A sensitive and rapid analytical procedure was established to detrmine strychnie alkaloids in diverse biological specimens. Samples were treated with hydrochloric acid to precipitate protein and scanned in thin-layer ...A sensitive and rapid analytical procedure was established to detrmine strychnie alkaloids in diverse biological specimens. Samples were treated with hydrochloric acid to precipitate protein and scanned in thin-layer chromatograpic scanner in zig-zag dual-wavelength reflection mode.Detection sensitivity for strychniue was about 0. 05 μg. Quantification recoveries were between89. 2% and 99. 5% with RSD between 2. 92% and 13. 21 %. The method was applied to investigate thedistribution or strychnine in intoxicated rats. The results showed that there were remarkable difference in organs or tissues with the aigaest concentration 4. 56±1. 41μg/g in liver. Diversification experiment proves the method also could be used to analyze other alkaloids in biological samples if theconditions slightly modified.展开更多
A new method for analysing the ingredients of rhynchophylline and isrhynchophylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thin layer chromatographic（TLC） and the surface-enhanced raman spect...A new method for analysing the ingredients of rhynchophylline and isrhynchophylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thin layer chromatographic（TLC） and the surface-enhanced raman spectroscopic（SERS） technics is reported. The results show that the characteristic spectral bands of rhynchophylline can be obtained from the TLC spot with minimum amount of rhynchophylline sample of about 2 5 mm diameter. The difference between the SERS and solid spectra are found. Spectral band at 1 397 cm-1 was obtained wits great enhanced want. Molecule was absorbed in surface silver sol by pair of electrons in —OCH3. Isrhynchophylline sample spectrum band at 1 615 cm-1 obtained the greatest enhance. Isrhynchophylline molecule was absorbed in surface silver sol by pair of electron at N atom in ring indole. An absorption model of rhynchophylline and isrhynchophylline and silver sol is proposed. That effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.展开更多
文摘A sensitive and rapid analytical procedure was established to detrmine strychnie alkaloids in diverse biological specimens. Samples were treated with hydrochloric acid to precipitate protein and scanned in thin-layer chromatograpic scanner in zig-zag dual-wavelength reflection mode.Detection sensitivity for strychniue was about 0. 05 μg. Quantification recoveries were between89. 2% and 99. 5% with RSD between 2. 92% and 13. 21 %. The method was applied to investigate thedistribution or strychnine in intoxicated rats. The results showed that there were remarkable difference in organs or tissues with the aigaest concentration 4. 56±1. 41μg/g in liver. Diversification experiment proves the method also could be used to analyze other alkaloids in biological samples if theconditions slightly modified.
文摘A new method for analysing the ingredients of rhynchophylline and isrhynchophylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thin layer chromatographic（TLC） and the surface-enhanced raman spectroscopic（SERS） technics is reported. The results show that the characteristic spectral bands of rhynchophylline can be obtained from the TLC spot with minimum amount of rhynchophylline sample of about 2 5 mm diameter. The difference between the SERS and solid spectra are found. Spectral band at 1 397 cm-1 was obtained wits great enhanced want. Molecule was absorbed in surface silver sol by pair of electrons in —OCH3. Isrhynchophylline sample spectrum band at 1 615 cm-1 obtained the greatest enhance. Isrhynchophylline molecule was absorbed in surface silver sol by pair of electron at N atom in ring indole. An absorption model of rhynchophylline and isrhynchophylline and silver sol is proposed. That effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.
