Objective:To examine the effect of water extract of Thunbergia laurifolia on hepatic insulin resistance in high-fat diet-induced obese mice.Methods:High-fat diet with 45 kcal%lard fat was used for obesity induction in...Objective:To examine the effect of water extract of Thunbergia laurifolia on hepatic insulin resistance in high-fat diet-induced obese mice.Methods:High-fat diet with 45 kcal%lard fat was used for obesity induction in ICR mice.The mice were fed with high-fat diet for 16 weeks,and during the last 8 weeks,they were treated with 200 mg/kg/day of water extracts from Thunbergia laurifolia leaf,stem and flower.Serum biochemistry,liver histology,and protein expression were examined after the treatment.Results:Extracts from all of the three parts of Thunbergia laurifolia significantly alleviated hyperglycemia,hyperlipidemia,hyperinsulinemia,and hyperleptinemia.The stem and flower extracts improved glucose tolerance.All of the extracts significantly reduced serum TNFαand monocyte chemoattractant protein-1 levels.Liver weight,triglyceride levels,and lipid accumulation were also decreased.Moreover,hepatic glucose-6-phosphatase level was significantly decreased,while the levels of PPARα,phosphorylated AMPK,and phosphorylated Akt were significantly increased with treatment of Thunbergia laurifolia extracts.Conclusions:Thunbergia laurifolia extracts can ameliorate hepatic insulin resistance in high-fat diet-induced obese mice by improving glucose and lipid homeostasis,which may be associated with stimulating phosphorylation of AMPK and Akt pathways.展开更多
[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,a...[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.展开更多
Background: Thunbergia erecta (Acanthaceae) is the most abundant medicinal plant in different parts of Bangladesh where it is known as “nilghonta”. It has been used as traditional medicine for insomnia, depression a...Background: Thunbergia erecta (Acanthaceae) is the most abundant medicinal plant in different parts of Bangladesh where it is known as “nilghonta”. It has been used as traditional medicine for insomnia, depression and anxiety management. However, no scientific evidence of T. erecta belonging to neuropharmacological activity has been reported. The aim of present study was to investigate in vivo sedative and anxiolytic activities of methanol extract from the leaves of T. erecta in Swiss Albino mice. Methods: Sedative activity of METE was investigated using open field, hole cross and thiopental sodium-induced sleeping time test model whereas anxiolytic activity was screened by elevated-plus maze, light-dark box, hole-board and marble-burying test method in mice at 200 and 400 mg/kg doses. The acute toxicity study and phytochemical analysis of METE also carried out. Diazepam used as the positive control for the following behavioral pharmacology test. Results: METE exhibited significant (p Conclusion: The experimental result indicates T. erecta contains phytoconstituents that possess sedative and anxiolytic activity which traditionally used in insomnia, depression and anxiety management.展开更多
Objective:To compare cytotoxic and thrombolytic activity of crude methanolic extract of Cissus pentagona(C.pentagona)and Thunbergia grandiflora Roxb.(T.grandiflora)leaves.Methods:The screening of cytotoxic activity wa...Objective:To compare cytotoxic and thrombolytic activity of crude methanolic extract of Cissus pentagona(C.pentagona)and Thunbergia grandiflora Roxb.(T.grandiflora)leaves.Methods:The screening of cytotoxic activity was done by using brine shrimp lethality bioassay while the thrombolytic activity was evaluated by using the in vitro clot lysis model.In brief,venous blood from five healthy volunteers was allowed to form clots which were weighed and treated with the tested plant materials to disrupt the clots.Weight of clot before and after treatment provided a percentage of clot lysis and the results with streptokinase as positive control and water as negative control were compared.Results:Moderate cytotoxicity was found for both methanol extracts,and it was compared with the standard drug vincristine sulfate in the brine shrimp bioassay.In the present study,the LC50 values of the methanol crude extract of C.pentagona as well as T.grandiflora and vincristine sulfate were 291.33,243.37 and 12.59μg/mL,respectively.In thrombolytic study,it was found that C.pentagona and T.grandiflora showed(24.27±2.61)%and(19.56±2.98)%of clot lysis,respectively.Among the herbs studied,C.pentagona showed very significant(P<0.001)percentage of clot lysis than T.grandiflora,compared with reference drug streptokinase[(63.54±2.61)%].Conclusions:The results of the study demonstrated that the leaf of the plants contains preliminary cytotoxic effect on brine shrimp and promising thrombolytic activity in vitro when it is tested on human blood.However,further study is needed to evaluate its potential as a thrombolytic agent.展开更多
