) gallic acid and total flavonoid content as 12.16 mg/g catechin equivalent. Polyphenol compounds were identified mainly by standard polyphenols using the liquid chromatography mass spectrometry, with the highest contents of phytic acid, o-coumaric acid, naringin and kaempferol. The non-glutenous and wetland ecotypes of rice husk samples were the richest in antioxidant activities and polyphenol contents characterized by using principal component analysis. The glutenous rice husk contained higher antioxidant activities than the rest. Interestingly, quercetin is a significant phenolic compound that positively correlated with the overall antioxidant activities of rice husk. This finding will be relevant for future application of rice husk antioxidant components in the production of functional ingredients as well as for the food and pharmaceutical industries.

The History and Development of Muaythai Boran

The purpose of this research was to develop the treatise of traditional Muay Thai （traditional Thai boxing）. This is a document research conducted from documents, books, researches, journals and various data from websites. It analyzed traditional Muay Thai into 6 issues, including history and development, benefits and values, methodologies and traditions, Muay Thai practicing, traditional Mae Mai Muay Thai, as well as the identity of Muay Thai base on sport science. The results of this research indicated that the history and the development of Muay Thai have been starting since the Sukhothai period. Also, the practicing of Muay Thai is very significant for close range battle. During the Sukhothai period, almost of boys who are going to be teenagers like to practice Muay Thai as a weapon to protect themselves. The practicing of Muay Thai is also a part of fighting-skilled people which included qualification, courtesy, humanity, bravery, patience, power of black magic, energy, martial art in Muay Thai, and Krabi Krabong; traditional Thai martial art in combination with weapons. Moreover, Ayutthaya Kingdom had to make major war for 39 times related to the military conflicts and the resistance of an invasion from neighboring kingdoms such as Sukhothai, Burma, Khmer and Mon. Most of all was the Burmese-Siamese War. Consequently, the king and Thais had to practice the martial arts and to learn the strategies for defending the land. According to section one of the 112 law, Thais used their parts of body as a weapon to battle with enemies. In fact, this martial art has been playing since Somdet Phra Ekatotsarot reign. Furthermore, Somdet Phra Chao Taksin Maha Rat （King of Thonburi） at that time is really interested in Muay Thai. Also, he has good skills in Muay Thai, and Krabi Krabong. When he was 6, Somdet Phra Chao Taksin Maha Rat attended to study at War Kosawat （Wat Klang） and practiced his Muay Thai skill from the military in the grand palace During Rattanakosin Period （1788）, according to historical record, there were two French steersmen who have well-skilled in Muay that came into Thailand to battle Muay skill with Thais. At that time, there was Muen Phalan; who is a military in the grand palace that has well-skilled in Muay Thai, was selected to fight with the two French steersmen. Additionally, at this period, there was found major evidence which is really significant related to Muay Thai--the treatise of traditional Muay Thai.

THAI技术开发厚层稠油油藏井网参数优选

厚层稠油油藏原油黏度高、埋藏深、油藏流体流动性差、动用程度低,采用THAI(从端部到跟部注空气)技术可提高该类油藏的采收率。通过数值模拟对排状布井方式的井网参数进行优选,得出影响因素的敏感性强弱依次为生产井水平段长度、注气量、井距、注气井到生产井水平段端部的距离。在此基础上,进一步分析厚层稠油油藏中不同布井方式的开发效果。结果表明:随着燃烧前缘向前推进,生产井中会产生气窜通道;方案2VIHP的采油速度和采收率优于其他方案;层厚是影响热量传递的重要因素;垂直注气井的开采效果优于水平注气井。

THAI技术及其在稠油开发中的应用

针对常规火烧油层工艺中使用垂直注入井与生产井进行长距离驱替的缺点,开发了一种新的短距离驱油技术:从水平井端部到跟部注空气技术(THAI)。应用THAI技术在驱油过程中可实现就地升温降粘和非混相空气驱,尤其适合于稠油和超稠油的开采。介绍了THAI技术的原理、与其他热采方式相比的主要优势;通过对THAI技术影响因素的分析,给出了实现该技术的关键工艺。

