Effect of combined therapy of Yinchenhao Chengqi decoction and endoscopic sphincterotomy for endotoxemia in acute cholangitis

AIM To evaluate the therapeutic mechanism of Yinchenhao Chengqi (YCHCQ) decoction (containing mainly Herba Artemisia capillaris) combined with endoscopic sphincterotomy (EST) for endotoxemia (ETM) in acute cholangitis.METHODS Twenty-one cases of acute cholangitis with ETM were divided randomly into two groups: group A, 10 patients treated with YCHCQ decoction combined with EST, group B, 11 patients treated with EST. The incidence rate of ETM, plasmic ET, serum superoxide dismutase (SOD) activity, malonyldialdehyde (MDA), complement C3 and C-reactive protein (CRP) were studied respectively.RESULTS The ET level of group A (35.92ng/L±8.30ng/L) was significantly reduced after 7 days of treatment (P＜0.05) in contrast to that of group B (47.8ng/L±11.62ng/L), so did the level of MDA and CRP. But the SOD activity and C3 level in group A increased significantly (P＜0.05).CONCLUSION YCHCQ decoction combined with EST had a beneficial effect for ETM in acute cholangitis.INTRODUCTIONEndotoxemia (ETM) is one of the most important physiopathologic causes of acute cholangitis and it is the trigger of cytokines and inflammatory factors. In recent studies it has been found that Yinchenhao Chengqi (YCHCQ) decoction has a beneficial effect on ETM in acute cholangitis. With the development of endoscopic surgery, endoscopic sphincterotomy (EST) has become an effective replacement for some operations in the treatment of acute cholangitis[1]. The effect of YCHCQ decoction combined with EST on ET, oxygen free radical and complement C3 was observed in order to find out its therapeutic mechanism.

Apoptosis of human pancreatic carcinoma cell-1 cells induced by Yin Chen Hao Decoction

AIM: To evaluate human pancreatic carcinoma cell line(PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction(YCHD).METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosisassociated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse transcription-PCR.RESULTS: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96 h, expression of BAX m RNA increased gradually and BCL-2 m RNA reduced gradually with time.CONCLUSION: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.

Effect of Danggui Buxue Decoction on the expression of VDR,CYP27B1,CYP19,and ERβin the ovary of osteoporosis rats

