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Studies on the Isolation of Endophytic Bacteria from Tomato Plants and Their Growth-promoting Activities 被引量:10
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作者 张立新 宋江华 刘慧平 《Agricultural Science & Technology》 CAS 2008年第5期1-3,69,共4页
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for t... [Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity. 展开更多
关键词 tomato ENDOPHYTIC BACTERIA ISOLATION growth-PROMOTING activity
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Effect of γ-PGA Coated Urea on N-Release Rate and Tomato Growth 被引量:2
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作者 JIANG Yue LIU Liming +3 位作者 LUO Guangfu ZHANG Zhanli Davidmark J HUANG Yingping 《Wuhan University Journal of Natural Sciences》 CAS 2014年第4期335-340,共6页
The anionic polymer γ-polyglutamic acid (γ-PGA) was used to coat urea and slow its dissolution. Three types of slow release urea (SRU) fertilizers (polymer coated urea with pore constriction, polymer coated ure... The anionic polymer γ-polyglutamic acid (γ-PGA) was used to coat urea and slow its dissolution. Three types of slow release urea (SRU) fertilizers (polymer coated urea with pore constriction, polymer coated urea with enzyme inhibitor and polymer coated urea with pore constriction and enzyme inhibitor) were prepared and tested for the N-release rate. After using SRU, the effect on the tomato growth was analyzed. The extracts of SRU were analyzed for NH3/NH4+-N and NO3--N. The N-release rate was used to determine the optimum ratio of ingredients. The results show that the three types of SRU met the dissolution rate standards recommended by the Com- mittee of European Normalization. γ-PGA SRU increased the chlorophyll content of tomato (flowering stage) by an average of 100% compared with that grew in untreated urea. The results from soil analysis (0-60 cm in tomato pots) indicate that the content of NH3/NH4+-N in SRU-treated pots was 25%-61% higher than that in soil from urea-treated pots during the growing period, while the content of NO3--N was nearly 50% lower after the tomato had been harvested. Newly formulated SRU fertilizer increases nitrogen up- take and reduces loss of applied nitrogen. Plant growth is enhanced, a valu- able resource is conserved, and the aquatic environment benefits from de- creased level of nitrate in agricultural run-off. 展开更多
关键词 Γ-PGA slow release urea (SRU) fertilizer nitrogen releaserate tomato growth
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Adjusted Nutrition Solution Formula Improves the Growth and Yield of Soilless Tomato Culture in Southern Xinjiang 被引量:1
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作者 Chunling WANG Deshuai WANG 《Agricultural Biotechnology》 CAS 2022年第6期158-162,166,共6页
The tomato production facilities in southern Xinjiang have unique climatic conditions.However,due to the high salinity and degree of mineralization of the soil and the extensive management of irrigation water sources,... The tomato production facilities in southern Xinjiang have unique climatic conditions.However,due to the high salinity and degree of mineralization of the soil and the extensive management of irrigation water sources,the tomato production efficiency is low,and there is still much room for improvement.In this study,the formulas of tomato nutrient solutions were adjusted according to the local irrigation water quality characteristics,and tomato was grown in a greenhouse using sand cultivation.To select a nutrient solution formula suitable for the tomato cultivated in the local facilities,various parameters of tomato treated with different formulas were compared.The results showed the following:①Adjusting the nutrient solution formula could effectively improve the vegetative and reproductive growth indexes of tomato.