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Finite element simulation of welded thin-walled stainless steel container based on SYSWELD
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作者 何晓祥 俞建荣 +2 位作者 郭云龙 杨德宇 王岚 《China Welding》 EI CAS 2012年第2期23-27,共5页
The temperature field and stress fields of 18 - 8 stainless steel container structure were computed during and after tungsten inert gas (TIG) arc welding based on the SYSWELD software. The convection, radiation and ... The temperature field and stress fields of 18 - 8 stainless steel container structure were computed during and after tungsten inert gas (TIG) arc welding based on the SYSWELD software. The convection, radiation and conduction were all considered during the simulation process as well as temperature-dependent material properties. The results show that the peak temperature occurs on the heat source location. Steep temperature gradients are observed ahead of the heat source. Axial tensile stress and hoop compressive stress are observed in the weld seam between cylinder and head. Axial compressive stress and hoop tensile stress are observed near the weld seam between cylinder and heads. Axial compressive stress and hoop tensile stress are observed in the axial weld seam of cylinder. Axial tensile stress and hoop compressive stress are observed near the axial weld seam of cylinder. The aim of the above research is to provide a basic theory and some calculation methods for the thin-walled container welding technology so that the failures of these structures in service due to residual stresses may be minimized. 展开更多
关键词 numerical simulation thin-walled containers finite element simulation temperature field stress field
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Closed-form solution to thin-walled box girders considering effects of shear deformation and shear lag 被引量:18
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作者 周旺保 蒋丽忠 +1 位作者 刘志杰 刘小洁 《Journal of Central South University》 SCIE EI CAS 2012年第9期2650-2655,共6页
Considering three longitudinal displacement functions and uniform axial displacement functions for shear lag effect and uniform axial deformation of thin-walled box girder with varying depths,a simple and efficient me... Considering three longitudinal displacement functions and uniform axial displacement functions for shear lag effect and uniform axial deformation of thin-walled box girder with varying depths,a simple and efficient method with high precision to analyze the shear lag effect of thin-walled box girders was proposed.The governing differential equations and boundary conditions of the box girder under lateral loading were derived based on the energy-variational method,and closed-form solutions to stress and deflection corresponding to lateral loading were obtained.Analysis and calculations were carried out with respect to a trapezoidal box girder under concentrated loading or uniform loading and a rectangular box girder under concentrated loading.The analytical results were compared with numerical solutions derived according to the high order finite strip element method and the experimental results.The investigation shows that the closed-form solution is in good agreement with the numerical solutions derived according to the high order finite strip method and the experimental results,and has good stability.Because of the shear lag effect,the stress in cross-section centroid is no longer zero,thus it is not reasonable enough to assume that the strain in cross-section centroid is zero without considering uniform axial deformation. 展开更多