Investigate the effects of the classic ancient prescription Danggui Buxue decoction on the vitamin D receptor,25-hydroxyvitamin D-1αhydroxylase,estrogen synthesis aromatase and estrogen receptorβmRNA and protein expression in osteoporotic rats.The classic ancient prescription Danggui Buxue decoction may affect the levels of estrogen synthesis aromatase,estrogen receptorβ,FSH,and Klotho in the body through regulating the expression of ovarian vitamin D receptor and 25-hydroxyvitamin D-1αhydroxylase to achieve the therapeutic effect of retinoic acid-induced osteoporosis in rats.The results of this experiment showed that compared with the control group,the serum FSH level of the model group was significantly increased,the Klotho level was decreased,the relative expression of ovarian tissue CYP27B1,CYP19,ERβmRNA and protein was significantly reduced,and the relative expression of vitamin D receptor mRNA and protein was significantly rise.After the intervention of the classic ancient prescription Danggui Buxue decoction and vitamin D,compared with the model group,the serum FSH content decreased,Klotho content increased,ovarian CYP27B1,CYP19,ERβmRNA and protein expression significantly increased,ovarian vitamin D receptor mRNA and protein expression significantly decreased.Based on the effect of the classic ancient prescription Danggui Buxue decoction on the vitamin D system,combined with the results of this experiment,it is concluded that the classic ancient prescription Danggui Buxue decoction may affect the levels of CYP19,ERβ,follicle-stimulating hormone,and Klotho in the body by regulating the expression of ovarian VDR and CYP27B1.The specific mechanism of the treatment of ovarian injury in osteoporosis rats needs further study.Background:Investigate the effects of the classic ancient prescription Danggui Buxue decoction on the vitamin D receptor,25-hydroxyvitamin D-1αhydroxylase,estrogen synthesis aromatase and estrogen receptorβmRNA and protein expression in osteoporotic rats.Methods:The experiment was divided into 6 groups:control group,model group(retinoic acid 100 mg/kg/d),vitamin D group(calcitriol 30 ng/kg/d),the classic ancient prescription Danggui Buxue decoction high,middle and low dose group(7.2 g/kg/d,3.6 g/kg/d,1.8 g/kg/d).Enzyme-linked immunosorbent assay method was used to detect the effects of different doses of the classic ancient prescription Danggui Buxue decoction on serum follicle-stimulating hormone and Klotho levels in retinoic acid-induced osteoporosis rats;real-time fluorescence quantitative PCR was used to detect the classic ancient prescription Danggui Buxue decoction on retinoic acid effect of ovary vitamin D receptor,25-hydroxyvitamin D-1αhydroxylase,estrogen synthesis aromatase,estrogen receptorβmRNA expression in induced osteoporosis rats;Western-blot detection of the classic ancient prescription Danggui Buxue decoction on retinoic acid-induced osteoporosis ovary vitamin D receptor,CYP27B,estrogen synthesis aromatase,estrogen receptorβprotein The impact of expression.Results:Compared with the control group,the serum follicle-stimulating hormone level of the model group was significantly increased,the Klotho level was decreased,the relative expression levels of 25-hydroxyvitamin D-1αhydroxylase,estrogen synthesis aromatase,estrogen receptorβmRNA and protein in the ovarian tissue were significantly decreased,and the relative expression levels of vitamin D receptor mRNA and protein were significantly increased.After the intervention of the classic ancient prescription Danggui Buxue decoction and vitamin D,compared with the model group,the serum follicle-stimulating hormone content decreased,Klotho content increased,ovarian 25-hydroxyvitamin D-1αhydroxylase,estrogen synthesis aromatase,estrogen receptorβmRNA and protein expression significantly increased,ovarian vitamin D receptor mRNA and protein expression significantly decreased.Conclusion:The classic ancient prescription Danggui Buxue decoction may affect the levels of estrogen synthesis aromatase,estrogen receptorβ,follicle-stimulating hormone,and Klotho in the body through regulating the expression of ovarian vitamin D receptor and 25-hydroxyvitamin D-1αhydroxylase to achieve the therapeutic effect of retinoic acid-induced osteoporosis in rats.

Effect of Modified and accumulated decoction on serum ER, PR, sex hormone, IGF-I and IGF-I in patients with uterine fibroids

Objective: To study the effect of Modified and accumulated Decoction on serum ER, PR, sex hormone, IGF-I and IGF-I in patients with uterine fibroids. Method: A total of 90 patients with uterine fibroids in our hospital from January 2015 to January 2017 were enrolled in this study. The subjects were divided into the control group (n=45) and the treatment group (n=45) randomly. The control group was treated with mifepristone, the treatment group was treated with Modified and accumulated Decoction combined with mifepristone, and both the two groups were treated for 3 months. The uterine fibroid volume and uterine volume of the two groups before and after treatment were compared. The serum ER, PR, P, E2, LH, FSH, IGF-I and IGF-IR of the two groups before and after treatment were compared. Result: There were no significantly differences of the uterine fibroid volume and uterine volume of the two groups before treatment. The uterine fibroid volume and uterine volume of the two groups after treatment were significantly less than before treatment, and that of the treatment group after treatment were significantly less than the control group. There were no significantly differences among the serum ER, PR, P, E2, LH, FSH, IGF-I and IGF-IR of the two groups before treatment. The serum ER, PR, P, E2, LH, FSH, IGF-I and IGF-IR of the two groups after treatment were significantly lower than before treatment, and that of the treatment group after treatment were significantly lower than the control group. Conclusion: Modified and accumulated Decoction combined with mifepristonecan significantly reduce the uterine fibroid volume, improve the serum ER, PR, sex hormone, IGF-I and IGF-I levels of the patients with uterine fibroids, and it was worthy clinical application.

