Quantitative prediction of deep orebody based on 3D visualization technology is of great significance in mineral exploration. Based on the 2D traditional quantitative predicting method, the geoanomaly theory and the m...Quantitative prediction of deep orebody based on 3D visualization technology is of great significance in mineral exploration. Based on the 2D traditional quantitative predicting method, the geoanomaly theory and the mineral exploration model idea, we constructed 3D models of the topography, strata, structure, magmatite and prospecting engineering of the study area using the commercial 3D modeling software Micromine, delineated eight prospective areas and estimated the gold resources amount with methods of Abundance Estimation and Volume Estimation. Then, we compared and counted the known ore blocks and the predicted blocks, which quantitatively explains this prediction＇s validity. The results show that Xiaoqinling gold belt in Tongguan has convincing potential for gold development and utilization and the prediction method based on 3D visualization technology proves to be effective.展开更多
In order to study the migration and transformation mechanism of Hg content and occurrence form in subsurface flow zone of gold mining area in Loess Plateau and its influence on water environment,the field in-situ infi...In order to study the migration and transformation mechanism of Hg content and occurrence form in subsurface flow zone of gold mining area in Loess Plateau and its influence on water environment,the field in-situ infiltration test and laboratory test were carried out in three typical sections of river-side loess,alluvial and proluvial strata in Tongguan gold mining area of Shaanxi Province,and the following results were obtained:(1)The source of Hg in subsurface flow zone is mainly caused by mineral processing activities;(2)the subsurface flow zone in the study area is in alkaline environment,and the residual state,iron and manganese oxidation state,strong organic state and humic acid state of mercury in loess are equally divided in dry and oxidizing environment;mercury in river alluvial or diluvial strata is mainly concentrated in silt,tailings and clayey silt soil layer,and mercury has certain stability,and the form of mercury in loess is easier to transform than the other two media;(3)under the flooding condition,most of mercury is trapped in the silt layer in the undercurrent zone where the sand and silt layers alternate with each other and the river water and groundwater are disjointed,and the migration capacity of mercury is far less than that of loess layer and alluvial layer with close hydraulic connection;(4)infiltration at the flood level accelerates the migration of pollutants to the ground;(5)the soil in the undercurrent zone is overloaded and has seriously exceeded the standard.Although the groundwater monitoring results are safe this time,relevant enterprises or departments should continue to pay attention to improving the gold extraction process,especially vigorously rectify the small workshops for illegal gold extraction and the substandard discharge of the three wastes,and intensify efforts to solve the geological environmental problems of mines left over from history.At present,the occurrence form of mercury in the undercurrent zone is relatively stable,but the water and soil layers have been polluted.The risk of disjointed groundwater pollution can not be ignored while giving priority to the treatment of loess and river alluvial landform areas with close hydraulic links.The research results will provide a scientific basis for water conservancy departments to groundwater prevention and control in water-deficient areas of the Loess Plateau.展开更多
[Objectives]To evaluate the acute toxicity and toxicokinetics of Tongguan Powder in rats,and provide references for clinical safe medication.[Methods]The classical acute toxicity test method was used,rats were given d...[Objectives]To evaluate the acute toxicity and toxicokinetics of Tongguan Powder in rats,and provide references for clinical safe medication.[Methods]The classical acute toxicity test method was used,rats were given different doses of Tongguan Powder through the mouth and nasal cavity to observe the symptoms of toxicity,and make a record of the food intake,weight changes,and death.After the medication,blood was taken from each group of rats at different time points,and the plasma levels of benzoyl aconitine(BA),benzoyl hypaconine(BH)and benzoyl mesaconine(BM)were determined by the liquid chromatography tandem-mass spectrometry(LC-MS/MS),and the toxicokinetic parameters were fitted with the aid of DAS software.