Resistance development,cross-resistance,and fitness costs associated with Aphis gossypii resistance towards sulfoxaflor and acetamiprid in different geographical regions

Aphis gossypii has become increasingly difficult to manage due to its strong insecticide resistance.In the laboratory,we established sulfoxaflor-resistant and acetamiprid-resistant strains in two A.gossypii populations with different basal insecticide resistance levels,and evaluated the effects of basal insecticide resistance on the resistance development and cross-resistance,as well as differences in fitness.Under the same selection pressure,Yarkant A.gossypii(with low basal insecticide resistance)evolved resistance to sulfoxaflor and acetamiprid more quickly than Jinghe A.gossypii(with high basal insecticide resistance),and the evolution of A.gossypii resistance to sulfoxaflor developed faster than acetamiprid in both Yarkant and Jinghe,Xingjiang,China.The sulfoxaflor-resistant strains selected from Yarkant and Jinghe developed significant cross-resistance to acetamiprid,imidacloprid,thiamethoxam and pymetrozine;while the acetamiprid-resistant strains developed significant cross-resistance to sulfoxaflor,imidacloprid,thiamethoxam,pymetrozine,and chlorpyrifos.The relative fitness of A.gossypii decreased as the resistance to sulfoxaflor and acetamiprid developed.The relative fitness levels of the sulfoxaflor-resistant strains(Yarkant-SulR and Jinghe-SulR)were lower than those of the acetamipridresistant strains(Yarkant-AceR and Jinghe-AceR).In addition,the relative fitness levels of sulfoxaflor-and acetamiprid-resistant strains were lower in Jinghe than in Yarkant.In summary,basal insecticide resistance of A.gossypii and insecticide type affected the evolution of resistance to insecticides in A.gossypii,as well as cross-resistance to other insecticides.The sulfoxaflor-and acetamiprid-resistant A.gossypii strains had obvious fitness costs.The results of this work will contribute to the insecticide resistance management and integrated management of A.gossypii.

Chemically Mediated Interactions between Grapevine, Aphid, Ladybird, and Ant in the Context of Insect Chemical Ecology

This study simplifies the complex relationship among grapevine plants,aphids,ladybirds,and ants,which is essential for effective pest management and ecological balance.This study investigated the impact of aphid attacks and the presence of ants and ladybirds on the volatile compounds profile released into the chemosphere of the community consisting of the common vine Vitis vinifera,the aphid Aphis illinoisensis,the ladybird Cocci-nella undecimpunctata-and the ant Tapinoma magnum.This study aims to analyze the volatile compounds emitted by the grapevine and surrounding insects in response to these intricate interactions.The extraction of volatile organic compounds(VOCs)was carried out using closed-loop stripping(CLS)and then analyzed via gas chromatography-mass spectrometry(GC-MS)and principles coordinated analysis(PCA)was performed.The grapevine was exposed to different types and order of treatments,including non-infested,aphid-infested,aphid-infested with ant,aphid-infested with ladybird,and various combinations of ant and ladybird.After the aphid attack,the outcomes uncovered massive alterations in the volatile compound profiles.Infested grapevine displayed distinct emissions of germacrene D,an alcohol,and an alkene compared to non-infested plants.The characteristic VOC profile was the share of infested grapes in the presence of ants,with benzene derivatives and sesquiterpenes dominating the components.The coexistence of ladybirds with ants and aphids resulted in a dif-ferent volatile profile characterized by elevated levels of aldehydes,ketones,α-farnesene,and its hydroxy deriva-tive.It was concluded that the emission of VOCs into the chemosphere of the grapevine communities varied qualitatively and quantitatively depending on the level of the relationship complexity within each community in response to the infestation of grapevines by aphids,the presence of ladybirds as natural predators,and the presence of ant as protector.The grapevine’s status-dependent compounds can serve as indicators of infestation status and contribute to non-destructive early-stage diagnosis of the aphid.