②Properly decreasing the nutrient solution concentration could improve the growth indexes of tomato.③Decreases in Ca^(2+) and Mg^(2+) in the nutrient solution did not affect the growth and yield of tomato.The possible reason for the third result was that excessive cations in the water inhibited the absorption of Ca^(2+) and Mg^(2+) in the nutrient solution and had antagonistic effects.Therefore,a high nutrient solution concentration does not necessarily promote the growth of tomatoes.In summary,this study will improve the production conditions of protected tomato in the southern Xinjiang area by supporting tomato nutrient solution adjustments according to actual conditions in combination with reasonable irrigation systems and scientific management. 展开更多
关键词 Nutrition Solution tomato growth Yield Southern Xinjiang
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Comparison of the Effect of Vermicompost and Inorganic Fertilizers on Vegetative Growth and Fruit Production of Tomato (<i>Solanum lycopersicum</i>L.) 被引量:6
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作者 Md. Abul Kashem Ashoka Sarker +1 位作者 Imam Hossain Md. Shoffikul Islam 《Open Journal of Soil Science》 2015年第2期53-58,共6页
This study attempted to compare the effect of cow manure vermicompost and inorganic fertilizers on the vegetative growth and fruits of tomato plant (Solanum lycopersicum L.). An air dried sandy loam soil was mixed wit... This study attempted to compare the effect of cow manure vermicompost and inorganic fertilizers on the vegetative growth and fruits of tomato plant (Solanum lycopersicum L.). An air dried sandy loam soil was mixed with five rates of vermicompost equivalent to 0 (control), 5, 10, 15 and 20 t ha-1 and three rates of NPK fertilizer equivalent to 50% (N-P-K = 69-16-35 kg ha-1), 100% (N-P-K = 137-32-70 kg ha-1) and 200% (N-P-K = 274-64-140 kg ha-1). The treatments were replicated three times. The data revealed that shoot length, number of leaves, dry matter weight of shoots and roots, fruit number and fruit weight were influenced significantly (P < 0.05) by the application of vermicompost and NPK fertilizer in the growth media. The highest dose of vermicompost of 20 t ha-1 increased dry weight of shoot of 52 folds and root of 115 folds, number of fruit(s)/plant of 6 folds and mean fruit weight of 29 folds while the highest rate of NPK fertilizer of 200% increased dry weight of shoot of 35 folds and root of 80 folds, number of fruit(s)/plant of 4 folds and mean fruit weight of 18 folds over the control treatment. The growth performance of tomato was better in the vermicompost amended soil pots than the plants grown in the inorganic fertilizer amended soil pots. This study suggested that the vermicompost served as a potential source of nutrients for plant growth. 展开更多
关键词 VERMICOMPOST NPK FERTILIZER tomato growth Yield
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Effect of cadmium on growth and photosynthesis of tomato seedlings 被引量:5
