期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury 被引量:1
1
作者 Guangyi Liu Li Zhao +2 位作者 Tingting Wang Meizeng Zhang Haitao Pei 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第15期1437-1445,共9页
A preliminary study from our research group showed that picroside II inhibited neuronal apop- tosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. ... A preliminary study from our research group showed that picroside II inhibited neuronal apop- tosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside Ⅱ inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5-2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit. 展开更多
关键词 nerve regeneration picroside II therapeutic dose time window brain ischemia neuron-specific enolase toluidine blue staining flow cytometry immunohistochemical assay western blot RT-PCR rats NSFC grant neural regeneration
下载PDF
CARTILAGE- LIKE PHENOMENON IN THE ANTERIOR CRUCIATE LIGAMENT 被引量:1
2
作者 蒋青 林共周 +2 位作者 曲绵域 崔国庆 滕华建 《Chinese Medical Sciences Journal》 CAS CSCD 2001年第2期103-106,共4页
Objective. To detect histological characteristic of anterior cruciate ligament (ACL) and medial collateral ligament (MCL). Methods. In each of 20 skeletally mature male mongrels and 4 men, the ACL and MCL were examine... Objective. To detect histological characteristic of anterior cruciate ligament (ACL) and medial collateral ligament (MCL). Methods. In each of 20 skeletally mature male mongrels and 4 men, the ACL and MCL were examined by standard hematoxylin- eosin procedure and toluidine blue staining for histologic observation. Results. The fibroblasts in medial collateral are elongated to spindle shape and aligned in a row between the bundles of collagenous fibers. Toluidine blue staining is negative. The anterior cruciate ligament demonstrated more heterogenous cell types and arrangement. It had three major cell forms:spindle, round and ovoid type, which were shorter but greater than the cells in medial collateral ligament. Toluidine blue staining was positive in anterior cruciate ligament. Most cells in anterior cruciate ligament were enclosed within lacunae. Conclusion. This study suggests that the ACL has different histological characteristics from MCL, and is more cartilage- like in nature. 展开更多
关键词 anterior cruciate ligament toluidine blue staining CARTILAGE
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部