[Results]Rats were given Tongguan Powder 3.75 g/kg(equivalent to 54 times the human daily dose)in the nasal cavity of rats.Rats were observed with reactions such as scratching and sneezing;rats were given Tongguan Powder LD50 and 95%confidence limit of 4.15(3.53-4.71)g/kg through the oral administration,which is equivalent to 60 times the human daily dose,rats showed slow weight gain,decreased food intake,decreased voluntary activities,prone,black stools,etc.One hour after nasal administration of Tongguan Powder,the plasma concentration of benzoyl aconitine and benzoyl hypaconine was below the lower limit of detection,and benzoyl mesaconine could not be detected at any time point;one hour after the oral administration of Tongguan Powder,the plasma concentration of the three components reached the maximum,the exposure level of benzoyl hypaconine was higher than that of benzoyl aconitine and benzoyl mesaconine;there was no gender difference in the kinetic parameters.[Conclusions]The toxicity of Tongguan Powder in nasal administration is much lower than that of intragastric administration.The target organs and mechanism of toxicity need to be further studied.展开更多
Objective:Cardiac remodeling when myocardial infarction(MI)is achieved is an established prognostic factor for function-related damage and failure of hear that happen progressively.Tongguan Capsules(TGC),i.e.,a Chines...Objective:Cardiac remodeling when myocardial infarction(MI)is achieved is an established prognostic factor for function-related damage and failure of hear that happen progressively.Tongguan Capsules(TGC),i.e.,a Chinese herbal treatment with patent has been previously demonstrated its potential benefits in cardiac function,but little is known about related mechanisms.This study sought to isolate and characterize exosomal circRNA profiles from post-MI cardiac remodeling patients in response to TGC,and further explore the possible molecular mechanisms.Methods:Exosomes were isolated from the plasma and analyzed by the detection of protein marker expression and transmission electron microscopy.This study employed DESeq2 package within Bioconductor for exploring circRNAs and determining the circRNA with differential expressions.Co-expression investigation was performed with the use of a weighted correlation framework.An investigation was conducted on the molecular framework and channels of different circRNA based on the investigation system of the and Kyoto Encyclopedia of Genes and Genomes(KEGG)Gene Ontology(GO)channels.The prediction was conducted for circRNA-miRNA interactions on the basis of frequently employed target prediction software,and the framework was built with the use of Cytoscape software.Results:In total,33084 circRNAs were detected in all chromosomes and 10065 circRNAs were identified with the use of circBase.Of them,40,207 and 258 differentially expressed circRNAs were detected between the MI group and control,MI and TGC groups,and the control and TGC groups.The differentially expressed circRNAs between the MI group and control,MI group and TGC group,and control and TGC group were significantly enriched in microtubule nucleation(BP,GO:0007020),protein binding(MF,GO:0005515),regulation of natural killer cell mediated cytotoxicity(BP,GO:0042269)corresponding to the cell cycle(hsa04110),lysine degradation(hsa00310)and lysine degradation(hsa00310)in KEGG channel investigation.Module_darkorange2 indicated the most differentiation with other modules by WGCNA investigation.This study employed a total of 14 circRNAs and 8 miRNAs which have significant interactions with each other for building the circRNA-miRNA frameworks,which indicated that has-miR-619-5p,has-miR-1268a and hasmiR-1285‐3p were under the regulation of a higher amount of circRNAs as compared with other miRNAs.Conclusion:In conclusion,different mechanisms and channels participated in the pathological processes associated with cardiac remodeling following MI.The altered circRNAs are likely to be critical to the cardioprotection of TGC through the circRNA-miRNA frameworks.展开更多
Objective：To observe the effects of Tongguan Capsule（通冠胶囊,TGC） on post-myocardial infarction ventricular remodeling and heart function in rats.Methods：A rat model of acute myocardial infarction （AMI） was est...Objective：To observe the effects of Tongguan Capsule（通冠胶囊,TGC） on post-myocardial infarction ventricular remodeling and heart function in rats.Methods：A rat model of acute myocardial infarction （AMI） was established by coronary ligation.Experimental rats were randomized to 4 groups including three model groups（Group A：captopril 5 mg/kg·day,n=7;Group B：TGC 10 g/kg·day,n=7;and Group C：placebo, n=8）,and a sham-control group（Group D：blank control,n=6）.Animals were treated for 4 weeks.The cardiac function of rats was assessed at the end of the experiment based on left ventricular ejection fraction（LVEF） and left ventricular short axis fractional