Two-photon luminescence with near-infrared(NIR)excitation of upconversion nanoparticles(NPs)is of great importance in biological imaging due to deep penetration in high-scattering tissues,low auto-luminescence and goo...Two-photon luminescence with near-infrared(NIR)excitation of upconversion nanoparticles(NPs)is of great importance in biological imaging due to deep penetration in high-scattering tissues,low auto-luminescence and good sectioning ability.Unfortunately,common two-photon luminescence is in visible band with an extremely high exciation power density,which limits its application.Here,we synthesized NaYF_(4):Yb/Tm@NaYF_(4)upconversion NPs with strong twophoton NIR emission and a low excitation power density.Furthermore,NaYF_(4):Yb/Tm@NaYF_(4)@SiO_(2)@OTMS@F127 NPs with high chemical stability were obtained by a modified multilayer coating method which was applied to upconversion NPs for thefirst time.In addition,it is shown that the as-prepared hydrophillic upconversion NPs have great biocompatibility and kept stable for 6 hours during in vivo whole-body imaging.The vessels with two-photon luminescence were clear even under an excitation power density as low as 25mW/cm^(2).Vivid visualizations of capillaries and vessels in a mouse brain were also obtained with low background and high contrast.Because of cheaper instruments and safer power density,the NIR two-photon luminescence of NaYF_(4):Yb/Tm@NaYF_(4)upconversion NPs could promote wider application of two-photon technology.The modified multilayer coating method could be widely used for upconversion NPs to increase the stable time of the in vivo circulation.Our work possesses a great potential for deep imaging and imaging-guided treatment in the future.展开更多
背景与目的:三维能量多普勒超声血管成像(three-dimensional power Doppler angiography,3D-PDA)是一种新的血流成像技术,但在甲状腺领域的研究尚不多见。该研究观察甲状腺结节的3D-PDA特征,探讨3D-PDA对甲状腺良恶性结节的鉴别诊断价...背景与目的:三维能量多普勒超声血管成像(three-dimensional power Doppler angiography,3D-PDA)是一种新的血流成像技术,但在甲状腺领域的研究尚不多见。该研究观察甲状腺结节的3D-PDA特征,探讨3D-PDA对甲状腺良恶性结节的鉴别诊断价值。方法:采用3D-PDA对甲状腺结节及周边血流进行三维重建。分析血流分布、血管数量、血管走行是否规则、空间分布是否对称、结节内是否存在局限性血流丰富及周边实质是否存在局限性血流丰富。分析甲状腺结节的3D-PDA特征,比较良恶性结节之间的差异。结果:在103个甲状腺结节中,良性50个(48.5%),恶性53个(51.5%)。在3D-PDA中,血管走行不规则、空间分布不对称、结节内局限性血流丰富及周边实质局限性血流丰富诊断甲状腺恶性结节的灵敏度分别为64.2%、56.0%、54.7%和60.4%,特异度分别为96.0%、88.0%、96.0%和94.0%。3D-PDA诊断甲状腺恶性结节的灵敏度、特异度、阳性预测值、阴性预测值和准确率分别为83.0%、94.0%、93.6%、83.9%和90.3%。结论:3D-PDA可以更好地观察甲状腺结节的血流分布及走行情况,对甲状腺结节的诊断有重要意义。展开更多
目的:探讨经阴道三维能量成像(3D-CPA)检测宫颈癌的血流与miR-455相对表达量的关系。方法:采用实时荧光定量PCR技术检测68例宫颈癌和49例慢性宫颈炎组织中miR-455的表达。用3D-CPA成像对宫颈血流进行检测并计算血管指数(VI),最后进行比...目的:探讨经阴道三维能量成像(3D-CPA)检测宫颈癌的血流与miR-455相对表达量的关系。方法:采用实时荧光定量PCR技术检测68例宫颈癌和49例慢性宫颈炎组织中miR-455的表达。用3D-CPA成像对宫颈血流进行检测并计算血管指数(VI),最后进行比较分析。结果:慢性宫颈炎组宫颈血流不丰富,VI为(0.439±0.181)条/cm3;宫颈癌组宫颈血流丰富、杂乱,VI为(0.940±0.447)条/cm3。宫颈癌组VI高于慢性宫颈炎组,P<0.01。宫颈癌组织内miR-455的表达量低于慢性宫颈炎组(1.17±0.59 vs 2.58±0.83,P<0.01)。宫颈癌组织内miR-455的表达水平与VI呈负相关(r=-0.295,P=0.014)。结论:miR-455低表达可能促进宫颈癌的血管生成,是一个潜在的抑癌基因。展开更多
目的探讨三维能量多普勒(three-dimensional power doppler angiography,3D-PDA)血流参数测定对鉴别阴道出血子宫内膜良性与癌前病变的临床价值。方法选择65例阴道出血临床怀疑子宫内膜病变的患者行3D-PDA检查,定量检测子宫内膜容积(V)...目的探讨三维能量多普勒(three-dimensional power doppler angiography,3D-PDA)血流参数测定对鉴别阴道出血子宫内膜良性与癌前病变的临床价值。方法选择65例阴道出血临床怀疑子宫内膜病变的患者行3D-PDA检查,定量检测子宫内膜容积(V),血流参数血流指数(FI),血管指数(VI)及血管-血流指数(VFI),以子宫内膜病理学结果为标准,评价其诊断符合率。应用接受者操作特性曲线(ROC曲线)比较各项指标的诊断价值。结果子宫内膜癌前病变三维彩色多普勒能量图示血供丰富及较丰富,子宫内膜良性病变大多彩色多普勒能量图示血供不丰富。脉冲重复频率PRF:0.9 MHz时,良性病变组FI、VI和VFI分别为:3.98%±3.42%、21.13±3.15和0.643±0.328癌前病变组FI、VI和VFI分别为:5.903%±2.761%、28.964±2.446和1.705±0.928;两组比较差异有统计学意义(P<0.05);不同脉冲重复频率PRF:0.9 MHz/0.6MHz,VI、FI、VFI值差异有统计学意义(P<0.05);两组子宫内膜体积(V)比较,差异无统计学意义;3D-PDA检测的灵敏性88.9%,特异性100%。VI、FI和VFI的ROC曲线下面积(AUC)分别为:0.851、0.772、0.950,其中VFI值AUC最大,VFI截断值取0.81时,灵敏性为94.4%,特异性为12.8%。结论通过对病变子宫内膜进行3D-PDA及其血流定量检测,并结合子宫内膜声像图特点,能对子宫内膜良性与癌前病变鉴别提供有价值的信息。展开更多
基金This work is partially supported by National Key Research and Development Program of China(Grant No.2018YFC1407503)the Fundamental Research Funds for the Central Universities(2018FZA5001)The National Natural Science Foundation of China(Grant No.11621101).
