The Yield and Diseases-Pest Performance of Breeding Materials for Tomatoes (Lycopersicon esculentum) Sourced from Different Exotic Sources

A study was conducted with 36 tomato germplasm [PGRC (19), The Netherlands (7), Japan (2), and Bangladesh (9)] with 3 check varieties in the research field of the Olericulture Division of Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur during the winter season of 2022-23 to identify promising tomato breeding germplasm. All the germplasm showed differences in most of the parameters studied. Considerable variation was observed for fruit yield per hectare varied from 39 to 144 tons, while the highest fruit yield per hectare was harvested from the germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19. The number of locules, total soluble solids (TSS) and plant height at last harvest varied from 2 to 6.6, 2.6 to 5.9%, and 66 to 154cm, respectively. The TYLCV infection (%) and leaf-sucking pest infestation (%) were observed with a 1 to 20% range in the field condition in both cases. So, we can select that germplasm has zero per cent infestation. Considering the plant growth habit, earliness, different yield contributing horticultural traits, virus and pest infestation thirteen germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19 were selected as breeding materials for further tomato improvement program.

Study on Salinity Tolerance of Tomatoes during Seed Germination under Different Salt Stress Conditions

[Objective] The aim of this study was to provide the theoretical basis for screening and utilizing salt-tolerant tomato varieties as well as for cultivating salt-resistance.[Method] Salinity tolerance of tomato during seed germination under simple salt sodium chloride and double salt calcium nitrate or sodium chloride stress were studied by Petri dish culturing.[Result] As the two kinds salt concentration increased,the germination regularity,the germination rate,the germination index and the growing vigor index of tomato seedlings decreased,but the germination losing rate increased.When salt concentration was from 0.2% to 0.4%,there was little difference among all indexes under two kinds of salt stress.However,when salt concentration was from 0.6% to 1.0%,the difference among all indexes under two kinds of salt stress was significant.[Conclusion] Salinity tolerance of tomato seeds under double salt calcium nitrate or sodium chloride stress was higher than that under simple salt sodium chloride stress.

Studies on the Inheritance of Locule Formation in Tomatoes (Lycopersicon esculentum Mill.)

To fully understand the tomato ovary locule formation of inheritance, two varieties, ovary multi-locule （MLK1） and few-locule （FL1）, were used in these studies. Using reciprocal cross, self-cross, and backcross, we have constructed various groups to seek the genetic law and mechanisms of tomato locule formation, for modifying the tomato breeding theory. The parental and the resulting FI and F2 generations have been used to examine the heredity of the locule number. The results showed that few-locule was incompletely dominant. The data from backcross demonstrated a significant effect on the locule number, whereas, the reciprocal cross showed an insignificant effect. Not surprisingly, the locule number was controlled by nuclear genomes, not by exogenous substances. The model that inherited the locule number belonged to the additive-dominant model： additive effects played a very important role and were partially dominant. In addition, the results revealed that the locule number in tomato was mainly controlled by a single gene, whereas, it was modulated by a number of other genes. Finally, the general inheritability and narrow inheritability of the locule number were 69.44% and 52.98%, respectively.

Characterization of the Causal Organism of Soft Rot of Tomatoes and Other Vegetables and Evaluation of Its Most Aggressive Isolates

To isolate the causal organism of soft rot of vegetables, diseased samples of potato, tomato, carrot, chilies, and bell pepper, were analyzed in the lab, using nutrient agar (NA) and/or the enrichment host (Bell pepper) technique. Successful isolations were purified by sub-culturing, identified as Erwinia carotovora subsp. carotovora through biochemical tests and their pathogenicity was confirmed through inoculation on green tomato fruits. The isolates were tested for their aggressiveness to find out the most aggressive one in term of producing maximum soft rot on tomatoes. CRD (completely randomized design), with four replication was used and data were analyzed using LSD (least significant test) test. Among the five isolates evaluated for aggressiveness on tomato fruits, chili isolate was found to be the most aggressive followed by tomato and potato isolates producing 22.3 mm, 7.9 mm, and 7.8 mm diameter soft rot lesions, respectively.

