TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu

[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources.

Identification of Tibetan and Qiang Edible and Medicinal Herbs Cardamine tangutorum and Cardamine macrophylla

[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.

ANALYSIS OF HUMAN PLACENTA BY ^(31)P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT

Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.

Pharmacognosy Identification of Xiaohuangcao (Dendrobium loddigesi Rolfe)

[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.

Simultaneous qualitative identification of all medicinal herbs in Zaoren Anshen capsule by thin-layer chromatography

The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TLC),a simple and classic approach for qualitatively characterizing and examining quality markers of natural products,has been widely used in the characterization and quality control of traditional Chinese medicines.Zaoren Anshen(ZRAS)capsule,prepared from three medicinal herbs of fried Ziziphi Spinosae Semen,Salvia Miltiorrhiza Radix et Rhizoma,and vinegar-processed Schisandrae Chinensis Fructus,is a famous PCM in China for the treatment of insomnia,amnesia,and dizziness in clinical practice.However,no effective method is available so far for simultaneous identification and examination of all the three medicinal herbs of ZRAS capsule.In the present study,we developed a TLC method via twice-development and visualization by UV light or chromogenic agent,which could be used for simultaneous qualitative identification of all the three medicinal herbs of ZRAS capsule in one plate.Moreover,the sample preparation method was optimized.The developed TLC method was rapid,simple,low-cost,and effective,and thus it could be used for quality control of ZRAS capsule.

大蒜中低聚糖的TLC鉴别和HPLC-RID含量测定

目的建立大蒜中低聚糖的薄层色谱(TLC)法和高效液相-示差折光检测器(HPLC-RID)法,用于大蒜中低聚糖的鉴别和含量测定。方法采用TLC法大蒜中果糖、蔗糖、蔗果三糖和蔗果四糖进行鉴别,以冰乙酸-氯仿-水(21/18/4)为展开系统,苯胺-二苯胺溶液为显色剂;采用HPLC-RID法测定大蒜中果糖、蔗糖、蔗果三糖和蔗果四糖的含量,色谱柱为碳水化合物分析柱(4.6×250 mm,4μm);流动相:乙腈-水(75/25),柱温:35℃,流速:1.0 mL/min,进样量:15μL。结果薄层色谱中可以检测到果糖、蔗糖、蔗果三糖和蔗果四糖4种低聚糖。果糖、蔗糖、蔗果三糖和蔗果四糖分别在0.03~0.81、0.5~16.04、0.02~0.65、0.02~0.64 mg/mL范围线性关系良好。4种糖类低、中、高浓度平均回收率(n=3)分别为98.0%、99.7%、100.3%和98.2%;测定新疆5种不同海拔(1733、1680、1250、2800、2300 m)大蒜中果糖、蔗糖、蔗果三糖、蔗果四糖的含量分别为0.34~0.54、6.54~8.74、0.45~0.61、0.04~0.15 mg/g。结论本研究建立的大蒜中低聚糖的定性、定量分析方法准确可靠、重现性好,可用于大蒜中低聚糖的鉴别和含量测定。

黎药弯柄灵芝质量标准初步研究

目的:初步建立黎药弯柄灵芝的质量标准。方法:对弯柄灵芝药材的性状进行鉴定,对其显微特征进行鉴别;参照《中国药典》2020年版方法,采用薄层色谱法(TLC)对药材进行定性鉴别,采用分光光度法测定药材中的多糖和三萜及甾醇含量。结果:明确了弯柄灵芝的性状及显微特征,建立了专属的TLC定性鉴别法;初步规定了弯柄灵芝多糖含量不得低于0.80%,三萜及甾醇含量不得低于0.90%。结论:本研究建立的弯柄灵芝药材质量标准可为其质量控制及资源可持续利用提供依据。

红麻花甲醇可溶性色素TLC分离与成分鉴定

以红麻花为原料,通过对红麻花甲醇可溶性色素的薄板层析(TLC)分离研究,得到其TLC最佳展层剂是:V(乙酸乙酯)∶V(水)∶V(甲酸)∶V(乙酸)=70∶30∶3∶2。以最佳展层剂展开花中的色素,得到4条颜色深浅不一的色带,反映该花色素至少含4种色素单体成分。对色素母液及第一单体成分样液进行290～700 nm扫描测定表明,色素母液在253.9 nm及372.6 nm各有一特征吸收峰;色素第一单体样液的特征吸收峰在252.7 nm及369.0 nm。初步鉴定结果揭示:红麻花甲醇可溶性色素成分主要为黄酮醇类。

医疗机构制剂芪风通窍合剂的系统质量提升研究

目的建立定性鉴别和含量测定方法,为芪风通窍合剂质量标准提升提供参考依据。方法采用薄层色谱法对处方中黄芪、辛夷和广藿香进行定性鉴别;以黄芪甲苷为对照品,通过高效液相色谱法对处方中的主要药味黄芪进行含量测定。结果在采用的薄层色谱条件下,黄芪、辛夷和广藿香色谱斑点清楚,分离效果好,无干扰;在高效液相色谱含量测定中,黄芪甲苷线性方程为Y=1.4118X+4.8326,相关系数R 2=0.9984,表明黄芪甲苷在0.39~3.90μg的进样量范围内具有良好的线性关系,该方法平均回收率为99.78%,RSD值为1.02%(n=9)。结论建立的定性鉴别和含量测定方法比较简便、准确、灵敏、重现性好,可用于芪风通窍合剂质量控制。

Separation of liquefied product of Salix psammophila by column chromatography and structure analysis of its components

The liquefied product of Salixpsammophila wood was separated by thin-layer chromatography （TLC） and column chromatography, and its structure was identified by nuclear magnetic resonance （NMR） spectra in our study. The separation result indicates that the sample of liquefied S. psammophila contained at least two categories of components. The structure of the main components was guaiacyl C-1, C-2 of the hydroxyphenyl propane, i.e., the aromatic nucleus protons of lignin. Degradation and polycondensation reactions occurred when the S. psammophila wood was liquefied in phenol. Polycondensation reactions occurred among the depolymerization products from cellulose, the aromatic depolymerization products from lignin and the products of the displacement reactions between phenoxide ion and cellulose.