香砂六君子汤对脾虚高脂血症大鼠内质网应激PERK信号通路的影响

目的探讨香砂六君子汤对脾虚高脂血症大鼠内质网应激蛋白激酶R样内质网激酶(protein kinase R-like ER kinase,PERK)信号通路的影响。方法SD大鼠随机分为4组,即正常对照组、脾虚高脂组、香砂六君子汤组和辛伐他汀组,后3组采用饮食不节加力竭游泳的复合方法造模15 d后,正常组喂饲基础饲料,其余3组喂饲高脂饲料。12周后,分别给予相对剂量药物和生理盐水。4周后,检测各组大鼠血脂含量、D-木糖排泄率,油红O染色观察肝脏脂质沉积情况,实时荧光定量PCR及Western blot法检测大鼠肝脏葡萄糖调节蛋白78(glucose regulated protein 78 kD,GRP78)、PERK和活化转录因子4(the activating transcription factor 6,ATF4)基因及蛋白表达。结果与正常对照组比较,脾虚高脂组大鼠血清脂质异常,表现为总胆固醇(cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇酯(low density lipoprotein-cholesterol,LDL-C)明显升高,高密度脂蛋白胆固醇酯(high density lipoprotein-cholesterol,HDL-C)明显降低,尿D-木糖排泄率明显降低;肝脏脂质沉积显著,可见明显脂滴;肝脏GRP78、PERK和ATF4 mRNA水平及蛋白表达水平显著升高。与脾虚高脂组比较,香砂六君子汤组和辛伐他汀组大鼠血清TC、TG、LDL-C明显降低,HDL-C明显升高,尿D-木糖排泄率明显升高;其肝脏脂质沉积情况有明显改善,脂滴不同程度减轻;肝脏GRP78、PERK和ATF4 mRNA水平及蛋白表达水平明显降低。结论香砂六君子汤可能通过调控内网应激PERK信号通路来纠正脾虚脂质紊乱的状态。

Poetic Comnos er Chen Qigang

ON visiting Chen Qigang’s web page(www.qigangchen.fr.st) and seeing his picture,the viewer knows immediately that this is noordinary person. Chen’s essential boldness,sensitivity and warmth show clearly in his face. Onreading his resume about his studies in France, and hiscompositions that were a departure from traditionalChinese music, this impression is endorsed. His tutor,Olivier Messiaen, a master of 20th century modernmusic, once remarked, "His compositions reveal truecreativeness, great talent, and a perfect assimilation ofEuropean musical concepts into Chinese thought."On his emergence into contemporary classical music, few of Chen’s peers acknowledged the originality of his work. Years later, his talent, unconventional-ity, and refusal to be constrained by contemporary Western musical mores have won him recognition. In January 2003, the UK record company EMI/Virgin is to release Chen Qigang’s CD (The Five Elements, Reflections on Years Past, and Butterfly in Love with Flowers).

陈氏清肝解毒汤治疗肝经郁热型带状疱疹临床观察

目的:观察陈氏清肝解毒汤治疗肝经郁热型带状疱疹的临床效果。方法:选择上海市中医医院治未病科2020年1月~2022年12月带状疱疹患者75例进行研究,随机将其分为两组,其中对照组(n=37)接受盐酸伐昔洛韦片+甲钴胺片+炉甘石洗剂治疗,治疗组(n=38)加用陈氏清肝解毒汤,均实施为期10d治疗。观察两组10d治疗结束后的临床治疗效果;对比治疗前后的症状量化评分;对比治疗前,治疗后3d, 7d, 10d疼痛情况(VAS)评分;对比水疱停止增加时间、病灶结痂时间和脱痂时间;对比治疗前后血清水痘-带状疱疹病毒IgM抗体(VZV-IgM);对比不良反应情况;对比治疗后3个月带状疱疹后遗神经痛(PHN)的发生率。结果:治疗组10d治疗结束后临床疗效总有效率为90.32%,且高于对照组80.00%(P<0.05);10d药物治疗完成后,治疗组症状量化评分低于对照组(P<0.05);治疗后7d, 10d,治疗组疼痛低于优于对照组(P<0.05);与对照组相比,治疗组患者经药物治疗后水疱未增多、病灶结痂及脱痂时间均较低(P<0.05)。10d药物治疗完成后,治疗组患者VZV-IgM低于对照组(P<0.05);两组不良反应发生率相同(P>0.05)。治疗组治疗后3个月PHN发生率低于对照组(P<0.05)。结论:陈氏清肝解毒汤加入西药治疗后,不仅能有效改善肝经郁热型带状疱疹患者临床疗效,同时对病程缩短、疼痛减轻及减少PHN发生均有积极作用。