关键词 shear lag effect thin-walled box girder energy-variational method shear deformation closed-form solution
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Improved concept models for straight thin-walled columns with box cross section 被引量:4
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作者 Yu-cheng LIU 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2008年第11期1473-1479,共7页
This paper focuses on developing improved concept models for straight thin-walled box sectional columns which can better predict the peak crushing force that occurs during crashworthiness analyses. We develop a nonlin... This paper focuses on developing improved concept models for straight thin-walled box sectional columns which can better predict the peak crushing force that occurs during crashworthiness analyses. We develop a nonlinear translational spring based on previous research and apply such a spring element to build the enhanced concept models. The work presented in this article is developed on the basis of the publication of the author (Liu and Day, 2006b) and has been applied in a crashworthiness design issue, which is presented by the author in another paper (Liu, 2008). 展开更多
关键词 Concept model Peak crushing force box section Nonlinear translational springs Crashworthiness analysis thin-walled column
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Lateral static and dynamic characteristics of thin-walled box girder 被引量:1
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作者 甘亚南 周广春 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第1期101-106,共6页
To analyze the static and dynamic behaviors of the thin-walled box girder in its lateral webs in consideration of shear lag effect and shear deformation, an approach based on the minimum potential principle is introdu... To analyze the static and dynamic behaviors of the thin-walled box girder in its lateral webs in consideration of shear lag effect and shear deformation, an approach based on the minimum potential principle is introduced in this paper. Both static and dynamic response equations as well as the corresponding natural boundary conditions of the box girder are deduced. Meanwhile, three generalized displacement functions: w (x) , U(x) and O(x) are employed and their differences in the calculus of variation are quantitatively investigated. The comparison of finite shell element results with analytical results of calculation examples validates the feasibility of the proposed approach. 展开更多
关键词 thin-walled box girder shear lag effect static and dynamic characteristics energy-variation principle
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A comparative study on the hull strength of a ship with thin-walled box girders