Main and Trace Element Contents of Tomatoes Grown in Austria

Tomatoes are one of the most popular and widespread kinds of vegetables on a global scale. Tomatoes of various varieties, sizes and shapes, were grown in 3 different greenhouses in Austria on solid substrates. After freeze drying, they were analyzed for main and trace elements by ICP-OES, ICP-MS, and combustion methods （for C, N）. Main inorganic cation was K, whereas contents of hazardous metals （e.g. Cd, Pb）, Rare Earth Elements, Si, and Ti were marginal. Due to high water contents, the contribution to the Recommended Daily Intake of essential elements is largely below 1/5 for 1 kg consumption. In spite of different varieties grown at the 3 sites, trends for concentrations versus fruit size could be noted for K, Ca, S, B, Cu, Fe, Zn and Si. Similarly, fruits with seeds ＋ jelly tended to contain more of almost all elements, but less of Li, Ca, Sr, and Ba, whereas P and B were equally distributed. No significant correlations between the concentrations found in tomato-fruits and the amount of soil mobilizable in 0.16 M acetic acid （exchangeable ＋ acid mobile） were found. A look into already published data from other countries reveals that element concentrations met in tomatoes presumably depend on climatic conditions and suitably adapted varieties than on the growing substrate.

Use of By-Products from the Tequila Industry. Part 12: Composted Agave Bagasse for Growing Grape Tomatoes

Here, we evaluated the possibility of growing grape tomatoes on three different mixtures of composted agave bagasse substrate. Tomatoes were grown in 28-L pots for 106 days under a drip irrigation system with a hydroponic crop nutrient solution. The average production of grape tomatoes was 338.9, 358.9, and 325.7 g/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean of numbers of grape tomatoes were 34.6, 38.8, and 34.2/pot/cut for each of the substrates studied, and no significant difference was observed between treatments (p > 0.5). The mean weight of individual grape tomatoes was about 10.4 g for all of the substrates tested. These results confirmed the notion that a composted agave bagasse substrate could replace an expensive imported substrate. The remaining challenge is to produce agave bagasse substrate commercially, because to date, agave bagasse substrates have only been produced on a pilot scale for testing. Therefore, it is necessary to implement a stabilized process for producing agave bagasse at a commercial level. This process must consider economic production costs and market value to ensure that the product is competitive with other substrates typically used in soilless crops.

Prevalence of Salmonella in Fresh Produce Tomatoes Sold in Open Market in Ouagadougou, Burkina Faso

Tomato is a widely consumed vegetable in Burkina Faso and throughout the world. However, when handled improperly, it can serve as a vector of pathogen transmission to human if ingested raw or through cross-contamination with cooked food. This study aimed to determine the prevalence of Salmonella spp from tomatoes sold in the markets of Ouagadougou, Burkina Faso and characteristics of vending conditions. One hundred and ninety-eight (198) different tomato samples were randomly purchased from one hundred and thirteen (113) different vendors in the markets of Ouagadougou. The samples were examined and classified into three categories according to their texture and the level of deterioration. The prevalence of Salmonella spp identification was determined using standard microbiological methods. A survey was conducted using a questionnaire on transport and vending conditions for tomatoes. In total, Salmonella prevalence was 19.19% in all tomato’s samples analyzed and was 18.18% for intact category, 22.73% in D2 category and 16.67% in category D4. The survey revealed poor transport and sales conditions for the majority of tomato sellers. The present study revealed the presence of Salmonella in tomatoes samples. These results highlight the risk associated with the consumption of tomatoes if hygienic practices are not respected during tomatoes handling and preparation. The results of the study also show the need to wash tomatoes systematically after purchase with a disinfectant, especially tomatoes sold in open markets in developing countries.

Estimating the Efficiency of Summer Tomatoes Production: A Case Study in Borg El-Arab Area, Egypt

Tomatoes are one of the most popular and widely consumed vegetable crops worldwide. Egypt is characterized by its tomatoes production, whether for domestic use or export. Summer tomato farmers’ income has recently been reduced due to high production costs, particularly in new lands. As a result, the purpose of this research is to evaluate the economic efficiency of the elements used in summer tomato production in the Borg El-Arab area of Egypt as a new land. According to the findings, the summer tomato crop produced a reasonable profit for small-holding farmers in the study area. However, the elements are being used excessively in the production process, particularly seeds and fertilizers, resulting in increased production costs and failure to achieve economic efficiency of the elements used in the study area.

