期刊文献+

二次检索

题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到4,990篇文章
< 1 2 250 >
每页显示 20 50 100
MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis 被引量:1
1
作者 Hong-Xia Bai Xue-Mei Qiu +1 位作者 Chun-Hong Xu Jian-Qiang Guo 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第5期2123-2140,共18页
BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC... BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway. 展开更多
关键词 Gastric cancer MicroRNA-145-5p Serpin family E member 1 Epithelial-mesenchymal transition Proliferation Extracellular signal-regulated kinase-1/2
下载PDF
Exercise-induced modulation of miR-149-5p and MMP9 in LPS-triggered diabetic myoblast ER stress: licorice glycoside E as a potential therapeutic target 被引量:1
2
作者 Yi Du Hong Liu 《Traditional Medicine Research》 2024年第8期23-34,共12页
Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeut... Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions. 展开更多
关键词 ER stress diabetes physical exercise gene expression microRNA-149-5p MMP9 licorice glycoside E traditional physical therapy genomics insights
下载PDF
Research and Practice of the P.E. Information Teaching Design System
3
作者 Xi Jianping 《International English Education Research》 2014年第2期93-96,共4页
In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and t... In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database (nine modules in total) on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency. 展开更多
关键词 Information Teaching Design SYSTEM p.e. Constmctivism
下载PDF
Analysis on the Optimization of the P.E. Course Teaching Plan in Colleges and Universities
4
作者 Dongsheng WANG Zhijun WANG Jian ZUO 《International Journal of Technology Management》 2013年第5期111-113,共3页
In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students' learning efficiency. In this paper, the current situation of the current ... In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students' learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed. 展开更多
关键词 Colleges and Universities p.e. Courses Teaching Plan Suggestions on the Optimization
下载PDF
Field Assessment of the Level of Protection Conferred by a Newly Prepared Combined Inactivated Vaccine against E. coli and P. multocida in Rabbit in Egypt
5
作者 Eman M. El Rawy Wafaa S. Abd El-Moneim +5 位作者 Fatma M. Gad Fatma F. Ibrahim Fatma El Zahraa Gamal Abeer Mwafy Manar F. Seioudy Selim S. Salama 《World Journal of Vaccines》 CAS 2024年第2期43-56,共14页
Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industr... Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industry. Screening for bacterial agents causing mortalities in rabbits revealed the presence of Enterobacteriacae species in approximately 42% of studied cases, with E. coli the most commonly isolated organism. The present study was designed to evaluate the immune response of rabbits vaccinated with a locally prepared, combined inactivated vaccine of Pasteurella multocida and E. coli, adjuvanated with Montanide ISA70. A total of 370 rabbits, aged 2 - 3 weeks, were divided into four groups: (G1) vaccinated with a polyvalent P. multocida vaccine, (G2) vaccinated with a polyvalent E. coli vaccine, (G3) vaccinated with a combined inactivated Montanide ISA70 vaccine of P. multocida and E. coli, and (G4) kept as a non-vaccinated control group. All rabbits received two doses of 0.5 ml of the prepared vaccines, administered one month apart, and were then challenged with virulent strains of P. multocida and E. coli three weeks after the second vaccination. The prepared vaccines were evaluated by determining humoral immunity using indirect haemagglutination (IHA) test and ELISA. The potency of the vaccines was assessed through challenge and determination of