BACKGROUND Heat-clearing and detoxifying drugs has protective effect on colorectal cancer(CRC).Given the complicated features of Traditional Chinese medicine formulas,network pharmacology is an effective approach for ...BACKGROUND Heat-clearing and detoxifying drugs has protective effect on colorectal cancer(CRC).Given the complicated features of Traditional Chinese medicine formulas,network pharmacology is an effective approach for studying the multiple interactions between drugs and diseases.AIM To systematically explore the anticancer mechanism of heat-clearing and detoxifying drug JC724.METHODS This study obtained the active compounds and their targets in JC724 from Traditional Chinese Medicine System Pharmacology Database.In addition,the CRC targets were obtained from Drugbank,TTD,DisGeNET and GeneCards databases.We performed transcriptome analysis of differentially expressed genes in CRC treated with JC724.Venn diagram was used to screen the JC724-CRC intersection targets as candidate targets.Core targets were selected by proteinprotein interaction network and herb ingredient-target-disease network analysis.The functional and pathway of core targets were analysed by enrichment analysis.RESULTS We found 174 active ingredients and 283 compound targets from JC724.940 CRC-related targets were reserved from the four databases and 304 CRC differentially expressed genes were obtained by transcriptome analysis.We constructed the network and found that the five core ingredients were quercetin,βBeta sitosterol,wogonin,kaempferol and baicalein.The core JC724-CRC targets were CYP1A1,HMOX1,CXCL8,NQO1 and FOSL1.JC724 acts on multiple signaling pathways associated with CRC,including the Nrf2 signaling pathway,oxidative stress,and the IL-17 signaling pathway.CONCLUSION In this study,we systematically analyzed the active ingredients,core targets and main mechanisms of JC724 in the treatment of CRC.This study could bring a new perspective to the heat-clearing and detoxifying therapy of CRC.展开更多
目的:探究温阳化瘀解毒法治疗脓毒症心肌抑制的临床效果。方法:选取本院ICU收治的脓毒症心肌损伤患者60例为研究对象,按照随机数字表法分为对照组(n=30例)和观察组(n=30例)。两组患者均给予西医常规治疗,对照组患者在常规处理基础上给...目的:探究温阳化瘀解毒法治疗脓毒症心肌抑制的临床效果。方法:选取本院ICU收治的脓毒症心肌损伤患者60例为研究对象,按照随机数字表法分为对照组(n=30例)和观察组(n=30例)。两组患者均给予西医常规治疗,对照组患者在常规处理基础上给予多巴酚丁胺静脉泵入治疗;观察组患者则在对照组基础上采用中医温阳化瘀解毒法治之,给予温阳化瘀解毒汤内服,疗程均为7d。通过中医症状积分变化评价温阳化瘀解毒法治疗脓毒症心肌抑制疗效,观察治疗前后心肌功能指标变化,监测心肌抑制因子内皮素-1(endothelin-1,ET-1)、白三烯(leukotrienes,LT)、高迁移率族蛋白B-1(high mobility group protein B-1,HMBG-1)水平,统计两组患者治疗前后的APACHE II评分、ICU住院时间及ICU内病死率。结果:治疗后,观察组患者的临床症状改善更为明显,其中医证候积分(8.12±3.04)分较对照组(11.75±4.31)分明显降低(P<0.05)。两组患者的心肌功能指标LVESD、LVEDD、BNP、cTnI2较治疗前明显降低,LVEF、CI水平则较治疗前明显升高(P<0.05);治疗后组间对比,观察组患者的LVESD、LVEDD、BNP、cTnI2水平明显低于对照组,而LVEF、CI水平则明显高于对照组(P<0.05);两组患者的心肌抑制因子ET-1、LT、HMBG-1水平均较同组治疗前下降,以观察组患者的水平更低(P<0.05);治疗后,观察组患者的APACHEII评分(13.52±3.29)分明显低于对照组的(20.45±5.37)分,其ICU住院时间(6.29±2.59)d较对照组的(12.14±4.35)d明显缩短,ICU病死率23.33%较对照组的43.33%更低(P<0.05)。结论:温阳化瘀解毒法利于改善脓毒症心肌抑制患者的中医证候,提高心肌收缩功能,改善患者的心功能,降低了心肌抑制因子水平,减轻心肌损害,缩短ICU住院时间,降低病死率,值得临床推广运用。展开更多
基金Supported by The National Natural Science Foundation of China,No.82074061Beijing Natural Science Foundation Proposed Program,No.7202076.
