The reports about tissue culture technology of Bletilla striata were summa- rized in this paper, and explants for tissue culture, induction differentiation of callus, rooting culture and transplanting, effect of growt...The reports about tissue culture technology of Bletilla striata were summa- rized in this paper, and explants for tissue culture, induction differentiation of callus, rooting culture and transplanting, effect of growth regulator and influence of external environment were expounded. This study provides support for further research.展开更多
The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS...The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.展开更多
Rhizoma Bletillae is in high demand as a traditional Chinese medicine, and the natural resources have been severely damaged due to excessive exploitation. Because Bletilla striata seeds are small and have no endosperm...Rhizoma Bletillae is in high demand as a traditional Chinese medicine, and the natural resources have been severely damaged due to excessive exploitation. Because Bletilla striata seeds are small and have no endosperm, the seed germination rate is low in natural conditions. Traditional division propagation could not meet the demands of large-scale cultivation. Tissue culture can provide many seedlings in a short time and is more effective and convenient than other methods. Most studies on tissue culture of B. striata selected seeds as explants. This review summarized the processes of the aseptic seed germination pathway. It included such stages as seed germination, proliferation of clusters of buds, induction of root- ing and transplanting of seedlings. Influential factors as well as optimum combination and concentration of the plant growth regulators of each stage were also summarized. The further research on tissue culture of B. striata was also prospected.展开更多
文摘The reports about tissue culture technology of Bletilla striata were summa- rized in this paper, and explants for tissue culture, induction differentiation of callus, rooting culture and transplanting, effect of growth regulator and influence of external environment were expounded. This study provides support for further research.
基金Supported by Students’S&T Innovation Project of Zhejiang Colleges and Universities(2015R463001)~~
文摘The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.
基金supported by grants from the National Natural Science Foundation of China (No.81673552)CAMS Innovation Fund for Medical Sciences (CIFMS,2016-I2M-3-016)
文摘Rhizoma Bletillae is in high demand as a traditional Chinese medicine, and the natural resources have been severely damaged due to excessive exploitation. Because Bletilla striata seeds are small and have no endosperm, the seed germination rate is low in natural conditions. Traditional division propagation could not meet the demands of large-scale cultivation. Tissue culture can provide many seedlings in a short time and is more effective and convenient than other methods. Most studies on tissue culture of B. striata selected seeds as explants. This review summarized the processes of the aseptic seed germination pathway. It included such stages as seed germination, proliferation of clusters of buds, induction of root- ing and transplanting of seedlings. Influential factors as well as optimum combination and concentration of the plant growth regulators of each stage were also summarized. The further research on tissue culture of B. striata was also prospected.
