Hollow-fiber liquid-phase microextraction(HF-LPME)and electromembrane extraction(EME)are miniaturized extraction techniques,and have been coupled with various analytical instruments for trace analysis of heavy metals,...Hollow-fiber liquid-phase microextraction(HF-LPME)and electromembrane extraction(EME)are miniaturized extraction techniques,and have been coupled with various analytical instruments for trace analysis of heavy metals,drugs and other organic compounds,in recent years.HF-LPME and EME provide high selectivity,efficient sample cleanup and enrichment,and reduce the consumption of organic sol-vents to a few micro-liters per sample.HF-LPME and EME are compatible with different analytical in-struments for chromatography,electrophoresis,atomic spectroscopy,mass spectrometry,and electrochemical detection.HF-LPME and EME have gained significant popularity during the recent years.This review focuses on hollow fiber based techniques(especially HF-LPME and EME)of heavy metals and pharmaceuticals(published 2017 to May 2019),and their combinations with atomic spectroscopy,UV-VIS spectrophotometry,high performance liquid chromatography,gas chromatography,capillary elec-trophoresis,and voltammetry.展开更多
This paper describes flow visualization techniques employing surface oil flow and liquid crystal thermography suitable for use in impulse wind tunnels.High spatial resolution photographs of oil flow patterns and liqui...This paper describes flow visualization techniques employing surface oil flow and liquid crystal thermography suitable for use in impulse wind tunnels.High spatial resolution photographs of oil flow patterns and liquid crystal thermograms have been obtained within test times ranging from 7 to 500 ms and have been shown to be very useful for revealing the detailed features of 3-D separated flow.The results from oil flow patterns,liquid crystal thermograms,schlieren photographs and heat flux measurements are shown to be in good agreement.展开更多
Pickling waste liquid of the steel industry is included in National Hazardous Wastes Catalogue because of its high corrosion and large discharge. The techniques for treating pickling waste liquid ( H2SO4 ) in steel ...Pickling waste liquid of the steel industry is included in National Hazardous Wastes Catalogue because of its high corrosion and large discharge. The techniques for treating pickling waste liquid ( H2SO4 ) in steel industry is summarized. The advantages, disadvantages and applied ranges of all kinds of control technologies are comparatively analysed. The research trends of techniques for treating pickling waste liquid ( H2SO4 ) are the resource reclamation technologies in future which is pointed out in the paper.展开更多
Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart di...Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results:rHPLC is more convenient, sensitive,specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.展开更多
<b><span>Background:</span></b><span> Liquid-based cytology (LBC) is a method of manufacturing cyto-diagnostic specimens. Improved accuracy is expected from standardizing specim...<b><span>Background:</span></b><span> Liquid-based cytology (LBC) is a method of manufacturing cyto-diagnostic specimens. Improved accuracy is expected from standardizing specimen production and use of this method is rapidly spreading in oral cytology. On the other hand, LBC reportedly requires training to show peculiar cell findings compared to those of conventional smear cytology (CVC). Few studies have compared detailed cell findings for oral CVC and LBC.</span><span> </span><b><span>Objec</span></b><b><span>tives:</span></b><span> The aim of this study was to compare cytological findings between CVC </span><span>and LBC using cytomorphological image analysis.</span><span> </span><b><span>Materials and Methods:</span></b><span> Cytological specimens were collected from 20 patients (negative for squamous neoplasia in 10, dysplasia in 5, squamous cell carcinoma in 5) and 5 controls of the tongue between January 2017 and December 2018. Two different preparation techniques were investigated cytomorphologically for CVC and LBC (BD Cytorich</span><sup><span style="vertical-align:super;">TM</span></sup><span>).</span><span> </span><b><span>Results:</span></b><span> LBC showed significantly higher cell numbers tha</span><span>n CVC for all lesions. LBC-to-CVC ratio ranged from 9.52 (hyp</span><span>erkeratosis) to 1.87 (deep cells in oral squamous cell carcinoma (OSCC)). Nuclear area of </span><span>normal, hyperkeratosis, and inflammation were significantly higher in LBC tha</span><span>n those of CVC. Hyperchromasia was significantly more frequent with </span><span>CVC than with LBC for hyperkeratosis, inflammation, dysplasia and OSCC. There was no significant difference in circularity between CVC and LBC </span><span>among all lesions. </span><b><span>Conclusion:</span></b><span> Only one cytomorphological disadvantage was </span><span>se</span><span>en with LBC, in the form of decreased hyperchromasia. Further </span><span>clarification of the advantages and disadvantages of LBC is needed, including management of precision and screening practices.展开更多