shortening（LVFS） detected by colored echocardiography;meanwhile, the condition of ventricular remodeling was observed through the levels of left ventricular mass（LVM）,plasma aldosterone（ALD）,myocardial angiotensinⅡ（AngⅡ） and myocardial collagen measurements.Results：At the end of the experiment,LVEF and LVFS in Group A and B were improved significantly,while those in Group C were unchanged,the LVEF in Group A,B,C,and D was 0.57±0.46,0.61±0.08,0.36±0.55 and 0.76±0.02,respectively;and their LVFS was 0.31±0.52,0.34±0.04,0.23±0.57 and 0.45±0.03,respectively.The difference was statistically significant when comparing the two indexes in Group A and B with those in Group C and D（P〈0.05）.LVM,levels of plasma ALD and myocardial AngⅡwere lower in Group A and B than in Group C,but a comparison between Group A and B showed an insignificant difference in lowering LVM and ALD,while the lowering of AngⅡwas more significant in Group B than in Group A（754.7±18.7 pg/mL vs 952.6±17.6 pg/ mL,P〈0.05）.Morphological examination showed that in Group A and B the swollen myocardial cells had shrunk, with regularly arranged myocardial fibers and decreased collagen proliferation,but the improvements in Group B were more significant.Conclusion：TGC could markedly improve the post-infarction ventricular remodeling and cardiac function in rats,showing that the efficacy was better than or equal to that of captopril.展开更多
Background:Although percutaneous coronary intervention(PCI)had become widely employed therapeutic procedure for coronary artery disease,stent restenosis limited the benefits of this revascularization and the question ...Background:Although percutaneous coronary intervention(PCI)had become widely employed therapeutic procedure for coronary artery disease,stent restenosis limited the benefits of this revascularization and the question how to prevent such events remained unresolved.While numerous empirical observations suggested Tongguan Capsules(通冠胶囊),a patented Chinese Medicine,could decrease frequency and duration of angina pectoris attacks,evidence supporting its efficacy on restenosis remained inadequate.Objective:This trial was designed to determine whether Tongguan Capsules would reduce restenosis rate in patients after successful stent implantation.Methods:Approximately 400 patients undergoing percutaneous coronary stent deployment were enrolled and randomized to control group or Tongguan Capsules(4.5 g/d)for 3 months.All patients received standard anti-platelet,anti-coagulation and lipid-decreasing treatments,concurrently.The primary clinical endpoint was the 12-month incidence of the major adverse cardiovascular events(defined as cardiac death,myocardial infarction,and recurrence of symptoms requiring additional revascularization).The angiographic end point was restenosis rate at 6 months.Conclusion:This study would provide important evidence for the use of Tongguan Capsules in patients after stent implantation in combination with routine therapies,which may significantly reduce incidence of the restenosis so as to potentially improve the clinical outcomes.(registration number:ChiCTR-TRC-ChiCTR-IIR-17011407)展开更多
基金supported by the Tongguan County, Shaanxi Province Government commissioned project, "Digital Land" and the potentiality assessment of gold resources in Tongguan County, Shaanxi Province
文摘Quantitative prediction of deep orebody based on 3D visualization technology is of great significance in mineral exploration. Based on the 2D traditional quantitative predicting method, the geoanomaly theory and the mineral exploration model idea, we constructed 3D models of the topography, strata, structure, magmatite and prospecting engineering of the study area using the commercial 3D modeling software Micromine, delineated eight prospective areas and estimated the gold resources amount with methods of Abundance Estimation and Volume Estimation. Then, we compared and counted the known ore blocks and the predicted blocks, which quantitatively explains this prediction＇s validity. The results show that Xiaoqinling gold belt in Tongguan has convincing potential for gold development and utilization and the prediction method based on 3D visualization technology proves to be effective.
基金This study was funded by the survey projects initiated by the Ministry of Natural Resources of the People’s Republic of China(1212010741003,1212011220224,and 121201011000150022)China Geological Survey(DD20189220,DD20211317)+2 种基金the public welfare scientific research project launched by the Ministry of Natural Resources of the People’s Republic of China(201111020)the project of 2015 Natural Science Basic Research Plan of Shaanxi Province of China(2015JM4129)the project of 2016 Fundamental Research Funds for the Central Universities of China(an open-end fund)(310829161128).