陈氏黑药膏联合五神汤治疗急性期下肢丹毒的临床观察及对炎症因子的影响

目的:观察陈氏黑药膏联合五神汤治疗急性期下肢丹毒的临床观察及对炎症因子的影响。方法:选择2021年2月~2023年2月在上海市中医医院治未病科接受治疗的85例丹毒患者,采用随机数表法分为试验组(n=43)和对照组(n=42)。两组均基础给予阿莫西林胶囊及五神汤口服,对照组给予金黄膏治疗,试验组给予陈氏黑药膏治疗。1周为1疗程,比较两组临床疗效、白细胞计数(WBC)、中性粒细胞百分比(N%)、C反应蛋白(CRP)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、下肢外周径差、红肿范围、中医证候积分。结果:试验组治愈7例,显效23例,有效11例,无效2例,痊愈率16.28%,痊愈显效率69.77%,总有效率95.34%;对照组治愈3例,显效11例,有效21例,无效7例,痊愈率7.14%,痊愈显效率33.33%,总有效率83.33%。两组比较,试验组痊愈显效率明显优于对照组(P<0.01),试验组与对照组总有效率无明显差异(P>0.05);治疗后,两组白细胞计数、CRP、IL-6、TNF-α均下降,试验组降低效果显著优于对照组(P<0.01);治疗后,两组中性粒细胞百分比均下降,试验组降低效果优于对照组(P<0.05);治疗后,两组下肢外周径差差异无统计学意义(P>0.05),但试验组下降趋势较对照组明显;试验组与对照组均能缩小患者红斑面积,试验组疗效显著优于对照组(P<0.01);两组均能改善中医证候积分,试验组疗效显著优于对照组(P<0.01);治疗期间,对照组不良反应主要有皮疹瘙痒、恶心呕吐、胃肠道不适;试验组不良反应主要有恶心呕吐、胃肠道不适,两组不良反应发生情况比较有统计学意义(P<0.05),试验组不良反应明显少于对照组。结论:在丹毒中应用陈氏黑药膏联合五神汤治疗效果显著,可能与其可有效改善CRP、IL-6及TNF-α水平有关。

陈博来基于“肾为生痰之本”辨治肾虚痰阻型腰痛

肾主水,为先天之本,对于体内津液的运行输布具有重要作用,且肾与水腑膀胱互为表里,通过冲脉与“水谷之海”的胃有经络上的联系,故肾在痰的生成中起着关键作用。陈博来教授认为,肾虚痰阻型腰痛为本虚标实之证,“痰阻”为其标,“肾虚”为其本,临床以腰骶钝痛、痛处固定、腰部僵硬、肢体麻木或偏枯为主要特点,兼见腰膝酸软、喜按喜揉等表现。肾虚痰阻型腰痛的治疗可基于“肾为生痰之本”,以补肾祛痰为法,拟定理痰汤(主要组成药物为芡实、半夏、黑芝麻、柏子仁、白芍、陈皮、茯苓等)为基础方,针对不同兼症辅以健脾、宣肺等药进行加减治疗。其辨治思路可为临床中医治疗腰痛提供参考。

更年春方对卵巢切除大鼠学习记忆能力和海马ER亚型的调节

目的观察更年春方(GNC)对卵巢切除大鼠学习和记忆能力下降的预防和治疗作用,以及GNC对大鼠海马中雌激素核受体(ER)亚型的调节。方法采用10～12月龄SD雌性大鼠切除双侧卵巢建立学习和记忆能力下降的模型,用GNC灌药12周后行Morris水迷宫实验(MWM),实时荧光定量PCR法测定海马ERα和ERβmRNA的变化,免疫组化的方法测定海马CA3区ERα和ERβ蛋白的表达。结果大鼠切除卵巢后,血清E2和MWM成绩下降,海马中ERβmRNA与蛋白的表达均降低,而ERαmRNA和蛋白变化不明显。使用GNC者E2无明显变化,MWM成绩和海马中ERβmRNA与蛋白的表达提高,对ERαmRNA和蛋白无明显影响。结论提示GNC可能通过调节海马中的ERβ改善学习和记忆能力低下。