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作者 董静 邬飞波 张国平 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第10期974-980,共7页
A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 μmol/L Cd induced a slight increase in plant height of He... A hydroponic experiment carried out to study the effect of five Cd levels on growth and photosynthesis of two tomato cultivars showed that the addition of 0.1 μmol/L Cd induced a slight increase in plant height of Hezuo 903 and the SPAD (the Soil–Plant Analyses Development) value of the 2 cultivars. However, at higher Cd levels, i.e., 1 and 10 μmol/L, root length and volume, plant height, and SPAD value were all significantly reduced. On an average of the 2 cultivars, exposure to 1 and 10 μmol/L Cd for 33 d reduced plant height by 18.9% and 46.4% and SPAD value by 11.2% and 31.6%, compared with control, respectively. Similarly, root length was reduced by 41.1% and 25.8% and root volume by 45.2% and 63.7%, respectively. The addition of Cd in the growth medium also had significant deleterious effect on net photosynthetic rate (Pn) and intracellular CO2 concentration (Ci), with Pn being reduced by 27.2% and 62.1% at 1 μmol/L and 10 μmol/L Cd treatments compared to the control, respectively, while Ci increased correspondingly by 28.4% and 39.3%. 展开更多
关键词 Cadmium growth Photosynthesis tomato
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Effect of cold plasma treatment on seedling growth and nutrient absorption of tomato 被引量:2
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作者 蒋佳峰 李建刚 董元华 《Plasma Science and Technology》 SCIE EI CAS CSCD 2018年第4期47-50,共4页
The effects of cold plasma(CP) treatment on seed germination, seedling growth, root morphology, and nutrient uptake of a tomato were investigated. The results showed that 80 W of CP treatment significantly increased t... The effects of cold plasma(CP) treatment on seed germination, seedling growth, root morphology, and nutrient uptake of a tomato were investigated. The results showed that 80 W of CP treatment significantly increased tomato nitrogen(N) and phosphorus(P) absorption by12.7% and 19.1%, respectively. CP treatment significantly improved the germination potential of tomato seed by 11.1% and the germination rate by 13.8%. Seedling growth characteristics,including total dry weight, root dry weight, root shoot rate, and leaf area, significantly increased after 80 W of CP treatment. Root activity was increased by 15.7% with 80 W of CP treatment,and 12.6% with 100 W of CP treatment. CP treatment(80 W) markedly ameliorated tomato root morphology, and root length, surface area, and volume, which increased 21.3%, 23.6%, and29.0%, respectively. Our results suggested that CP treatment improved tomato N and P absorption by promoting the accumulation of shoot and root biomass, increasing the leaf area and root activity, and improving the length, surface area, and volume of root growth. Thus, CP treatment could be used in an ameliorative way to improve tomato nutrient absorption. 展开更多
关键词 cold plasma tomato nutrient uptake seedling growth root morphology
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Effect of Different Spacing and Urea Application Rates on Fruit Nutrient Composition, Growth and Yield of Tomato in Derived Savannah Vegetation of Kogi State, Nigeria 被引量:1