LD50. Experimental findings on the prepared polyvalent combined inactivated P. multocida and E. coli vaccine indicated that it is a potent vaccine, producing the highest antibody titers and a 90% protection rate against challenges with virulent strains of P. multocida type A, D2, and E. coli types O157, O151 and O125. Thus, this vaccine is promising in addressing both P. multocida and E. coli problems in rabbits, farms, providing significant protection, and we recommend its commercial production to help rabbit producers control these two major bacterial infections. 展开更多
关键词 P. multocida E. coli Vaccine ELISA Challenge Test Rabbit Protection
下载PDF
关于大学E-teacher的角色思考 被引量:43
6
作者 袁小陆 《外语教学》 CSSCI 北大核心 2006年第3期73-75,共3页
本文从当前大学英语教师的角色困惑出发,探讨了教师角色的含义,提出了E-teacher的概念,阐述了此概念的理论内涵和在实践中,即大学英语教师角色转变中的意义,从而引出对大学英语教师角色转变方向的若干建议。
关键词 大学英语教师 E—teacher 角色 转变
下载PDF
从C.P.E.巴赫的音乐中探寻情感的表现 被引量:3
7
作者 陈家贝 《当代音乐》 2015年第5期105-106,共2页
早在18世纪,作曲家C.P.E.巴赫就在他著名的文献《键盘乐器演奏艺术真谛》中阐述了用纯乐器音乐手法来表现情感,这样的音乐被认为是"感情的语言"。作为一名演奏者,我们从他的思想中进一步剖析和拓展,在演奏的同时力求对作品精... 早在18世纪,作曲家C.P.E.巴赫就在他著名的文献《键盘乐器演奏艺术真谛》中阐述了用纯乐器音乐手法来表现情感,这样的音乐被认为是"感情的语言"。作为一名演奏者,我们从他的思想中进一步剖析和拓展,在演奏的同时力求对作品精神、情感全面地展现。 展开更多
关键词 C.p.e.巴赫 键盘乐器演奏 情感风格
下载PDF
The Prognostic Value of Pathological and Molecular Margins Marked by p53 and eIF4E in Laryngeal Carcinoma
8
作者 夏良平 曾剑 +3 位作者 郭朱明 饶慧兰 曾敬 曾宗渊 《The Chinese-German Journal of Clinical Oncology》 CAS 2005年第1期56-60,69,共6页
Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was s... Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential. 展开更多
关键词 laryngeal neoplasm/squamous cell carcinoma PROGNOSIS molecular margin eukaryotic translation initiation factor 4E P53
下载PDF
Introduction of Internet P.E News Reporter
9
作者 王丽娜 王麟 《科技视界》 2013年第15期18-18,共1页
Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant ... Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information. 展开更多
关键词 P E NEWS INTERNET P E INTRODUCTION
下载PDF
An Analysis of Development as A Reflective Teacher in Higher Education
10
作者 陈利平 鲍勤 《海外英语》 2010年第5X期131-133,共3页
Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analy... Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analysis is to show how effective and important the reflection on the teaching and to show how useful the reflection which can make the teacher change the way of teaching,encouraging the students to adopt a deep approach in their learning. 展开更多
关键词 effective TEACHING REFLECTIVE teacher the 3p model surface LEARNING DEEP LEARNING
下载PDF
Development Characteristics of Private P.E Economy
11
作者 王丽娜 王麟 《科技视界》 2013年第13期30-30,共1页
The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and th... The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy. 展开更多
关键词 P E ECONOMY Leap DEVELOPMENT CHARACTERISTICS
下载PDF
P.E Science J ournals General Influence Factor
12
作者 王丽娜 王麟 《科技视界》 2013年第14期17-17,共1页
The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence ... The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence in daily life, and advocate an academic atmosphere of utilitarianism with a slogan of "article issued online can be published in journal with the recommendation of experts" and practical action. It is the first academic journal under network operation. 展开更多
关键词 P E SCIENCE JOURNALS InInfluence FACTOR
下载PDF
针刺联合核心稳定训练对颈椎病颈痛患者中医症候积分、颈痛程度、生活质量和炎症因子水平的影响
13
作者 许华峰 周伶 +1 位作者 付军杰 隆豫 《检验医学与临床》 2025年第2期242-246,共5页