Performance in Survival,Development and Reproduction of Aphis glycines Matsumura Virginoparae at High Temperatures

The soybean aphid,Aphis glycines Matsumura,is an important pest of soybean,which is native to Asia.In this study,A.glycines fed on soybean(AgFS)and A.glycines fed on wild soybean(AgFW)were reared at 25℃,27℃,29℃,31℃,33℃and 35℃,respectively,and some of the life parameters were determined.At temperature ranging from 25℃to 31℃,nymphs of AgFS and AgFW all developed into adults successfully.Only a few nymphs of AgFS and AgFW developed into adults at 33℃and no nymphs could develop into adults at 35℃.Lifespan,fecundity and body size of AgFS and AgFW adults all decreased gradually with temperatures increasing from 25℃to 33℃.At 25℃,the intrinsic rate of increase of AgFS was as big as that at 27℃,which was smaller than that at 29℃,but was bigger than that at 31℃.Intrinsic rate of increase of AgFW decreased gradually with temperatures increasing from 25℃to 31℃.Nymph stage duration of AgFW was longer than or as long as that of AgFS;adult lifespan of AgFW was shorter than or as long as that of AgFS.Adult fecundity,intrinsic rate of increase and adult body size of AgFW were all smaller than or as big as those of AgFS.It showed that AgFS and AgFW both survived and developed well at temperature ranging from 25℃to 31℃,and AgFW was more adaptive to low temperatures.These results were important to study the adaptability of A.glycines to high temperatures and for predicting its dynamics in the temperature keeping rising region.

温度对十斑大瓢虫产卵特性的影响

本研究旨在探究不同温度条件对十斑大瓢虫产卵特性的影响,以为其规模化繁育与应用提供理论支撑。实验设计了3种不同的温度处理:25℃恒温、25/30℃变温、30℃恒温,试验过程记录各处理中十斑大瓢虫产卵前期、产卵量、孵化率及成虫体重等指标。研究结果显示,在为期30天的饲养周期内,3种温度处理下十斑大瓢虫的产卵量没有显著性差异;产卵前期及雌雄虫体重没有显著性差异。产卵开始后第10天和第25天,25℃恒温环境下的卵孵化率显著高于30℃恒温、25/30℃变温饲喂组卵的孵化率(P<0.05)。在30 d的观察期内,各处理组间的平均卵孵化率没有显著性差异。本研究表明,25/30℃变温环境和30℃恒温环境在一定程度上显著降低了十斑大瓢虫卵的孵化率,但对其产卵前期、产卵量及成虫体重无显著影响。本研究结果为十斑大瓢虫的规模繁育和应用提供数据基础。

不同虫龄异色瓢虫对棉蚜的潜在控制能力评价

为明确天敌昆虫对黄瓜害虫的自然控制作用,以优势天敌昆虫异色瓢虫为对象,黄瓜棉蚜为猎物,采用HollingⅡ功能反应数学模型、寻找效应模型和HasselⅡ数学模型,研究不同虫龄异色瓢虫对黄瓜棉蚜的捕食功能反应。结果表明,在试验范围内,不同虫龄异色瓢虫对棉蚜的捕食量与棉蚜密度正相关,捕食功能曲线符合HollingⅡ模型,1~4龄异色瓢虫对棉蚜的最大日捕食量分别为31.25、66.225、192.308和370.370头,成虫最大日捕食量为124.20头;4龄幼虫对棉蚜的控制能力最强,a′/T_(h)值最高(372.119),其次为3龄幼虫、成虫、2龄幼虫,1龄幼虫对棉蚜的控制能力相对最弱,a′/T_(h)值最低(27.366)。不同龄期异色瓢虫的寻找效应与棉蚜密度均呈负相关,且在相同猎物密度下,4龄异色瓢虫对棉蚜的寻找效应相对最高。异色瓢虫成虫的捕食作用存在较强的种内干扰反应,拟合模型为E=0.6625P^(-0.8307)。综上表明,不同虫龄异色瓢虫对棉蚜均具有不同程度的捕食能力,尤其高龄幼虫和成虫捕食作用较强。

吡虫啉和苦参碱及其复配对毛叶苕子蚜虫的毒力测定及田间防效

为防治绿肥作物毛叶苕子上蚜虫危害,筛选出对毛叶苕子蚜虫高效配方,选择作用机理不同的吡虫啉和苦参碱,开展了最佳配比筛选及田间防治效果评价。结果表明,吡虫啉和苦参碱4种混配组合(有效成分含量之比分别为1∶10、1∶20、1∶40和1∶80)对毛叶苕子上棉蚜的毒力均表现为增效作用,其中1∶20组合的共毒系数最高,为376.14。选择该配比进行的田间药效试验表明,该混剂组合在有效成分用量为5.94 g/hm^(2)时,药后3、7 d的防治效果均显著高于单剂,最高防效近80%,可作为今后混剂的开发或生产中防治毛叶苕子蚜虫的推荐药剂。