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作者 S. K. Ogundare J. A. Oloniruha +1 位作者 F. G. Ayodele I. A. Bello 《American Journal of Plant Sciences》 2015年第14期2227-2233,共7页
Experiments were located at Lower Niger River Basin Development Authority, Ejiba and Horticultural Section College of Agriculture, Kabba in 2014 cropping season to investigate the effect of different spacing and urea ... Experiments were located at Lower Niger River Basin Development Authority, Ejiba and Horticultural Section College of Agriculture, Kabba in 2014 cropping season to investigate the effect of different spacing and urea application rates on fruit nutrient composition, growth and fruit yield of tomato in derived savanna vegetation of Nigeria. Field experiments were arranged in a randomized complete block design in factorial fashion with three replications. The row spacing were 75 × 40 cm (33,333 plant·ha-1, 75 × 50 cm (26,666 plant·ha-1) and 75 × 60 cm (22,222 plant·ha-1) while the urea rates comprised control (0 kg urea·ha-1), 54.3 and 108.6 kg urea·ha-1. Growth and yield parameters taken were as follows: average plant height, stem girth, leaf area (m2) and yield per land area. Data were collected from ten randomly selected plants in each plot. The data were statistically analyzed using GENSTAT. The analysis of variance (ANOVA) was performed to find out the significance of variation among the treatments while the significant difference between mean treatments was separated using least significance Difference at 5% level of probability. The result obtained from this study indicated that urea application and spacing affected significantly growth parameters of tomato and yield per land area. It could be concluded that there was a significant increase in plant height, number of leaves, number of fruits per plant and final fruit weight of tomato as a result of urea fertilizer application at the rate of 108.6 kg urea/ha. However, this was not significantly better than plots with urea application at 54.8 kg/ha in both Ejiba and Kabba. Row spacing of 75 × 50 cm showed better performance in number of fruit and fruit yield per plots. Farmers in Ejiba and Kabba should apply urea at the rate of 54.8 kg per hectare and plant the crop at a row spacing of 75 × 50 cm for optimum yield and for a more profitable production of tomato. 展开更多
关键词 ROW SPACING tomato UREA Application growth Yield
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Effects of elevated CO_2 concentration on growth and water usage of tomato seedlings under different ammonium/nitrate ratios 被引量:8
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作者 LI Juan ZHOU Jian-min DUAN Zeng-qiang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2007年第9期1100-1107,共8页
Increasing atmospheric CO2 concentration is generally expected to enhance photosynthesis and growth of agricultural C3 vegetable crops, and therefore results in an increase in crop yield. However, little is known abou... Increasing atmospheric CO2 concentration is generally expected to enhance photosynthesis and growth of agricultural C3 vegetable crops, and therefore results in an increase in crop yield. However, little is known about the combined effect of elevated CO2 and N species on plant growth and development. Two growth-chamber experiments were conducted to determine the effects of NH4^+/NO3^- ratio and elevated CO2 concentration on the