目的评价针刺联合核心稳定训练对颈椎病颈痛患者中医症候积分、颈痛程度、生活质量和炎症因子水平的影响。方法将2023年1-12月丰都县人民医院收治的80例颈椎病颈痛患者分为对照组(40例)和观察组(40例),对照组予以针刺治疗,观察组予以针... 目的评价针刺联合核心稳定训练对颈椎病颈痛患者中医症候积分、颈痛程度、生活质量和炎症因子水平的影响。方法将2023年1-12月丰都县人民医院收治的80例颈椎病颈痛患者分为对照组(40例)和观察组(40例),对照组予以针刺治疗,观察组予以针刺联合核心稳定训练治疗。比较治疗前后两组中医症候积分(颈肩部疼痛、颈部压痛、头晕、颈部活动受限、肢端麻木疼痛)、颈痛程度、生活质量和炎症因子水平。结果治疗后,两组颈肩部疼痛、颈部压痛、头晕、颈部活动受限、肢端麻木疼痛症状评分均低于治疗前(P<0.05),且治疗后观察组颈肩部疼痛、颈部压痛、头晕、颈部活动受限、肢端麻木疼痛症状评分均低于对照组(P<0.05)。治疗后,两组Northwick Park颈痛量表(NPQ)评分均较治疗前降低(P<0.05),且治疗后观察组NPQ评分低于对照组(P<0.05)。治疗后,两组汉化版简明健康调查量表(SF-36)各维度评分均高于治疗前(P<0.05),且治疗后观察组SF-36各维度评分高于对照组(P<0.05)。治疗后,两组血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)、P物质(SP)水平均较治疗前降低(P<0.05),且治疗后观察组血清IL-6、TNF-α、PGE2、SP水平低于对照组(P<0.05)。结论针刺联合核心稳定训练治疗可有效改善颈椎病颈痛患者的各项中医症候积分,缓解颈痛程度,减轻炎症反应,提高其生活质量。 展开更多
关键词 颈椎病 颈痛 针刺 核心稳定训练 中医症候积分 白细胞介素 肿瘤坏死因子-α 前列腺素E2 P物质
下载PDF
蒙特威尔第“激动风格”和C.P.E.巴赫“激情风格”之比较
14
作者 吴琼 《艺术评鉴》 2019年第10期1-2,共2页
本文选取了文艺复兴晚期和前古典主义时期的两位代表作曲家的创作风格,以作曲家所处的时代背景为依托着重阐述两种不同时代音乐风格的异同,力从对比中着眼看待其对西方音乐发展的影响。
关键词 文艺复兴晚期 前古典主义时期 蒙特威尔第 “激动风格” C.p.e.巴赫 “激情风格”
下载PDF
一部永不休止的流动经典——C.P.E巴赫与他的《钢琴弹奏艺术》 被引量:1
15
作者 张强 《南京艺术学院学报(音乐与表演版)》 2002年第4期37-38,59,共3页
C.P.E.巴赫是“北德乐派”的代表作曲家。作为一个伟大的音乐家,他不仅继承和发展了其父约翰·塞巴斯蒂安·巴赫的音乐创作风格,而且教出了象贝多芬这样的一代钢琴大师。作为一个伟大的作曲家,音乐史上已有记载,而他在钢琴教学... C.P.E.巴赫是“北德乐派”的代表作曲家。作为一个伟大的音乐家,他不仅继承和发展了其父约翰·塞巴斯蒂安·巴赫的音乐创作风格,而且教出了象贝多芬这样的一代钢琴大师。作为一个伟大的作曲家,音乐史上已有记载,而他在钢琴教学上的伟大成就至今少有记述。本文着重研究他在钢琴教学上的贡献。 展开更多
关键词 C.p.e巴雷 《钢琴弹奏艺术》 音乐家
下载PDF
Genetic polymorphisms in cytochrome P4502E1, alcohol and aldehyde dehydrogenases and the risk of esophageal squamous cell carcinoma in Gansu Chinese males 被引量:12
16
作者 Yan-Mei Guo Qin Wang +3 位作者 Yan-Zhen Liu Huei-Min Chen Zhi Qi Qing-Hong Guo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第9期1444-1449,共6页
AIM:To evaluate the association between genetic polymorphisms in CYP2E1, ALDH2 and ADH1B and the risk of esophageal squamous cell carcinoma (ESCC) in a high risk area of Gansu Province, in Chinese males. METHODS: A ca... AIM:To evaluate the association between genetic polymorphisms in CYP2E1, ALDH2 and ADH1B and the risk of esophageal squamous cell carcinoma (ESCC) in a high risk area of Gansu Province, in Chinese males. METHODS: A case-control study was conducted to investigate the genetic polymorphisms of these enzymes (CYP2E1 *c1/*c2, ALDH2 *1/*2 and ADH1B *1/*1 genotypes). A total of 80 esophageal cancer cases and 480 controls were recruited. RESULTS: Compared with controls, cases had a greater prevalence of heavier alcohol consumption (53.8% vs 16.2%) and a higher proportion of alcohol drinkers with > 30 drink-years (28.8% vs 13.5%). Heavier alcohol consumption and alcohol drinking with > 30 drink- years increased the risk of ESCC, with ORs (95% CI) of 3.20 (1.32-9.65) and 1.68 (0.96-3.21). CYP2E1 (*c1/*c1), ALDH2 (*1/*2) and ADH1B (*1/*1) genotype frequencies were higher among patients with squamous cell carcinomas, at a level close to statistical significance (P = 0.014; P = 0.094; P = 0.0001 respectively). There were synergistic interactions among alcohol drinking and ALDH2, ADH1B and CYP2E1 genotypes. The risk of the ESCC in moderate-to-heavy drinkers with an inactive ALDH2 encoded by ALDH2 *1/*2 as well as ADH1B encoded by ADH1B *1/*1 and CYP2E1 encoded by CYP2E1 *c1/*c1 was higher than that in the never/rare-to-light drinkers with an active ALDH2 (*1/*1 genotype) as well as ADH1B (*1/*2 + *2/*2) and CYP2E1 (*c1/*c2 + *c2/*c2) genotypes, with a statistically significant difference; ORs (95% CI) of 8.58 (3.28-22.68), 27.12 (8.52-70.19) and 7.64 (2.82-11.31) respectively. The risk of the ESCC in moderate-to-heavy drinkers with ALDH2 (*1/*2) combined the ADH1B (*1/*1) genotype or ALDH2 (*1/*2) combined the CYP2E1 (*c1/*c1) genotype leads to synergistic interactions, higher than drinkers with ALDH2 (*1/*1) + ADH1B (*1/*2 + *2/*2), ALDH2 (*1/*1) + CYP2E1 (*c1/*c2 + *c2/*c2) respectively , ORs (95% CI) of 7.46 (3.28-18.32) and 6.82 (1.44-9.76) respectively. Individuals with the ADH1B combined the CYP2E1 genotype showed no synergistic interaction. CONCLUSION: In our study, we found that alcohol consumption and polymorphisms in the CYP2E1, ADH1B and ALDH2 genes are important risk factors for ESCC, and that there was a synergistic interaction among polymorphisms in the CYP2E1, ALDH2 and ADH1B genes and heavy alcohol drinking, in Chinese males living in Gansu Province, China. 展开更多
