Stroke is one of the leading causes of death and disability worldwide.However,information on stroke-related tongue coating microbiome(TCM)is limited,and whether TCM modulation could benefit for stroke prevention and r...Stroke is one of the leading causes of death and disability worldwide.However,information on stroke-related tongue coating microbiome(TCM)is limited,and whether TCM modulation could benefit for stroke prevention and rehabilitation is unknown.Here,TCM from stroke patients(SP)was characterized using molecular techniques.The occurrence of stroke resulted in TCM dysbiosis with significantly reduced species richness and diversity.The abundance of Prevotella,Leptotrichia,Actinomyces,Alloprevotella,Haemophilus,and TM7_[G-1]were greatly reduced,but common infection Streptococcus and Pseudomonas were remarkably increased.Furthermore,an antioxidative probiotic Lactiplantibacillus plantarum AR113 was used for TCM intervention in stroke rats with cerebral ischemia/reperfusion(I/R).AR113 partly restored I/R induced change of TCM and gut microbiota with significantly improved neurological deficit,relieved histopathologic change,increased activities of antioxidant enzymes,and decreased contents of oxidative stress biomarkers.Moreover,the gene expression of antioxidant-related proteins and apoptosis-related factors heme oxygenase-1(HO-1),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),nuclear factor erythroid 2-related factor 2(Nrf2),NAD(P)H:quinone oxidoreductase-1(NQO-1),and Bcl-2 was significantly increased,but cytochrome C,cleaved caspase-3,and Bax were markedly decreased in the brain by AR113 treatment.The results suggested that AR113 could ameliorate cerebral I/R injury through antioxidation and anti-apoptosis pathways,and AR113 intervention of TCM may have the application potential for stroke prevention and control.展开更多
International Organization for Standardization(ISO)officially released ISO/TR 20498-5 Traditional Chinese medicine-Computerized tongue image analysis system--Part 5:Method of acquisition and expression of tongue colou...International Organization for Standardization(ISO)officially released ISO/TR 20498-5 Traditional Chinese medicine-Computerized tongue image analysis system--Part 5:Method of acquisition and expression of tongue colour and tongue coating colour on January 11,2019.ISO/TR 20498-5 was formulated by the team of Professor Wang Yiqin,from School of Basic Medicine of Shanghai University of TCM and Shanghai Key Laboratory of Health Identification and Evaluation,and jointly completed by East China University of Science and Technology and Suzhou Jingyu Medical Instrument Co.,LTD.展开更多
<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atr...<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atrophic gastritis patients and 29 age and gender-matched non-atrophic gastritis controls. By sequencing the V3-V4 region of the 16S rRNA, we investigated the microbial community structure and diversity on the tongue coating. <b>Results:</b> There was no significant difference in the microbial diversity on the tongue coating between the two groups. However, compared with the control, the atrophic gastritis group had a smaller number of operational taxonomic units (OTUs). At the class level, patients with atrophic gastritis had lower relative abundances of <i>Betaproteobacteria</i> and <i>Spirochaetia</i> than the control group. At the gene level, the abundance of <i>Neisseria</i> and <i>Aggregatibacter</i> in atrophic gastritis group had significantly decreased compared with control ones. Furthermore, functional prediction revealed that 24 metabolic pathways significantly differed between the two groups. <b>Conclusions:</b> Our findings provide novel evidence that tongue coating microbiota may be a biomarker for characterizing patient with atrophic gastritis, but its mechanism needs to be further elaborated.展开更多
Observation of tongue coating is the characteristic content of traditional Chinese medicine syndrome differentiation and treatment. The microflora is the important component of tongue coating besides the desquamated e...Observation of tongue coating is the characteristic content of traditional Chinese medicine syndrome differentiation and treatment. The microflora is the important component of tongue coating besides the desquamated epithelial cells. Tongue coating microflora might be an important linkage between the modern medical research and the classic traditional Chinese medicine theory. Therefore, the present paper summarised the application value of tongue coating microflora in health and disease to enrich the scienti fic connotation of traditional Chinese medicine tongue diagnosis and promote the development of integration of traditional Chinese medicine and modern medicine.展开更多