Cancer of the corpus uteri remains the most common gynecological related cancer in developed countries. Cytology, after the induction of liquid based cytology, has reemerged as a possible first line non-interventional...Cancer of the corpus uteri remains the most common gynecological related cancer in developed countries. Cytology, after the induction of liquid based cytology, has reemerged as a possible first line non-interventional diagnostic procedure with promising results. Apart from slide preparation for cytology diagnosis, LBC allows the application of elaborate molecular tests on the residual material. Samples from 74 symptomatic women were collected in ThinPrep?PreservCyt medium, from witch immunocytochemical and molecular tests were performed. Final diagnosis of 39 endometrioid carcinomas, 20 non-endometrioid carcinomas and 15 non-malignant was set after hysterectomy. Topoisomerase IIa expression was common (42%) in both types of cancer. Promoter methylation analysis revealed that hMLH1 is commonly methylated in cancers (52.7%), CDKN2A and MGMT less often (27.1%) and RARB rarely methylated (8.4%). BRAF activating mutation V600E was a rare event (8.4%) only found in low grade endometrioid carcinomas. Topoisomerase IIa expression correlated with BRAF mutations, hMLH1 and to lesser extent with CDKN2A methylation. Almost none of the biomarkers were positive in cytological negative or hyperplastic without atypia samples. Detection of methylation in any gene displayed sensitivity, specificity, PPV and NPV similar to cytology of cancer. However, inclusion of cytology diagnosis of hyperlasias with atypia increased sensitivity and NPV of cytology outperforming methylation of any gene. Further evaluation of the panel of promoter methylation, especially in cytology diagnoses of hyperplasia with or without atypia should be evaluated since initial results are promising. Even though methylation of MGMT and RARB are rare events, some patients could be benefit from specific chemotherapeutics that target either of them or the more frequently expressed topoisomerase IIa.展开更多
This study examined cells contained in needles used for the collection of breast fine needle aspirates for the detection of malignant cells trapped in the needles. Remnants of cells contained in 50 needles used for th...This study examined cells contained in needles used for the collection of breast fine needle aspirates for the detection of malignant cells trapped in the needles. Remnants of cells contained in 50 needles used for the collection of scanty breast fine needle aspirates were examined by the liquid based cytology technique and compared with the conventional cytological technique of specimens in the corresponding syringes. The breast specimens were collected with clean sterile needles attached to the syringes. Smears were made and stained by the conventional method. The needles were removed from the syringes and a fixative was withdrawn into the syringes and the syringes were recapped with the needles. The fixative containing the specimen was then completely discharged into a centrifuge tube through the needles and treated by the liquid based cytology technique. The study revealed that cells were found trapped in all the needles used for the collection of breast FNA. 6% of them were positive for malignancy, similar to results obtained in the conventional method. Needles used for the collection of breast FNA should be examined before malignancy is completely ruled out particularly in extremely scanty specimens with a clinical suspicion of malignancy.展开更多