陈永灿从“本虚毒结”论治慢性萎缩性胃炎伴肠上皮化生经验浅析

[目的]总结陈永灿主任基于“本虚毒结”治疗慢性萎缩性胃炎伴肠上皮化生的经验。[方法]通过随师临诊,收集、整理典型医案,结合陈主任临证的阐释,从核心病机、辨证论治、遣方用药三个方面,总结陈主任临床治疗慢性萎缩性胃炎伴肠上皮化生的经验,并附一则验案予以佐证。[结果]慢性萎缩性胃炎伴肠上皮化生是胃癌发生发展的关键环节,陈主任认为“本虚毒结”是慢性萎缩性胃炎伴肠上皮化生的核心病机,“本虚”即脾胃虚弱,元气不足;“毒结”即浊毒留滞,蕴结隐伏。辨治以寒热错杂为基本面,主张平调寒热,根据虚实偏胜,随证治之。处方以自拟十味泻心汤为主,扶元解毒,如脾虚及肾,加用滋肾之品;兼有气血怫郁,喜用花类疏和;针对痰毒、瘀毒、热毒的不同,分别选用山慈菇、石见穿、猫人参,蜂房、刘寄奴、莪术,蒲公英、半枝莲、白花蛇舌草三对角药治之。验案辨证为脾虚毒结、寒热错杂,治法为健脾解毒、平调寒热,以十味泻心汤为主方,考虑兼有瘀毒阻滞、浊滞血瘀,选用蜂房-刘寄奴-莪术角药;复诊因气血不通,气机升降失调,以三花百草饮轻宣开郁;三诊考虑患者年龄增长,肝肾渐衰,且久病伤元,合四君贞元饮健脾益肾、固本培元。[结论]陈主任从“本虚毒结”辨治慢性萎缩性胃炎伴肠上皮化生经验独到,疗效显著,值得推广学习。

柴芩承气汤对急性胰腺炎大鼠肺泡巨噬细胞PERK基因表达的影响

目的探讨柴芩承气汤(Chai Qin Cheng Qi decoction,CQCQD)对急性胰腺炎(acute pancreatitis,AP)大鼠肺泡巨噬细胞内质网双链RNA依赖蛋白激酶样ER激酶(PKR-like ER kinase,PERK)mRNA表达的影响。方法将30只SD大鼠随机分成对照组、AP组及CQCQD组(n=10)。AP组采用20%L-精氨酸腹腔注射(2.5g·kg-1·1h-1×2)制作;CQCQD组分别于第0、2、4h用CQCQD按0.2mL/100g灌胃,对照组采用生理盐水代替CQCQD灌胃,容积及方法与CQCQD组同。于6h收集胰腺组织行病理检查,肺泡巨噬细胞检测PERK的mRNA表达,血清检测大鼠血清IL-6及TNF-α水平。结果 AP组胰腺组织病理评分(8.8±0.5)分,高于对照组(1.0±0.3)分及CQCQD组(5.4±0.6)分(P<0.05);CQCQD组血清IL-6及TNF-α(48.3±10.7pg/mL,35.1±14.5pg/mL)高于对照组(35.1±10.6pg/mL,21.3±14.2pg/mL,P<0.05),但低于AP组血清(67.5±13.1pg/mL,52.1±13.7pg/mL,P<0.05)。与对照组比较,AP组巨噬细胞PERK mRNA表达上调,CQCQD灌胃可下调其表达(P<0.05)。结论 CQCQD减少炎性因子释放可能与下调巨噬细胞内质网PERK mRNA表达有关。

陈新宇教授分期论治心胀病经验

心胀病是五脏六腑胀病之一,扩张型心肌病可归属于心胀病。陈新宇教授认为心胀病总属本虚标实,心气不足、心阳虚损是发病的关键。临床分初、中、后三期辨证论治,初期心气不足、外邪易犯,中期气虚日久、累及心阳,后期心阳不振、脾肾亏虚。基础方多选用黄芪桂枝五物汤、桂枝茯苓丸、养心汤、真武汤等,灵活加减,治疗效果佳。