文摘BACKGROUND Heat-clearing and detoxifying drugs has protective effect on colorectal cancer(CRC).Given the complicated features of Traditional Chinese medicine formulas,network pharmacology is an effective approach for studying the multiple interactions between drugs and diseases.AIM To systematically explore the anticancer mechanism of heat-clearing and detoxifying drug JC724.METHODS This study obtained the active compounds and their targets in JC724 from Traditional Chinese Medicine System Pharmacology Database.In addition,the CRC targets were obtained from Drugbank,TTD,DisGeNET and GeneCards databases.We performed transcriptome analysis of differentially expressed genes in CRC treated with JC724.Venn diagram was used to screen the JC724-CRC intersection targets as candidate targets.Core targets were selected by proteinprotein interaction network and herb ingredient-target-disease network analysis.The functional and pathway of core targets were analysed by enrichment analysis.RESULTS We found 174 active ingredients and 283 compound targets from JC724.940 CRC-related targets were reserved from the four databases and 304 CRC differentially expressed genes were obtained by transcriptome analysis.We constructed the network and found that the five core ingredients were quercetin,βBeta sitosterol,wogonin,kaempferol and baicalein.The core JC724-CRC targets were CYP1A1,HMOX1,CXCL8,NQO1 and FOSL1.JC724 acts on multiple signaling pathways associated with CRC,including the Nrf2 signaling pathway,oxidative stress,and the IL-17 signaling pathway.CONCLUSION In this study,we systematically analyzed the active ingredients,core targets and main mechanisms of JC724 in the treatment of CRC.This study could bring a new perspective to the heat-clearing and detoxifying therapy of CRC.
文摘目的:探究温阳化瘀解毒法治疗脓毒症心肌抑制的临床效果。方法:选取本院ICU收治的脓毒症心肌损伤患者60例为研究对象,按照随机数字表法分为对照组(n=30例)和观察组(n=30例)。两组患者均给予西医常规治疗,对照组患者在常规处理基础上给予多巴酚丁胺静脉泵入治疗;观察组患者则在对照组基础上采用中医温阳化瘀解毒法治之,给予温阳化瘀解毒汤内服,疗程均为7d。通过中医症状积分变化评价温阳化瘀解毒法治疗脓毒症心肌抑制疗效,观察治疗前后心肌功能指标变化,监测心肌抑制因子内皮素-1(endothelin-1,ET-1)、白三烯(leukotrienes,LT)、高迁移率族蛋白B-1(high mobility group protein B-1,HMBG-1)水平,统计两组患者治疗前后的APACHE II评分、ICU住院时间及ICU内病死率。结果:治疗后,观察组患者的临床症状改善更为明显,其中医证候积分(8.12±3.04)分较对照组(11.75±4.31)分明显降低(P<0.05)。两组患者的心肌功能指标LVESD、LVEDD、BNP、cTnI2较治疗前明显降低,LVEF、CI水平则较治疗前明显升高(P<0.05);治疗后组间对比,观察组患者的LVESD、LVEDD、BNP、cTnI2水平明显低于对照组,而LVEF、CI水平则明显高于对照组(P<0.05);两组患者的心肌抑制因子ET-1、LT、HMBG-1水平均较同组治疗前下降,以观察组患者的水平更低(P<0.05);治疗后,观察组患者的APACHEII评分(13.52±3.29)分明显低于对照组的(20.45±5.37)分,其ICU住院时间(6.29±2.59)d较对照组的(12.14±4.35)d明显缩短,ICU病死率23.33%较对照组的43.33%更低(P<0.05)。结论:温阳化瘀解毒法利于改善脓毒症心肌抑制患者的中医证候,提高心肌收缩功能,改善患者的心功能,降低了心肌抑制因子水平,减轻心肌损害,缩短ICU住院时间,降低病死率,值得临床推广运用。