physiological development and water use of tomato seedlings. Tomato was grown for 45 d in containers with nutrient solutions varying in NH4^+/NO3^- ratios and CO2 concentrations in growth chambers. Results showed that plant height, stem thickness, total dry weight, dry weight of the leaves, stems and roots, G value (total plant dry weight/seedling days), chlorophyll content, photosynthetic rate, leaf-level and whole plant-level water use efficiency and cumulative water consumption of tomato seedlings were increased with increasing proportion of NO3- in nutrient solutions in the elevated CO2 treatment. Plant biomass, plant height, stem thickness and photosynthetic rate were 67%, 22%, 24% and 55% higher at elevated CO2 concentration than at ambient CO2 concentration, depending on the values of NH4^+/NO3^- ratio. These results indicated that elevating CO2 concentration did not mitigate the adverse effects of 100% NH4^+-N (in nutrient solution) on the tomato seedlings. At both CO2 levels, NH4^+/NO3^- ratios of nutrient solutions strongly influenced almost every measure of plant performance, and nitrate-fed plants attained a greater biomass production, as compared to ammonium-fed plants. These phenomena seem to be related to the coordinated regulation of photosynthetic rate and cumulative water consumption of tomato seedlings. 展开更多
关键词 CO2 enrichment NH4^+/NO3^- ratio physiological and biochemical indices growth water use tomato seedling
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Resistance of Different Tomato Varieties against Tomato Powdery Mildew during Different Growth Stages
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作者 Dong Huafang 《Plant Diseases and Pests》 CAS 2013年第2期15-17,44,共4页
The pathogen of tomato powdery mildew (Oidium neolycopersici Kiss) was simultaneously inoculated into four varieties during seedling, flowering and fruiting stages under the same environmental conditions by inducing... The pathogen of tomato powdery mildew (Oidium neolycopersici Kiss) was simultaneously inoculated into four varieties during seedling, flowering and fruiting stages under the same environmental conditions by inducing inoculation method, so as to study the resistance during different growth stages. Different varieties of plants and the plants during different growth stages were investigated after inoculation for 12 d, and disease epidemic curves were drawn according to survey data. The results showed that different varieties performed different resistance against powdery mildew and the same variety also showed different resistance during different growth stages. The susceptible extent of Lujia was the heaviest during seedling and flowering sages, while it showed the strongest resistance during fruiting stage; improved 96 -8 showed the highest resistance during seedling stage, but performed the lowest resistance during fruiting stage. 展开更多
关键词 tomato Different growth stages Powdery mildew DISEASE Epidemic curve
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Growth and Physiological Attributes of Tomato (Lycopersicon esculentum Mill.) Genotypes as Affected by NaCl Stress
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作者 M. Nasir Khan 《American Journal of Plant Sciences》 2016年第3期453-460,共8页
