普通菜豆(Phaseolus vulgaris L.)AOX基因家族鉴定与表达分析

交替氧化酶(Alternative oxidase,AOX)是一种末端氧化酶,在维持植物正常生长代谢速率和代谢平衡以及抵御逆境胁迫中发挥了一定作用。AOX家族成员已经在很多植物物种中被鉴定,但是在普通菜豆(Phaseolus vulgaris L.)中却未见报道。研究对普通菜豆中AOX家族成员进行了鉴定和分析,并进一步解析了AOX基因家族成员的功能及调控机制。结果表明,基于普通菜豆参考基因组中共挖掘得到了4个AOX家族成员,通过进化关系、保守基序(Motifs)、基因结构分析,将各成员划分成4个亚族。此外,顺式作用元件、共线性与盐胁迫下的表达量分析结果表明,AOX成员可能在植物的非生物胁迫响应中发挥了作用。为后续深入研究普通菜豆中AOX成员的功能奠定了理论基础。

Effect of Biostimulants Based on Natural Products on the Growth and Nutritional Value of Bean (Phaseolus vulgaris L.)

Beans (Phaseolus vulgaris L.) are widely grown in Cameroon and play a key role in the fight against food insecurity, malnutrition and poverty. However, its cultivation encounters problems due to abiotic and biotic stresses, which leads to the use of synthetic fertilizers and pesticides, which cause significant damage to the environment and human health due to the presence of synthetics residues in the seeds, pods and in the leaves that are eaten. Promoting the use of natural products is becoming a necessity for organic and eco-responsible agriculture that limits contamination problems and improves people’s purchasing power. This study aims to assess the effect of biostimulants based on natural products on the growth and nutritional value of common bean (Phaseolus vulgaris L.). Bean seedlings from white variety (MEX-142) and red variety (DOR-701) were treated every seven days in the field from their pre-emergence, emergence and growth to their maturation under a randomized block experimental design. Six treatments and three repetitions with the biostimulants based on natural products and controls were thus performed and the agromorphological parameters were measured. After 120 days, the contents of growth biomarkers and defense-related enzymes were evaluated in leaves, while the contents of macromolecules, minerals and antinutrients were evaluated in seeds. These biostimulants significantly increased (P P < 0.0001) of antinutrients including oxalates, phytates, tannins and saponins in seeds compared to controls (T+ and T−). Treatment with biostimulants, in particular BS4, improves the performance of bean plants in the field as well as the biofortification of seeds regardless of the variety.

Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

Objective:To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of Thymus vulgaris L(T.vulgaris)by gas chromatography-mass spectrometry(GC-MS).Methods:The shade dried leaves powder was extracted with methanol by using Soxhlet extractor.Methanol crude extracts of T.vulgaris and the derived fractions of hexane,chloroform,ethyl acetate and butanol were obtained.Results:Qualitative analyses of various organic crude extracts of T.vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds.Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important.Further,the T.vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation.The crude extracts were prepared from the powder leaves of T.vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis.Conclusions:All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T.vulgaris are biologically active molecules.Thus the identification of a good number of compounds in various crude extracts of T.vulgaris might have some ecological role.

The protection of Thymus vulgaris leaves alcoholic extract against hepatotoxicity of alcohol in rats

Objective:This study was performed to investigate the protective effect of Thymus vulgaris(T.vulgaris) leaves 70%alcoholic extract against alcohol-mediate hepatotoxicity rats.Methods:The protective effect of extract was investigated at dose of 500 mg/kg/day orally against alcohol-mediate hepatotoxicity using adult male Wister albino rats during 21 days.Protective effect of T.vulgaris extract was evaluated comparing with silymarin standard drug al recommended dose(25 mg/kg/day) orally for 21 days.Results:Alcohol-mediate hepatotoxicity rats(alcohol-control) showed hepatocytes distortion represented as marked increment on liver biomarkers;alkaline phosphatase(ALP),aspartate transaminase(AST) and alanine transaminase(ALT) activities,as well as pronounced reduction on total protein and its fractions albumin and globulin corresponding to normal ranges.Addition to oxidative stress status as depletion on glutathione concentration,catalase(CAT),superoxide dismutase(SOD),glutathione reductase(GR),glutathione-S-transferase(GST) and glutathione peroxidase(GPx)activities,concurrence with augmentation oxidative stress parameters;malondyaldchydc(MDA) and hydrogen peroxide(H_2O_2) concentrations comparing to normal values.Alcohol administration elevated total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) comparing to normal ranges.Co-administration T.vulgaris extract with alcohol showed protective effect on hepatocytes manifested as minimizing on ALP.AST and ALT activities and increment on total protein,albumin and globulin production compared to alcohol-control.Antioxidant enzymes activities;CAT.SOD.GR,GST and GPx were significantly magnified,while MDA and H_2O_2 concentration were lessened corresponding to alcohol-control.Also,lipid profile was markedly improved and risk ratio was lowered compared to alcohol-control.These results were confirmed by normalization of degenerated and fibrotic liver tissue as of alcohol-control.Conclusion:T.vulgaris extract appeared hepatoprotective,hypolipidemic and antioxidant activities on alcohol-mediate hepatotoxicity rats compared to silymarin.