Detection of Purity of Thai Hom Mali Rice by RAPD

[Objective] The aim was to establish more specific, sensitive, accurate and practical method to detect purity of Thai Hom Mali rice. [Method] RAPD method cooperated with two primers of KDML105 and RD15 was established by feeling for the extraction methods of DNA, optimizing concentrations of the factors influencing the results of RAPD such as template DNA, Mg2＋, random primer, dNTPs and Taq polymerase, and screening the random primers. [Result] The optimum RAPD reaction system was 25.0 μl in total volume, containing 4.0-32.0 ng/μl of template DNA, 200.0 μg/L random primer, 2.0 mmol/L Mg2＋, 200.0 μmol/L dNTPs and 1.0 U of Taq enzyme. Then, the Thai Hom Mali rice and non-Thai Hom Mali rice can be distinguished according to the presence or absence of two DNA markers. [Conclusion] The RAPD technology can effectively cover the shortages of identifications by sense and boiling in water; in addition, it is simple, sensitive and low-cost, suitable to be used in routine tests.

Trends in the prevalence of obesity among young Thai men and associated factors:From 2009 to 2016

Background:The prevalence of obesity has been increasing in both males and females worldwide.In Thailand,the National Health Examination Surveys reported that the prevalence of obesity(body mass index(BMI)≥30 kg/m^2)among Thai male adults aged 20–59 years increased from 1.7%in 1991 to 6.8%in 2009.Obesity has been confirmed to lead to health problems,including noncommunicable diseases.In the present study,we report trends in the prevalence of obesity among new conscripts from 2009 to 2016.We also investigated the associated factors of obesity.Methods:Serial cross-sectional studies were conducted from 2009 to 2016 among male Royal Thai Army(RTA)conscripts whose weight and height had been measured to determine BMI after being inducted.Each subject completed a detailed risk factor questionnaire.Obesity was defined as BMI≥30 kg/m2.Results:A total of 26,540 young Thai males conscripted into the RTA were included in this study.The prevalence of obesity was 2.2%in 2009,3.4%in 2010,2.5%in 2011,2.9%in 2012,3.4%in 2013,4.4%in 2014,5.0%in 2015,and 4.8%in 2016(P for trend<0.0001).The independent risk factors for obesity were coming from the north central and south regions compared with the northeast,higher education level,indoor occupation and no regular exercise.Conclusions:Our data emphasized that obesity constitutes a serious problem among young Thai men.We could apply these findings in military units to other groups at any age.Regular exercise should be provided to young adults and other age groups to slow the process of obesity,so that associated complications,especially noncommunicable diseases,will cease.

＂Why Do They Drink？＂ Alcohol Uses and Opinions Towards Alcohol Marketing Communication Messages of Thai Teens

Problem about adolescent drinking has accelerated. Although the alcohol advertisements are restricted by Thai law, nevertheless the alcohol manufactures could still find their way to convey the message about their products to their consumers, including the adolescents in the school system through several marketing communication tactics. To this study, investigated opinion of Thai teens on alcohol use specific to.. （1） types of alcoholic beverages preferred; （2） reason at first alcohol use; （3） future drinking intention; and （4） perceptions toward alcohol marketing communication. The cross-sectional survey was conducted on 5184 students from grades 4th-12th and vocational school in seven regions of Thailand. The data from questionnaires were analyzed using simple descriptive statistic, frequency distribution, and percentages. Those teens reported to start drinking beer because of wanting to experiment with alcohol （41.3%）, to celebrate in special occasion （29.2%）, and due to friends involved （15.7%）. Majority of teens in this study （〉 80%） disagree or strongly disagree with persuasive message of alcohol advertised through several media channels. Most of the samples have good media literacy, know the intention of the marketing communication of the alcohol manufacturers, and could not be persuaded easily. The results required further evaluation for influence of alcohol marketing communication on students＇ alcohol expectancy and alcohol use for proper policy recommendation and management.