High level of salinity adversely affects growth, productivity as well as quality of vegetable crops by reducing photosynthetic capacity, enzyme activities, and by enhanced production of reactive oxygen species (ROS). ... High level of salinity adversely affects growth, productivity as well as quality of vegetable crops by reducing photosynthetic capacity, enzyme activities, and by enhanced production of reactive oxygen species (ROS). These ROS cause peroxidation of membrane lipids leading to disintegration of cell membrane with the leakage of electrolytes. All these detrimental effects ultimately contribute to the crop with reduced yield and low nutritive value. The present investigation was carried out to test salt tolerance capacity of ten genotypes of tomato on the basis of growth, physiological and biochemical characteristics. The results show that all the ten tested genotypes of tomato responded differently to 200 mM NaCl stress. Regarding growth parameters, BL-1076 gave higher values, while Queen gave the lowest values for most of the parameters (plant height, number of leaves, leaf area, shoot and root fresh weight and dry weight). Regarding physiological and biochemical parameters, BL-1076 gave higher values for the activities of carbonic anhydrase and nitrate reductase, leaf chlorophyll content, relative water content, and activities of antioxidant enzymes (superoxide dismutase, peroxidase and catalase). However, genotype Queen gave the lowest values for these parameters. On contrary, the least membrane damage (TBARS content) was registered in BL-1076, while the highest in Queen. Overall assessment of results leads to the conclusion that the genotype BL-1076 was found salt tolerant while Queen salt sensitive. The ten genotypes may be arranged on the basis of their salt tolerance capacity in decreasing order as: BL-1076 > Trust > Imperial > Tanshet star > PakmoreVF > L 26 > Plitz > Bonus F1 > Grace > Queen. 展开更多
关键词 Antioxidant Enzymes GENOTYPES growth STRESS tomato
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化肥与微生物菌肥配施对番茄生长、产量和品质的影响
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作者 姜宇 王娟 +5 位作者 刘力勇 谭巍 王志伟 冯一新 王琳 李金龙 《中国蔬菜》 北大核心 2024年第8期103-108,共6页
为探究化肥与微生物菌肥配合施用对番茄生长及果实品质的影响,以光辉101番茄为试材,设置7个不同施肥处理,即不施肥(CK)、配方施肥(M-1)、常规施肥(M-2)、化肥配施微生物菌剂(M-3),以及M-1、M-2、M-3分别减量1/3且基肥配施生物有机肥(M-4... 为探究化肥与微生物菌肥配合施用对番茄生长及果实品质的影响,以光辉101番茄为试材,设置7个不同施肥处理,即不施肥(CK)、配方施肥(M-1)、常规施肥(M-2)、化肥配施微生物菌剂(M-3),以及M-1、M-2、M-3分别减量1/3且基肥配施生物有机肥(M-4、M-5、M-6),研究不同施肥处理对番茄生长、产量及品质的影响。结果表明:M-3处理的番茄植株长势最好,与CK相比,株高、茎粗、节间数分别增加了19.44%、13.57%、15.15%,差异均达显著水平;单果质量、平均坐果数及单株产量、小区产量、总产量分别增加了3.45%、14.66%、19.35%、18.77%、18.77%。综合来看,每667 m2基肥施用硫酸钾、稳定性氮源掺混肥料50 kg及微生物菌剂(短小芽孢杆菌M101、哈茨木霉TH7、淡紫紫孢菌PL22)2 kg,追肥施用大量元素水溶肥(N∶P∶K=19∶19∶19)45 kg、大量元素水溶肥(N∶P∶K=15∶5∶30)15 kg及微生物菌剂24 kg,即化肥配施微生物菌剂(M-3)处理可作为番茄的最佳施肥方案。 展开更多
关键词 番茄 施肥处理 生长 产量 品质
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螯合钙叶面肥及遮阳网对番茄果实生长的影响
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作者 彭佃亮 王兴翠 +3 位作者 杨文霞 张敬敏 唐玉海 徐海成 《中国瓜菜》 CAS 北大核心 2024年第10期82-87,共6页
为探究叶面喷施螯合钙+遮阳网对温室春延茬番茄果皮代谢特征、果实裂果和品质的影响,以优品88番茄品种为材料,分别进行了CK(未遮阳+叶面喷施清水)、CC(未遮阳+叶面喷施0.30 g·L^(-1)螯合钙)、S(遮阳网+清水)与SCC(遮阳网+0.30 g... 为探究叶面喷施螯合钙+遮阳网对温室春延茬番茄果皮代谢特征、果实裂果和品质的影响,以优品88番茄品种为材料,分别进行了CK(未遮阳+叶面喷施清水)、CC(未遮阳+叶面喷施0.30 g·L^(-1)螯合钙)、S(遮阳网+清水)与SCC(遮阳网+0.30 g·L^(-1)螯合钙)的处理试验。结果表明,转色期与红熟期番茄果实裂果率均表现为SCC<S<CC<CK,且各处理间差异显著;与CK相比,CC、SCC处理均显著提高了果皮超氧化物歧化酶与抗坏血酸过氧化酶活性,降低了果皮丙二醛和H2O2含量;CC、S与SCC处理均显著降低了果皮细胞壁水解酶活性;果皮纤维素与原果胶含量呈现SCC>S>CC>CK的变化趋势,且SCC处理与其他各处理呈显著差异;CC与SCC处理均显著提高了果实单果质量、维生素C和可溶性固形物含量,且SCC处理效果最好。综上所述,在温室番茄生产中,0.30 g·L^(-1)螯合钙+遮阳网协同作用的增产提质效果最优。 展开更多