关键词 Esophageal squamous cell carcinoma Cytochromes P4502E1 Alcohol dehydrogenases Aldehyde dehydrogenases Genetic polymorphisms
下载PDF
Late Cretaceous-Early Paleogene Foraminiferal Biostratigraphy in Xishan,Gamba,Southern Tibet,China 被引量:1
17
作者 HAN Yi LI Guobiao +9 位作者 LI Yuewei LI Xinfa WANG Tianyang XIE Dan ZHAO Shengnan ZHAO Jun ZHANG Jialin LI Qi CHEN Yu LI Na 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2019年第S01期106-108,共3页
1 Introduction The Cretaceous/Tertiary(Paleogene)extinction event was a mass extinction event occurring at about 65 million years ago between the Mesozoic Cretaceous and Cenozoic Tertiary,which is the one closest to t... 1 Introduction The Cretaceous/Tertiary(Paleogene)extinction event was a mass extinction event occurring at about 65 million years ago between the Mesozoic Cretaceous and Cenozoic Tertiary,which is the one closest to today among the five major extinction events in the the geological history period(Renne et al.,2013).At the end of the Cretaceous,the proportion of species declined gradually. 展开更多
关键词 FORAMINIFERA BIOSTRATIGRAPHY K/T(Pg)boundary P/E BOUNDARY Gamba Tibet
下载PDF
Evaluation in vinyl chloride monomer-exposed workers and the relationship between liver lesions and gene polymorphisms of metabolic enzymes 被引量:6
18
作者 Shou-Min Zhu Xue-Feng Ren Jun-Xiang Wan Zhao-Lin Xia 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第37期5821-5827,共7页
AIM: To analyze occupational health hazards exposure to doses lower than the Chinese occupational health standard in a selected VC polymerization plant in China, and also to elucidate the relationship between genetic... AIM: To analyze occupational health hazards exposure to doses lower than the Chinese occupational health standard in a selected VC polymerization plant in China, and also to elucidate the relationship between genetic polymorphisms and genetic susceptibility on liver lesions of workers exposed to vinyl chloride monomer (VCM). METHODS: In order to explore the mechanism of VCM- related health effects, we used a case-control design to investigate the association between the genetic polymorphisms of metabolic enzymes and liver lesions in workers occupationally exposed to VCM. Genotypes of CYP2E1, GSTT1, GSTM1, ALDH2 and ADH2 were identified using PCR and PCR-RFLP. RESULTS: Even when the concentration of VCM was lower than the current Chinese occupational health standard, neurasthenia, pharyngeal irritation, liver ultrasonography abnormalities and hemoglobin disorders were significantly higher in exposure subjects compared to non-exposure subjects, and the relative risks (RRand 95% C1) were 1.74 (1.06-2.85), 1.97 (1.56-2.48), 10.69 (4.38-26.12), and 2.07 (1.20-3.57). CYP2E1 c1c2/c2c2 genotype was significantly associated with liver damages (OR 3.29, 95% CI 1.51-7.20, P〈0.01). CONCLUSION: The incidences of neurasthenia and liver ultrasonography abnormalities significantly increase when the cumulative exposure dose increases. The genotypes of metabolic enzymes (CYP2E1 c1c2/c2c2, null GSTT1 and ADH2 1-1) play important roles in VCM metabolism. Polymorphisms of CYP 2E1, GSTT1 and ADH2 may be a major reason of genetic susceptibility in VCM-induced hepatic damage. 展开更多
关键词 Vinyl chloride monomer Hepatic lesions Cytochrome p450 2E1 Glutathione S-transferase Aldehyde dehydrogenase-2
下载PDF
Detection of CYP2E1,a Genetic Biomarker of Susceptibility to Benzene Metabolism Toxicity in Immortal Human Lymphocytes Derived from the Han Chinese Population 被引量:4
