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Investigation of Optimal Charcoal Concentration for Heterokaryon Formation of Rhizoctonia solani
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作者 P. Qu M. Hyakumachi 《Journal of Agricultural Science and Technology》 2010年第2期8-13,共6页
Optimal charcoal concentrations were examined for tuff formation of SBIs (single-basidiospore isolates) obtained from Rhizoctonia solani AG-1 IC, AG-2-2 IV and AG-2-3, and SPIs (single-protoplast isolates) from AG... Optimal charcoal concentrations were examined for tuff formation of SBIs (single-basidiospore isolates) obtained from Rhizoctonia solani AG-1 IC, AG-2-2 IV and AG-2-3, and SPIs (single-protoplast isolates) from AG-1 IA by using PDCA with different charcoal concentrations. The best charcoal concentration for SBIs from AG-1 IC was 0.5%, from AG-2-3 was 1%, and from AG-2-2 IV was 2%. The optimal concentration was 0% for SPIs from AG-1 IA. The optimal charcoal concentrations used for tuff formation between SBIs and between SPIs are quite varied, depending on the different AGs. The results of AFLP haplotypes suggest that tuff isolates formed between SBIs/SPIs are heterokaryon. 展开更多
关键词 Heterokaryon formation charcoal concentration thanatephorus cucumeris
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