Background:According to World Health Organization,colorectal cancer is the third most common cancer in the world.The prognosis assessment and condition judgment of the colorectal cancer remains a challenge clinically....Background:According to World Health Organization,colorectal cancer is the third most common cancer in the world.The prognosis assessment and condition judgment of the colorectal cancer remains a challenge clinically.Therefore,identification of diagnostic markers to evaluate the prognosis of colorectal cancer clinically should be urgently developed.We have observed that a lot of cancer patients had thick tongue clinically,but what is the relationship between tongue coating and the tumor?Methods:Seventy-four patients with colorectal carcinoma were collected through the outpatients of Tianjin Medical University Cancer Institute and Hospital from May 2010 to September 2011,in which there were 49 patients confirmed with recurrence or metastasis.All photos of patients’tongue were taken with a SONY camera in the same room and under constant conditions such as brightness or distance.Regression equation predicting thickness of tongue coating was constructed using binary logistic regression analysis.The optimal cut off of probabilities to diagnosis thick tongue coating was determined by receiver operating curve analysis.χ2 test for paired data and kappa test were used to determine the diagnostic value for recurrence and/or metastasis in colorectal cancer patients.Kolmogorov-Smirnov test was used to determine the distribution of ALT,AST,ALP ALB,TP,GLO,TBIL,DBIL,GGT,LDH,GLU,UA,CA724,CA199,CA242 and CEA.Data with the skewed distribution were presented as median(quartile interval).The association between the thickness of tongue coating and clinical-pathological character was evaluated by chi square test and two-independent-sample test.The two-independent-samplesχ2 was used to determine whether there were significant differences in the thin coating and thick coating between patients with recurrence and/or metastasis and patients without recurrence and/or metastasis.Kaplan-Meier method was used to analyze survival time.Statistical analysis was performed by SPSS(version 16.0).Results:Through retrospective clinical study we found that overall survival of colorectal cancer patients with thick greasy tongue coating is less than the patients with less tongue coating.What’s more,the risk for recurrence or/and metastasis overall survival in thick coating group was higher than thin coating group.In addition,the histological staining of the tongue slices of rats showed that EGFR receptors in the tongue root were the most among whole tongue surface.Tongue thick coating may be due to tumor patients with high levels of serum EGF which results in significantly increasing tongue coating.This finding suggested that the tongue coating of cancer patients may reflect the level of serum EGF levels in patients which may be related to shorter survival time.In addition,another study showed that serum lactic dehydrogenase level in patients with thick tongue coating is higher than patients with thin tongue coating.Conclusions:These studies suggest that tongue coating is likely to reflect some of the growth factor and enzyme levels.By observing the tongue coating we could predict the prognosis of patients and the characteristics of tongue coating may be used as new diagnostic markers to patients with colorectal carcinoma.展开更多
Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of s...Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of stained tongue coating from healthy students at Hunan University of Chinese Medicine and 1007 images of pathological(non-stained)tongue coat-ing from hospitalized patients at The First Hospital of Hunan University of Chinese Medicine withlungcancer;diabetes;andhypertensionwerecollected.Thetongueimageswererandomi-zed into the training;validation;and testing datasets in a 7:2:1 ratio.A deep learning model was constructed using the ResNet50 for recognizing stained tongue coating in the training and validation datasets.The training period was 90 epochs.The model’s performance was evaluated by its accuracy;loss curve;recall;F1 score;confusion matrix;receiver operating characteristic(ROC)curve;and precision-recall(PR)curve in the tasks of predicting stained tongue coating images in the testing dataset.The accuracy of the deep learning model was compared with that of attending physicians of traditional Chinese