关键词 番茄 螯合钙 遮阳 果实生长
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不同植物生长调节剂对浙南番茄穴盘育苗的影响
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作者 苏世闻 付存念 +3 位作者 史建磊 王克磊 陈先知 宰文珊 《浙江农业科学》 2024年第5期1031-1036,共6页
为筛选出适宜浙南地区番茄壮苗培育的植物生长调节剂,以不同浓度的YELVSU P2-37、多效唑、矮壮素、壮苗1号处理番茄砧木品种浙砧7号和接穗品种红樱1号,研究植物生长调节剂对番茄穴盘育苗的影响。结果表明,不同植物生长调节剂对不同品种... 为筛选出适宜浙南地区番茄壮苗培育的植物生长调节剂,以不同浓度的YELVSU P2-37、多效唑、矮壮素、壮苗1号处理番茄砧木品种浙砧7号和接穗品种红樱1号,研究植物生长调节剂对番茄穴盘育苗的影响。结果表明,不同植物生长调节剂对不同品种番茄的处理效应不同,除对红樱1号茎粗、地下部干重和展叶宽无显著影响外,不同植物生长调节剂对红樱1号和浙砧7号的其他指标都有显著影响。所有生长调节剂对番茄株高都有抑制作用,但对其他指标不同生长调节剂作用不同。对于红樱1号,0.2 g·L^(-1)YELVSU P2-37处理的各生物量指标最高,同时种苗质量也最佳,为该品种壮苗培育的最佳植物生长调节剂;对于浙砧7号,0.13 g·L^(-1)YELVSU P2-37处理植株形态和种苗质量均为最佳,为该品种的推荐植物生长调节剂和适用浓度。 展开更多
关键词 番茄 植物生长调节剂 穴盘育苗 浙南
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盐胁迫下复合微生物肥料对西红柿的促生作用 被引量:2
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作者 李雪菲 孙一凡 孟建宇 《工业微生物》 CAS 2024年第2期124-127,共4页
文章旨在研究在盐胁迫条件下,复合微生物肥料对西红柿生长的影响。通过测定西红柿茎长、根长、地上部以及地下部干重等植物生长指标,分析该微生物菌肥对于植物的促生效果。结果表明,F5使株高提高了100.7%、速效磷提高了101.4%;M3使植株... 文章旨在研究在盐胁迫条件下,复合微生物肥料对西红柿生长的影响。通过测定西红柿茎长、根长、地上部以及地下部干重等植物生长指标,分析该微生物菌肥对于植物的促生效果。结果表明,F5使株高提高了100.7%、速效磷提高了101.4%;M3使植株根的鲜重增加了126.7%,速效磷提高了126.1%。结果表明,在盐胁迫条件下,复合微生物肥料能够对西红柿起到很好的促生作用,为盐碱地的开发利用提供了借鉴的依据。 展开更多
关键词 盐胁迫 微生物肥料 西红柿 促生作用
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三株无量山森林土壤芽孢杆菌鉴定及其生物活性挖掘 被引量:1
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作者 王楠 廖永琴 +7 位作者 施竹凤 申云鑫 杨童雨 冯路遥 矣小鹏 唐加菜 陈齐斌 杨佩文 《生物技术通报》 CAS CSCD 北大核心 2024年第2期277-288,共12页
【目的】挖掘无量山国家自然保护区潜在的高活性微生物菌株,旨在为农业绿色投入品研发提供高效的菌种资源。【方法】以番茄枯萎病菌(Fusarium oxysporum)为靶标筛选高拮抗活性菌株,观察对菌丝生长的影响,测定对孢子萌发的抑制作用;检测... 【目的】挖掘无量山国家自然保护区潜在的高活性微生物菌株,旨在为农业绿色投入品研发提供高效的菌种资源。【方法】以番茄枯萎病菌(Fusarium oxysporum)为靶标筛选高拮抗活性菌株,观察对菌丝生长的影响,测定对孢子萌发的抑制作用;检测脂肽类化合物合成基因以及菌株体外产酶、解磷、解钾、固氮及产铁载体能力;结合形态学、生理生化特征和16S rRNA、gyrA、rpoB基因进行菌株鉴定,室内盆栽试验验证防病和促生效果。【结果】分离筛选获得153株可培养细菌,其中,菌株SH-53、N4471和N9456抑菌效果分别为92.35%、87.29%和88.47%,能抑制病原菌菌丝生长和分生孢子萌发,且对多种病原菌表现出良好拮抗活性。3株功能菌均具有溶锌、产淀粉酶和蛋白酶的能力,菌株SH-53和N4471还具有解磷、固氮、分泌纤维素酶以及产铁载体能力。3个功能菌基因组中均含有srfA、fenA、ituA、ituC、ituD、bymC等脂肽类化合物合成基因。经鉴定菌株SH-53为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),N4471为卡式芽孢杆菌(B.cabrialesii),N9456为暹罗芽孢杆菌(B.siamensis)。盆栽试验结果表明,3株功能菌防效分别为84.66%、54.96%和59.74%,对番茄幼苗的株高、茎粗、根长等农艺性状均具有较好促进作用。【结论】3个菌株具有高效、广谱的抑菌活性,具有多样化的生物活性,作为高效的微生物资源具有广阔的应用前景。 展开更多
关键词 解淀粉芽孢杆菌 卡式芽孢杆菌 暹罗芽孢杆菌 鉴定 生物活性 番茄枯萎病 促生
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多粘类芽胞杆菌新菌株X-11及其对番茄和水稻的促生效应 被引量:1
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作者 刘文志 贺丹 +4 位作者 李鹏 傅应林 张译心 温华杰 于文清 《生物技术通报》 CAS CSCD 北大核心 2024年第9期249-259,共11页
【目的】从小麦(Triticum aestivum L)根际筛选到了一株细菌菌株(编号X-11),为该菌株在植物促生上应用提供依据。【方法】结合形态特征、生理生化特性、16S rDNA序列及基因组测序对其进行了鉴定,并测定其对番茄(Solanum lycopersicum)... 【目的】从小麦(Triticum aestivum L)根际筛选到了一株细菌菌株(编号X-11),为该菌株在植物促生上应用提供依据。【方法】结合形态特征、生理生化特性、16S rDNA序列及基因组测序对其进行了鉴定,并测定其对番茄(Solanum lycopersicum)和水稻(Oryza sativa)的促生效应。【结果】该菌株菌落圆形、半透明、凸起、黏稠;菌体杆状,形成芽胞,革兰氏阴性;具有固氮,还原硝酸盐,产吲哚、分泌铁载体及分解有机磷的功能。基于16S rDNA序列比对将X-11菌株归类为多粘类芽胞杆菌种群,基于非冗余蛋白数据库的基因比对表明其与最近缘菌株基因相似率55.7%,证明其为一个新菌株。X-11菌液浸种显著增加了番茄发芽率和胚根伸长度,灌根(R)及灌根结合叶面喷施(R+L)显著提高了番茄叶片中过氧化物酶(POD)活性,R+L处理使超氧化物歧化酶(SOD)活性提高了34.58%。水稻1叶1心期施用X-11菌液,根长增加了6.8%;立针期或1叶1心期施用X-11菌液,使秧苗SOD活性分别提高了90.4%和51.8%。【结论】X-11被鉴定为多粘类芽胞杆菌新菌株,是多种功能的植物根际促生菌,在植物促生方面具有开发应用潜力。 展开更多
关键词 多粘类芽胞杆菌 植物根际促生菌 番茄 水稻
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不同生育期番茄植株根际土壤微生物群落结构特征 被引量:1