文摘Two-photon luminescence with near-infrared(NIR)excitation of upconversion nanoparticles(NPs)is of great importance in biological imaging due to deep penetration in high-scattering tissues,low auto-luminescence and good sectioning ability.Unfortunately,common two-photon luminescence is in visible band with an extremely high exciation power density,which limits its application.Here,we synthesized NaYF_(4):Yb/Tm@NaYF_(4)upconversion NPs with strong twophoton NIR emission and a low excitation power density.Furthermore,NaYF_(4):Yb/Tm@NaYF_(4)@SiO_(2)@OTMS@F127 NPs with high chemical stability were obtained by a modified multilayer coating method which was applied to upconversion NPs for thefirst time.In addition,it is shown that the as-prepared hydrophillic upconversion NPs have great biocompatibility and kept stable for 6 hours during in vivo whole-body imaging.The vessels with two-photon luminescence were clear even under an excitation power density as low as 25mW/cm^(2).Vivid visualizations of capillaries and vessels in a mouse brain were also obtained with low background and high contrast.Because of cheaper instruments and safer power density,the NIR two-photon luminescence of NaYF_(4):Yb/Tm@NaYF_(4)upconversion NPs could promote wider application of two-photon technology.The modified multilayer coating method could be widely used for upconversion NPs to increase the stable time of the in vivo circulation.Our work possesses a great potential for deep imaging and imaging-guided treatment in the future.
文摘背景与目的:三维能量多普勒超声血管成像(three-dimensional power Doppler angiography,3D-PDA)是一种新的血流成像技术,但在甲状腺领域的研究尚不多见。该研究观察甲状腺结节的3D-PDA特征,探讨3D-PDA对甲状腺良恶性结节的鉴别诊断价值。方法:采用3D-PDA对甲状腺结节及周边血流进行三维重建。分析血流分布、血管数量、血管走行是否规则、空间分布是否对称、结节内是否存在局限性血流丰富及周边实质是否存在局限性血流丰富。分析甲状腺结节的3D-PDA特征,比较良恶性结节之间的差异。结果:在103个甲状腺结节中,良性50个(48.5%),恶性53个(51.5%)。在3D-PDA中,血管走行不规则、空间分布不对称、结节内局限性血流丰富及周边实质局限性血流丰富诊断甲状腺恶性结节的灵敏度分别为64.2%、56.0%、54.7%和60.4%,特异度分别为96.0%、88.0%、96.0%和94.0%。3D-PDA诊断甲状腺恶性结节的灵敏度、特异度、阳性预测值、阴性预测值和准确率分别为83.0%、94.0%、93.6%、83.9%和90.3%。结论:3D-PDA可以更好地观察甲状腺结节的血流分布及走行情况,对甲状腺结节的诊断有重要意义。
文摘目的:探讨经阴道三维能量成像(3D-CPA)检测宫颈癌的血流与miR-455相对表达量的关系。方法:采用实时荧光定量PCR技术检测68例宫颈癌和49例慢性宫颈炎组织中miR-455的表达。用3D-CPA成像对宫颈血流进行检测并计算血管指数(VI),最后进行比较分析。结果:慢性宫颈炎组宫颈血流不丰富,VI为(0.439±0.181)条/cm3;宫颈癌组宫颈血流丰富、杂乱,VI为(0.940±0.447)条/cm3。宫颈癌组VI高于慢性宫颈炎组,P<0.01。宫颈癌组织内miR-455的表达量低于慢性宫颈炎组(1.17±0.59 vs 2.58±0.83,P<0.01)。宫颈癌组织内miR-455的表达水平与VI呈负相关(r=-0.295,P=0.014)。结论:miR-455低表达可能促进宫颈癌的血管生成,是一个潜在的抑癌基因。