This review gives a short introduction into the principles of ultrasonic measurement techniques for liquids, using cavity resonators. Guidelines for the resonator design in broad-band ultrasonic stxctroscopy as well a...This review gives a short introduction into the principles of ultrasonic measurement techniques for liquids, using cavity resonators. Guidelines for the resonator design in broad-band ultrasonic stxctroscopy as well as in high-resolution single-frequncy or narrowband applications are presented. Deviations of the field configuration in real cells frtxn that in an ideal resonator are discussed and relations for the mode spectrum of cavity fields are given. Recent resonator measurement procedures and methods of data evaluation are mentioned briefly. Some examples of measurements show the extended usability of ultrasonic resonator techniques in basic science and in a wide range of applications for rrkaterials characterization, in manufacturing processes, as well as in control routines.展开更多
Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hos...Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hospital das Clínicas of Faculty of Medicine, Sao Paulo State University. Materials and Methods: We evaluated LBC samples from women referred to gynecology examination for different reasons (previous abnormal PapTest, follow up of treated cervical lesion, ecc) and women examined for regular screening proposals, and compared with biopsy diagnoses, including the controversial diagnoses of vaginal intraepithelial neoplasia (VAIN). Results: From 1866 patients, 1423 (76.3%) cases were negative and 443 (23.7%) were positive for any cellular alteration. Age of patients ranged from 12 to 86 years. We detected 25 histologically confirmed VAIN (1.3% p = 0.0002 by Fisher’s exact test IC 95% 0.0090 - 0.0198) and 1.1% VAIN (p = 0.0031 by Fisher’s exact test IC 95% 0.0077 - 0.0179). Conclusion: The identification of VAIN in routine is feasible;the professionals involved with cytological examination should be aware of these lesions in Pap test samples.展开更多
Immiscible alloys have attracted growing interest for their valuable physical and mechanical properties. However, their production is difficult because of metallurgical problems in which there is a serious tendency fo...Immiscible alloys have attracted growing interest for their valuable physical and mechanical properties. However, their production is difficult because of metallurgical problems in which there is a serious tendency for gravity separation in the region of the miscibility gap. So far the study on the liquid separation mechanism is still one of the important projects in the spatial materials science and the spatial fluid science. The studied results about the liquid phase separating mechanism of immiscible alloys are presented, at the same time the preparation techniques of homogeneous immiscible alloys are summarized, and the existing problems and the related researching areas in the future are also pointed out.展开更多
[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotramete...[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotrametenolic acid and dehydroeburicoic acid as indicators,differences in dissolution were compared between liquid-solid compressed tablets and crude drug of total triterpenoids in Poriae Cutis;crude drug,powder of liquid-solid compressed tablets and excipients of liquid-solid compressed tablets were characterized by differential scanning calorimetry( DSC).[Results]Liquid-solid compact technique could significantly improve dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid. The total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets was 92%,t50( time for 50% total dissolution rate) and t_D( time for 63. 2% total dissolution rate) were 11. 18 min and 22. 71 min; the total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in crude drug of total triterpenoids was 29%,and t50 and t_D were231. 06 min and 359. 23 min. DSC showed that there was no mutual interaction between excipients and total triterpenoids in Poriae Cutis; on the DSC curve for powder of liquid-solid compressed tablets,the absorption peak vanished completely,indicating that the drug exists in the amorphous form in the liquid-solid powder.[Conclusions] Liquid-solid compact technique can increase the dissolution rate of total triterpenoids in Poriae Cutis and allow rapid release of poorly water soluble drugs.展开更多
