Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adju...Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL1, TNF-α bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured. Results: TBL could significantly inhibit the synovial fibroblast proliferation (P<0.001), and downregulate IL1, TNFα and PGE 2 productions (P<0.001); indomethacin could obviously promote the synovial fibroblast proliferation(P< 0.001 ). It significantly inhibited PGE 2 production, but further upregulated IL1 and TNFα secreted by synoviocytes (P<0.01). Conclusion: The therapeutical effect of TBL on AA might be associated with its downregulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.展开更多
文摘Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin1 (IL1), tumor necrosis factor-α (TNF-α) and prostaglandin E 2 (PGE 2) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL1, TNF-α bioactivity and PGE 2 content of supernatants of cultured synoviocytes were measured. Results: TBL could significantly inhibit the synovial fibroblast proliferation (P<0.001), and downregulate IL1, TNFα and PGE 2 productions (P<0.001); indomethacin could obviously promote the synovial fibroblast proliferation(P< 0.001 ). It significantly inhibited PGE 2 production, but further upregulated IL1 and TNFα secreted by synoviocytes (P<0.01). Conclusion: The therapeutical effect of TBL on AA might be associated with its downregulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.