Exogenous GSH protects tomatoes against salt stress by modulating photosystem Ⅱ efficiency, absorbed light allocation and H2O2-scavenging system in chloroplasts

The effects of exogenous GSH（reduced glutathione）on photosynthetic characteristics,photosystem Ⅱ efficiency,absorbed light energy allocation and the H2O2-scavenging system in chloroplasts of salt-stressed tomato（Solanum lycopersicum L.）seedlings were studied using hydroponic experiments in a greenhouse.Application of exogenous GSH ameliorated saline-induced growth inhibition,the disturbed balance of Na＋ and Cl- ions and Na＋/K＋ ratios,and the reduction of the net photosynthetic rate（Pn）.GSH also increased the maximal photochemical efficiency of PSⅡ（Fv/Fm）,the electron transport rate（ETR）,the photochemical quenching coefficient（qP）,and the non-photochemical quenching coefficient（NPQ）.In addition,GSH application increased the photochemical quantum yield（Y（Ⅱ））and relative deviation from full balance between the photosystems（β/α-1）and decreased the PSⅡ excitation pressure（1-qP）and quantum yield of non-regulated energy dissipation（Y（NO））in leaves of salt-stressed tomatoes without BSO（L-buthionine-sulfoximine,an inhibitor of key GSH synthesis enzymeγ-glutamylcysteine synthetase）or with BSO.Further,the addition of GSH depressed the accumulation of H2O2 and malondialdehyde（MDA）,induced the redistribution of absorbed light energy in PSⅡ reaction centers,and improved the endogenous GSH content,GSH/GSSH ratio and activities of H2O2-scavenging enzymes（including superoxidase dismutase（SOD）,catalase（CAT）,peroxidase（POD）and key enzymes in the AsA-GSH cycle and Grx system）in the chloroplasts of salt-stressed plants with or without BSO.Therefore,GSH application alleviates inhibition of salt-induced growth and photosynthesis mainly by overcoming stomatal limitations,improving the PSⅡ efficiency,and balancing the uneven distribution of light energy to reduce the risk of ROS generation and to mediate chloroplast redox homeostasis and the antioxidant defense system to protect the chloroplasts from oxidative damage.Thus,GSH may be used as a potential tool for alleviating salt stress in tomato plants.

Effect of Different Calcium Levels on Growth,Yield and Fruit Quality of Tomatoes in Substrate Culture

Tomato seedlings were grown in substrate culture with pots. The formulation of Holland Greenhouse Horticulture Research Institute was used(as CK) and the effects of different Ca concentrations(LCa, CK and HCa)on growth, yield, and fruit quality(protein, Vitamin C, nitrates, organic acid and soluble sugar) of tomato were studied keeping concentrations of other nutrients unchanged in the nutrient solution. The results showed that parameters related to the growth of tomato(plant height and Stem diameter), changes of tomato yield per plant and quality of tomato fruits were the highest when the plants were grown at 20% Ca treatments. In the second study, increased EC concentrations of nutrient solution resulted in stronger plants with improved yields and quality. Four different concentration gradients of nutrient solution treatment were designed based on the results of the first research stage(EC=1.5, 2.5, 3.5, 4.5 m S/cm, respectively). The single tomato plant had the highest production which is 2 268.994 g/plant, when the nutrient solution strength was at EC=1.5 m S cm, whereas they have the best fruit quality when the solution strength at EC 4.5 m S/cm. This suggests the need for wide popularization of the nutrient solution formula in large areas to improve the tomato production.