AIM To evaluate the cytological diagnostic capacity and sample quality of the slow-pull technique and compare them with different suction techniques.METHODS From July 2010 to December 2015, 102 patients with pancreati...AIM To evaluate the cytological diagnostic capacity and sample quality of the slow-pull technique and compare them with different suction techniques.METHODS From July 2010 to December 2015, 102 patients with pancreatic solid lesions who underwent endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA) with 22-gauge needles were retrospectively evaluated. EUS-FNA diagnosis was based on a cytological examination, and final diagnosis was based on a comprehensive standard of cytological diagnosis, surgical pathology and clinical or imaging follow-up. Cytological specimens were characterized for cellularity and blood contamination. The cytological diagnostic capacity and sample quality of the slow-pull technique and suction techniques with 5-m L/10-m L/20-m L syringes were analyzed.RESULTS Of all of the EUS-FNA procedures, the slow-pull technique and suction techniques with 5-m L/10-m L/20-m L syringes were used in 31, 19, 34 and 18 procedures, respectively. There were significant differences between these four suction techniques in terms of cytological diagnostic accuracy(90.3% vs 63.2% vs 58.8% vs 55.6%, P = 0.019), sensitivity(88.2% vs 41.7% vs 40.0% vs 36.4%, P = 0.009) and blood contamination(score ≥ 2 for 29.0% vs 52.6% vs 70.6% vs 72.2%, P = 0.003). The accuracy and sensitivity of the slow-pull technique were significantly higher than those of the suction techniques using 5-m L(P = 0.03, P = 0.014), 10-m L(P = 0.005; P = 0.006) and 20-mL syringes(P = 0.01, P = 0.01). Blood contamination was significantly lower in the slow-pull technique than in the suction techniques with 10-m L(P = 0.001) and 20-mL syringes(P = 0.007).CONCLUSION The slow-pull technique may increase the cytological diagnostic accuracy and sensitivity with slight blood contamination during EUS-FNA when using 22-gauge needles for solid pancreatic masses.展开更多
Nanometer powders can be produced by an electrohydrodynamic technique. The breakup mechanism of the metal droplets generated by the electrohydrodynamic technique was analysed. It showed that the applied voltage, the e...Nanometer powders can be produced by an electrohydrodynamic technique. The breakup mechanism of the metal droplets generated by the electrohydrodynamic technique was analysed. It showed that the applied voltage, the electric field Btrength, the properties of the molten metal and the volumetric flow rate of the molten metal directly effect the breat-up extent of the metal droplets.展开更多
目的探索并验证基于华为云ModelArts平台构建的深度学习模型在宫颈液基细胞学(liquid-based cytology,LBC)非典型细胞诊断中的应用价值,并评估其对不同诊断经验医师的辅助效果。方法回顾性分析2020年东莞市人民医院1044例宫颈脱落细胞...目的探索并验证基于华为云ModelArts平台构建的深度学习模型在宫颈液基细胞学(liquid-based cytology,LBC)非典型细胞诊断中的应用价值,并评估其对不同诊断经验医师的辅助效果。方法回顾性分析2020年东莞市人民医院1044例宫颈脱落细胞学标本,采用华为云ModelArts平台开发的人工智能(artifical intelligence,AI)辅助诊断系统与初级、中级、高级医师进行诊断比对,计算灵敏度、特异度、精确率、符合率、曲线下面积(area under the curve,AUC)等指标,评估AI系统的诊断效能及其对不同年资医师的辅助诊断效果。采用McNemar检验比较AI系统与人工诊断的差异。结果在1044例宫颈脱落细胞学标本中,AI系统在非典型细胞检出的灵敏度和特异度分别为98.96%和89.15%,均高于初级医师(81.95%和91.81%)。AI系统的总体诊断精确率为93.68%,显著高于初级医师(87.26%,P<0.001)。AI辅助可显著提高初级医师的诊断性能,灵敏度从80.1%提升至96.5%,特异度从85.6%提升至92.3%。结论本研究构建的AI辅助宫颈细胞学诊断系统性能优越,尤其能显著提高初级医师的诊断水平,具有良好的临床应用前景。展开更多
基金supported by the Higher education commission of Pakistan(NRPU No.20-3925/R&D/NRPU/HEC/2014)PAK-US science and technology cooperation(Pak-US No6-4/PAK-US/HEC/2015/04)Pakistan science foundation joint research projects with MSRT,Iran(No.PSF-MSRT/Env/KP-AWKUM)。
文摘Hollow-fiber liquid-phase microextraction(HF-LPME)and electromembrane extraction(EME)are miniaturized extraction techniques,and have been coupled with various analytical instruments for trace analysis of heavy metals,drugs and other organic compounds,in recent years.HF-LPME and EME provide high selectivity,efficient sample cleanup and enrichment,and reduce the consumption of organic sol-vents to a few micro-liters per sample.HF-LPME and EME are compatible with different analytical in-struments for chromatography,electrophoresis,atomic spectroscopy,mass spectrometry,and electrochemical detection.HF-LPME and EME have gained significant popularity during the recent years.This review focuses on hollow fiber based techniques(especially HF-LPME and EME)of heavy metals and pharmaceuticals(published 2017 to May 2019),and their combinations with atomic spectroscopy,UV-VIS spectrophotometry,high performance liquid chromatography,gas chromatography,capillary elec-trophoresis,and voltammetry.
文摘This paper describes flow visualization techniques employing surface oil flow and liquid crystal thermography suitable for use in impulse wind tunnels.High spatial resolution photographs of oil flow patterns and liquid crystal thermograms have been obtained within test times ranging from 7 to 500 ms and have been shown to be very useful for revealing the detailed features of 3-D separated flow.The results from oil flow patterns,liquid crystal thermograms,schlieren photographs and heat flux measurements are shown to be in good agreement.