南疆苹果园绣线菊蚜种群时空动态与调查抽样技术

绣线菊蚜Aphis spiraecola是新疆南疆地区苹果园的一种重要害虫,本文在阿克苏地区调查了绣线菊蚜在苹果园的种群消长动态,并分析其种群在果园的空间分布格局、理论抽样数及序贯抽样方法。结果表明,5月至8月苹果园中绣线菊蚜的种群动态曲线呈现出明显单峰,6月为种群发生的高峰期。在发生高峰期,绣线菊蚜在果园呈聚集型分布,种群密度在苹果树不同方位间没有显著差异。该时期苹果园绣线菊蚜的最适理论抽样模型为N=t^(2)/D^(2)(163.90/m+0.16),当防治阈值为1500头/12枝条时,序贯抽样模型公式为T_(n)=1500 n±1534√n。本研究结果为南疆苹果园绣线菊蚜种群调查与测报提供了重要科学依据。

棉蚜Aphis gossypii Glover在不同寄主上的体色分化研究

对不同寄主上不同时期棉蚜的体色分化进行比较研究 结果表明 ,棉蚜自然种群由黄色型和绿色型 2种个体组成 .在木槿、石榴和花椒 3种越冬寄主上 ,早春以绿色型个体为主 ,春末夏初黄色型个体逐渐占优势 ,夏末秋初又以绿色型个体为主 ;在夏季寄主棉花上 ,黄色型个体一直占优势 ,其中 5～ 6月份棉花苗期可见到部分绿色型个体 ,所占比例与越冬寄主上棉蚜迁出时所占的比例基本接近 .在室内不同温度条件下连续饲养不同体色棉蚜的试验结果表明 ,绿色型棉蚜可分化出绿色型和黄色型 2种后代 ,后代的体色变化与温度密切相关 ,在温度较高时黄色型后代占优势 ,在温度较低时绿色型后代占优势 ,而且黄色型后代随温度的降低也会产生出绿色型后代 .系统饲养黄色干母的后代还发现 ,不论饲养温度如何变化 ,其后代均为黄色型 ,表明自然界有

棉蚜对不同品种枸杞的适应性

为了明确棉蚜(Aphis gossypii Glover)对宁夏地区不同主栽枸杞品种的适应性,利用年龄-阶段两性生命表对棉蚜取食不同品种枸杞后生长发育及种群参数进行研究。结果表明,取食不同枸杞品种的棉蚜产蚜期、产蚜量和寿命存在显著差异。其中取食‘宁杞1号’的棉蚜产蚜期、总寿命、成虫寿命和产蚜量分别为8.61 d、15.22 d、9.58 d和11.78头,显著低于‘宁杞5号’(分别为9.71 d、16.14 d、10.78 d和15头)、‘宁杞7号’(分别为9.71 d、15.87 d、10.52 d和14.21头)和‘宁杞10号’(分别为11.08 d、17.80 d、12.24 d和17.40头)。取食不同枸杞品种的棉蚜净增殖率(R0)、总繁殖率(GRR)、内禀增长率(r)、平均世代周期(T)和周限增长率(λ)等参数存在显著差异。其中取食‘宁杞1号’棉蚜的R0、GRR、r和λ值(分别为7.07、14.13、0.21 d^(-1)和1.24 d^(-1))均显著低于‘宁杞5号’(分别为10.5、16.9、0.26 d^(-1)和1.29 d^(-1))、‘宁杞7号’(分别为11.37、18.47、0.27 d^(-1)和1.30 d^(-1))和‘宁杞10号’(分别为14.5、20.41、0.28 d^(-1)和1.32 d^(-1));取食‘宁杞1号’棉蚜的t值(9.17 d)显著低于‘宁杞10号’(9.5 d),高于‘宁杞7号’(9.01 d)。综上表明,棉蚜取食4个枸杞品种均能正常发育并完成整个世代,但在不同品种枸杞上的适应性不同,在‘宁杞10号’品种上的适应性最好,‘宁杞5号’和‘宁杞7号’次之,‘宁杞1号’的适应性最低。因此,可以考虑利用棉蚜适应性较低的品种作为宁夏地区主栽枸杞对棉蚜进行防控。