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作者 NIE Wu MA Chun-yan 《Journal of Marine Science and Application》 2006年第3期11-16,共6页
Based on the Finite Element Analysis and Thin Walled-Box girder Mechanics, two design concepts of adding box girders under main deck in order to increase the hull strength of ship are presented. By comparison and anal... Based on the Finite Element Analysis and Thin Walled-Box girder Mechanics, two design concepts of adding box girders under main deck in order to increase the hull strength of ship are presented. By comparison and analysis on the longitudinal strength, torsion strength and deck buckling between designed concepts and the original concept, it is found that by adding box girders under the main deck, the weight of hull structure is increased by lower than 10%, but the stress on the plate of the main deck is reduced by about 10%, the stress on the plating of the second deck is reduced about 20%. The shear stress on the plating of both of the main deck and second deck is reduced, but the shear stresses in several nodes are increased. Also the capability of resisting damage to ship is obviously increased by adding box girders under the main deck. The deck buckling is also increased by more than 90%. Consequently, the box girders added under the main deck are useful and effective to increase the strength of hull and ship survivability. 展开更多
关键词 thin-walled box girder longitudinal strength torsion strength deck buckling ship survivability
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Powell's optimal identification of material constants of thin-walled box girders based on Fibonacci series search method
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作者 张剑 叶见曙 周储伟 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2011年第1期97-106,共10页
A dynamic Bayesian error function of material constants of the structure is developed for thin-walled curve box girders. Combined with the automatic search scheme with an optimal step length for the one-dimensional Fi... A dynamic Bayesian error function of material constants of the structure is developed for thin-walled curve box girders. Combined with the automatic search scheme with an optimal step length for the one-dimensional Fibonacci series, Powell's optimization theory is used to perform the stochastic identification of material constants of the thin-walled curve box. Then, the steps in the parameter identification are presented. Powell's identification procedure for material constants of the thin-walled curve box is compiled, in which the mechanical analysis of the thin-walled curve box is completed based on the finite curve strip element (FCSE) method. Some classical examples show that Powell's identification is numerically stable and convergent, indicating that the present method and the compiled procedure are correct and reliable. During the parameter iterative processes, Powell's theory is irrelevant with the calculation of the FCSE partial differentiation, which proves the high computation efficiency of the studied methods. The stochastic performances of the system parameters and responses axe simultaneously considered in the dynamic Bayesian error function. The one-dimensional optimization problem of the optimal step length is solved by adopting the Fibonacci series search method without the need of determining the region, in which the optimized step length lies. 展开更多