19
作者 ZHANG Juan YIN LiHong LIANG GeYu LIU Ran FAN KaiHong PU YuePu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2011年第3期300-309,共10页
Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia, We aimed to identify the CY... Objective Cytochrome P450 2E1 (CYP2E1) is an important metabolizing enzyme involved in oxidative stress responses to benzene, a chemical associated with bone marrow toxicity and leukemia, We aimed to identify the CYP2E1 genetic biomarkers of susceptibility to benzene toxicity in support of environmental and occupational exposure prevention, and to test whether a model using immortal human lymphocytes might be an efficient tool for detecting genetic biomarkers. Methods Immortalized human lymphocyte cell lines with independent genotypes on four CYP2E1 SNP sites were induced with 0.01% phenol, a metabolite of benzene. CYP2E1 gene function was evaluated by mRNA expression and enzyme activity. DNA damage was measured by Single-Cell Gel Electrophoresis (SCGE). Results Among the four SNPs, cells with rs2070673TT and rs2030920CC showed higher levels of ~YP2E1 transcription and enzymatic activity than the other genotypes in the same SNP site. Cells with higher gene expression genotypes also showed higher comet rates compared with lower gene expression genotypes. Conclusion These results suggest that CYP2E1 rs2070673 and rs2030920 might be the genetic biomarkers of susceptibility to benzene toxicity and that the immortalized human lymphocytes model might be an efficient tool for the detection of genetic biomarkers of susceptibility to chemicals. 展开更多
关键词 Cytochrome P450 2E1 Single-nucleotide polymorphism Genetic biomarker Human immortalized B lymphocytes BENZENE Phenol
下载PDF
Expression of p53, p21^(CIP1/WAF1) and eIF4E in the adjacent tissues of oral squamous cell carcinoma:establishing the molecular boundary and a cancer progression model 被引量:1
20
作者 Yi Li Bo Li +4 位作者 Bo Xu Bo Han Hui Xia Qian-Ming Chen Long-Jiang Li 《International Journal of Oral Science》 SCIE CAS CSCD 2015年第3期161-168,共8页
The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma(OSCC) and adjacent tissues to establish a molecular surgical boundary and provide a cancer progr... The present study evaluated the expression of key molecules and the status of DNA in both oral squamous cell carcinoma(OSCC) and adjacent tissues to establish a molecular surgical boundary and provide a cancer progression model. Biopsy samples from 50 OSCC patients were divided into T(cancer), P1(0–0.5 cm), P2(0.5–1 cm), P3(1–1.5 cm) and P4(1.5–2 cm) groups based on the distances from the visible boundary of the primary focus. Twenty samples of normal mucosa were used as controls. We used immunohistochemical staining and flow cytometry to evaluate p53, p21CIP1/WAF1, e IF4 E and Ki-67 expression and to determine DNA status, respectively. Sub-mucosal invasion was present in the P1 and P2 groups as determined by haematoxylin and eosin staining.Mutant p53 expression decreased gradually from cancerous to normal mucosae, whereas p21CIP1/WAF1 expression displayed an opposite trend. e IF4 E expression decreased from cancerous to normal mucosae. Ki-67 expression, the heteroploidy ratio, S-phase fraction and proliferative index decreased gradually with the distance from the tumour centre. Based on these results, we suggest that the resection boundary in OSCC surgery should be beyond 2 cm from the tumour. Additionally, the adjacent tissues of the primary focus could be used as a model for assessing cancer progression. 展开更多
关键词 e IF4E molecular boundary oral squamous cell carcinoma P21 P53
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部