山东省主要菜区瓜(棉)蚜(Aphis gossypii Glover)抗药性及机理研究

利用毛细管点滴法测定了山东省 4个主要菜区瓜 (棉 )蚜 (Ap his gossypiiGlover)对氰戊菊酯、吡虫啉、氧乐果和灭多威的抗性。结果表明 ,聊城种群对氰戊菊酯的抗性高达 12 1710 .1倍 ,对吡虫啉、氧乐果和灭多威也分别产生了 9.6、2 9.4及 7.9倍的抗性 ;寿光种群对氰戊菊酯的抗性也高达 2 74 59.3倍 ,对吡虫啉、氧乐果和灭多威的抗性分别为 4 .1、18.1及 2 .9倍 ;苍山、泰安两种群对 4种药剂的抗性亦有不同程度的提高。离体酶活性和增效剂测定结果表明 ,聊城种群的抗药性与多功能氧化酶、羧酸酯酶、谷胱甘肽转移酶和乙酰胆碱酯酶活力提高均有关系。

棉蚜(Aphis gossypii)种群寄主分化和季节分化的微卫星引物PCR研究

通过对棉蚜群体进行室外观察、室内培养和分子遗传标记的 DNA多态性分析 (用 3个微卫星序列为引物进行PCR)等研究 ,结果表明 :1 .冬寄主 (木槿、石榴和花椒 )上棉蚜种群分化较小 (种群相似性指数在 0 .833～ 0 .91 7之间 ) ,而冬寄主种群和夏寄主 (棉花和南瓜 )种群之间有较大的分化 (种群相似性指数在 0 .589～ 0 .756之间 )。2 .棉蚜自然种群是由体色不变的生物型 (干母为黄色 ,其后代始终为黄色 )和体色可变型生物型 (干母为绿色 ,其后代有绿色和黄色两种体色 )组成的混合种群。3.黄色小型蚜 (伏蚜 )来源于混合类群 ,但主要来自绿色干母多代选择分化的黄色后代 。

金龟子绿僵菌CQMa421对棉蚜、绿盲蝽的田间防效

棉花是我国重要的经济作物,虫害严重制约了棉花生产,造成棉农的经济损失。目前,化学杀虫剂仍是防治棉花虫害的主要手段,长期使用化学杀虫剂导致棉蚜Aphis gossypii、绿盲蝽Apolygus lucorum产生抗药性,降低防治效果。本研究使用80亿孢子/mL金龟子绿僵菌CQMa421可分散油悬浮剂防治棉蚜、绿盲蝽,室内生测结果表明,金龟子绿僵菌CQMa421能有效侵染棉蚜和绿盲蝽,随着浓度的升高,对棉蚜和绿盲蝽的毒力增强。田间试验结果表明,该药剂能有效控制棉花主要害虫棉蚜和绿盲蝽。采用1200 mL/hm 2用量,药后14 d对棉蚜的防效达94.42%;采用1350 mL/hm 2用量,药后14 d对绿盲蝽的防效分别达到87.25%(新疆),91.62%(湖北)。

Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii

[Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and provide theoretical basis for studying the biosafety of transgenic cotton.[Method] Cotton aphids were fed with SGK321 and Shiyuan321（normal parental varieties） for over 40 generations.Enzyme activities were compared between cotton aphids feeding on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.[Result] The carboxylesterase activity of cotton aphids feeding on SGK321 for 1 generation was significantly higher than those feeding on Shiyuan321.Acetylcholinesterase activity of cotton aphids feeding on SGK321 for 1,2 and 3 generations were significantly higher than those feeding on Shiyuan321 in the same generation.But there was no significant difference of enzyme activity between cotton aphids feeding on SGK321 for a long term and those feeding on parental cotton.[Conclusion] The cotton aphid that feeding on transgenic Bt plus CpTI cotton SGK321 for a long time has adaptivity to SGK321 by regulating the detoxifying enzyme.

Cloning and Sequencing of Two Acetylcholinesterase cDNA Fragments from Cotton Aphid,Aphis gossypii Glover

Two acetylcholinesterase (AChE) genes cDNA fragments,Ag.acel and Ag.ace2,have been cloned from cotton aphid,Aphis gossypii Glover using degenerate primers with RT-PCR technique.Ag.acel gene cDNA fragment is of 282?bp encoding 94 amino acids,and Ag.ace2 gene cDNA fragment is of 264?bp encoding 88 amino acids.Both two putative AChE genes cDNA fragments share numerous similarities with those cloned from other insects.This is the first report of two AChE cDNA fragment sequences in the insect species,which provided the direct evidence of multiple AChE existence in insects.