关键词 Powell's theory thin-walled curve box material constant Fibonacci seriessearch method finite curve strip element theory
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THE APPROXIMATE ANALYTICAL SOLUTION FOR THE BUCKLING LOADS OF A THIN-WALLED BOX COLUMN WITH VARIABLE CROSS-SECTION
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作者 谢用九 宁钦海 陈明伦 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 1998年第5期445-456,共12页
For a thin-walled box column with variable cross-section, the three governing equations for torsional-flexural buckling are ordinary differential equations of the second or fourth order with variable coefficients, so ... For a thin-walled box column with variable cross-section, the three governing equations for torsional-flexural buckling are ordinary differential equations of the second or fourth order with variable coefficients, so it is very difficult to solve them by means of an analytic method. In this paper, polynomials are used to approximate the geometric properties of cross-section and certain coefficients of the differential equations. Based on the energy principle and the Galerkin's method, the approximate formulas for calculating the flexural and torsional buckling loads of this kind of columns are developed respectively, and numerical examples are used to verify the correctness of the solutions obtained. The results calculated in this paper provide the basis for demonstrating the stability of thin-walled box columns with variable cross-section. This paper is of practical value. 展开更多
关键词 thin-walled box column with variable cross-section torsional-flexural buckling approximate solutions for buckling loads
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Sex-determining region Y box-containing genes: regulators and biomarkers in gynecological cancers 被引量:4
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作者 Jiali Hu Ke Li +4 位作者 Zhanghuan Li Chao Gao Fei Guo Yingmei Wang Fengxia Xue 《Cancer Biology & Medicine》 SCIE CAS CSCD 2019年第3期462-474,共13页
Sex-determining region Y box-containing genes are transcription factors with roles in multiple biological processes, including cell differentiation, proliferation, and apoptosis.Sex-determining region Y box-containing... Sex-determining region Y box-containing genes are transcription factors with roles in multiple biological processes, including cell differentiation, proliferation, and apoptosis.Sex-determining region Y box-containing genes have also been shown to act as regulators and biomarkers in the progression of many different cancers, including gynecological cancers such as ovarian, cervical,and endometrial cancer.In this review, we summarize the contrasting regulatory roles of Sex-determining region Y box-containing genes in different gynecological cancers, as promotors with high expression levels or as suppressors with low expression levels.Expression levels of Sex-determining region Y box-containing genes were also identified as biomarkers of clinical features, including