medicine(TCM).Results The training results showed that after 90 epochs;the model presented an excellent classification performance.The loss curve and accuracy were stable;showing no signs of overfitting.The model achieved an accuracy;recall;and F1 score of 92%;91%;and 92%;re-spectively.The confusion matrix revealed an accuracy of 92%for the model and 69%for TCM practitioners.The areas under the ROC and PR curves were 0.97 and 0.95;respectively.Conclusion The deep learning model constructed using ResNet50 can effectively recognize stained coating images with greater accuracy than visual inspection of TCM practitioners.This model has the potential to assist doctors in identifying false tongue coating and prevent-ing misdiagnosis.展开更多
Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stab...Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.展开更多
Cavitation erosion (CE) is the predominant cause for the failure of overflow components in fluid machinery. Advanced coatings have provided an effective solution to cavitation erosion due to the rapid development of...Cavitation erosion (CE) is the predominant cause for the failure of overflow components in fluid machinery. Advanced coatings have provided an effective solution to cavitation erosion due to the rapid development of surface engineering techniques. However, the influence of coating structures on CE resistance has not been sys- tematically studied. To better understand their relationship, micro-nano and conventional WC-10Co4Cr cermet coat- ings are deposited by high velocity oxygen fuel spray- ing(HVOF), and their microstructures are analyzed by OM, SEM and XRD. Meanwhile, characterizations of mechan- ical and electrochemical properties of the coatings are carried out, as well as the coatings' resistance to CE in 3.5 wt % NaC1 solution, and the cavitation mechanisms are explored. Results show that micro-nano WC-10Co4Cr coating possesses dense microstructure, excellent mechanical and electrochemical properties, with very low porosity of 0.26 4-0.07% and extraordinary fracture toughness of 5.58 4-0.51 MPa.m1/2. Moreover, the CE resistance of micro-nano coating is enhanced above 50% than conventional coating at the steady CE period in 3.5 wt % NaC1 solution. The superior CE resistance of micro- nano WC-10Co4Cr coating may originate from the unique micro-nano structure and properties, which can effectively obstruct the formation and propagation of CE crack. Thus,a new method is proposed to enhance the CE resistance of WC-10Co4Cr coating by manipulating the microstructure.展开更多
An organic-inorganic hybrid sealing agent was fabricated and used in the plasma sprayed Al_(2)O_(3)-13 wt%TiO_(2)coating,and conventional silicone agent was also used for comparison.Protection performance of the coati...An organic-inorganic hybrid sealing agent was fabricated and used in the plasma sprayed Al_(2)O_(3)-13 wt%TiO_(2)coating,and conventional silicone agent was also used for comparison.Protection performance of the coatings was comprehensively evaluated based on both anti-corrosion and anti-biofouling properties.The results reveal that the sealing treatment is remarkably useful to decrease the porosity of the coating,and the porosity of the coating sealed with the hybrid agent is only 0.035%.Immersion corrosion test and Tafel polarization test reveal that the sealed coating with the hybrid agent exhibits a better corrosion resistance by compared with the coating sealed with silicone agent.The corrosion current density i_(corr) of the hybrid agent sealed coating is only 0.7×10^(-6)A·cm^(-2).Moreover,anti-biofouling tests both in the outdoor analogue hydraulic environment and in the natural marine environment prove that the mentioned novel coating presents a better combination of corrosion resistance and anti-biofouling property by compared with the other coatings,and it could be used as a protection of metal components in the marine environment.展开更多
The relation between structures and properties of polyurethane are investigated by modern physical and chemical methods.The results obtained are as follows:the effects of the content of self-crosslinking agent on the ...The relation between structures and properties of polyurethane are investigated by modern physical and chemical methods.The results obtained are as follows:the effects of the content of self-crosslinking agent on the properties of polyurethane,i.e.,dispersion stability,dynamical viscoelasticity and mechanical properties are discussed.It is found that the optimum molar ratio of epichlorohydrin and diethylenetriamine is 1:2.A mois-展开更多