文摘In order to study the migration and transformation mechanism of Hg content and occurrence form in subsurface flow zone of gold mining area in Loess Plateau and its influence on water environment,the field in-situ infiltration test and laboratory test were carried out in three typical sections of river-side loess,alluvial and proluvial strata in Tongguan gold mining area of Shaanxi Province,and the following results were obtained:(1)The source of Hg in subsurface flow zone is mainly caused by mineral processing activities;(2)the subsurface flow zone in the study area is in alkaline environment,and the residual state,iron and manganese oxidation state,strong organic state and humic acid state of mercury in loess are equally divided in dry and oxidizing environment;mercury in river alluvial or diluvial strata is mainly concentrated in silt,tailings and clayey silt soil layer,and mercury has certain stability,and the form of mercury in loess is easier to transform than the other two media;(3)under the flooding condition,most of mercury is trapped in the silt layer in the undercurrent zone where the sand and silt layers alternate with each other and the river water and groundwater are disjointed,and the migration capacity of mercury is far less than that of loess layer and alluvial layer with close hydraulic connection;(4)infiltration at the flood level accelerates the migration of pollutants to the ground;(5)the soil in the undercurrent zone is overloaded and has seriously exceeded the standard.Although the groundwater monitoring results are safe this time,relevant enterprises or departments should continue to pay attention to improving the gold extraction process,especially vigorously rectify the small workshops for illegal gold extraction and the substandard discharge of the three wastes,and intensify efforts to solve the geological environmental problems of mines left over from history.At present,the occurrence form of mercury in the undercurrent zone is relatively stable,but the water and soil layers have been polluted.The risk of disjointed groundwater pollution can not be ignored while giving priority to the treatment of loess and river alluvial landform areas with close hydraulic links.The research results will provide a scientific basis for water conservancy departments to groundwater prevention and control in water-deficient areas of the Loess Plateau.
基金Key Scientific and Technological Innovation Project of Shandong Province,China(2018CXGC1304).
文摘[Objectives]To evaluate the acute toxicity and toxicokinetics of Tongguan Powder in rats,and provide references for clinical safe medication.[Methods]The classical acute toxicity test method was used,rats were given different doses of Tongguan Powder through the mouth and nasal cavity to observe the symptoms of toxicity,and make a record of the food intake,weight changes,and death.After the medication,blood was taken from each group of rats at different time points,and the plasma levels of benzoyl aconitine(BA),benzoyl hypaconine(BH)and benzoyl mesaconine(BM)were determined by the liquid chromatography tandem-mass spectrometry(LC-MS/MS),and the toxicokinetic parameters were fitted with the aid of DAS software.[Results]Rats were given Tongguan Powder 3.75 g/kg(equivalent to 54 times the human daily dose)in the nasal cavity of rats.Rats were observed with reactions such as scratching and sneezing;rats were given Tongguan Powder LD50 and 95%confidence limit of 4.15(3.53-4.71)g/kg through the oral administration,which is equivalent to 60 times the human daily dose,rats showed slow weight gain,decreased food intake,decreased voluntary activities,prone,black stools,etc.One hour after nasal administration of Tongguan Powder,the plasma concentration of benzoyl aconitine and benzoyl hypaconine was below the lower limit of detection,and benzoyl mesaconine could not be detected at any time point;one hour after the oral administration of Tongguan Powder,the plasma concentration of the three components reached the maximum,the exposure level of benzoyl hypaconine was higher than that of benzoyl aconitine and benzoyl mesaconine;there was no gender difference in the kinetic parameters.[Conclusions]The toxicity of Tongguan Powder in nasal administration is much lower than that of intragastric administration.The target organs and mechanism of toxicity need to be further studied.
文摘Objective:Cardiac remodeling when myocardial infarction(MI)is achieved is an established prognostic factor for function-related damage and failure of hear that happen progressively.Tongguan Capsules(TGC),i.e.,a Chinese herbal treatment with patent has been previously demonstrated its potential benefits in cardiac function,but little is known about related mechanisms.This study sought to isolate and characterize exosomal circRNA profiles from post-MI cardiac remodeling patients in response to TGC,and further explore the possible molecular mechanisms.Methods:Exosomes were isolated from the plasma and analyzed by the detection of protein marker expression and transmission electron microscopy.This study employed DESeq2 package within Bioconductor for exploring circRNAs and determining the circRNA with differential expressions.Co-expression investigation was performed with the use of a weighted correlation framework.An investigation was conducted on the molecular framework and channels of different circRNA based on the investigation system of the and Kyoto Encyclopedia of Genes and Genomes(KEGG)Gene Ontology(GO)channels.The prediction was conducted for circRNA-miRNA interactions on the basis of frequently employed target prediction software,and the framework was built with the use of Cytoscape software.Results:In total,33084 circRNAs were detected in all chromosomes and 10065 circRNAs were identified with the use of circBase.Of them,40,207 and 258 differentially expressed circRNAs were detected between the MI group and control,MI and TGC groups,and the control and TGC groups.The differentially expressed circRNAs between the MI group and control,MI group and TGC group,and control and TGC group were significantly enriched in microtubule nucleation(BP,GO:0007020),protein binding(MF,GO:0005515),regulation of natural killer cell mediated cytotoxicity(BP,GO:0042269)corresponding to the cell cycle(hsa04110),lysine degradation(hsa00310)and lysine degradation(hsa00310)in KEGG channel investigation.Module_darkorange2 indicated the most differentiation with other modules by WGCNA investigation.This study employed a total of 14 circRNAs and 8 miRNAs which have significant interactions with each other for building the circRNA-miRNA frameworks,which indicated that has-miR-619-5p,has-miR-1268a and hasmiR-1285‐3p were under the regulation of a higher amount of circRNAs as compared with other miRNAs.Conclusion:In conclusion,different mechanisms and channels participated in the pathological processes associated with cardiac remodeling following MI.The altered circRNAs are likely to be critical to the cardioprotection of TGC through the circRNA-miRNA frameworks.
