Background： Vascular endothelial growth factor A （VEGFA） can induce endothelial cell proliferation, promote cell migration, and inhibit apoptosis. These processes play key roles in physiological blood vessel format...Background： Vascular endothelial growth factor A （VEGFA） can induce endothelial cell proliferation, promote cell migration, and inhibit apoptosis. These processes play key roles in physiological blood vessel formation and pathological angiogenesis. Methods： In this study, we examined VEGFA gene expression in the heart, liver, and kidney of Tibetan pigs （-I-P）, Yorkshire pigs that migrated to high altitudes （YH）, and Yorkshire pigs that lived at low altitudes （YL）. We used PCR and Sanger sequencing to screen for single nucleotide polymorphisms （SNPs） in 5＇-flanking DNA and exons of the VEGFA gene. Quantitative real-time PCR and western blots were used to measure expression levels and PCR products were sequenced. Results： Results showed that the VEGFA mRNA and protein expression in heart, liver and kidney of TP was higher than that in YH and YL. In addition, the mRNA sequence of the pig VEGFA gene was conserved among pig breeds, and only five SNPs were found in the 5＇-flanking region of the VEGFA gene, the allele frequency distributions of the 5 SNPs were not significantly different between the TP, Yorkshire （YL）, and Diannan small-ear （DN） pig populations. Conclusion： In conclusion, the Tibetan pig showed high tissues, which suggests that the VEGFA gene may play a levels of VEGFA gene expression in several hypoxic major functional role in hypoxic adaptation.展开更多
Background: Tibetan pigs, which inhabit the Tibetan Plateau, exhibit distinct phenotypic and physiological characteristics from those of lowland pigs and have adapted well to the extreme conditions at high altitude.Ho...Background: Tibetan pigs, which inhabit the Tibetan Plateau, exhibit distinct phenotypic and physiological characteristics from those of lowland pigs and have adapted well to the extreme conditions at high altitude.However, the genetic and epigenetic mechanisms of hypoxic adaptation in animals remain unclear.Methods: Whole-genome DNA methylation data were generated for heart tissues of Tibetan pigs grown in the highland(TH, n = 4) and lowland(TL, n = 4), as well as Yorkshire pigs grown in the highland(YH, n = 4) and lowland(YL, n = 4), using methylated DNA immunoprecipitation sequencing.Results: We obtained 480 million reads and detected 280679, 287224, 259066, and 332078 methylation enrichment peaks in TH, YH, TL, and YL, respectively. Pairwise TH vs. YH, TL vs. YL, TH vs. TL, and YH vs. YL comparisons revealed6829, 11997, 2828, and 1286 differentially methylated regions(DMRs), respectively. These DMRs contained 384, 619,192, and 92 differentially methylated genes(DMGs), respectively. DMGs that were enriched in the hypoxia-inducible factor 1 signaling pathway and pathways involved in cancer and hypoxia-related processes were considered to be important candidate genes for high-altitude adaptation in Tibetan pigs.Conclusions: This study elucidates the molecular and epigenetic mechanisms involved in hypoxic adaptation in pigs and may help further understand human hypoxia-related diseases.展开更多
[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig....[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig. [ Method ] In the study, real-time quantitative RT-PCR method was established to compare and analyze the differential expression of five porcine reproductive and respiratory syndrome virus （PRRSV） receptor genes （HSPG2, SIGLEC1, CD163, VIM and NMMHC-H A） in lung tissues in Tibetan pig, Zangmei pig and Yorkshire pig before the challenge and at the 4th ,7th and 14th days after the challenge with JXAI isolate. [ Results ] HSPG2 expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the challenge with JXAI （ P 〈 0.05 ）, while decreased significantly at the 7th day after the challenge （P 〈 0.05 ）, HSPG2 expression in Zangmei pig lung tissues increased significantly at the 14th day after the challenge （P〈0.05）. SIGLECl expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the infection（P 〈 0.05 ）, while SIGLEC 1 expression in Yorkshire pig decreased significantly at the 4th, 7th and 14th days after the challenge （P 〈0. 05 ）. CD163 expression in lung tissues of Tibetan pig and Zangmei pig both increased significantly at the 14th day after the challenge （P 〈 0.05 ）, while CD163 expression in lung tissues of Yorkshire pig decreased significantly at the 7th and 14th days after the challenge （ P 〈 0. 05 ）. VIM expression in lung tissues of Tibetan pig increased significantly at the 7th day after the challenge （ P 〈 0. 05 ）, while which of Yorkshire pig at the 7th day after the challenge decreased significantly （ P 〈 0. 05 ）. NMMHC-II A expression in lung tissues of Zangmei pig increased significantly at the 4th day after the challenge （ P 〈 0. 05 ）, and which of Yorkshire pig increased significantly at the 4th and 14th days after the challenge （P 〈 0. 05 ）. [ Conclusion] SIGLEC1 and VIM genes might be the potential key genes affecting the susceptibility of JXA1 isolate on Tibetan pig, Zangrnei pig and Yorkshire pig. Key words JXA1 isolate; Tibetan pig; Zangmei pig; Yorkshire pig; Porcine reproductive and respiratory syndrome virus receptor genes; Differential expression展开更多