To improve the durability of underwater rotating products,the corrosion characteristics in harsh marine environment were evaluated through various electrochemical experiments on the Al2O3-3TiO2 and CoNiCrAlY coating l...To improve the durability of underwater rotating products,the corrosion characteristics in harsh marine environment were evaluated through various electrochemical experiments on the Al2O3-3TiO2 and CoNiCrAlY coating layers by atmospheric pressure plasma spray coating process.By evaluating the corrosion resistance of these materials,their applicability to environmentally friendly power generation equipment such as blades of tidal current turbines was examined.According to the Tafel analysis for micro-areas including the coating layer,the coating/metal interlayer and the base metal,the Al2O3-3TiO2 coating layer and the CoNiCrAlY coating layer show markedly lower corrosion current density than the base metal.The corrosion current density of the CoNiCrAlY coating layer (9.75316×10-8A/cm2) is about 1.6 times more than that of the Al2O3-3TiO2 coating layer (6.13139×10-8A/cm2).展开更多
Pure W and W-Cu-W trilayer coatings were deposited on an Fe substrate by d.c. magnetron sputtering. The α-β phase evolution, intragranular stress evolution in sputter-deposited W layer were investigated by x-ray dif...Pure W and W-Cu-W trilayer coatings were deposited on an Fe substrate by d.c. magnetron sputtering. The α-β phase evolution, intragranular stress evolution in sputter-deposited W layer were investigated by x-ray diffraction. They are directly related to the film microstructure, density and adhesion. Therefore, control of the film stress and phase component transition is essential for its applications. The phase component transition from β-W to α-W and intragranular stress evolution from tensile to compressive strongly depend on the deposition parameters and can be induced by lowering Ar pressure and rising target power. The compressively stressed films with α-W phase have a dense microstructure and high adhesion to Fe substrate.展开更多
In this study, two thermal barrier coatings based on YSZ were produced by using a commercially available agglomerated and sintered powder and a special spray powder prepared by high energy ball milling. Both thermal b...In this study, two thermal barrier coatings based on YSZ were produced by using a commercially available agglomerated and sintered powder and a special spray powder prepared by high energy ball milling. Both thermal barrier coatings exhibited similar overall porosities, but significantly different microstructures. Application of the special spray powder prepared by high energy ball milling led to a microstructure with numerous inclusions of semi-molten agglomerates, which introduced a plethora of clusters of fine pores into the coating and several more microstructural defects. This microstructure resulted in a significantly better thermal shock behavior compared to the conventional thermal barrier coating. The heat treatment of both thermal barrier coatings atθ=1150℃for t=100 h led to a sintering of both coatings. The results were reduced overall porosity and significantly increased fracture toughness. A correlation between the fracture toughness of both coatings after the heat treatment and the thermal shock life time could not be identified.展开更多
1 Scope This standard specifies the term, definition, classification, technical requirements, test methods, quality appraisal procedures, packing, marking, transportation, storage, and quality certificate of coating r...1 Scope This standard specifies the term, definition, classification, technical requirements, test methods, quality appraisal procedures, packing, marking, transportation, storage, and quality certificate of coating resistant refractory eastables.展开更多
Fatigue crack growth tests were carried out on the SEN B3 precracked specimens, with dimensions in accordance with ISO 12108 requirements. The specimens were made of martensitic stainless steel, X17CrNi15-2, and some ...Fatigue crack growth tests were carried out on the SEN B3 precracked specimens, with dimensions in accordance with ISO 12108 requirements. The specimens were made of martensitic stainless steel, X17CrNi15-2, and some of them were modified by the ceramic coating deposition surface treatment. The effects of ceramic coating, on the fatigue crack growth behaviour of hollow shaft specimens, were experimentally investigated. Fatigue crack growth rates, da/dN, were characterised, using the power law relationship between da/dN (in mm/cycle) and the stress intensity factor range, ΔK (in MPa∙m0.5). The two constants of the correlation are 7.9768 × 10−9 and 2.8107 