医疗机构制剂肩凝灵胶囊的质量标准提升研究

目的 建立定性鉴别和含量测定方法,为肩凝灵胶囊质量标准提升提供参考依据。方法 采用薄层色谱法对处方中延胡索、葛根、赤芍进行定性鉴别;以葛根素为对照品,通过高效液相色谱法对处方中的主要药味葛根进行含量测定。结果 在采用的薄层色谱条件下,延胡索、葛根、赤芍色谱斑点清楚,分离效果好,无干扰;在高效液相色谱含量测定中,葛根素线性方程为Y=0.808 5X-0.170 97,相关系数R^(2)=0.999 89,表明葛根素在1.901 3~28.519 8μg/ml范围内具有良好的线性关系,该方法平均回收率为99.8%,RSD值为1.4%(n=9)。结论 建立的定性鉴别和含量测定方法比较简便、准确、灵敏、重现性好,可用于肩凝灵胶囊质量控制。

杜仲籽油中α-亚麻酸的甲酯化方法优化

以超临界CO2萃取的杜仲籽油为原料,对其主要成分α-亚麻酸的甲酯化方法进行筛选和优化。在确定合适的α-亚麻酸甲酯化方法基础上,考察了催化剂用量、催化剂浓度、反应温度、反应时间等对α-亚麻酸甲酯化效果的影响,确定最佳的甲酯化条件为:用2 mL石油醚-苯(V(石油醚)∶V(苯)=1∶1)为溶剂将0.2 g杜仲籽油溶解,加入5 mL0.2 mol/L的KOH-甲醇溶液作催化剂,反应温度50℃,反应时间30 min。薄层展开检识显示在该条件下,杜仲籽油中α-亚麻酸甲酯化反应完全,气相色谱检测表明甲酯化率可达92.59%。

蒙药小叶锦鸡儿的生药鉴定

目的对蒙药小叶锦鸡儿进行系统的生药学鉴定,为其鉴别及开发利用提供科学依据。方法采用原植物、性状、显微、薄层鉴定方法。结果通过原植物、性状、显微、薄层色谱图研究能够很好地鉴定药材。结论方法简便易操作,可作为蒙药小叶锦鸡儿的定性鉴别方法。

民族药白花丹和红花丹的药材比较研究

目的:对民族药白花丹和红花丹进行系统的药材比较研究,为其鉴别提供科学依据.方法:采用原植物、性状、显微、薄层鉴定方法.结果:通过原植物、性状鉴别、显微鉴别、薄层色谱鉴别能够很好地鉴定两种原植物.结论:原植物、性状鉴别、显微鉴别方法简便易操作,可作为两种民族药材定性鉴别.

民族药鬼针草的生药学鉴定

采用原植物、性状、显微、薄层鉴别方法对民族药鬼针草进行系统的生药学鉴定,为其鉴别及应用提供科学依据.结果表明:通过原植物、性状鉴别、显微鉴别、薄层色谱鉴别能够很好地鉴定该药材.

维吾尔药材睡莲花的生药鉴定

目的 :为用药准确和进一步开发利用植物资源 ,制订药材标准提供依据。方法 :应用性状、显微、薄层及其紫外鉴别方法。结果 :详细描述了该生药的性状及显微特征 ;薄层板上斑点明显 ;紫外吸收光谱在 2 71.2 0 nm处有明显吸收。结论

藏药甘松及翼首草生药学鉴定

采用原植物、性状、显微、薄层鉴定的方法对藏药甘松、翼首草进行系统的生药学鉴定,为其鉴别及应用提供科学依据。结果表明:通过原植物、性状、显微、薄层色谱图研究能够很好地鉴定原植物。

藏药白花刺参的生药学鉴定

目的对藏药白花刺参进行系统的生药学鉴定,为其鉴别及应用提供科学依据。方法采用原植物、性状、显微、薄层鉴定方法。结果通过原植物、性状、显微、薄层色谱图研究能够很好的鉴定原植物。结论该方法简便易操作,可作为藏药白花刺参定性鉴别。

民族药马兰的生药学鉴定

目的对民族药马兰进行系统的生药学鉴定,为其鉴别提供科学依据。方法采用原植物、性状、显微、薄层鉴定方法。结果通过原植物、性状鉴别、显微鉴别、薄层色谱鉴别能够很好地鉴定原植物。结论这些方法简便易操作,可作为该民族药材定性鉴别方法。

民族药透骨草的生药学研究

目的对多民族常用的植物药透骨草进行生药学鉴定,为其鉴别及应用提供科学依据。方法采用原植物、性状、显微、薄层鉴别方法。结果透骨草在原植物、性状、显微、薄层色谱等方面具有专属性的特征。结论通过原植物、性状、显微、薄层色谱图能够很好地鉴定透骨草。