Stimulated emission depletion(STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the ...Stimulated emission depletion(STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the specially engineered beam profile of the depletion beam and its power. However, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen's optical properties, resulting in a compromised spatial resolution. The situation gets deteriorated when thick samples are imaged. In the worst case, the severe distortion of the depletion beam profile may cause complete loss of the superresolution effect no matter how much depletion power is applied to specimens. Previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. However, it is difficult to correct the complicated high-order optical aberrations of specimens. In this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. The full correction can effectively maintain and improve spatial resolution in imaging thick samples.展开更多
Distributed optical fiber sensors have been widely used to monitor temperature, strain, vibration, and so on. Specifically, the sensors based on Brillouin scattering have been studied extensively to measure the strain...Distributed optical fiber sensors have been widely used to monitor temperature, strain, vibration, and so on. Specifically, the sensors based on Brillouin scattering have been studied extensively to measure the strain or temperature along an oDtical fiber.展开更多
基金National Basic Research Program of China(2015CB352005)National Natural Science Foundation of China(NSFC)(61378091,61404123,61505118,61505121,61525503)+5 种基金China Postdoctoral Science Foundation(2014M55226)Natural Science Foundation of Guangdong Province(2014A030312008)Hong Kong,Macao and Taiwan cooperation innovation platform&major projects of international cooperation in Colleges and Universities in Guangdong Province(2015KGJHZ002)National Institute of General Medical Sciences(NIGMS)(P20GM103499,R21GM104683)National Science Foundation(NSF)(1539034)Shenzhen Basic Research Project(JCYJ20150930104948169,GJHZ20160226202139185,JCYJ20160328144746940)
文摘Stimulated emission depletion(STED) microscopy is one of far-field optical microscopy techniques that can provide sub-diffraction spatial resolution. The spatial resolution of the STED microscopy is determined by the specially engineered beam profile of the depletion beam and its power. However, the beam profile of the depletion beam may be distorted due to aberrations of optical systems and inhomogeneity of a specimen's optical properties, resulting in a compromised spatial resolution. The situation gets deteriorated when thick samples are imaged. In the worst case, the severe distortion of the depletion beam profile may cause complete loss of the superresolution effect no matter how much depletion power is applied to specimens. Previously several adaptive optics approaches have been explored to compensate aberrations of systems and specimens. However, it is difficult to correct the complicated high-order optical aberrations of specimens. In this report, we demonstrate that the complicated distorted wavefront from a thick phantom sample can be measured by using the coherent optical adaptive technique. The full correction can effectively maintain and improve spatial resolution in imaging thick samples.
基金supported by the National Natural Science Foundation of China(Nos.61377089 and 61527819)the International Science&Technology Cooperation Program of China(No.2014DFA50870)the Natural Science Foundation of Shanxi(Nos.2015011049 and201601D021069)
文摘Distributed optical fiber sensors have been widely used to monitor temperature, strain, vibration, and so on. Specifically, the sensors based on Brillouin scattering have been studied extensively to measure the strain or temperature along an oDtical fiber.