Study of total phenol,flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris

Objective:To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol,flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar,Nizwa.Sultanate of Oman.Methods:The leave sample was extracted with methanol and evaporated.Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities.The prepare hexane,chloroform,ethyl acetate,hutanol and methanol crude extracts were used for their evaluation of total phenol,flavonoids contents and phytochemical screening study.The established conventional metliods were used for quantitative determination of total phenol,flavonoids contents and phytochemical screening.Results:Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids,saponins and steroids compounds.The result for total phenol content was the highest in hutanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.Conclusions:The crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.

Inhibitory effect of Thymus vulgaris extract on memory impairment induced by scopolamine in rat

Objective: To investigate the effect of Thymus vulgaris(T. vulgaris) on learning and memory functions in scopolamine-induced memory deficit in rats. Memory enhancing activity in scopolamine-induced amnesic rats was investigated by assessing the Morris water maze and passive avoidance paradigm.Methods: A total of 42 male Wistar rats were divided into 6 equal groups as follow:control group: received water, scopolamine treated group: received scopolamine 1 mg/kg for 15 days, two scopolamine + T. vulgaris treated groups: received scopolamine and T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days, two intact groups:received T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days.Results: Administration of T. vulgaris extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and Morris water maze test.Conclusions: T. vulgaris extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of Alzheimer’s disease.

Chemical Composition, Antibacterial and Antioxidant Activities of Thyme Essential Oil (<i>Thymus vulgaris</i>)

Herbal medicine from natural resources plays an important role as antibacterial and antioxidant agents. The present investigation was designed to evaluate the antibacterial and antioxidant properties of thyme (Thymus vulgaris L.) essential oil (TEO) and/or chitosan (CH) in vitro. Results indicated that TEO exhibited high radical scavenging activity (RSA) toward DPPH, ABTS, linoleic acid deterioration and iron chelation activity. TEO exhibited high amount of total phenolic compounds (TPC) related to its terpenes. The TPC of TEO was 177.3 mg GAE g-1 demonstrated 149.8 μmol of TE g-1 DPPH-RSA and 192.4 μmol of TE g-1 ABTS-RSA. The antioxidant capacity of TEO exhibited 68.9% reduction when evaluated by β-carotene bleaching assay. The reducing power activity related to iron chelation was 142.8 μmol of AAE g-1. The TEO exhibited a high content of Thymol (41.04%) as major compound over 14 identified components by GC-MS analysis followed by 1,8-Cineole (14.26%), γ-Terpinene (12.06%), p-Cymene (10.50%) and α-Terpinene (9.22%). TEO exhibited antimicrobial activity in vitro and MIC noticed that TEO was efficiently affected pathogens in vitro. Indeed, CH exhibited negligible or very low antimicrobial activity. In conclusion, both investigated TEO and TEO-CHmix have strong antibacterial activity against many pathogenic bacteria and need exploitation as an alternative source of natural antibacterial and antioxidant agents for potential applications.