[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转...[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转录组测序,测序数据经短时间序列表达模式(STEM)趋势分析、功能富集分析和互作网络分析。[结果]40 kg vs 80 kg、80 kg vs 120 kg和40 kg vs 120 kg分别筛选到1517、486和1752个显著差异基因;差异基因STEM分析显示:模块4和1的基因先显著下调后基本不变,模块15、12和11的基因随体质量增加而显著上调,模块0的基因随体质量增加而显著下调;WNT10B、CPT1B和C5AR2位于模块4和1的基因网络核心,PLA2G7、WWTR1、SPP1、SERPINE1和PTPN11位于模块15、12和11的基因网络核心,ADIPOQ、CH25H和IL10位于模块0的基因网络核心;WNT10B和CPT1B等11个基因的qPCR验证结果与转录组测序结果一致。[结论]WNT10B和PTPN11等11个核心基因以协作方式参与大型迪庆藏猪腹膜脂肪代谢调控,结果可为迪庆藏猪的遗传改良提供参考。展开更多
Linzhi Native Pig is an unique local breed recently discovered in the hinterland of Tibet. Its geological distribution, natural environment and ecological conditions have been explored. Using random sampling in typica...Linzhi Native Pig is an unique local breed recently discovered in the hinterland of Tibet. Its geological distribution, natural environment and ecological conditions have been explored. Using random sampling in typical colony of classification and standard animal-scientific and biogenetic techniques, we examined its contour features, size and weight, reproductive performances, carcass characters, meat quality, fresh-keeping features and the frequency distribution in the 19 structural gene loci encoding enzymes and proteins; according to folklores and Tibetan, Chinese and English history books, the materials and literature of Tibetan Studies, we have analyzed its origin; by visiting and requesting eminent Lamaists, practitioners of Tibetan medicine, farmers and herdsmen who self or relatives once treated with products from Linzhi Native Pigs, we recorded and summarized its application as a Tibetan medicinal material. Our findings make certain that Linzhi Native Pig holds great potential value in economy and culture.展开更多
基金supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2016ZX08009-003-006)the National Key Technology R&D Program (2012BAD03B03)the Program for Changjiang Scholar and Innovation Research Team in University (IRT1191)
文摘Background： Vascular endothelial growth factor A （VEGFA） can induce endothelial cell proliferation, promote cell migration, and inhibit apoptosis. These processes play key roles in physiological blood vessel formation and pathological angiogenesis. Methods： In this study, we examined VEGFA gene expression in the heart, liver, and kidney of Tibetan pigs （-I-P）, Yorkshire pigs that migrated to high altitudes （YH）, and Yorkshire pigs that lived at low altitudes （YL）. We used PCR and Sanger sequencing to screen for single nucleotide polymorphisms （SNPs） in 5＇-flanking DNA and exons of the VEGFA gene. Quantitative real-time PCR and western blots were used to measure expression levels and PCR products were sequenced. Results： Results showed that the VEGFA mRNA and protein expression in heart, liver and kidney of TP was higher than that in YH and YL. In addition, the mRNA sequence of the pig VEGFA gene was conserved among pig breeds, and only five SNPs were found in the 5＇-flanking region of the VEGFA gene, the allele frequency distributions of the 5 SNPs were not significantly different between the TP, Yorkshire （YL）, and Diannan small-ear （DN） pig populations. Conclusion： In conclusion, the Tibetan pig showed high tissues, which suggests that the VEGFA gene may play a levels of VEGFA gene expression in several hypoxic major functional role in hypoxic adaptation.
基金supported by the National Natural Science Foundation of China(No.31560615)the National Key Technology Research and Development Program(No.2015BAD03B02)+1 种基金the Program for Changjiang Scholars and Innovation Research Team in University(No.IRT_15R62)the Innovation Base Cultivation and Development Project(No.Z171100002217072)
文摘Background: Tibetan pigs, which inhabit the Tibetan Plateau, exhibit distinct phenotypic and physiological characteristics from those of lowland pigs and have adapted well to the extreme conditions at high altitude.However, the genetic and epigenetic mechanisms of hypoxic adaptation in animals remain unclear.Methods: Whole-genome DNA methylation data were generated for heart tissues of Tibetan pigs grown in the highland(TH, n = 4) and lowland(TL, n = 4), as well as Yorkshire pigs grown in the highland(YH, n = 4) and lowland(YL, n = 4), using methylated DNA immunoprecipitation sequencing.Results: We obtained 480 million reads and detected 280679, 287224, 259066, and 332078 methylation enrichment peaks in TH, YH, TL, and YL, respectively. Pairwise TH vs. YH, TL vs. YL, TH vs. TL, and YH vs. YL comparisons revealed6829, 11997, 2828, and 1286 differentially methylated regions(DMRs), respectively. These DMRs contained 384, 619,192, and 92 differentially methylated genes(DMGs), respectively. DMGs that were enriched in the hypoxia-inducible factor 1 signaling pathway and pathways involved in cancer and hypoxia-related processes were considered to be important candidate genes for high-altitude adaptation in Tibetan pigs.Conclusions: This study elucidates the molecular and epigenetic mechanisms involved in hypoxic adaptation in pigs and may help further understand human hypoxia-related diseases.