Effects of biodegradable mulch on soil water and heat conditions,yield and quality of processing tomatoes by drip irrigation

To combat the problem of residual film pollution and ensure the sustainable development of agriculture in oasis areas,a field experiment was carried out in 2019 at the Wuyi Farm Corps Irrigation Center Test Station in Urumqi,Northwest China.Four types of biodegradable mulches,traditional plastic mulchs and a control group(bare land;referred to as CK)were compared,including a total of six different treatments.Effects of mulching on soil water and heat conditions as well as the yield and quality of processing tomatoes under drip irrigation were examined.In addition,a comparative analysis of economic benefits of biodegradable mulches was performed.Principal component analysis and gray correlation analysis were used to evaluate suitable mulching varieties for planting processing tomatoes under drip irrigation.Our results show that,compared with CK,biodegradable mulches and traditional plastic mulch have a similar effect on retaining soil moisture at the seedling stage but significantly increase soil moisture by 0.5%-1.5%and 1.5%-3.0%in the middle and late growth periods(P<0.050),respectively.The difference in the thermal insulation effect between biodegradable mulch and plastic mulch gradually reduces as the crop grows.Compared with plastic mulch,the average soil temperature at 5-20 cm depth under biodegradable mulches is significantly lowered by 2.04°C-3.52°C and 0.52°C-0.88°C(P<0.050)at the seedling stage and the full growth period,respectively,and the water use efficiency,average fruit yield,and production-investment ratio under biodegradable mulches were reduced by 0.89%-6.63%,3.39%-8.69%,and 0.51%-6.33%(P<0.050),respectively.The comprehensive evaluation analysis suggests that the black oxidized biological double-degradation ecological mulch made from eco-benign plastic is the optimal film type under the study condition.Therefore,from the perspective of sustainable development,biodegradable mulch is a competitive alternative to plastic mulch for large-scale tomato production under drip irrigation in the oasis.

Correlation Between Endogenous Hormones of Stem Apices and Fruit Locule Numbers in Tomatoes During Floral Bud Differentiation Stages

The study was designed to elucidate the changes in the endogenous hormones of stem apices in tomatoes and the function of correlative endogenous hormones in tomatoes during floral bud differentiation stages. The tomato parents were crossed and reverse crossed by using two inbred lines of multi-locule （MLK1） and few-locule （FL1） with significant difference, and the relationship between endogenous hormones GA3, IAA, and ABA levels and ovary locule numbers of parents and progeny during floral bud differentiation initial stage, floral bud differentiation stage, sepal petal formation stage, carpel formation initial stage, and ovary locule complete formation stage was studied. GA3 levels in P1, P2, F1, and RF1 were consistent with locule numbers, and IAA and ABA levels were reverse to ovary locule numbers during the key stage. The correlation showed that, during sepal petal formation stage, the ovary locule numbers were positively correlated with GA3, GA3/IAA, and GA3/ABA, and were negatively correlated with IAA and ABA. It was speculated that increasing GA3 levels or decreasing IAA and ABA levels of stem apices in tomato might be able to enhance ovary locule numbers. The sepal petal formation stage was an important stage which regulated endogenous hormones in the ovary locule formation.

Effect of Organic and Chemical Fertilizer Application on Growth, Yield, and Quality of Small-Sized Tomatoes

Tomatoes in Japan are generally cultivated under management systems that use chemical fertilizers and synthetic chemical pesticides. However, the continuous use of these fertilizers and pesticides damages the soil environment and reduces the number of soil microorganisms. Organic farming has a relatively low environmental impact compared to conventional farming techniques, but typically has lower and more unstable yields. In this study, we investigated the effect of organic and chemical fertilizer application on growth, yield, and quality of small-sized (cherry) tomatoes. Cherry tomatoes were cultivated using organic and chemical organic fertilizers. Average weight and lateral diameter were significantly higher under organic fertilizer than under chemical fertilizer. In addition, shoot dry weight was significantly higher under organic fertilizer than chemical fertilizer. Lycopene content was significantly higher under organic fertilizer than chemical fertilizer. The total carbon (TC), total phosphorus (TP), total potassium (TK), available phosphoric (SP) and exchangeable potassium (SK) contents, C/N ratio, and pH were significantly higher under organic fertilizer than chemical fertilizer. Bacterial biomass, nitrite (NO? 2-N) oxidation activity, nitrification (N) circulation activity, and phosphoric (P) circulation were higher under organic fertilizer than chemical fertilizer. From these results, the study indicates that appropriate controls such as TC, total nitrogen (TN), and C/N ratio of organic fertilizer increased microbial biomass and enhanced nutrient circulation such as N circulation activity and P circulation activity. These results can be used to improve current organic farming practices and promote soil conservation.

