12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight...12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.展开更多
This study aimed to investigate the effects of Radix Curcumae fungicides on tobacco brown spot and yield of flue-cured tobacco leaves. Through field experi- ments, control efficiency of Radix Curcumae fungicides again...This study aimed to investigate the effects of Radix Curcumae fungicides on tobacco brown spot and yield of flue-cured tobacco leaves. Through field experi- ments, control efficiency of Radix Curcumae fungicides against tobacco brown spot was investigated. The results showed that the average control efficiency of 400-fold, 600-fold and 800-fold Radix Curcumae fungicides against tobacco brown spot reached 37.83%, 34.09% and 24.32%, respectively. This study could provide the ba- sis for screening and application of plant-derived fungicides against tobacco brown spot.展开更多
[Objective] The paper was to understand the suitable meteorological indexes and key meteorological factors for infection and prevalence of tobacco brown spot. [ Method] Tobacco brown spot disease in five topical mount...[Objective] The paper was to understand the suitable meteorological indexes and key meteorological factors for infection and prevalence of tobacco brown spot. [ Method] Tobacco brown spot disease in five topical mountainous climatic areas of Guizhou Province was investigated, and the difference of biological characteristics of different strains was analyzed indoor. Different meteorological conditions （light, temperature and humidity） were simulated in artificial climate box by using the uniform design method, and the difference of infection ability of Alternaria alternate in different climate zones was analyzed in leaves in vitro by hanging drop method. [ Result] The infection rate of A. alternate in different climate zones of Guizhou Province showed certain difference. In low temperature and mid- die humidity （the humidity is 50% -60%, the temperature is 10 -20 ℃） conditions, the pathogen could infect tobacco leaves, various strains had certain difference, but the difference was small. [ Conclusion] The paper provided theoretical basis for prevalence prediction and control of tobacco brown spot.展开更多
[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of t...[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.展开更多
基金Supported by China National Tobacco Corporation[No.110202101048(LS-08)]Hundred’Level Innovative Talent Foundation of Guizhou Province(No.GCC[2022]028-1,GCC[2023]108)+2 种基金Guizhou Science Technology Foundation(No.ZK[2021]Key036)the National Natural Science Foundation of China(No.32160522)Guizhou Province Applied Technology Research and Development Funding Post-subsidy Project and Guizhou Tobacco Company(No.2020XM03,2020XM22,2024XM06).
文摘12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC_(50) value of 0.51μg/mL),conidia germination(average EC_(50) value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.
基金Supported by Independent Project of Luzhou Branch of Sichuan Tobacco Company(20140105)
文摘This study aimed to investigate the effects of Radix Curcumae fungicides on tobacco brown spot and yield of flue-cured tobacco leaves. Through field experi- ments, control efficiency of Radix Curcumae fungicides against tobacco brown spot was investigated. The results showed that the average control efficiency of 400-fold, 600-fold and 800-fold Radix Curcumae fungicides against tobacco brown spot reached 37.83%, 34.09% and 24.32%, respectively. This study could provide the ba- sis for screening and application of plant-derived fungicides against tobacco brown spot.
基金Supported by Major Special Project of Guizhou Branch([2011] No.6003)National Natural Science Foundation of China (41040039)
文摘[Objective] The paper was to understand the suitable meteorological indexes and key meteorological factors for infection and prevalence of tobacco brown spot. [ Method] Tobacco brown spot disease in five topical mountainous climatic areas of Guizhou Province was investigated, and the difference of biological characteristics of different strains was analyzed indoor. Different meteorological conditions （light, temperature and humidity） were simulated in artificial climate box by using the uniform design method, and the difference of infection ability of Alternaria alternate in different climate zones was analyzed in leaves in vitro by hanging drop method. [ Result] The infection rate of A. alternate in different climate zones of Guizhou Province showed certain difference. In low temperature and mid- die humidity （the humidity is 50% -60%, the temperature is 10 -20 ℃） conditions, the pathogen could infect tobacco leaves, various strains had certain difference, but the difference was small. [ Conclusion] The paper provided theoretical basis for prevalence prediction and control of tobacco brown spot.
基金Supported by State Tobacco Monopoly Administration Project "National Survey of Pests in Tobacco" (110200902065)Yunnan Tobacco Monopoly Bureau Technology Project " Investigation of Tobacco Pests in Yunnan Province" (2010YN19)~~
文摘[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.