助剂对植保无人机喷施雾滴沉积特性和棉蚜防效的影响

【目的】探明植保无人机喷施农药时添加助剂对雾滴沉积特性和棉蚜防治效果(防效)的影响。【方法】选用大疆T30植保无人机在棉花蕾期进行田间喷雾试验,比较添加6种助剂(牙克透、倍达通、植物三餐、农健飞、奇功和倍倍加)对39%螺虫·噻嗪酮悬浮剂的雾滴粒径、密度、覆盖率和沉积量及棉蚜防效的影响。【结果】与不添加助剂的对照相比,添加6种助剂均能提高棉花上部、中部和下部叶片上的雾滴密度、覆盖率和沉积量。添加倍倍加、倍达通处理的雾滴粒径、密度、覆盖率和沉积量均较大,棉株上部、中部和下部叶片上的雾滴密度、覆盖率和沉积量均显著高于对照处理。添加6种助剂的各处理在药后1 d、3 d、7 d和14 d对棉株上部、中部和下部棉蚜的防效均高于对照处理,其中,添加倍倍加处理的防效最好,倍达通处理次之。【结论】添加助剂倍倍加和倍达通可改善雾滴沉积特性,对棉蚜具有较好的防效,对39%螺虫·噻嗪酮悬浮剂防治棉蚜具有很好的增效作用。

不同温度对绣线菊蚜(Aphis citricola Van der Goot)实验种群的影响(英文)

在13,15,17,19,23,26,28℃七个温度梯度及相对湿度70%±10%的条件下饲养绣线菊蚜（Aphis citricola Van der Goot）,测定了其若虫各发育阶段的发育起点温度、有效积温及其种群生殖力参数。结果表明,在13～28℃温度范围内,蚜虫各发育阶段发育历期随温度的升高而缩短。绣线菊蚜在17℃时净增殖率最高,达到8.23。19℃的内禀增长率（0.09）最大,种群加倍时间（8.09 d）最短,绣线菊蚜的最适生长温度在19℃附近。高温和低温均不利于绣线菊蚜的生长发育,28℃时其各项繁殖力参数急剧下降。而在13℃下其若虫未能正常进入成虫发育阶段。

13种植物甲醇提取物对瓜蚜生物活性的影响

【目的】为了明确植物提取物对瓜蚜(Aphis gossypii Glover)的生物活性,为植物源农药开发利用提供新途径。【方法】本研究采用喷雾法施药,测定13种植物甲醇提取物对瓜蚜的室内杀虫活性与趋避活性;将具有良好活性的黄连木(P.chinensis)和川牛膝(C.officinalis)植物提取物制备成乳油,通过田间试验测定制剂防效。【结果】在杀虫活性测定中,处理72 h后,黄连木甲醇提取物对瓜蚜的活性最高,校正死亡率达到了81.11%;在对瓜蚜趋避活性测定中,毛泡桐(P.tomentosa)甲醇提取物对瓜蚜24 h的驱避率达到75.14%,薄荷(M.pulegium)、糙苏(P.umbrosa)和川牛膝甲醇提取物对瓜蚜的趋避率均高于50%;在不同植物甲醇提取物混配活性测定试验中,黄连木甲醇提取物与川牛膝、糙苏、毛泡桐、短序荚蒾(Viburnum brevipes)甲醇提取物的混配效果优于其他组合;试验研制了20%黄连木和川牛膝甲醇提取物乳油,配方为黄连木甲醇提取物10%、川牛膝甲醇提取物10%、二甲基亚砜47%、农乳500#25%、NP-2乳化剂8%。田间试验方法测定20%黄连木川牛膝甲醇提取物乳油对瓜蚜的田间防效,以10%吡虫啉可湿性粉剂为阳性对照,试验结果表明20%黄连木川牛膝甲醇提取物制剂药后14 d防治效果达到了75.26%,与阳性对照无显著差异。【结论】黄连木、糙苏、毛泡桐和川牛膝的甲醇提取物对蚜虫具有一定的生物活性,值得进一步研究。