基金Supported by Open Fund Project of Key Laboratory of Environmenta Engineering in Jiangsu,China( KF2012006)Undergraduate Practice Innovation Training Plan Project of Jiangsu Colleges and Universities, China ( Sujiaogao[2011]10)Research Fund Project of Nanjing College of Chemical Technology in 2012,China( NHKY-2012-06)
文摘Pickling waste liquid of the steel industry is included in National Hazardous Wastes Catalogue because of its high corrosion and large discharge. The techniques for treating pickling waste liquid ( H2SO4 ) in steel industry is summarized. The advantages, disadvantages and applied ranges of all kinds of control technologies are comparatively analysed. The research trends of techniques for treating pickling waste liquid ( H2SO4 ) are the resource reclamation technologies in future which is pointed out in the paper.
文摘Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results:rHPLC is more convenient, sensitive,specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.
文摘<b><span>Background:</span></b><span> Liquid-based cytology (LBC) is a method of manufacturing cyto-diagnostic specimens. Improved accuracy is expected from standardizing specimen production and use of this method is rapidly spreading in oral cytology. On the other hand, LBC reportedly requires training to show peculiar cell findings compared to those of conventional smear cytology (CVC). Few studies have compared detailed cell findings for oral CVC and LBC.</span><span> </span><b><span>Objec</span></b><b><span>tives:</span></b><span> The aim of this study was to compare cytological findings between CVC </span><span>and LBC using cytomorphological image analysis.</span><span> </span><b><span>Materials and Methods:</span></b><span> Cytological specimens were collected from 20 patients (negative for squamous neoplasia in 10, dysplasia in 5, squamous cell carcinoma in 5) and 5 controls of the tongue between January 2017 and December 2018. Two different preparation techniques were investigated cytomorphologically for CVC and LBC (BD Cytorich</span><sup><span style="vertical-align:super;">TM</span></sup><span>).</span><span> </span><b><span>Results:</span></b><span> LBC showed significantly higher cell numbers tha</span><span>n CVC for all lesions. LBC-to-CVC ratio ranged from 9.52 (hyp</span><span>erkeratosis) to 1.87 (deep cells in oral squamous cell carcinoma (OSCC)). Nuclear area of </span><span>normal, hyperkeratosis, and inflammation were significantly higher in LBC tha</span><span>n those of CVC. Hyperchromasia was significantly more frequent with </span><span>CVC than with LBC for hyperkeratosis, inflammation, dysplasia and OSCC. There was no significant difference in circularity between CVC and LBC </span><span>among all lesions. </span><b><span>Conclusion:</span></b><span> Only one cytomorphological disadvantage was </span><span>se</span><span>en with LBC, in the form of decreased hyperchromasia. Further </span><span>clarification of the advantages and disadvantages of LBC is needed, including management of precision and screening practices.
文摘Cancer of the corpus uteri remains the most common gynecological related cancer in developed countries. Cytology, after the induction of liquid based cytology, has reemerged as a possible first line non-interventional diagnostic procedure with promising results. Apart from slide preparation for cytology diagnosis, LBC allows the application of elaborate molecular tests on the residual material. Samples from 74 symptomatic women were collected in ThinPrep?PreservCyt medium, from witch immunocytochemical and molecular tests were performed. Final diagnosis of 39 endometrioid carcinomas, 20 non-endometrioid carcinomas and 15 non-malignant was set after hysterectomy. Topoisomerase IIa expression was common (42%) in both types of cancer. Promoter methylation analysis revealed that hMLH1 is commonly methylated in cancers (52.7%), CDKN2A and MGMT less often (27.1%) and RARB rarely methylated (8.4%). BRAF activating mutation V600E was a rare event (8.4%) only found in low grade endometrioid carcinomas. Topoisomerase IIa expression correlated with BRAF mutations, hMLH1 and to lesser extent with CDKN2A methylation. Almost none of the biomarkers were positive in cytological negative or hyperplastic without atypia samples. Detection of methylation in any gene displayed sensitivity, specificity, PPV and NPV similar to cytology of cancer. However, inclusion of cytology diagnosis of hyperlasias with atypia increased sensitivity and NPV of cytology outperforming methylation of any gene. Further evaluation of the panel of promoter methylation, especially in cytology diagnoses of hyperplasia with or without atypia should be evaluated since initial results are promising. Even though methylation of MGMT and RARB are rare events, some patients could be benefit from specific chemotherapeutics that target either of them or the more frequently expressed topoisomerase IIa.