文摘Objective：To observe the effects of Tongguan Capsule（通冠胶囊,TGC） on post-myocardial infarction ventricular remodeling and heart function in rats.Methods：A rat model of acute myocardial infarction （AMI） was established by coronary ligation.Experimental rats were randomized to 4 groups including three model groups（Group A：captopril 5 mg/kg·day,n=7;Group B：TGC 10 g/kg·day,n=7;and Group C：placebo, n=8）,and a sham-control group（Group D：blank control,n=6）.Animals were treated for 4 weeks.The cardiac function of rats was assessed at the end of the experiment based on left ventricular ejection fraction（LVEF） and left ventricular short axis fractional shortening（LVFS） detected by colored echocardiography;meanwhile, the condition of ventricular remodeling was observed through the levels of left ventricular mass（LVM）,plasma aldosterone（ALD）,myocardial angiotensinⅡ（AngⅡ） and myocardial collagen measurements.Results：At the end of the experiment,LVEF and LVFS in Group A and B were improved significantly,while those in Group C were unchanged,the LVEF in Group A,B,C,and D was 0.57±0.46,0.61±0.08,0.36±0.55 and 0.76±0.02,respectively;and their LVFS was 0.31±0.52,0.34±0.04,0.23±0.57 and 0.45±0.03,respectively.The difference was statistically significant when comparing the two indexes in Group A and B with those in Group C and D（P〈0.05）.LVM,levels of plasma ALD and myocardial AngⅡwere lower in Group A and B than in Group C,but a comparison between Group A and B showed an insignificant difference in lowering LVM and ALD,while the lowering of AngⅡwas more significant in Group B than in Group A（754.7±18.7 pg/mL vs 952.6±17.6 pg/ mL,P〈0.05）.Morphological examination showed that in Group A and B the swollen myocardial cells had shrunk, with regularly arranged myocardial fibers and decreased collagen proliferation,but the improvements in Group B were more significant.Conclusion：TGC could markedly improve the post-infarction ventricular remodeling and cardiac function in rats,showing that the efficacy was better than or equal to that of captopril.
基金Supported by the National Natural Science Foundation of China(No.81703848 and No.81703877)the Natural Science Foundation of Guandong Province(No.2017A030310123)+1 种基金CHEN Ke-ji Academic Thought Inheritance Studio(No.201461)SHI Zai-xiang Academic Thought Inheritance Studio(No.201461)。
文摘Background:Although percutaneous coronary intervention(PCI)had become widely employed therapeutic procedure for coronary artery disease,stent restenosis limited the benefits of this revascularization and the question how to prevent such events remained unresolved.While numerous empirical observations suggested Tongguan Capsules(通冠胶囊),a patented Chinese Medicine,could decrease frequency and duration of angina pectoris attacks,evidence supporting its efficacy on restenosis remained inadequate.Objective:This trial was designed to determine whether Tongguan Capsules would reduce restenosis rate in patients after successful stent implantation.Methods:Approximately 400 patients undergoing percutaneous coronary stent deployment were enrolled and randomized to control group or Tongguan Capsules(4.5 g/d)for 3 months.All patients received standard anti-platelet,anti-coagulation and lipid-decreasing treatments,concurrently.The primary clinical endpoint was the 12-month incidence of the major adverse cardiovascular events(defined as cardiac death,myocardial infarction,and recurrence of symptoms requiring additional revascularization).The angiographic end point was restenosis rate at 6 months.Conclusion:This study would provide important evidence for the use of Tongguan Capsules in patients after stent implantation in combination with routine therapies,which may significantly reduce incidence of the restenosis so as to potentially improve the clinical outcomes.(registration number:ChiCTR-TRC-ChiCTR-IIR-17011407)
基金the National Key Research and Development Program of China(2018YFC1705004)Tianjin Science and Technology Plan Project(21JCZDJC00890)National Natural Science Foundation of China(82374578).