Effect of organic fertilizers used in sandy soil on the growth of tomatoes

In order to reveal the regulating capacity of organic fertilizers on sandy soil, pots experiments were carried out. The growth of tomatoes planted on sandy soil amended by organic fertilizers was measured. Organic fertilizers can be helpful to improve the plant height, stem diameter, the aerial parts fresh weight, root fresh weight, leaf photosynthetic rates and photosynthesis, and lay a good foundation for the growth of ??tomatoes. The effect of organic fertilizer is the most significant. Among all the treatments of adding 2.5%, 5%, 10% organic fertilizers, adding 10% organic fertilizers are the best. It can significantly enhance the growth and photosynthesis of tomatoes, and it is among the best of these three soil treatments for sandy soil.

The Effect of High Day and Low Night Temperature on Pollen Production, Pollen Germination and Postharvest Quality of Tomatoes

Temperature integration where high day temperatures are compensated by lower night temperatures is one strategy that can be used to reduce energy consumption in greenhouses. Crop tolerance to temperature variation is a prerequisite for using such a strategy. Greenhouse experiments were conducted on tomatoes cvs, Capricia, Mecano and Cederico in order to investigate the effect of different day/night temperature regimes (24/17, 27/14 and 30/11℃) where the same mean temperature was maintained for the production and germination of pollen. In addition, fruit quality as determined by fruit firmness, dry matter content, soluble solids, titratable acids, and pH was examined at harvest and after seven and 14 days of storage. The 30/11℃ treatment significantly increased pollen production and germination compared to the 24/17℃ treatment, while the 27/14℃ treatment was generally in between the other two treatments. Fruits grown at the 27/14℃ treatment were significantly firmer, while fruits grown at 24/17℃ had higher dry matter content, soluble solids, and titratable acids compared to the other treatments. There were significant differences between cultivars with respect to firmness, dry matter, titratable acidity, and pH. The quality of the fruits changed during storage, but the storability of the tomatoes was not affected by preharvest temperature treatments. The overall conclusion was that the 27/14℃ treatment was superior to the other two temperature treatments with respect to the studied parameters.

Impacts of Sletr1-1 and Sletr1-2 mutations on the hybrid seed quality of tomatoes

Shelf life is an important breeding trait in tomato, especially for the tomato production in subtropical and tropical regions. Previously we have isolated and characterized ethylene receptor mutants, Sletr1-1 and Sletr1-2 from mutant population based on Micro-Tom cultivar. Sletr1-1 showed insensitivity to ethylene while Sletr1-2 showed reduced sensitivity to ethylene. We also have demonstrated that the traits are useful for extending fruit shelf life of the hybrid tomato cultivars. For commercializing the hybrid cultivars, the seed quality is another important trait. In this study, we evaluated the effects of the Sletr1-1 and Sletr1-2 mutations on the seed quality characteristics of F_1 hybrid lines generated by crossing Sletr1-1 and Sletr1-2 with three commercial tomato cultivars, Intan, Mutiara and Ratna. Sletr1-1 mutation conferred insensitivity to ethylene in the F_1 hybrid seedlings, resulting in negative effects including reduced germination rate, vigor index and emergence speed index. Interestingly Sletr1-2 mutation had almost no effect on the seed quality characteristics of the F_1 hybrid lines, suggesting that Sletr1-2 was suitable for producing high quality of hybrid seeds.

Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeERT1 and LeERT2

Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato （Lycopersicon esculenturn Mill.） on ethylene signaling. The transgenic line alel containing antisense LeER T1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B 1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to alel in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development.