Tourist Industry Promotion： Aim to Introduce Thai Local Foods Matching With Northern Program＇s Tours to Serve Sustainable Tourism

Tourist industry had been promoted and become one of the important strategies of Thai government for socioeconomic development for years and become successful by ranking--Thailand becomes well-known as top ranking to visit from tourists around the world. When people travel, foods are always needed to fulfill human needs and the satisfaction of the travelers. The objectives of the research were to： （1） survey and determine the local food image and identity of Northern region of Thailand to support tourist industry; （2） survey program＇s tourism in Thailand and study the promotion of local food that had added in the programs＇ tours as tourist destination; （3） find out the possibility of local food and culture for promotion in tourist industry of Northern region in Thailand; （4） develop gastronomy tourism＇s routing. The population were the subject persons who are experts in tourist industry, Northern foods restaurant owner, and program＇s tour. Mixed method was used for data collection and analysis. The result of the research is revealed into four parts： Phase 1： （1） Thai Northern region had been divided into two part： upper Northern and lower Northern region. There are eight provinces in upper Northern region： Chingmai, Chingrai, Lampoon, Lampang, Prayao, Phare, Nan, and Mae-Hongsorn. Chingmai was the capital of ＂Lanna territory （1558-1774）＂ rich with culture; （2） Upper Northern local foods related to the legend, have their own characters and are still available to get to eat; （3） Northern local food contained with Northern ingredient and indicated as geographical indications; （4） The lower Northern region related with ＂Sukhothai territory （1157-1370）＂, Sukhothai was the capital. There are six provinces in the region： Sukhothai, U-taradid, Tag, Kampangpet, Pidsanuloak, and Petchaboon. Food of the lower Northern provinces had their own characteristics but some of the dishes were influenced with Chinese cuisine, such as noodles. Phase 2： Most of tour companies had included local food in the tour programs to promote Northern local foods in low level. Phase 3： Life＇s pathway of the Northern people lived related to the three seasons： rainy season, cool season, and summer. Each season had different kinds of vegetables and fruits which influence the menus and foods. Eating foods as medicine also related to the season, such as shallot and garlic had added more in foods in cool season, in order to prevent cold of family＇s members. Northern life style always celebrated in all ceremonies with foods, snack, and sweet, such as： wedding, cremation, and community cerebrations which may be promoted in tour program as their ways of living with sustainable, in order to promote socioeconomic development in the community. Phase 4： The gastronomy tourism was developed into five routings to promote tourist industry in the Northern part of Thailand as the following： （1） gateway to reach ＂Lanna＂; （2）window to visit the Northern part of Thailand; （3） pay respect to the Buddha and visit dynasty heritage; （4） visit the peaceful and tranquilly heritage; and （5） visit Thailand＇s border with boundless. Conclusion： Thai local lifestyle, culture, and local foods in the Northern part of Thailand, contained rich image and identity of history, legend, and human diversity, which are worth and valuable for promoting as tourist industry in the Northern part of Thailand and also conserved job absorption and community development. Therefore the government of Thailand and tour companies ought to promote Thai local foods as value added and tourist industry which also benefit for the sustainable socioeconomic development in long future.

HOME AWAY FROM HOME--The growth and passion of a Thai student in China

Born and raised in Thailand, I had never imagined forging a special bond with China. In my mind, China was a distant and mysterious place with strong appeal, and I always thought I would see as many places as possible if I ever found the chance to visit the country.

厦门海域岩相海岸带疣荔枝螺(Thais clavigera)与甲虫螺(Cantharus cecillei)性畸变

海产腹足类性畸变以其对有机锡的敏感性、特异性和不可逆性而成为海洋有机锡污染的理想指示种。调查研究了厦门海域岩相海岸带疣荔枝螺(Thais clavigera)和甲虫螺(Cantharus cecillei)的性畸变情况,通过生物学指标IOI(性畸变率)、RPSI(相对阴茎发展指数)、VDSI(输精管发展指数)及SRI(性比指数)综合评价其性畸变发展程度,间接指示厦门周边海域的有机锡污染程度。研究结果发现,在采集疣荔枝螺的17个站点中有5个站点种群性畸变率为100%,其中宝珠屿种群性畸变程度最为严重,VDSI达4,采集甲虫螺的4个站点的种群性畸变率均为100%。性畸变程度较严重的种群普遍集中于厦门西港,并且表现出由港内到港外逐渐减轻的趋势,而白城、会展中心和大嶝岛等位于开阔的厦门东海域的种群性畸变程度最轻,与往年厦门西海域有机锡污染监测数据相比一致,体现出性畸变程度与有机锡污染程度及海港码头远近之间的相互关系。