文摘This study examined cells contained in needles used for the collection of breast fine needle aspirates for the detection of malignant cells trapped in the needles. Remnants of cells contained in 50 needles used for the collection of scanty breast fine needle aspirates were examined by the liquid based cytology technique and compared with the conventional cytological technique of specimens in the corresponding syringes. The breast specimens were collected with clean sterile needles attached to the syringes. Smears were made and stained by the conventional method. The needles were removed from the syringes and a fixative was withdrawn into the syringes and the syringes were recapped with the needles. The fixative containing the specimen was then completely discharged into a centrifuge tube through the needles and treated by the liquid based cytology technique. The study revealed that cells were found trapped in all the needles used for the collection of breast FNA. 6% of them were positive for malignancy, similar to results obtained in the conventional method. Needles used for the collection of breast FNA should be examined before malignancy is completely ruled out particularly in extremely scanty specimens with a clinical suspicion of malignancy.
文摘This review gives a short introduction into the principles of ultrasonic measurement techniques for liquids, using cavity resonators. Guidelines for the resonator design in broad-band ultrasonic stxctroscopy as well as in high-resolution single-frequncy or narrowband applications are presented. Deviations of the field configuration in real cells frtxn that in an ideal resonator are discussed and relations for the mode spectrum of cavity fields are given. Recent resonator measurement procedures and methods of data evaluation are mentioned briefly. Some examples of measurements show the extended usability of ultrasonic resonator techniques in basic science and in a wide range of applications for rrkaterials characterization, in manufacturing processes, as well as in control routines.
文摘Background: The detection of vaginal intraepithelial neoplasia (VAIN) in cervical samples is not a common finding. Therefore, we aimed to report VAINs detected in liquid-based cytology (LBC) from women examined at Hospital das Clínicas of Faculty of Medicine, Sao Paulo State University. Materials and Methods: We evaluated LBC samples from women referred to gynecology examination for different reasons (previous abnormal PapTest, follow up of treated cervical lesion, ecc) and women examined for regular screening proposals, and compared with biopsy diagnoses, including the controversial diagnoses of vaginal intraepithelial neoplasia (VAIN). Results: From 1866 patients, 1423 (76.3%) cases were negative and 443 (23.7%) were positive for any cellular alteration. Age of patients ranged from 12 to 86 years. We detected 25 histologically confirmed VAIN (1.3% p = 0.0002 by Fisher’s exact test IC 95% 0.0090 - 0.0198) and 1.1% VAIN (p = 0.0031 by Fisher’s exact test IC 95% 0.0077 - 0.0179). Conclusion: The identification of VAIN in routine is feasible;the professionals involved with cytological examination should be aware of these lesions in Pap test samples.
文摘Immiscible alloys have attracted growing interest for their valuable physical and mechanical properties. However, their production is difficult because of metallurgical problems in which there is a serious tendency for gravity separation in the region of the miscibility gap. So far the study on the liquid separation mechanism is still one of the important projects in the spatial materials science and the spatial fluid science. The studied results about the liquid phase separating mechanism of immiscible alloys are presented, at the same time the preparation techniques of homogeneous immiscible alloys are summarized, and the existing problems and the related researching areas in the future are also pointed out.
基金Supported by Project of National Natural Science Foundation of Shandong Province(ZR2015PH015)
文摘[Objectives]To study the immediate-release mechanism of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets by liquid-solid compacts technique.[Methods] Taking dehydrotrametenolic acid and dehydroeburicoic acid as indicators,differences in dissolution were compared between liquid-solid compressed tablets and crude drug of total triterpenoids in Poriae Cutis;crude drug,powder of liquid-solid compressed tablets and excipients of liquid-solid compressed tablets were characterized by differential scanning calorimetry( DSC).[Results]Liquid-solid compact technique could significantly improve dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid. The total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in Poriae Cutis total triterpenoids tablets was 92%,t50( time for 50% total dissolution rate) and t_D( time for 63. 2% total dissolution rate) were 11. 18 min and 22. 71 min; the total dissolution rate of dehydrotrametenolic acid and dehydroeburicoic acid in crude drug of total triterpenoids was 29%,and t50 and t_D were231. 06 min and 359. 23 min. DSC showed that there was no mutual interaction between excipients and total triterpenoids in Poriae Cutis; on the DSC curve for powder of liquid-solid compressed tablets,the absorption peak vanished completely,indicating that the drug exists in the amorphous form in the liquid-solid powder.[Conclusions] Liquid-solid compact technique can increase the dissolution rate of total triterpenoids in Poriae Cutis and allow rapid release of poorly water soluble drugs.
