The correlation of secondary brain injury with thromboxane A_2(TXA_2) and prostacy- clin (PGI_2) levels following head injury was studied in rats and patients.Thromboxane B_2 (TXB_2) and 6-keto-PGF_(1α) in rat brain ...The correlation of secondary brain injury with thromboxane A_2(TXA_2) and prostacy- clin (PGI_2) levels following head injury was studied in rats and patients.Thromboxane B_2 (TXB_2) and 6-keto-PGF_(1α) in rat brain homogenate and TXB_2 in cerebral spinal fluid (CSF) of patients with severe head injury were determined by RIA.The effects of ligustrazini hydrochlo- rioi were also tested.The results showed that the concentration of TXB_2 and 6-keto-PGF_(1α) in rat brain was progressively increased within 3h after injury (P<0.01),and the ratio between TXB_2 and 6-keto-PGF_(1α)(T/K value) increased during the early stage after injury,along with the tissue damage aggravation.After the use of ligustrazini hydrochlorioi,TXB_2 content in brain decreased sharply accompanied with a drop in T/K value.Moreover,TXB_2 content in CSF increased within 7 d after injury (P<0.01),and it reached its peak value on the 3rd day. This could indicate that the metabolic imbalance between TXA_2 and PGI_2 might be one of the important factors in the development of secondary brain injury,and ligustrazini hydrochlorioi proved to have a protective effect on the brain tissue by normalizing the metabolism of TXA_2 and PGI_2.In addition,the metabolic disorders of TXA_2 in the brain tissue of head injured pa- tients have much to do with the outcome of the patients.展开更多
Objective: To explore the effects of altitude hypoxia on septic pulmonary injury (3080 m above sea level). Methods : A model of pyosepticemia was established by cecal ligation and puncture. Thromboxane A2 (TXA2),prost...Objective: To explore the effects of altitude hypoxia on septic pulmonary injury (3080 m above sea level). Methods : A model of pyosepticemia was established by cecal ligation and puncture. Thromboxane A2 (TXA2),prostacyclin (PGI2) of the venous plasma and leucine-enkephalins (L-ENK) in the arterial walls were measured using radioiizimunoassay in 31 rabbits. Mouse morrality was observed. Results: 24 h mouse accumulative mortality (97. 1% )was greater than that of control (35%). TXB2 content was 4 times as much as in control group and 6-keto-PGF1α level was elevated too. These changes of arachidonic metabolism could be blorked by indomethacin. The contents of LENK in arterial walls of puhiionary, mesenteric and renal arteries dropped significantly in comparison with control group (P<0. 01 ). The albumin content in the bronchoalveolar lavage fluid rase significantly in comparison with control group (P<0. 01 ). Conclusion : The results demonstrate that the metabolites of cyclcoxygenase are not the major mediator of puhiionary permeability in the experiment. The alteration of TXA2 and L-ENK contents may be one of the mechanisms, by which the puhiionary artery pressure and pulmonary vascular resistance are increased during sepsis.展开更多
文摘The correlation of secondary brain injury with thromboxane A_2(TXA_2) and prostacy- clin (PGI_2) levels following head injury was studied in rats and patients.Thromboxane B_2 (TXB_2) and 6-keto-PGF_(1α) in rat brain homogenate and TXB_2 in cerebral spinal fluid (CSF) of patients with severe head injury were determined by RIA.The effects of ligustrazini hydrochlo- rioi were also tested.The results showed that the concentration of TXB_2 and 6-keto-PGF_(1α) in rat brain was progressively increased within 3h after injury (P<0.01),and the ratio between TXB_2 and 6-keto-PGF_(1α)(T/K value) increased during the early stage after injury,along with the tissue damage aggravation.After the use of ligustrazini hydrochlorioi,TXB_2 content in brain decreased sharply accompanied with a drop in T/K value.Moreover,TXB_2 content in CSF increased within 7 d after injury (P<0.01),and it reached its peak value on the 3rd day. This could indicate that the metabolic imbalance between TXA_2 and PGI_2 might be one of the important factors in the development of secondary brain injury,and ligustrazini hydrochlorioi proved to have a protective effect on the brain tissue by normalizing the metabolism of TXA_2 and PGI_2.In addition,the metabolic disorders of TXA_2 in the brain tissue of head injured pa- tients have much to do with the outcome of the patients.
文摘Objective: To explore the effects of altitude hypoxia on septic pulmonary injury (3080 m above sea level). Methods : A model of pyosepticemia was established by cecal ligation and puncture. Thromboxane A2 (TXA2),prostacyclin (PGI2) of the venous plasma and leucine-enkephalins (L-ENK) in the arterial walls were measured using radioiizimunoassay in 31 rabbits. Mouse morrality was observed. Results: 24 h mouse accumulative mortality (97. 1% )was greater than that of control (35%). TXB2 content was 4 times as much as in control group and 6-keto-PGF1α level was elevated too. These changes of arachidonic metabolism could be blorked by indomethacin. The contents of LENK in arterial walls of puhiionary, mesenteric and renal arteries dropped significantly in comparison with control group (P<0. 01 ). The albumin content in the bronchoalveolar lavage fluid rase significantly in comparison with control group (P<0. 01 ). Conclusion : The results demonstrate that the metabolites of cyclcoxygenase are not the major mediator of puhiionary permeability in the experiment. The alteration of TXA2 and L-ENK contents may be one of the mechanisms, by which the puhiionary artery pressure and pulmonary vascular resistance are increased during sepsis.