疣荔枝螺(Thais clavigera)性畸变现象的解剖学和组织学研究

以解剖学和组织学的方法研究了有机锡污染生物指示种———疣荔枝螺的正常雄、雌和性畸变个体的生殖系统。结果表明 ,性畸变个体除具有正常的雌性器官外 ,还有输精管或阴茎。当性畸变程度严重时 ,性畸变不育个体的卵囊腺组织出现破损 ,并具有不规则块状的不育卵囊和未排出的正常卵囊。虽然性畸变个体的雄性器官具有表现出雄性功能的潜能 ,但由于缺少精巢或完整的前列腺 ,不可能发展成为具有雄性功能的个体 ,这与性逆转有着本质上的区别。有机锡污染引起的性畸变已对疣荔枝螺种群生存构成了潜在的威胁 ,深化此类负面生态效应的研究是人类面临的重要课题。

食物相暴露下三丁基锡(TBT)在疣荔枝螺(Thais clavigera)体内的吸收和代谢动力学

将牡蛎消化腺分别暴露在1000ng.L-1和100ng.L-1TBT水溶液中4周,然后将染毒的牡蛎消化腺分别投喂疣荔枝螺(Thais clavigera)。经过45d的暴露和30d的净化,我们发现雌雄疣荔枝螺的消化和生殖系统能较快地吸收TBT(吸收速率ku=0.004-0.022.d-1),并且其代谢(生物代谢系数BDI=5.59-23.30)和排出速率(净化速率ke=0.024-0.053.d-1)也相对较快,各器官中TBT的代谢产物MBT占了相对较高的比例,因此TBT在食物链传递过程中没有出现生物放大的现象。此外,TBT有逐渐从雌螺消化系统向生殖系统转移的趋势,并且雌螺生殖系统对TBT的吸收和富集能力(ku=0.006-0.022.d-1,生物放大系数BMF=0.181-0.664)要显著强于雄螺(ku=0.004-0.014.d-1,生物放大系数BMF=0.142-0.376),但其代谢和净化速率(BDI=5.59-10.50,ke=0.024-0.025.d-1)却显著低于雄螺(BDI=11.5-12.4,ke=0.031-0.050.d-1),雌螺的生殖系统被认为是TBT转移和富集的潜在靶器官,这对我们今后开展TBT污染的环境监测和评价具有重要的参考价值。

Rate of anticoagulant use, and factors associated with not prescribing anticoagulant in older Thai adults with non-valvular atrial fibrillation: A multicenter registry

Objectives To investigate the rate of anticoagulant use,the reasons for not prescribing anticoagulant,and the factors associated with non-prescription of anticoagulant in older Thai adults with non-valvular atrial fibrillation.Methods A multicenter registry of patients with non-valvular atrial fibrillation was conducted during 2014 to 2017 in Thailand.Demographic,medical history,antithrombotic medication,non-antithrombotic medication,and laboratory data were collected and analyzed.Data were compared between the older adult (≥ 65 years) and younger adult (< 65 years) groups.The reasons why anticoagulant was not prescribed were collected,and predictive factors were identified.Results A total of 3218 patients (1873 males) with an average age of 67.3 ± 11.3 years were included.Almost two-thirds (61.0%) of patients were in the older adult group.Anticoagulant was prescribed in 2422 patients (75.3%): 81.4% in the older adult group and 65.7% in the younger adult group.The three main reasons for not prescribing anticoagulant were already taking antiplatelets,patient refusal,and bleeding risk.These reasons were more common in older adults as compared to younger adults.Multivariate analysis revealed current use of antiplatelets to be the most important factor that predict the non-prescription of anticoagulant in older population.Conclusions The prevalence of anticoagulant prescription among older Thai adults with atrial fibrillation is 81.4%.Taking antiplatelet drugs was found to be the strongest reason that predicts the non-prescription of anticoagulant in this patient population.A guideline should be developed to optimize the use of anticoagulant and antiplatelet in older adults.