International Federation of Gynecology and Obstetrics stage, histopathologic grade together with disease-free survival, and treatment efficacy in patients with gynecological cancers.An understanding of the mechanisms whereby Sex-determining region Y box-containing genes regulate the progression of gynecological cancers will aid in the development of novel diagnostic and therapeutic strategies, while analysis of Sex-determining region Y box-containing expression levels will help to predict the prognosis of patients with gynecological cancers. 展开更多
关键词 Sex-determining region Y box-containing gene GYNECOLOGICAL cancer regulator biomarker clinical feature progression
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维生素D_(3)对小鼠支气管哮喘气道炎症和氧化应激反应的作用及其分子机制 被引量:1
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作者 贾斌 梁思敏 《安徽医科大学学报》 CAS 北大核心 2024年第1期58-63,共6页
目的探究维生素D_(3)(VitD_(3))在小鼠支气管哮喘气道炎症和氧化应激反应中的作用和相关分子机制。方法将28只雌性C57BL/6小鼠随机分为对照组(Ctrl)和模型组。模型组小鼠采用卵清蛋白(OVA)致敏法建立哮喘模型后,将其分为哮喘(Asthma)组... 目的探究维生素D_(3)(VitD_(3))在小鼠支气管哮喘气道炎症和氧化应激反应中的作用和相关分子机制。方法将28只雌性C57BL/6小鼠随机分为对照组(Ctrl)和模型组。模型组小鼠采用卵清蛋白(OVA)致敏法建立哮喘模型后,将其分为哮喘(Asthma)组、VitD_(3)处理(Asthma+VitD_(3))组和叉头盒O1(FOXO1)抑制剂AS1842856处理(Asthma+AS)组。测定各组小鼠肺阻力(LR)变化。采用ELISA法检测肺泡灌洗液(BALF)中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-18的含量。Western blot检测肺组织中FOXO1和NOD样受体热蛋白结构域相关蛋白3(NLRP3)、半胱氨酸天冬氨酸酶-1(Caspase-1)和凋亡斑点蛋白(ASC)的表达水平。结果与Ctrl组相比,Asthma组小鼠的LR升高(P<0.01)。与Asthma组相比,Asthma+VitD_(3)组和Asthma+AS组小鼠的LR降低(P<0.05),Asthma+VitD_(3)组与Asthma+AS组小鼠的LR变化差异无统计学意义。与Ctrl组相比,Asthma组、Asthma+VitD_(3)组和Asthma+AS组小鼠BALF中TNF-α、IL-1β与IL-18含量均增加(P<0.01),肺组织中NLRP3、Caspase-1和ASC蛋白表达水平均升高(P<0.01);与Asthma组相比,Asthma+VitD_(3)组和Asthma+AS组小鼠BALF中上述炎症因子含量均减少(P<0.05),肺组织中NLRP3、FOXO1、Caspase-1和ASC蛋白表达均降低(P<0.05);与Asthma+VitD_(3)组相比,Asthma+AS组中除FOXO1蛋白表达水平升高外(P<0.05),上述其他检测指标差异均无统计学意义。结论VitD_(3)可减轻OVA诱导的小鼠哮喘症状,改善气道炎症程度和降低氧化应激水平,且其机制可能与FOXO1/NLRP3轴的下调有关。 展开更多
关键词 维生素D_(3) 哮喘 叉头盒O1 NOD样受体家族蛋白3炎症小体
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AR、SKP2、SOX10、PD-L1及TIL表达在三阴性乳腺癌中的意义
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作者 刘娟 殷丽娟 范德生 《诊断学理论与实践》 2024年第2期162-172,共11页
目的:探索雄激素受体(androgen receptor,AR)、S期激酶相关蛋白2(S-phase kinase-associated protein 2,SKP2)、性别决定区Y相关的HMG盒含因子10(sry-related HMG box-containing factor 10,SOX10)、程序性死亡配体1(programmed death-l... 目的:探索雄激素受体(androgen receptor,AR)、S期激酶相关蛋白2(S-phase kinase-associated protein 2,SKP2)、性别决定区Y相关的HMG盒含因子10(sry-related HMG box-containing factor 10,SOX10)、程序性死亡配体1(programmed death-ligand 1,PD-L1)及肿瘤浸润性淋巴细胞(tumor infiltrating lymphocyte,TIL)在三阴性乳腺癌(triple negative breast cancer,TNBC)表达与临床病理特征和预后的关系。方法:根据苏木精-伊红染色(hematoxylineosin, HE)染色切片评判109例TNBC瘤巢内TIL的比例,采用Leica Bond-Max全自动免疫组化仪检测TNBC组织中AR、SKP2、SOX10、PD-L1的表达。分析以上各生物指标与临床病理特征间的关系,并采用kaplan-Meier、Log-rank进行生存分析。结果:95例患者获得随访,中位随访时间为48个月,中位无病生存时间(disease-free survival, DFS)为42个月,中位总生存时间(overall survival, OS)48个月。在TNBC中,AR阳性表达与淋巴结转移阴性(P=0.009)、肿瘤最大径<2 cm(P=0.008)相关,TIL高表达与低级别TNBC相关(P=0.007),SKP2阳性表达与神经/脉管侵犯阳性(P=0.011)、高级别TNBC相关(P=0.002),SOX10阳性表达与淋巴结转移阳性(P=0.022)、高级别TNBC(P=0.005)相关,PD-L1阳性表达与淋巴结转移阳性(P=0.020)、神经/脉管侵犯阳性(P=0.006)、高级别TNBC(P=0.042)相关。生存分析显示,SKP2、SOX10阳性表达与更差的DFS(P=0.007、P<0.001)和OS(P=0.013、P<0.001)相关,TIL高表达与更好的DFS(P=0.016)及OS(P=0.004)相关。