Nitrogen application levels based on critical nitrogen absorption regulate processing tomatoes productivity, nitrogen uptake, nitrate distributions, and root growth in Xinjiang, China

The unreasonable nitrogen(N)supply and low productivity are the main factors restricting the sustainable development of processing tomatoes.In addition,the mechanism by which the N application strategy affects root growth and nitrate distributions in processing tomatoes remains unclear.In this study,we applied four N application levels to a field(including 0(N0),200(N200),300(N300),and 400(N400)kg/hm^(2))based on the critical N absorption ratio at each growth stage(planting stage to flowering stage:22%;fruit setting stage:24%;red ripening stage:45%;and maturity stage:9%).The results indicated that N300 treatment significantly improved the aboveground dry matter(DM),yield,N uptake,and nitrogen use efficiency(NUE),while N400 treatment increased nitrate nitrogen(NO_(3)^(-)-N)residue in the 20–60 cm soil layer.Temporal variations of total root dry weight(TRDW)and total root length(TRL)showed a single-peak curve.Overall,N300 treatment improved the secondary root parameter of TRDW,while N400 treatment improved the secondary root parameter of TRL.The grey correlation coefficients indicated that root dry weight density(RDWD)in the surface soil(0–20 cm)had the strongest relationship with yield,whereas root length density(RLD)in the middle soil(20–40 cm)had a strong relationship with yield.The path model indicated that N uptake is a crucial factor affecting aboveground DM,TRDW,and yield.The above results indicate that N application levels based on critical N absorption improve the production of processing tomatoes by regulating N uptake and root distribution.Furthermore,the results of this study provide a theoretical basis for precise N management.

The antithrombotic activity of mini-type tomatoes is dependent on the particular variety and the stage of harvest. Lycopene content does not contribute to antithrombotic activity

The prevention of arterial thrombotic disease has a high priority in developed countries. We have focused our studies on the antithrombotic activity of those fruits and vegetables with the potential to prevent the disease, and the present study was undertaken as part of a series of investigations to examine beneficial fruits and vegetables. For this purpose, suitable laboratory tests as well as diets have been devised. In the current investigation, we have classified various tomato varieties with antithrombotic properties, and we now have extended our overall data to include more than ten antithrombotic varieties of fruits and vegetables. A method designed to measure shear-induced platelet activity (the Global Thrombosis Test, GTT) was used to assess haemostasis in vitro and a He-Ne laser-induced thrombosis technique was utilized to examine arterial thrombogenesis in vivo. Concentrations of the antioxidant, lycopene, were also measured. Three mini-type tomato varieties, coded “Cin”, “Pik” and “Caec”, and one mediumtype variety, coded “K”, were harvested at different stages of maturity. All mini-type varieties demonstrated antithrombotic activity at an early (green) stage. The antithrombotic activity decreased with the maturation of “Cin” and “Caec” but remained constant at all stages of maturity with “Pik”. The medium variety, “K”, did not possess antithrombotic activity. Lycopene was not detected at any stage in any of the tomato varieties, suggesting that this antioxidant did not contribute to antithrombotic activity. The present results indicated that the antithrombotic activity of tomatoes is dependent on the particular variety and stage of maturity, and that this activity is not due to lycopene.

Detection of Potato Spindle Tuber Viroid, and Its Impact on Growth, Production of Tomatoes in Greenhouse

The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes （PSTVd） and its impact on growth and production of some of these varieties, which were mechanically inoculated with Libyan isolate of viroid PSTVd as follows： Vlkato, Sankarh, Lebda, Jasmine, Kenza and Hana. The percent of incidence were 95.95%, 90%, 90.80%, 80% and 20%, respectively. The following varieties have been contagious mechanically with viroid of PSTVd： Vlkato, zahra, Toria, Lebda, Hoda, Farwa, Alkaraz, Naziha, Rim Star and Kartika. The percent of incidence were 95.95%, 85%, 85.80%, 80%, 70.40%, 0.0%, 0.0%, respectively. The varied symptoms of wrinkle, twist, warp, swell the veins of the leaves, dark brown spots formation and a large yellow spots turned into white patches. Also the effect of the Egyptian isolate viroid PSTVd in the growth and production of varieties Jasmine, Lebda, Soberhalim, and treasure No. 185 had been studied, as the average rates of decline in the production of the fruits tomatoes/tomato 43.4% and 17% length of plants, and in the fresh weight and dry root of the sum of 35% and 37% respictively.