基金Supported by Sichuan Public Welfare Scientific Research Institutes Basic Research Projects(SASA2015A03)Sichuan Science and Technology Support Program(2014NZ009,16ZC2850)National Pig Industry Technology System(CARS-36)
文摘[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig. [ Method ] In the study, real-time quantitative RT-PCR method was established to compare and analyze the differential expression of five porcine reproductive and respiratory syndrome virus （PRRSV） receptor genes （HSPG2, SIGLEC1, CD163, VIM and NMMHC-H A） in lung tissues in Tibetan pig, Zangmei pig and Yorkshire pig before the challenge and at the 4th ,7th and 14th days after the challenge with JXAI isolate. [ Results ] HSPG2 expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the challenge with JXAI （ P 〈 0.05 ）, while decreased significantly at the 7th day after the challenge （P 〈 0.05 ）, HSPG2 expression in Zangmei pig lung tissues increased significantly at the 14th day after the challenge （P〈0.05）. SIGLECl expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the infection（P 〈 0.05 ）, while SIGLEC 1 expression in Yorkshire pig decreased significantly at the 4th, 7th and 14th days after the challenge （P 〈0. 05 ）. CD163 expression in lung tissues of Tibetan pig and Zangmei pig both increased significantly at the 14th day after the challenge （P 〈 0.05 ）, while CD163 expression in lung tissues of Yorkshire pig decreased significantly at the 7th and 14th days after the challenge （ P 〈 0. 05 ）. VIM expression in lung tissues of Tibetan pig increased significantly at the 7th day after the challenge （ P 〈 0. 05 ）, while which of Yorkshire pig at the 7th day after the challenge decreased significantly （ P 〈 0. 05 ）. NMMHC-II A expression in lung tissues of Zangmei pig increased significantly at the 4th day after the challenge （ P 〈 0. 05 ）, and which of Yorkshire pig increased significantly at the 4th and 14th days after the challenge （P 〈 0. 05 ）. [ Conclusion] SIGLEC1 and VIM genes might be the potential key genes affecting the susceptibility of JXA1 isolate on Tibetan pig, Zangrnei pig and Yorkshire pig. Key words JXA1 isolate; Tibetan pig; Zangmei pig; Yorkshire pig; Porcine reproductive and respiratory syndrome virus receptor genes; Differential expression
文摘[目的]筛选大型迪庆藏猪不同生长阶段腹脂代谢差异的功能基因并解析其调控网络。[方法]选择胎次相同、出生日期及体质量相近的大型迪庆藏猪36头,随机分为3组进行育肥试验,分别在体质量达40、80和120 kg屠宰,每组采集3头猪的腹脂进行转录组测序,测序数据经短时间序列表达模式(STEM)趋势分析、功能富集分析和互作网络分析。[结果]40 kg vs 80 kg、80 kg vs 120 kg和40 kg vs 120 kg分别筛选到1517、486和1752个显著差异基因;差异基因STEM分析显示:模块4和1的基因先显著下调后基本不变,模块15、12和11的基因随体质量增加而显著上调,模块0的基因随体质量增加而显著下调;WNT10B、CPT1B和C5AR2位于模块4和1的基因网络核心,PLA2G7、WWTR1、SPP1、SERPINE1和PTPN11位于模块15、12和11的基因网络核心,ADIPOQ、CH25H和IL10位于模块0的基因网络核心;WNT10B和CPT1B等11个基因的qPCR验证结果与转录组测序结果一致。[结论]WNT10B和PTPN11等11个核心基因以协作方式参与大型迪庆藏猪腹膜脂肪代谢调控,结果可为迪庆藏猪的遗传改良提供参考。
文摘Linzhi Native Pig is an unique local breed recently discovered in the hinterland of Tibet. Its geological distribution, natural environment and ecological conditions have been explored. Using random sampling in typical colony of classification and standard animal-scientific and biogenetic techniques, we examined its contour features, size and weight, reproductive performances, carcass characters, meat quality, fresh-keeping features and the frequency distribution in the 19 structural gene loci encoding enzymes and proteins; according to folklores and Tibetan, Chinese and English history books, the materials and literature of Tibetan Studies, we have analyzed its origin; by visiting and requesting eminent Lamaists, practitioners of Tibetan medicine, farmers and herdsmen who self or relatives once treated with products from Linzhi Native Pigs, we recorded and summarized its application as a Tibetan medicinal material. Our findings make certain that Linzhi Native Pig holds great potential value in economy and culture.