文摘AIM To evaluate the cytological diagnostic capacity and sample quality of the slow-pull technique and compare them with different suction techniques.METHODS From July 2010 to December 2015, 102 patients with pancreatic solid lesions who underwent endoscopic ultrasound-guided fine-needle aspiration(EUS-FNA) with 22-gauge needles were retrospectively evaluated. EUS-FNA diagnosis was based on a cytological examination, and final diagnosis was based on a comprehensive standard of cytological diagnosis, surgical pathology and clinical or imaging follow-up. Cytological specimens were characterized for cellularity and blood contamination. The cytological diagnostic capacity and sample quality of the slow-pull technique and suction techniques with 5-m L/10-m L/20-m L syringes were analyzed.RESULTS Of all of the EUS-FNA procedures, the slow-pull technique and suction techniques with 5-m L/10-m L/20-m L syringes were used in 31, 19, 34 and 18 procedures, respectively. There were significant differences between these four suction techniques in terms of cytological diagnostic accuracy(90.3% vs 63.2% vs 58.8% vs 55.6%, P = 0.019), sensitivity(88.2% vs 41.7% vs 40.0% vs 36.4%, P = 0.009) and blood contamination(score ≥ 2 for 29.0% vs 52.6% vs 70.6% vs 72.2%, P = 0.003). The accuracy and sensitivity of the slow-pull technique were significantly higher than those of the suction techniques using 5-m L(P = 0.03, P = 0.014), 10-m L(P = 0.005; P = 0.006) and 20-mL syringes(P = 0.01, P = 0.01). Blood contamination was significantly lower in the slow-pull technique than in the suction techniques with 10-m L(P = 0.001) and 20-mL syringes(P = 0.007).CONCLUSION The slow-pull technique may increase the cytological diagnostic accuracy and sensitivity with slight blood contamination during EUS-FNA when using 22-gauge needles for solid pancreatic masses.
文摘Nanometer powders can be produced by an electrohydrodynamic technique. The breakup mechanism of the metal droplets generated by the electrohydrodynamic technique was analysed. It showed that the applied voltage, the electric field Btrength, the properties of the molten metal and the volumetric flow rate of the molten metal directly effect the breat-up extent of the metal droplets.
文摘目的探索并验证基于华为云ModelArts平台构建的深度学习模型在宫颈液基细胞学(liquid-based cytology,LBC)非典型细胞诊断中的应用价值,并评估其对不同诊断经验医师的辅助效果。方法回顾性分析2020年东莞市人民医院1044例宫颈脱落细胞学标本,采用华为云ModelArts平台开发的人工智能(artifical intelligence,AI)辅助诊断系统与初级、中级、高级医师进行诊断比对,计算灵敏度、特异度、精确率、符合率、曲线下面积(area under the curve,AUC)等指标,评估AI系统的诊断效能及其对不同年资医师的辅助诊断效果。采用McNemar检验比较AI系统与人工诊断的差异。结果在1044例宫颈脱落细胞学标本中,AI系统在非典型细胞检出的灵敏度和特异度分别为98.96%和89.15%,均高于初级医师(81.95%和91.81%)。AI系统的总体诊断精确率为93.68%,显著高于初级医师(87.26%,P<0.001)。AI辅助可显著提高初级医师的诊断性能,灵敏度从80.1%提升至96.5%,特异度从85.6%提升至92.3%。结论本研究构建的AI辅助宫颈细胞学诊断系统性能优越,尤其能显著提高初级医师的诊断水平,具有良好的临床应用前景。