疣荔枝螺(Thais clavigera Kuster)人工繁育技术研究

本实验开展了疣荔枝螺(Thais clavigera Kuster)室内全人工繁育技术研究;具体包括亲螺人工促熟蓄养、卵囊采集、孵化、幼虫培育、变态和采苗等技术研究,同时,还开展了后期面盘幼虫对不同附着基的喜好选择性实验,以及不同饵料和光照对幼虫附着的影响研究。研究表明:雌螺分批产卵,单个雌螺平均每次产出受精卵为61 750粒;在水温27~29℃时,面盘幼虫经过20d左右的生长发育,壳长达到约600μm,此时开始附着变态,由浮游生活转变为底栖生活;附着后10 d左右变态为稚螺。后期面盘幼虫对附着基的选择性实验表明,固着牡蛎苗的栉孔扇贝壳和附有底栖硅藻的波纹板,是稚螺理想的附着基。不同饵料和光照对幼虫附着影响实验结果表明:在附着变态期间,自然光照有利于幼虫附着变态;同时,连续投喂糠虾肉糜可显著提高幼虫变态率和稚螺成活率。本研究结果为今后疣荔枝螺产业化繁育提供了重要的理论和技术支持。

A Thai family with hereditary pancreatitis and increased cancer risk due to a mutation in PRSS1 gene

AIM: To investigate mutation of serine protease 1-cationic trypsinogen (CT, PRSS1) gene in members of a Thai family with hereditary pancreatitis and pancreatic cancer. METHODS: Polymerase chain reaction and direct sequencing were performed to analyze the PRSS1 gene in two members of the family affected by pancreatitis. Allele specific amplification (ASA) method was then developed to detect the mutation of the PRSS1 gene in all available members of the family and normal control subjects. RESULTS: A cytosine (C) to thymine (T) mutation at position 2441 (g.2441C>T) of the PRSS1 gene, which results in a substitution of arginine by cysteine at position 116 (R116C) of CT, was identified by direct sequencing in both clinically affected members of the family but was not found in the unaffected member. This mutation, which might be arising from deamination of methylated cytosine in CpG dinucleotide of codon 116 (CGT>TGT), was also detected by the ASA method in the two affected members and a proband's brother but was not observed in unaffected members and 54 normal control subjects. CONCLUSION: Autosomal dominant pancreatitis with increased cancer risk in the studied Thai family is most likely due to missense (R116C) mutation in the PRSS1 gene.

Crustal strain rates of southeastern Tibetan Plateau derived from GPS measurements and implications to lithospheric deformation of the Shan-Thai terrane

The link between the crustal deformation and mantle kinematics in the Tibetan Plateau has been well known thanks to dense GPS measurements and the relatively detailed anisotropy structure of the lithospheric mantle.However, whether the crust deforms coherently with the upper mantle in the Shan-Thai terrane(also known as the Shan-Thai block) remains unclear.In this study, we investigate the deformation patterns through strain rate tensors in the southeastern Tibetan Plateau derived from the latest GPS measurements and find that in the Shan-Thai terrane the upper crust may be coupled with the lower crust and the upper mantle.The GPS-derived strain rate tensors are in agreement with the slipping patterns and rates of major strike-slip faults in the region.The most prominent shear zone, whose shear strain rates are larger than 100×10^(–9) a^(–1), is about 1000-km-long in the west, trending northward along Sagaing fault to the Eastern Himalayan Syntaxis in the north, with maximum rate of compressive strain up to –240×10^(–9) a^(–1).A secondary shear zone along the Anninghe-Xiaojiang Fault in the east shows segmented shear zones near several conjunctions.While the strain rate along RRF is relatively low due to the low slip rate and low seismicity there, in Lijiang and Tengchong several local shear zones are present under an extensional dominated stress regime that is related to normal faulting earthquakes and volcanism, respectively.Furthermore, by comparing GPS-derived strain rate tensors with earthquake focal mechanisms, we find that 75.8%(100 out of 132) of the earthquake T-axes are consistent with the GPS-derived strain rates.Moreover, we find that the Fast Velocity Direction(FVDs) at three depths beneath the Shan-Thai terrane are consistent with extensional strain rate with gradually increasing angular differences, which are likely resulting from the basal shear forces induced by asthenospheric flow associated with the oblique subduction of the India plate beneath the Shan-Thai terrane.Therefore, in this region the upper crust deformation may be coherent with that of the lower crust and the lithospheric mantle.