在生物表志物的联合表达中,AR+/SKP2-、AR+/SOX10-与更好的DFS(P=0.004、P<0.001)及OS(P=0.007、P=0.001)相关,SOX10+/低TIL、PD-L1+/低TIL与更差的DFS(P<0.001、P=0.008)及OS(P=0.001、P=0.002)相关,AR-/低TIL者具有更差的OS(P=0.014)。SKP2(HR=4.143,95%CI为1.578~10.875)、SOX10(HR=7.578,95%CI为2.067~27.782)的阳性表达是影响TNBC患者DFS的独立预后因子,SKP2(HR=3.758,95%CI为1.400~10.084)、SOX10(HR=5.131,95%CI为1.316~20.000)及TIL(HR=0.375,95%CI为0.154~0.917)的阳性表达是TNBC患者OS的独立预后因子(P均<0.05)。结论:在TNBC中,AR阳性、TIL高表达与具有更好预后的临床病理特征相关,SKP2、SOX10和PD-L1与具侵袭性的临床病理特征相关。SKP2、SOX10及TIL表达与TNBC预后相关,提示这些生物指标可能成为TNBC新的预后因子,同时它们也有可能成为潜在的治疗靶点。 展开更多
关键词 三阴性乳腺癌 雄激素受体 S期激酶相关蛋白2 性别决定区Y相关的HMG盒含因子10 程序性死亡配体1 肿瘤浸润性淋巴细胞
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锚蛋白重复序列和细胞因子信号传导抑制因子盒蛋白13通过锌指家族核转录因子2/溶质载体家族7成员11介导的铁死亡促进动脉粥样硬化的机制研究 被引量:1
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作者 钟妮尔 王菲 +1 位作者 邓纪钊 杨光 《临床内科杂志》 CAS 2024年第6期415-419,共5页
目的探讨锚蛋白重复序列和细胞因子信号传导抑制因子盒蛋白13(ASB13)在动脉粥样硬化(AS)进展中的作用和分子机制。方法将载脂蛋白E基因敲除(ApoE-/-)小鼠随机分为对照组、AS组、sh-NC组、sh-ASB13组和sh-ASB13+sh-锌指家族核转录因子2(S... 目的探讨锚蛋白重复序列和细胞因子信号传导抑制因子盒蛋白13(ASB13)在动脉粥样硬化(AS)进展中的作用和分子机制。方法将载脂蛋白E基因敲除(ApoE-/-)小鼠随机分为对照组、AS组、sh-NC组、sh-ASB13组和sh-ASB13+sh-锌指家族核转录因子2(SNAI2)组。对照组小鼠给予标准饮食,其余4组均给予高脂饮食,饲喂8周。细胞实验1将HUVECs分为细胞对照组、ox-LDL组、ox-LDL+si-NC组、ox-LDL+si-ASB13组和ox-LDL+si-ASB13+Erastin组。细胞实验2将HUVECs分为ox-LDL+si-NC组、ox-LDL+si-SNAI2组和ox-LDL+si-ASB13+si-SNAI2组。采用HE染色评估组织病理学变化;采用CCK-8分析细胞活力;采用Western blot检测ASB13、SNAI2、溶质载体家族7成员11(SLC7A11)、GPX4蛋白表达水平;采用生化检测试剂盒检测脂质代谢物和Fe2+水平。结果动物实验结果表明,与对照组比较,AS组小鼠血清HDL-C水平及主动脉组织中SLC7A11和GPX4蛋白表达水平均降低,LDL-C、TC、TG、Glu和Fe2+水平及主动脉组织中ASB13蛋白表达水平均升高(P<0.05)。与sh-NC组比较,ASB13敲低抑制AS小鼠的动脉粥样硬化斑块形成,SNAI2敲低则作用相反。细胞实验结果表明,与细胞对照组比较,ox-LDL处理降低HUVECs细胞活力,升高ASB13蛋白表达水平,促进脂质积累和铁死亡;沉默ASB13升高细胞活力,减少脂质积累和铁死亡(P<0.05)。ASB13泛素化降低SNAI2蛋白表达水平,SNAI2与SLC7A11启动子结合促进其转录激活,上调SLC7A11蛋白表达水平。与ox-LDL+si-NC组比较,沉默SNAI2或铁死亡诱导剂Erastin处理逆转了ASB13沉默对HUVECs的保护作用(P<0.05)。结论ASB13可能通过SNAI2/SLC7A11介导的铁死亡促进AS发生与发展。 展开更多
关键词 动脉粥样硬化 锚蛋白重复序列和细胞因子信号传导抑制因子盒蛋白13 锌指家族核转录因子2 泛素化 铁死亡
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波纹夹层结构增强的新型罐式集装箱及其设计方法
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作者 鲍硕 李明轩 +1 位作者 陆晓峰 朱晓磊 《石油化工设备》 CAS 2024年第2期50-58,共9页
罐式集装箱因安全性高、承载量大、性价比高、运输灵活以及可以实现多种运输方式快速切换等特点,成为液体货物点对点运输的主要装备。近年来,大容量、高承载能力成为罐式集装箱发展的主流方向。提出了一种波纹夹层结构增强的新型矩形罐... 罐式集装箱因安全性高、承载量大、性价比高、运输灵活以及可以实现多种运输方式快速切换等特点,成为液体货物点对点运输的主要装备。近年来,大容量、高承载能力成为罐式集装箱发展的主流方向。提出了一种波纹夹层结构增强的新型矩形罐式集装箱,并建立了新型罐式集装箱罐体结构的理论设计方法。应用该方法设计了承载能力为1 MPa的新型罐式集装箱罐体,并将设计结果与有限元仿真计算结果进行对比。研究结果表明,相较于传统罐式集装箱,新型罐式集装箱的容量效率提升了17.2%;理论设计方法计算的应力分布特征与有限元计算结果一致,最大误差为6.21%,该理论设计方法可以满足工程需要。理论设计方法可以对任意正交各向异性夹层结构增强的新型罐式集装箱罐体进行设计,具有较高的可移植性。 展开更多
关键词 罐式集装箱 波纹夹层结构 矩形 罐体 设计
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肝细胞癌患者癌组织中CRIP1、STUB1表达及临床预后意义
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作者 张景 沈晨 +1 位作者 马鹏飞 郑幼伟 《国际检验医学杂志》 CAS 2024年第3期266-271,共6页