Essential oil of <i>Thymus vulgaris</i>L. and <i>Rosmarinus officinalis</i>L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens

The essential oil composition of Thymus vulgaris L. and Rosmarinus officinalis L. endemic to France were determined by GC and GC-MS. Oils were assessed for their cytotoxic, antioxidant and antimicrobial activity. 31 and 37 different compounds were identified representing 99.64% and 99.38% of the thyme and rosemary oils respectively, where oxygenated monoterpenes constituted the main chemical class. Thymol (41.33%) and 1.8-cineole (24.10%) were identified as the main constituents of T. vulgaris L. and R. officinalis L., respectively. Essential oils (EOs) of selected plant species were evaluated for their in vitro cytotoxicity against the human lung adenocarcinoma epithelial cell line (A549). Cytotoxicity was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphynyltetra-zolium bromide] colorimetric assay. Dose-dependent studies revealed IC50 of 8.50 ± 0.01 μg/mL and 10.50 ± 0.01 μg/mL after 72 h on the A549 cells for R. officinalis L. and T. vulgaris L., respectively. Antioxidant activity was determined using a quantitative DPPH (1,1-diphenyl- 2-picryl hydrazyl) assay. Thymus and rosemary EOs exhibited effective radical scavenging capacity with 50% inhibitory concentration (IC50) of 437 ± 5.46 μg/mL and 189 ± 2.38 μg/mL respectively and therefore acts as a natural antioxidant agent. The antimicrobial activity of these species has also been studied against several foodborne pathogens and food isolated Salmonella spp. including S. enteritidis of significant importance. According to the results, T. vulgaris L. showed higher bactericidal effect than those from R. officinalis L. These results suggest that the essential oil from T. vulgaris L. and R. officinalis L. have potential to be used as a natural cytotoxic, antioxidant and antimicrobial agent in food processing.

Chemical composition of essential oil of Thymus vulgaris collected from Saudi Arabian market

Objective: To focus on the analysis of chemical constituents of the Thymus vulgaris L.(locally known as "Zaitra" or "Za'atar"; Family: Lamiaceae) which is available in the market of Saudi Arabia.Methods: The Zaitra oil was analyzed by gas chromatography-mass spectrometry. The mass spectra were compared with the standard spectra available in National Institute Standard and Technique library.Results: The results indicated that the Thymus oil is composed of many chemical compounds including a pinene, thymol and caryophyllene which are biologically active and also used in various diseases.Conclusions: It can be concluded that the Thymus vulgaris due to presence of many bioactive compounds can be used as a new potential source of medicine for the treatment of various types of illness.

Effect of Macronutrients, Cytokinins and Auxins, on <i>in Vitro</i>Organogenesis of <i>Thymus vulgaris</i>L.

The present study reports an efficient protocol for in vitro propagation of Thymus vulgaris L., an aromatic and medicinal plant in Morocco. Initially, we performed in vitro multiplication of Thymus vulgaris explants existing in the laboratory and obtained from micropropagation by shoot tip culture. Afterwards, we have evaluated the effect of six macronutrients. After that, seven cytokinins (Kin, BAP, 2iP, DPU, Adenine, Zeatine and TDZ) in three different concentrations (0.46, 0.93, 2.32 μM) have been evaluated to optimize cultures multiplication and elongation. Moreover, the effect of three auxins (IAA, IBA and NAA) at 0.57 μM, combined to 4 cytokinins (Kin, BAP, DPU and Ad.) at 0.46 μM, on shoot rooting has been studied. Thereby, MS medium has been proved the most favorable for plantlets growing. Also, we found that the addition of certain cytokinins, specifically 0.46 Kin, 0.46 and 0.93 BAP, 0.46 2iP, 0.46 DPU, 0.46 Ad. and 0.46 Zeat., ensures better multiplication and growth of vitroplants. In addition, multiplication and rooting of cultures were well optimized after addition 0.46 Kin + 0.57 IAA or NAA, 0.46 DPU + 0.57 IBA and 0.46 Ad. + 0.57 IBA combinations to the culture medium. Lastly, plantlets with roots were successfully acclimatized to ex-vitro conditions and these latter served as a source to establish in vitro culture again.

The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of <i>Thymus vulgaris</i>

Medicinal plants have played an essential role in the development of human culture. Medicinal plants are resources of traditional medicines and many of the modern medicines are produced indirectly from plants. This study illustrates the importance of traditional and modern medicines in the treatment and management of human diseases and ailments. It has been confirmed by WHO that herbal medicines serve the health needs of about 80 percent of the world’s population;especially for millions of people in the vast rural areas of developing countries. Meanwhile, consumers in developed countries are becoming disillusioned with modern health care and are seeking alternatives. Thymus vulgaris is a species of flowering plant in the mint family Lamiacea. Thymus is a widely used medicinal plant in food and pharmaceutical industries. Among different species of Thymus, Thymus vulgaris is used more than other species in therapeutic dosage forms. In Traditional medicine T. vulgaris is cultivated in many countries by most people especially in rural areas depending on herbal medicines to treat many diseases including inflammation-related ailments such as rheumatism, muscle swelling, insect bites, pains, etc. Also the modern medicine in essential oil of thyme has demonstrated that the compounds have shown anti-inflammatory, antioxidant, antibacterial and antifungal properties. In this review the objective is to consider the past and present value of medicinal plants such as Thymus vulgar is used in traditional and modern medical practices as bioactive natural compounds.