陈国良教授藿朴夏苓汤从脾论治慢性乙型肝炎经验

当今随着基层筛查普及和人们观念改变,慢性乙型肝炎的治疗再次成为关注焦点。此文介绍福建省名中医陈国良教授辨治慢性乙型肝炎的相关临证经验。其认为此病内外合邪,外受湿热疫毒,内有正气亏虚,极易迁延反复;病性多属本虚标实,虚实夹杂;强调诊治中应注重辨病与辨证相结合;在临证中以肝郁脾虚兼湿热证多见,陈老基于“从脾论治”,运用藿朴夏苓汤辨治慢性乙型肝炎取得较好疗效。

基于“二阳之病发心脾”从“火”论治早发性卵巢功能不全

中医将早发性卵巢功能不全归为“女子不月”范畴。《黄帝内经》中“二阳之病发心脾,不得隐曲,女子不月”是目前对闭经最早的古籍记载,表明女子不月与二阳、心脾、隐曲等有极大关系。阳明之经燥火煎灼,化生失源,燥热内生,阴血枯涸,气血生化乏源;心脾与经血生成关系密切,火盛燔灼,阴血既被耗又失于化生,心为五脏六腑之大主,火盛于心从而伤及全身,自身统调失司,经血不下,致月经不来;百病皆由气所生,情志失调导致经血不通与不荣,同时情志又与心、肝、脾密切相关,情志过极可致气机升降出入失常,气血生化失调;冲任不盛,胞宫失于濡养,冲任伤于火盛,胞宫精血耗伤,皆可致女性内在生殖激素发生变化,分泌失调,从而卵巢功能下降,窦卵泡数目减少或生长不佳,体现出围绝经期症状,发为闭经,甚至影响女性生殖不孕。文章基于《黄帝内经》原文从“火”来论治女子闭经实证,阐述实火所致闭经的病因病机,以自拟三黄调经汤清阳明、心脾之火,疏情志之郁火,辅以滋阴行血之品,使燥火得清,阴得滋养,血海得充,血道通利,胞宫满盈,经血以时下,从而改善女子闭经及围绝经期症状,改善卵巢功能、激素水平及卵泡质量,为论治早发性卵巢功能不全提供新思路。

陈氏肾复汤对脾肾气虚、湿浊瘀阻型慢性肾脏病3a期患者的疗效观察及对血清胎球蛋白A的影响

目的:观察陈氏肾复汤对脾肾气虚、湿浊瘀阻型慢性肾脏病(Chronic Kidney Disease,CKD)3a期患者的临床疗效,并初步探讨该方防治慢性肾脏病血管钙化的可能性。方法:选取符合入选标准的患者70例,依据简单随机法分为对照组和试验组,每组35例。对照组予以西医常规治疗,试验组在西医常规治疗的基础上加用陈氏肾复汤,疗程均为8周。观察两组治疗前后血肌酐(Serum Creatinine,Scr)、肾小球滤过率(Estimate Glomerular Filtration Rate,eGFR)、24小时尿蛋白定量(Urinary Protein Quantity,UP)、血钙(Ca)、血磷(P)、钙磷乘积(Ca×P)、甲状旁腺激素(Parathyroid Hormone,PTH)、超敏C反应蛋白(Hypersensitive C-reactive Protein,hs-CRP)、血清胎球蛋白A(Fetuin-A)及中医证候积分的变化。结果:与治疗前相比,试验组在降低24 h UP、升高eGFR方面的疗效优于对照组(P <0.05),而在降低Scr方面的疗效与对照组无明显差异(P>0.05);两组患者P、Ca×P、hs-CRP均较治疗前降低(P <0.05),且试验组疗效优于对照组(P <0.05);两组患者Fetuin-A均较治疗前升高(P <0.05),且试验组升高程度优于对照组(P <0.05);两组患者中医证候积分均较治疗前降低(P <0.05),且试验组降低程度优于对照组(P <0.05);试验组的总有效率高于对照组(P <0.05)。结论:在西医常规治疗的基础上加用陈氏肾复汤能更好地改善脾肾气虚、湿浊瘀阻型慢性肾脏病3α期患者的临床症状,减少尿蛋白排泄,保护肾功能,并可通过升高钙化抑制因子血清Fetuin-A水平改善钙磷代谢紊乱和微炎症状态,从而对慢性肾脏病血管钙化起到一定的防治作用。