目的探讨肝细胞癌患者癌组织中富含半胱氨酸肠蛋白1(CRIP1)、STIP1同源性和包含U-box蛋白1(STUB1)表达及临床预后意义。方法选取2018年2月至2020年2月该院诊治的112例肝细胞癌患者作为研究对象。免疫组化法检测肝细胞癌患者癌组织和癌... 目的探讨肝细胞癌患者癌组织中富含半胱氨酸肠蛋白1(CRIP1)、STIP1同源性和包含U-box蛋白1(STUB1)表达及临床预后意义。方法选取2018年2月至2020年2月该院诊治的112例肝细胞癌患者作为研究对象。免疫组化法检测肝细胞癌患者癌组织和癌旁组织中CRIP1、STUB1表达。分析肝细胞癌患者癌组织CRIP1、STUB1表达与其临床病理特征的关系。Kaplan-Meier生存分析癌组织CRIP1、STUB1表达对肝细胞癌患者预后的影响。COX回归分析影响肝细胞癌预后的因素。结果肝细胞癌患者癌组织中CRIP1阳性率为62.50%(70/112),明显高于癌旁组织[7.14%(8/112)],差异有统计学意义(χ^(2)=76.652,P<0.05)。肝细胞癌患者癌组织中STUB1阳性率为26.23%(32/112),明显低于癌旁组织[82.14%(92/112)],差异有统计学意义(χ^(2)=73.284,P<0.05)。癌组织中CRIP1与STUB1表达呈负相关(r=-0.678,P<0.001)。不同肿瘤TNM分期、组织学分级及肿瘤最大径的肝细胞癌患者癌组织中CRIP1、STUB1阳性率比较,差异有统计学意义(P<0.05)。CRIP1阳性组3年累积生存率明显低于CRIP1阴性组,差异有统计学意义(Log-rankχ^(2)=29.601,P<0.001)。STUB1阴性组3年累积生存率明显低于STUB1阳性组,差异有统计学意义(Log-rankχ^(2)=13.590,P<0.001)。肿瘤TNM分期Ⅱ~Ⅲ期、组织学分级Ⅲ级、肿瘤最大径>5 cm、CRIP1阳性、STUB1阴性是影响肝细胞癌患者预后的独立危险因素。结论肝细胞癌患者癌组织中CRIP1表达上调,STUB1表达下调,临床上可根据肝细胞癌患者癌组织中CRIP1、STUB1表达情况对患者预后进行评估。 展开更多
关键词 肝细胞癌 富含半胱氨酸肠蛋白1 STIP1同源性和包含U-box蛋白1 临床病理特征 预后
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岸边集装箱起重机小型箱体梁翻身吊装工艺
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作者 李俊 《港口装卸》 2024年第5期57-59,共3页
岸边集装箱起重机钢结构由众多箱体梁组合而成,其中前后大梁的左、右梁连接一般通过5根小型联系横梁进行刚性连接。在对连接前后大梁的小型箱体梁翻身吊装方案进行论证的基础上,提出一种翻身移位吊装方案,给出了吊耳翻身吊装和小型箱体... 岸边集装箱起重机钢结构由众多箱体梁组合而成,其中前后大梁的左、右梁连接一般通过5根小型联系横梁进行刚性连接。在对连接前后大梁的小型箱体梁翻身吊装方案进行论证的基础上,提出一种翻身移位吊装方案,给出了吊耳翻身吊装和小型箱体梁附属件吊装工艺要点。该方案可提高小型箱体梁翻身移位及附属件吊装的效率和安全性。 展开更多
关键词 岸边集装箱起重机 箱体梁 翻身 吊装
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FBXW7/MCM7信号轴介导肝癌细胞增殖的研究
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作者 李英 王兴 +3 位作者 李永宁 刘鹏 刘松柏 潘耀振 《贵州医科大学学报》 CAS 2024年第2期191-197,共7页
目的 探讨FBXW7靶向调控MCM7表达对肝癌细胞增殖的影响。方法 采用免疫共沉淀法(CoIP)和免疫荧光染色法(IF),分析肝癌细胞系MHCC-97H中FBXW7与MCM7的互作关系;通过慢病毒感染过表达FBXW7和(或) MCM7,小干扰RNA技术下调FBXW7和(或) MCM7... 目的 探讨FBXW7靶向调控MCM7表达对肝癌细胞增殖的影响。方法 采用免疫共沉淀法(CoIP)和免疫荧光染色法(IF),分析肝癌细胞系MHCC-97H中FBXW7与MCM7的互作关系;通过慢病毒感染过表达FBXW7和(或) MCM7,小干扰RNA技术下调FBXW7和(或) MCM7的表达;采用Western blot检测FBXW7对MCM7蛋白表达的影响,CCK-8实验检测肝癌细胞的增殖活性,EdU细胞增殖检测分析肝癌细胞的增殖能力,平板克隆实验分析肝癌细胞的集落形成能力。结果 在MHCC-97H细胞中,FBXW7与MCM7存在互作关系:过表达FBXW7抑制了MCM7的蛋白表达水平、并抑制了肝癌MHCC-97H细胞的增殖(P <0.05),在过表达FBXW7的基础上增加MCM7的表达后,细胞增殖能力提高(P <0.05);下调FBXW7后细胞中MCM7表达增加、细胞增殖能力增强(P <0.05),而下调FBXW7的同时干扰MCM7表达后,细胞增殖能力减弱(P <0.05)。结论 FBXW7能抑制肝癌细胞增殖,其机制与负向调节肝癌细胞中促癌蛋白MCM7的表达水平有关。 展开更多
关键词 肝癌 F-box和WD重复结构域蛋白7 微小染色体维持蛋白-7 细胞增殖 MHCC-97H细胞
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一种干散货装船敞口自卸箱设计
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作者 张楠 《港口装卸》 2024年第1期36-37,共2页
针对散杂货码头使用自卸车、门机、抓斗配合作业的工艺在装船过程中出现的物料洒落较多、环境污染较大等问题,设计一种全新的干散货装船敞口自卸箱,给出了敞口自卸箱设计要点、敞口自卸箱使用辅助工属具设计和敞口自卸箱作业过程。应用... 针对散杂货码头使用自卸车、门机、抓斗配合作业的工艺在装船过程中出现的物料洒落较多、环境污染较大等问题,设计一种全新的干散货装船敞口自卸箱,给出了敞口自卸箱设计要点、敞口自卸箱使用辅助工属具设计和敞口自卸箱作业过程。应用表明,该敞口自卸箱在保证作业效率的前提下,可有效减少作业过程中的物料洒落。 展开更多
关键词 散杂货 集装斗 敞口自卸箱
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食管鳞癌组织中TIM-3、FOXA2表达与其临床病理特征及预后的关系研究
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作者 张楠 程多 +4 位作者 王晶晶 胡杨 岳文莉 白慧丽 梁芳 《实用癌症杂志》 2024年第3期365-369,共5页
目的探讨食管鳞癌组织中T细胞免疫球蛋白黏蛋白分子3(TIM-3)、叉头框蛋白A2(FOXA2)表达与其临床病理特征及预后的关系。方法选取87例食管鳞癌患者(食管鳞癌组)、50例慢性食管炎患者(对照组)为研究对象。分别比较食管鳞癌组与对照组、不... 目的探讨食管鳞癌组织中T细胞免疫球蛋白黏蛋白分子3(TIM-3)、叉头框蛋白A2(FOXA2)表达与其临床病理特征及预后的关系。方法选取87例食管鳞癌患者(食管鳞癌组)、50例慢性食管炎患者(对照组)为研究对象。分别比较食管鳞癌组与对照组、不同临床病理特征食管鳞癌患者及不同预后食管鳞癌患者TIM-3、FOXA2表达;采用Kaplan-Meier生存曲线进行生存分析,应用Cox比例风险回归模型分析TIM-3、FOXA2表达与食管鳞癌预后的关系。结果食管鳞癌组TIM-3阳性表达率高于对照组,FOXA2阳性表达率低于对照组(P<0.05);不同TNM分期、有无淋巴结转移及不同浸润深度的食管鳞癌患者TIM-3、FOXA2表达比较,差异有统计学意义(P<0.05);食管鳞癌组随访期间生存68例,死亡19例,死亡患者TIM-3阳性表达率高于生存患者,FOXA2阳性表达率低于生存患者(P<0.05);Kaplan-Meier生存曲线显示,TIM-3、FOXA2阴性表达患者总生存率较阳性表达患者高(P<0.05);Cox单因素回归分析显示,TNM分期、淋巴结转移、浸润深度、TIM-3、FOXA2是影响食管鳞癌患者预后的危险因素(P<0.05);Cox多因素分析显示,TNM分期、淋巴结转移、TIM-3、FOXA2是影响食管鳞癌患者预后的独立危险因素(P<0.05)。结论食管鳞癌组织中存在TIM-3、FOXA2表达异常,且与TNM分期、淋巴结转移、浸润深度及预后密切相关。 展开更多