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作者 魏宇飞 覃仁柳 +5 位作者 丁点草 黎永斌 谢源源 屈达才 赵天义 杨尚东 《华中农业大学学报》 CAS CSCD 北大核心 2024年第1期9-21,共13页
为构建番茄生态优质高产栽培技术体系及挖掘有益的微生物资源,比较了不同生育期番茄植株根际土壤肥力以及微生物群落结构与功能特征。基于传统与现代高通量测序技术分析番茄幼苗期、花芽分化期、结果期根际土壤肥力与微生物(细菌、真菌... 为构建番茄生态优质高产栽培技术体系及挖掘有益的微生物资源,比较了不同生育期番茄植株根际土壤肥力以及微生物群落结构与功能特征。基于传统与现代高通量测序技术分析番茄幼苗期、花芽分化期、结果期根际土壤肥力与微生物(细菌、真菌)群落结构与功能特征。结果显示,番茄结果期根际土壤中具有更为丰富的碳、氮、磷源;另一方面,不同生育期番茄根际土壤微生物群落结构和功能发生显著变化。其中,norank_f_norank_o_norank_c_KD4-96,norank_f_A4b和Bryobacter属细菌是幼苗期番茄植株根际土壤中特有的优势细菌属;TM7a和Saccharomonospora属细菌是花芽分化期番茄植株根际土壤中特有的优势细菌属;Gem⁃matimonas属细菌是结果期番茄植株根际土壤中特有的优势细菌属;此外,鲍尔壶菌属(Powellomyces)真菌是幼苗期番茄植株根际土壤中特有的优势真菌属;Apiotrichum和unclassified_f_Chytridiaceae真菌是花芽分化期番茄植株根际土壤中特有的优势真菌属;链格孢属(Alternaria)真菌是结果期番茄植株根际土壤中特有的优势真菌属。结果表明:不同生育期番茄植株根际土壤中形成了特异的根际土壤微生物群落,幼苗期根际土壤中主要富集了可以产生生长激素的微生物类群;花芽分化期根际土壤中主要富集了具有抗逆功能的微生物类群;结果期根际土壤中主要富集了具有促进养分循环功能的微生物类群。 展开更多
关键词 番茄 生育期 根际 微生物群落 高通量测序
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不同土壤改良技术对设施番茄生长及果实品质的影响
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作者 哈蓉 陈永伟 +3 位作者 靳韦 徐灿 张敏 王昊 《北方园艺》 CAS 北大核心 2024年第17期43-50,共8页
以大果番茄“普罗旺斯”为试材,采用测定多项生理指标并进行主成分分析的方法,研究了不同土壤改良技术对设施番茄营养生长和果实品质的影响,以期为提高设施番茄生长发育、改善果实品质提供参考依据。结果表明:土壤中阳离子交换量、碱解... 以大果番茄“普罗旺斯”为试材,采用测定多项生理指标并进行主成分分析的方法,研究了不同土壤改良技术对设施番茄营养生长和果实品质的影响,以期为提高设施番茄生长发育、改善果实品质提供参考依据。结果表明:土壤中阳离子交换量、碱解氮、速效钾、钙等微量元素和有机质含量显著增加,SH效果较好。番茄株高、茎粗、叶片面积的生长发育速率均高于对照;根系长度、粗度在SH处理下最好,根系鲜质量、干质量在SB处理下最高。各试验处理均增加了果实纵横径和产量,EB效果最为明显,产量增加40.98%;果实中糖分和营养物质含量均高于对照,氨基酸总量以SH处理最高为10.35 g·kg^(-1),营养价值最高。结合主成分综合分析,在SH处理下番茄生长和品质最佳。综上,设施内应用土壤改良技术,可改善土壤理化性质,缓解土壤障碍,对番茄根系和植株有一定促生作用,以太阳热处理效果最佳。 展开更多
关键词 土壤改良 设施番茄 生长发育 营养品质 氨基酸
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促生菌剂对番茄幼苗生长的影响
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作者 曹彩红 张敬锁 +3 位作者 田雅楠 张松阳 王忠义 曹玲玲 《中国农学通报》 2024年第1期33-37,共5页
为探明不同促生菌对番茄幼苗生长以及品质的影响,选择巨大芽孢杆菌、枯草芽孢杆菌、长枝木霉菌、蕈状芽孢杆菌和加纳木霉菌5种菌剂,以等量清水为对照,对幼苗的发芽、长势和抗逆性相关指标等进行调查测定,并通过隶属函数法进行综合分析... 为探明不同促生菌对番茄幼苗生长以及品质的影响,选择巨大芽孢杆菌、枯草芽孢杆菌、长枝木霉菌、蕈状芽孢杆菌和加纳木霉菌5种菌剂,以等量清水为对照,对幼苗的发芽、长势和抗逆性相关指标等进行调查测定,并通过隶属函数法进行综合分析。结果表明,在施用巨大芽孢杆菌、枯草芽孢杆菌和加纳木霉菌后,番茄幼苗生长均显著提高,菌剂利于植株干物质积累,与对照相比,干物质积累量增加了22.9%、45.7%和25.7%,根系活力提高89.0%、61.76%和41.3%。同时,施用巨大芽孢杆菌、枯草芽孢杆菌和加纳木霉菌的番茄苗,POD、SOD和CAT等抗氧化物酶活性显著提高,丙二醛含量显著降低,幼苗冬季生产抗寒性显著提高。基于隶属函数法的综合分析表明,巨大芽孢杆菌、枯草芽孢杆菌和加纳木霉菌适宜番茄壮苗培育。 展开更多
关键词 番茄 幼苗 促生菌剂 长势 抗逆性
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饲粮不同比例番茄秸秆替代花生秧对肉兔生长性能、营养物质表观消化率和盲肠微生物结构的影响
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作者 徐建 季荣梅 +4 位作者 张莉蕊 刘永需 赵红 刘磊 李福昌 《动物营养学报》 CAS CSCD 北大核心 2024年第6期3920-3930,共11页
本试验旨在研究饲粮不同比例番茄秸秆替代花生秧对肉兔生长性能、营养物质表观消化率和盲肠微生物结构的影响。选取体重相近的140只60日龄肉兔(公母各占1/2),按照平均体重分为4组(每组17或18只公兔,每组35个重复,每个重复1只兔),分别饲... 本试验旨在研究饲粮不同比例番茄秸秆替代花生秧对肉兔生长性能、营养物质表观消化率和盲肠微生物结构的影响。选取体重相近的140只60日龄肉兔(公母各占1/2),按照平均体重分为4组(每组17或18只公兔,每组35个重复,每个重复1只兔),分别饲喂不同比例番茄秸秆替代花生秧的全价配合饲料,替代比例分别为0(对照组)、5%(TS5组)、10%(TS10组)和20%(TS20组)。预试期3 d,正试期31 d。结果显示:饲粮中添加番茄秸秆对肉兔平均日采食量、脏器指数和盲肠微生物结构均无显著影响(P>0.05)。饲粮中番茄秸秆的添加降低肉兔死亡率和腹泻率。与对照组相比,TS5组和TS10组肉兔的平均日增重和料重比没有显著改变(P>0.05),但是TS20组平均日增重显著降低(P<0.05),TS20组料重比显著升高(P<0.05)。随着番茄秸秆替代比例的增加,背最长肌黄度值呈现先升高后降低的趋势,TS10组黄度值最高。与对照组相比,TS5组和TS10组的小肠长度显著增加(P <0.05),TS20组没有显著变化(P>0.05)。与对照组相比,番茄秸秆的添加显著提高粗脂肪、中性洗涤纤维和粗灰分表观消化率(P<0.05),TS5组粗蛋白质表观消化率显著提高(P<0.05)。饲粮中添加番茄秸秆并未对肉兔盲肠微生物结构产生显著影响(P>0.05)。综上所述,饲粮中用5%~10%的番茄秸秆替代花生秧能够降低肉兔腹泻率和死亡率,调节小肠发育,提高饲粮中部分营养物质的消化率,建议饲粮中番茄秸秆替代比例不超过10%。 展开更多
关键词 肉兔 番茄秸秆 生长性能 营养物质表观消化率 盲肠微生物
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