Photosynthetic Gas Exchange and Nitrogen Assimilation in Green Bean Plants Supplied with Two Sources of Silicon

Beans contain a wide range of vitamins,proteins,calcium,and zinc which make them an important food source for many countries.To meet the demand for bean production worldwide,large amounts of fertilizers and pesticides are used.However,the cost of production and environmental impact increases.To produce food sustainably,the use of beneficial nutrients such as silicon as a biostimulant has been proposed.However,information about the effect of different sources of silicon on the metabolism of bean plants is scarce.Bean plants cv.Strike were grown in pots for 60 days and the effect of foliar application of silicon nanoparticles and the silicon-based biostimulant Codasilat 4 concentrations(0,1,2,and 4 mM)on total biomass,yield,photosynthetic pigment concentration,photosynthetic activity,stomatal conductance,transpiration rate,chlorophyll fluorescence,and nitrogen assimilation were evaluated.The results obtained showed that the supply of silicon at a dose of 1 mM functioned as a biostimulant,favoring gas exchange and nitrogen assimilation within the plant,which stimulated growth and yield.The results of this research work allowed a better comprehension of the effects of silicon application through silicon nanoparticles and the biostimulant Codasilon the physiology of green bean plants.

Antiprotozoal Activity of a <i>Thymus vulgaris</i>Methanol Extract and Its Fractions

Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.

Effectiveness of <i>Thymus vulgaris</i>Essential Oil in the Treatment of Skin Infections in Dogs

Currently, there are no data in the literature on the topical treatment with Thymus vulgaris essential oil (EO) in bacterial skin infections of dogs. This study evaluates the effectiveness of Thymus vulgaris EO for topical use in dogs with skin 18 half-breed dogs, affected by skin infections housed in a rescue shelter were studied. The bacteria isolated from these dogs were identified by MALDI-TOF-MS. The susceptibility of the isolated microorganisms to Thymus vulgaris L. EO was estimated in vitro by bacteriological test (CLSI 2015), in comparison to some antimicrobials drugs (amoxicillin-clavulanic acid, doxycy-cline, thiamphenicol and marbofloxacin) and to Citrus bergamia EO. The dogs, divided in two groups at random, were treated topically for 7 days with Thymus vulgaris L. EO (Group 1: n. 10 animals) and Citrus bergamia Risso e Poiteau EO (Group 2: n. 8 animals) respectively. The bacteria isolated were: Staphylococcus pseudintermedius (7 samples), Staphylococcus sciuri (4), ESBL Escherichia coli (3) and Proteus mirabilis (4). In all animals treated with Thymus vulgaris EO, the clinical signs decreased rapidly within 5 days from the administration, with complete remission 7 days after the treatment. No bacterial growth was observed from skin swabs after 7 days of treatment. None of the treated animals showed local or general side effects. The use of Thymus vulgaris EO could be a possible alternative or additional treatment to antibiotics in dermatological infections, particularly in cases refractory to conventional therapy.

超富集植物叶用红菾菜(Beta vulgaris var.cicla L.)及其对Cd的富集特征

超富集植物是重金属污染土壤植物提取修复的基础,超富集植物的筛选一直都是污染土壤植物修复研究的热点及前沿。采用盆栽实验对7种农作物积累Cd的特征进行研究,结果表明,叶用红菾菜对Cd具有较高的富集系数和转移系数。在此基础上,采用盆栽浓度梯度试验对叶用红菾菜对Cd的富集潜力及富集特征进行了系统研究。结果表明,在Cd污染水平为20mg·kg^(-1)条件下,叶用红菾菜地上部Cd含量超过了100mg·kg^(-1)这一国际公认的Cd超积累植物应达到的临界含量标准;且其对Cd的富集系数和转移系数均大于1;同时,与对照相比,植物的生长未受到抑制,这表明叶用红菾菜是一种Cd超积累植物,为Cd污染土壤的植物修复提供了一种新的种质资源。