Characterization of the salivary microbiome in healthy Thai children

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated saliva samples. Subsequently, amplicon libraries were prepared by 16 S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses.Results: The correlation between oral hygiene status and caries history varied from r^2=0.887 to r^2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups(P<0.05). Furthermore, the proportion of Veillonella parvula, as well as Rothia aeria and Rothia dentocariosa tended to increase with worse oral hygiene status, which was also related to higher dental caries history.Conclusions: The differences in the salivary microbiome as related to geographic regions suggest that environmental factors, which may include dietary habits, could influence the predominant bacteria found in the mouth of Thai children, especially the genus Veillonella and Prevotella. The ratio of Veillonella parvula, Rothia aeria and Rothia dentocariosa may be indicators of worse oral hygiene status and future caries in this population.

Development and characterization of seed gums from Tamarindus indica and Cassia fistula as disintegrating agent for fast disintegrating Thai cordial tablet

Crude seed gum and their carboxymethyl derivatives from Tamarindus indica and Cassia fistula seeds were developed and characterized to apply as the pharmaceutical disintegrant in fast disintegrating Thai cordial tablet. The chemical structure of crude gum was chemically modified via carboxymethylation. Degree of substitution(DS) of carboxymethylated gums was determined. Carboxymethylated gums with minimum and maximum DS values were chosen for further application. IR absorption spectra of gum samples were observed to verify their chemical structure changes. In physical properties, the intrinsic viscosity and swelling property of all gum samples were evaluated. The results showed that carboxymethylated gums had higher intrinsic viscosity than those of crude gum. Moreover, they could swell and be soluble in cold water better than those of crude gums. In conclusion, the modified gums from both plants could provide higher hardness and be better used than that crude gums for fast disintegrating Thai cordial tablet. However, this is a preliminary assessment to expressing pharmaceutical application possibility of these gums as disintegrants, diluents and drug release controlling agents.

New Thai herbal shampoos as pediculicides for killing head louse, Pediculus humanus capitis De Geer(Phthiraptera)

Objective: To evaluate the efficacy of Thai herbal shampoos [Cartoxylum formosum(C.formosum) + eucalyptus essential oil(EO), C. formosum + citrus EO, Solanum trilobatum+ eucalyptus EO, Solanum trilobatum + citrus EO, Moringa oleifera + eucalyptus EO and Moringa oleifera + citrus EO] for killing all stages of Pediculus humanus capitis(Phthiraptera).Methods: A filter paper contact method was applied with three concentrations(0.05, 0.10 and 0.20 mL/cm^2) of each Thai herbal shampoo as well as permethrin pediculicide(positive control) and drinking water(negative control) against eggs, nymphs and adults of Pediculus humanus capitis. Mortality rates of the eggs were recorded after 7 days of incubation while those of nymphs and adults were recorded after 5 minutes of contact. Results: All herbal shampoos at the high concentration were highly effective against nymphs and adults, but not effective against the eggs. C. formosum + eucalyptus EO and C. formosum + citrus EO shampoos at all concentrations exhibited the highest efficacy against nymphs and adults with100% mortality rate at 5 min and LC_(50) values of 0.004 and 0.005 mL/cm^2, respectively. All formulation of Solanum trilobatum and Moringa oleifera shampoos added with eucalyptus EO showed mortality rates against nymphs at 92.0%-100.0% and 76.0%-100.0% and against adults at 84.0%-100.0% and 20.0%-32.0%, respectively. Permethrin pediculicide was not effective against the eggs, but showed 68.0%-92.0% and 28.0%-60.0% mortality rates against nymphs and adults. Conclusions: These results indicate that C. formosum + eucalyptus EO shampoo can be used as an effective nymphicide and adulticide against Pediculus humanus capitis.