目的:明确为害山牵牛的叶部病害的病原菌,为其防治奠定基础。方法:采用常规组织分离法对山牵牛炭疽病的病原菌进行分离,利用形态学特征和ITS r DNA序列分析法鉴定该病原菌的分类地位。结果:该病原鉴定为胶孢炭疽菌Colletotrichum gloeos...目的:明确为害山牵牛的叶部病害的病原菌,为其防治奠定基础。方法:采用常规组织分离法对山牵牛炭疽病的病原菌进行分离,利用形态学特征和ITS r DNA序列分析法鉴定该病原菌的分类地位。结果:该病原鉴定为胶孢炭疽菌Colletotrichum gloeosporioides。结论:该病原菌危害山牵牛造成其叶部病害,在国内为首次报道。展开更多
基金supported by the research grant from the National Research Council of Thailand(Contract number 37/2560)
文摘Objective:To examine the effect of water extract of Thunbergia laurifolia on hepatic insulin resistance in high-fat diet-induced obese mice.Methods:High-fat diet with 45 kcal%lard fat was used for obesity induction in ICR mice.The mice were fed with high-fat diet for 16 weeks,and during the last 8 weeks,they were treated with 200 mg/kg/day of water extracts from Thunbergia laurifolia leaf,stem and flower.Serum biochemistry,liver histology,and protein expression were examined after the treatment.Results:Extracts from all of the three parts of Thunbergia laurifolia significantly alleviated hyperglycemia,hyperlipidemia,hyperinsulinemia,and hyperleptinemia.The stem and flower extracts improved glucose tolerance.All of the extracts significantly reduced serum TNFαand monocyte chemoattractant protein-1 levels.Liver weight,triglyceride levels,and lipid accumulation were also decreased.Moreover,hepatic glucose-6-phosphatase level was significantly decreased,while the levels of PPARα,phosphorylated AMPK,and phosphorylated Akt were significantly increased with treatment of Thunbergia laurifolia extracts.Conclusions:Thunbergia laurifolia extracts can ameliorate hepatic insulin resistance in high-fat diet-induced obese mice by improving glucose and lipid homeostasis,which may be associated with stimulating phosphorylation of AMPK and Akt pathways.
基金Supported by Undergraduate Innovation and Entrepreneurship Project of Department of Education of Guangxi Zhuang Autonomous Region(No:201813643063).
文摘[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.
文摘Background: Thunbergia erecta (Acanthaceae) is the most abundant medicinal plant in different parts of Bangladesh where it is known as “nilghonta”. It has been used as traditional medicine for insomnia, depression and anxiety management. However, no scientific evidence of T. erecta belonging to neuropharmacological activity has been reported. The aim of present study was to investigate in vivo sedative and anxiolytic activities of methanol extract from the leaves of T. erecta in Swiss Albino mice. Methods: Sedative activity of METE was investigated using open field, hole cross and thiopental sodium-induced sleeping time test model whereas anxiolytic activity was screened by elevated-plus maze, light-dark box, hole-board and marble-burying test method in mice at 200 and 400 mg/kg doses. The acute toxicity study and phytochemical analysis of METE also carried out. Diazepam used as the positive control for the following behavioral pharmacology test. Results: METE exhibited significant (p Conclusion: The experimental result indicates T. erecta contains phytoconstituents that possess sedative and anxiolytic activity which traditionally used in insomnia, depression and anxiety management.
基金Supported by Department of Pharmacy,International Islamic University Chittagong,Chittagong,Bangladesh(Grant No.Pharmacy P&D 59/04-10).
文摘Objective:To compare cytotoxic and thrombolytic activity of crude methanolic extract of Cissus pentagona(C.pentagona)and Thunbergia grandiflora Roxb.(T.grandiflora)leaves.Methods:The screening of cytotoxic activity was done by using brine shrimp lethality bioassay while the thrombolytic activity was evaluated by using the in vitro clot lysis model.In brief,venous blood from five healthy volunteers was allowed to form clots which were weighed and treated with the tested plant materials to disrupt the clots.Weight of clot before and after treatment provided a percentage of clot lysis and the results with streptokinase as positive control and water as negative control were compared.Results:Moderate cytotoxicity was found for both methanol extracts,and it was compared with the standard drug vincristine sulfate in the brine shrimp bioassay.In the present study,the LC50 values of the methanol crude extract of C.pentagona as well as T.grandiflora and vincristine sulfate were 291.33,243.37 and 12.59μg/mL,respectively.In thrombolytic study,it was found that C.pentagona and T.grandiflora showed(24.27±2.61)%and(19.56±2.98)%of clot lysis,respectively.Among the herbs studied,C.pentagona showed very significant(P<0.001)percentage of clot lysis than T.grandiflora,compared with reference drug streptokinase[(63.54±2.61)%].Conclusions:The results of the study demonstrated that the leaf of the plants contains preliminary cytotoxic effect on brine shrimp and promising thrombolytic activity in vitro when it is tested on human blood.However,further study is needed to evaluate its potential as a thrombolytic agent.
文摘目的:明确为害山牵牛的叶部病害的病原菌,为其防治奠定基础。方法:采用常规组织分离法对山牵牛炭疽病的病原菌进行分离,利用形态学特征和ITS r DNA序列分析法鉴定该病原菌的分类地位。结果:该病原鉴定为胶孢炭疽菌Colletotrichum gloeosporioides。结论:该病原菌危害山牵牛造成其叶部病害,在国内为首次报道。