基于阿维菌素和呋虫胺增效组合的双载纳米微囊缓释剂对苹果黄蚜的防治效果

【目的】研发苹果黄蚜可持续防控药剂及应用技术,为苹果黄蚜可持续防控提供优化方案。【方法】采用室内毒力及田间防效评价阿维菌素与呋虫胺对苹果黄蚜的联合毒力及二者纳米微囊缓释剂型的应用效果。【结果】室内毒力测定结果表明,阿维菌素与呋虫胺在1∶2和1∶4配比下对苹果黄蚜为增效作用;田间应用结果表明,阿维菌素和呋虫胺1∶4混配组合剂量(w)为15.0~22.5 mg·kg^(-1)时对苹果黄蚜药后3~15 d防效为91.44%~99.63%,二者混配可减少单一药剂有效成分用量10%~70%。按该配比制备2.25%阿维·呋虫胺纳米微囊缓释剂,田间应用结果表明,在苹果黄蚜高发期施药两次,二次药后7~15 d对苹果黄蚜的防效为79.80%~91.65%,表现出较好的后期防效;添加助剂GY-T1602可有效改善纳米微囊缓释剂的速效性。【结论】在苹果黄蚜始发期,推荐使用2.25%阿维·呋虫胺纳米微囊缓释剂以获得较长持效期,添加助剂GY-T1602可有效提高防效;在苹果黄蚜盛发期推荐选用阿维菌素与呋虫胺1∶4混配组合以快速压低虫口密度。

七星瓢虫(Coccinella septempunctata)捕食棉蚜(Aphis gossypii)的功能反应:实验与模拟(英文)

室内研究了５个温度条件（１５、２０、２５、３０和３５±０．５℃）下，七星瓢虫（Ｃｏｃｉｎｅｌａｓｅｐｔｅｍｐｕｎｃｔａｔａ）５个捕食虫态对棉蚜（Ａｐｈｉｓｇｏｓｙｐｉ）３个体积组的功能反应。测试温度范围内，瞬时攻击率（ｓｅａｒｃｈｒａｔｅ）和处置时间（ｈａｎ－ｄｌｉｎｇｔｉｍｅ）随捕食者龄期的增长而增加；每一捕食者虫态内，搜索率随猎物体积的增加而上升，而处置率则下降；搜索率随温度增加直线上升，而处置率则呈曲线上升。田间罩笼内研究了５个捕食者虫态对棉蚜混合种群的捕食功能，其捕食率远远低于室内实验。应用复合捕食方程并结合叶面积的影响对七星瓢虫捕食棉蚜的功能进行了模拟研究，且模拟值与观察值吻合程度较好。本研究表明，虽然七星瓢虫—棉蚜系统比较复杂，可以应用本研究探讨的方法建立模拟模型，以准确预测田间棉蚜的种群动态。

Hap1型棉蚜在6种菊科植物上的适合度研究

棉蚜Aphis gossypii Glover是严重危害农作物的多食性害虫,其种群已出现明显的分化。为明确Hap1型棉蚜对早春菊科Asteraceae植物的适合度,本研究用生命表参数评价了Hap1型棉蚜在苦荬菜Ixeris polycephala、泥胡菜Hemisteptia lyrata、蒲公英Taraxacum mongolicum、茼蒿Glebionis coronaria、莴苣Lactuca sativa和续断菊Sonchus asper等6种早春菊科植物上的生长和发育情况。研究结果发现,Hap1型棉蚜能在上述6种菊科植物上成功建立种群,但生命表参数存在差异。其中Hap1型棉蚜在茼蒿、苦荬菜和泥胡菜上的产仔天数均大于8 d,平均单头单日产仔数量在2.5头以上,寿命均大于16 d。Hap1型棉蚜在莴苣和续断菊上产仔天数为6 d左右,平均单头单日产仔数量分别为3.09头和1.72头,寿命为14 d左右。取食蒲公英的Hap1型棉蚜上产仔天数为2 d,平均单头单日产仔数量1.46头,在第8天死亡率就超过60%,寿命为6.74 d。研究结果表明,在上述6种菊科植物中,苦荬菜、泥胡菜和茼蒿是Hap1棉蚜的适宜寄主,莴苣和续断菊是Hap1棉蚜的较适宜寄主,而蒲公英则不是Hap1型棉蚜的适宜寄主。本研究为进一步阐明Hap1型棉蚜寄主转移规律提供了科学依据,同时为制定棉蚜的绿色防控技术奠定了理论基础。