低温诱导甜菜(Beta vulgaris L.)Ty7Br600基因的Northern blotting和Southern blotting分析

以低温诱导甜菜幼苗为试验材料,以未进行低温诱导的甜菜幼苗为对照,采用α-32P-ATP放射标记按随机引物标记法标记本实验室克隆的Ty7Br600基因,进行Northern杂交。Northern杂交试验结果显示,在低温诱导培养的甜菜幼苗的RNA群体中,出现较强的杂交条带,而在未诱导培养的甜菜幼苗的RNA群体中,则几乎没有阳性杂交条带出现。因此,Ty7Br600这一序列有可能是二年生甜菜幼苗经低温诱导处理之后被诱导表达的与抽薹相关的新基因序列。Southern杂交结果发现,在每一组酶切中至少有2~3条条带,表明Ty7Br600确为甜菜基因组片段,也同时表明该基因在甜菜基因组中以2个拷贝或低拷贝形式存在。

Comparative Experiments on Different Varieties of Phaseolus vulgaris L. under Dry Farming Conditions

[Objective] The aim was to screen varieties of Phaseolus vulgaris L.for cultivation in arid,semi-arid areas.[Method] The cultivation experiments of seven varieties of P.vulgaris under dry farming conditions were carried out in typical dry farming agriculture region Yangqu County in North China.[Result] The plants of Teyoutejiadou,Chaojiwujing and Xinxinyichiqingyuanlong were relatively strong;the growth rate of Aifeng,Jingxuanjiadouwang and Chaochangsijidou was relatively rapid;the transpiration rate of functional leaves of Aifeng was the lowest.The yield of Tianma 95-33 jiadouwang was significant higher than that of other varieties,the unit yield was up to 36.33 t/hm2,which was 1.39 times higher than that of Aifeng and 1.9 times higher than that of Xinxinyichiqingyuanlong;followed by the yield of Aifeng（26.07 t/hm2）,which was 1.37 times higher than that of Xinxinyichiqingyuanlong.[Conclusion] The yield of Tianma 95-33 jiadouwang and Aifeng was relatively high,as well as the protein content,indicating that they were suitable to be applied and cultivated in the experimental plot and surrounding areas.

L-肉碱对普通小球藻Chlorella vulgaris种群增长和生化组成的影响

分别以0,5,50 mg/L的L-肉碱(L-carnitine)处理淡水普通小球藻(Chlorella vulgaris),以SE为培养液,培养7 d,探究L-肉碱对淡水普通小球藻种群增长、总脂和可溶性蛋白质含量的影响。结果表明:不同质量浓度的L-肉碱在不同培养时期对普通小球藻种群增长影响显著(P<0.05);高浓度L-肉碱显著降低总脂含量(P<0.05),并使可溶性蛋白质含量显著升高(P<0.05)。结果提示,在培养液中添加外源L-肉碱可能通过直接作用于普通小球藻,或促进培养液中微小生物分泌活性物质间接影响普通小球藻的种群增长及总脂和可溶性蛋白质的含量。

Chemical Compositions and Antibacterial Activity of Essential Oil from Thymus Seedlings of Tissue Culture

[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. [Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry （GC/MS） and peak area normalization method; and the antimicrobial activity was tested by filter paper method. [Result] A total of 54 compositions were identified from these three samples, they had similar main compositions： thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. [Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.

紫冠豆角(Phaseolus vulgaris L.)种子中α-淀粉酶抑制剂的提取及其性质研究

本研究采用盐溶的方法从紫冠豆角(Phaseolus vulgaris L.)种子中提取α-淀粉酶抑制剂(α-AI),将提取的α-AI用不同温度和pH处理,并评价其稳定性。在此基础上,以提取物对猪胰腺α-淀粉酶的抑制活性(IC_(50))为指标,分析了豆粉的细度(A)、料液比(B)、盐溶时间(C)三个因素对α-AI提取效果的影响,利用响应面分析法优化提取条件,通过对老豆角种子的深加工,实现提高其附加值的目的。结果表明:该α-AI为耐热性蛋白,在pH4~10的范围性质稳定,影响α-AI的IC_(50)因素按主次顺序排列为:豆粉的细度>料液比>盐溶时间,确定提取α-AI最佳工艺条件为:豆粉过60目筛(粒径<0.3 mm),料液质量体积比为1∶12(g/mL)、盐溶时间7.75 h。紫冠豆角种子α-AI的IC_(50)最优值为27.036±0.235μg/mL。