关键词 食管鳞癌 T细胞免疫球蛋白黏蛋白分子3 叉头框蛋白A2 临床病理特征 预后
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支线集装箱船冷藏插座箱布置研究
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作者 陈聪教 欧阳春龙 +2 位作者 马睿博 焦海涛 王少华 《广东造船》 2024年第1期100-103,共4页
冷藏插座箱是支线集装箱船电气系统的重要组成部分。本文总结了适用于不同结构形式、不同区域空间的几种冷藏插座箱的布置方法。同时,综合考虑满足法规、通道宽度、操作、检修、电缆敷设和防磨损等方面的情况,归纳了冷藏插座箱的布置要... 冷藏插座箱是支线集装箱船电气系统的重要组成部分。本文总结了适用于不同结构形式、不同区域空间的几种冷藏插座箱的布置方法。同时,综合考虑满足法规、通道宽度、操作、检修、电缆敷设和防磨损等方面的情况,归纳了冷藏插座箱的布置要点和注意事项,为后续支线集装箱船的冷藏插座箱布置提供参考。 展开更多
关键词 支线集装箱船 冷藏插座箱布置 通道宽度 电缆敷设
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LNG运输船液货围护系统次层绝缘箱质量跟踪系统
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作者 徐军 赵亮 马文昭 《造船技术》 2024年第2期47-51,92,共6页
次层绝缘箱是液化天然气(Liquefied Natural Gas, LNG)运输船液货围护系统的重要组成部分。阐述次层绝缘箱的箱体组装、胶条测试和质量风险,提出基于专用的环氧树脂涂胶流水线的次层绝缘箱质量跟踪系统,为LNG运输船液货围护系统提供安... 次层绝缘箱是液化天然气(Liquefied Natural Gas, LNG)运输船液货围护系统的重要组成部分。阐述次层绝缘箱的箱体组装、胶条测试和质量风险,提出基于专用的环氧树脂涂胶流水线的次层绝缘箱质量跟踪系统,为LNG运输船液货围护系统提供安全保障。 展开更多
关键词 LNG运输船 液货围护系统 次层绝缘箱 质量跟踪系统
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Shear deformable finite beam elements for composite box beams 被引量:2
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作者 Nam-Il Kim Dong-Ho Choi 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2014年第2期223-240,共18页
The shear deformable thin-walled composite beams with closed cross-sections have been developed for coupled flexural, torsional, and buckling analyses. A theoretical model applicable to the thin-walled laminated compo... The shear deformable thin-walled composite beams with closed cross-sections have been developed for coupled flexural, torsional, and buckling analyses. A theoretical model applicable to the thin-walled laminated composite box beams is presented by taking into account all the structural couplings coming from the material anisotropy and the shear deformation effects. The current composite beam includes the transverse shear and the restrained warping induced shear deformation by using the first-order shear deformation beam theory. Seven governing equations are derived for the coupled axial-flexural-torsional-shearing buckling based on the principle of minimum total potential energy. Based on the present analytical model, three different types of finite composite beam elements, namely, linear, quadratic and cubic elements are developed to analyze the flexural, torsional, and buckling problems. In order to demonstrate the accuracy and superiority of the beam theory and the finite beam elements developed by this study,numerical solutions are presented and compared with the results obtained by other researchers and the detailed threedimensional analysis results using the shell elements of ABAQUS. Especially, the influences of the modulus ratio and the simplified assumptions in stress-strain relations on the deflection, twisting angle, and critical buckling loads of composite box beams are investigated. 展开更多
关键词 thin-walled Composite box beam Deflection Twisting angle Buckling load Shear deformation
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