陈达夫临证运用桂枝汤类方经验

陈达夫教授将伤寒六经理论与家传秘学融合,结合自身诊疗经验,创新性地提出了眼科六经辨证。临床以六经统病,执简驭繁,善用经方治疗眼科疑难疾病,疗效卓绝。文章基于陈达夫教授在眼科六经辨证中,将整体脏腑经络和局部形色血丝结合的特点,凝练出陈达夫教授定六经归属、审兼夹症形、分虚实变化以运用桂枝汤类方的临证思路,旨在总结其在中医眼科领域的经方心法。

畲药十二时辰醇提物外用对大鼠佐剂性关节炎的抗炎作用研究

目的:探讨畲药“十二时辰”醇提物外用对类风湿性关节炎模型大鼠的治疗作用。方法:SD大鼠随机分为正常对照组,模型组,高、低剂量十二时辰治疗组和阳性对照组。采用大鼠右后足趾皮内注射完全弗氏佐剂制作类风湿性关节炎模型,致炎后采用十二时辰醇提液浸泡涂抹方式给药,模型组及阳性对照组分别给予生理盐水或扶他林涂抹,持续给药28 d。于给药前和给药后每7 d测定足趾容积,给药结束,HE染色检测各组大鼠的组织病理学改变,Elisa方法测定血清IL-1β及RF含量。结果:模型组大鼠在各时间点的足趾肿胀度显著高于正常对照组(P<0.05),血清IL-1β及RF含量显著升高(P<0.01),足趾关节病理破坏明显;高剂量十二时辰醇提液外用可显著降低大鼠的足趾肿胀度(P<0.05),降低血清IL-1β及RF含量(P<0.01),改善关节病理损伤。结论:畲药十二时辰醇提液外用可降低佐剂性关节炎大鼠足肿胀,改善病理损伤,降低血清炎症因子IL-1β及RF含量。

石膏煎煮入药方式对小儿麻甘颗粒钙含量影响的研究

目的:建立微波消解前处理结合电感耦合等离子体质谱法(ICP-MS)测定小儿麻甘颗粒钙含量,探讨石膏煎煮入药方式对小儿麻甘颗粒钙含量的影响。方法:建立微波消解前处理结合电感耦合等离子体质谱法(ICP-MS)对制剂中钙的含量进行测定,比较不同粉碎度石膏投料与不同醇沉工艺制备的小试制剂及小儿麻甘颗粒样品中钙含量差异。结果:不同企业间、同企业批间样品钙含量一致性较差;小试制剂结果显示颗粒越大,钙转移率越低;颗粒越小,钙转移率越高。结论:石膏煎煮入药钙的转移率与其粉碎度有关,为保证制剂质量一致性,建议对投料石膏的粉碎度进行统一。本法可为小儿麻甘颗粒生产工艺的优化提供指导意见。

茵陈解毒汤联合非剥脱点阵激光治疗痤疮的效果分析

目的探讨痤疮患者采用茵陈解毒汤联合非剥脱点阵激光治疗的效果。方法选取2022年10月至2023年10月本院收治的痤疮患者共计70例,以治疗方法的不同分成研究组(n=35)与对照组(n=35),对照组采用非剥脱点阵激光治疗,研究组采用茵陈解毒汤联合非剥脱点阵激光治疗,比较两组临床疗效、痤疮改善情况、生活质量。结果统计治疗总有效率,研究组97.14%(34/35)较对照组77.14%(27/35)更高(P<0.05);两组治疗后痤疮综合分级系统量表(Global acne grading system,GAGS)评分下降(P<0.05),研究组(16.67±4.52分)较对照组(19.33±3.91分)更低(P<0.05);两组治疗后卡迪夫痤疮伤残指数(Cardiff Acne Disability Index,CADI)评分下降(P<0.05),研究组(6.11±1.47分)较对照组(8.54±1.76分)更低(P<0.05)。结论茵陈解毒汤联合非剥脱点阵激光治疗痤疮能够提高治疗疗效,改善痤疮情况,提高生活质量。