Nitrogen(N)supply is the most important factor affecting yield and quality of flue-cured tobacco(FCT).A field experiment and an in situ incubation method were used to study the effects of soil N mineralization in the ...Nitrogen(N)supply is the most important factor affecting yield and quality of flue-cured tobacco(FCT).A field experiment and an in situ incubation method were used to study the effects of soil N mineralization in the later stages of growth on yield and nicotine content of FCT in Fenggang and Jinsha,Guizhou Province.The yield and market value of FCT at Fenggang were much lower than those at Jinsha.However,the nicotine content of middle and upper leaves was much higher at Fenggang than at Jinsha when the same rate of fertilizer N was applied,which might be due to a higher N supply capacity at the Fenggang site.At later stages of growth(7-16 weeks after transplanting),the soil net N mineralization at Fenggang(56 kg N ha^(-1))was almost double that at Jinsha(30 kg N ha^(-1)).While soil NH_4-N and NO_3-N were almost exhausted by the plants or leached 5 weeks after transplanting,the N taken up at the later growth stages at Fenggang were mainly derived from soil N mineralization,which contributed to a high nicotine content in the upper leaves.The order of soil N contribution to N buildup in different leaves was:upper leaves>middle leaves>lower leaves.Thus,soil N mineralization at late growth stages was an important factor affecting N accumulation and therefore the nicotine content in the upper leaves.展开更多
[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of t...[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.展开更多
The effect of different ratio of organic and inorganic fertilizers on the growth and the yield and quality of flue-cured tobacco was studied.Using randomized blocks design,the data of the test materials K326 were anal...The effect of different ratio of organic and inorganic fertilizers on the growth and the yield and quality of flue-cured tobacco was studied.Using randomized blocks design,the data of the test materials K326 were analyzed by using variance analysis,significance test and other methods.The release efficiency of organic fertilizer in the soil was slower,and the effect of organic fertilizer on the growth of tobacco plants was worse than that of chemical fertilizers,and under 25% organic fertilizer + 75% chemical fertilizer treatment,the quality of the fluecured tobacco was good.Under the treatment of 50% organic fertilizer + 50% chemical fertilizer,the production of flue-cured tobacco was high and the ratio of first-class tobacco was high.It is suggested that the organic fertilizer application ratio should be 25%-50% in the agricultural production.展开更多
The effects of amount of green manure returned to field on yield and quality of flue-cured tobacco were studied by field experiment. The results showed that significant positive correlation existed between tobacco lea...The effects of amount of green manure returned to field on yield and quality of flue-cured tobacco were studied by field experiment. The results showed that significant positive correlation existed between tobacco leaf yield and small or moderate amount of green manure returned to field. Path analysis showed that moderate amount of green manure returned to field affected yield and quality of tobacco leaves, small green manure returned to field mainly affected leaf yield, while large amount of green manure returned to field mainly affected leaf quality. Therefore, the effect of moderate amount of green manure returned to field on yield and quality of tobacco leaves was best, and moderate amount of green manure returned to field was recommended in production of flue-cured tobacco.展开更多
The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Ni...The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.展开更多
基金the Program for Changjiang Scholars and Innovation Research Team in University(No.IRT0511)
文摘Nitrogen(N)supply is the most important factor affecting yield and quality of flue-cured tobacco(FCT).A field experiment and an in situ incubation method were used to study the effects of soil N mineralization in the later stages of growth on yield and nicotine content of FCT in Fenggang and Jinsha,Guizhou Province.The yield and market value of FCT at Fenggang were much lower than those at Jinsha.However,the nicotine content of middle and upper leaves was much higher at Fenggang than at Jinsha when the same rate of fertilizer N was applied,which might be due to a higher N supply capacity at the Fenggang site.At later stages of growth(7-16 weeks after transplanting),the soil net N mineralization at Fenggang(56 kg N ha^(-1))was almost double that at Jinsha(30 kg N ha^(-1)).While soil NH_4-N and NO_3-N were almost exhausted by the plants or leached 5 weeks after transplanting,the N taken up at the later growth stages at Fenggang were mainly derived from soil N mineralization,which contributed to a high nicotine content in the upper leaves.The order of soil N contribution to N buildup in different leaves was:upper leaves>middle leaves>lower leaves.Thus,soil N mineralization at late growth stages was an important factor affecting N accumulation and therefore the nicotine content in the upper leaves.
基金Supported by State Tobacco Monopoly Administration Project "National Survey of Pests in Tobacco" (110200902065)Yunnan Tobacco Monopoly Bureau Technology Project " Investigation of Tobacco Pests in Yunnan Province" (2010YN19)~~
文摘[Objective] The paper was to study the effect of tobacco blown spot on the yield and output value of tobacco leaf.[Method]The upper,middle and lower leaves in tobacco plant were selected during the harvest period of tobacco to carry out loss rate estimation of yield and output value of tobacco leaf caused by different disease levels of brown spot.Regression correlation analysis was also conducted.[Result]The disease levels of brown spot had extremely significant strong negative correlation with single leaf weight of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of single leaf weight.The increase speed of loss rate of single leaf weight of middle and upper leaves was obviously faster than that of lower leaves.The loss rates of single leaf weight of upper,middle and lower leaves were 3.18%-28.95%,3.43%-28.88% and 10.07%-26.90%,respectively.The higher the disease level of blown spot was,the lower the yield and output value of tobacco leaf was,and the corresponding loss rate was also higher.Correlation analysis showed that the disease level of blown spot had extremely significant strong negative correlation with the yield and output value of tobacco leaf,and it had extremely significant strong positive correlation with the loss rate of yield and output value.The negative impact of blown spot on the output value of tobacco leaf was far greater than that on the yield.The highest loss rate of the yield of tobacco leaf was 28.56%,while the highest loss rate of output value reached 89.67%.[Conclusion] The study provided theoretical basis for accurately holding the critical period for the control of blown spot,thus reducing the damage on tobacco leaf and improving the output value of tobacco leaf.
基金Supported by Yunnan Tobacco Company Project(2015YN19,2014YN25)
文摘The effect of different ratio of organic and inorganic fertilizers on the growth and the yield and quality of flue-cured tobacco was studied.Using randomized blocks design,the data of the test materials K326 were analyzed by using variance analysis,significance test and other methods.The release efficiency of organic fertilizer in the soil was slower,and the effect of organic fertilizer on the growth of tobacco plants was worse than that of chemical fertilizers,and under 25% organic fertilizer + 75% chemical fertilizer treatment,the quality of the fluecured tobacco was good.Under the treatment of 50% organic fertilizer + 50% chemical fertilizer,the production of flue-cured tobacco was high and the ratio of first-class tobacco was high.It is suggested that the organic fertilizer application ratio should be 25%-50% in the agricultural production.
基金Supported by Project of Hunan Tobacco Monopoly Bureau
文摘The effects of amount of green manure returned to field on yield and quality of flue-cured tobacco were studied by field experiment. The results showed that significant positive correlation existed between tobacco leaf yield and small or moderate amount of green manure returned to field. Path analysis showed that moderate amount of green manure returned to field affected yield and quality of tobacco leaves, small green manure returned to field mainly affected leaf yield, while large amount of green manure returned to field mainly affected leaf quality. Therefore, the effect of moderate amount of green manure returned to field on yield and quality of tobacco leaves was best, and moderate amount of green manure returned to field was recommended in production of flue-cured tobacco.
文摘The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.