for the parent material, and those for the coated material are 2.4391 × 10−9 and 3.1990, respectively. Microstructural analyses were carried out on the tested specimens, which shows that the maximum hardness of the ceramic coating is higher than that of substrate by a factor of ~3.2. The dimple fracture dominates the final fracture mechanism for the parent material, and the combination of fatigue, ductile fracture and cleavage dominates the final fracture mechanism for the coated material, based on the SEM analyses. EDS tests’ results reveal that the parent material specimen shows higher levels of C at matrix regions along with Fe- and O-rich regions, compared with the coated material specimen.展开更多
The conventional Al2O3-13 wt. % TiO2 composite ceramic coatings are fabricated by plasma spraying on the surface of extrusion wheel. The microstrueture, morphology and phase compositions of the substrate and coat- ing...The conventional Al2O3-13 wt. % TiO2 composite ceramic coatings are fabricated by plasma spraying on the surface of extrusion wheel. The microstrueture, morphology and phase compositions of the substrate and coat- ing are investigated by using X-ray diffractometry (XRD) , scanning electron microsopy (SEM) and energy dis- persive spectroscopy (EDS). Moreover, the microhardness of the substrate and the coating are investigated using Vickers mierohardness tester, the friction and wear behaviors of the substrate and the coating are investigated by using a block-on-ring tribometer under dry sliding conditions with the load of 245 N. The results show that both γ-Al2O3 and α-Al2O3 phases are observed in the as-sprayed coatings, the mian phase is γ-Al2O3. There are white particulates Al2O3 on its surface. The Al2O3-13 wt. % TiO2 coating possesses higher mierohardness which is about 1018HV and 1.6 times that of the substrate. The wear performance of coating is better than that of the substrate. In a practical application, the life of the extrusion wheel which is plasma sprayed Al2O3-13 wt. % TiO2 coating on the surface is 1.2 times that of the conventional extrusion wheel, and the life is about 330 h.展开更多
A novelγ-γ'(Ni solid solution—Ni_(3)Al)NiPtAl coating was produced on advanced single-crystal(SC)superalloy N5 by electroplating of a thin Pt coating with~3μm in thickness followed by heat treatment at 1000℃i...A novelγ-γ'(Ni solid solution—Ni_(3)Al)NiPtAl coating was produced on advanced single-crystal(SC)superalloy N5 by electroplating of a thin Pt coating with~3μm in thickness followed by heat treatment at 1000℃in vacuum.For comparison,a traditionalγ-γ'NiPtAl coating was also produced by electroplating of a thick Pt coating with~6μm in thickness followed by heat treatment at 1100℃in vacuum.The novel coating has a mass gain of~0.5 mg·cm^(-2)after 100 h 1-h cyclic oxidation at 1150℃,showing better oxidation resistance than the traditional coating.An oxide scale mostly consisting ofα-Al_(2)O_(3)grew on the novel coating because the element Cr from the SC alloy substrate contributes to the formation ofα-Al_(2)O_(3).Pt content in the novel coating has significant effect on the oxidation resistance.The optimized Pt content in the coating is about 16 at%,which is helpful to reduce the diffusion of harmful elements from the substrate into the coating but also to reduce the cost of the coating.展开更多
It is significant for low-cost preparation of YBa2Cu3O7-δ(YBCO) coated conductors to make clear the mechanism of orientation, copper segregation, and nucleation density in BaF2-derived YBCO crystallization. In the ...It is significant for low-cost preparation of YBa2Cu3O7-δ(YBCO) coated conductors to make clear the mechanism of orientation, copper segregation, and nucleation density in BaF2-derived YBCO crystallization. In the present work,a distinct nucleation mechanism was proposed based on a transient liquid phase induced by the size effect as well as near-equilibrium assumption. With this scheme the nucleation of YBCO prepared by metal–organic deposition(MOD) or the physical vapor deposition BaF2 process was semi-quantitatively analyzed, revealing that the direct driving force for nucleation is YBCO supersaturation in the liquid phase. The theoretical analysis on the nucleation orientation portion is evidenced by the experimental result.展开更多
基金supported by National Science Fund for Distinguished Young Scholars(grant No.32025029)Shanghai Education Committee Scientific Research Innovation Project(grant No.2101070007800120)+1 种基金Clinical research project in health industry of Shanghai Municipal Health Commission(202240379)the Development Fund for Shanghai Talents(grant No.2021077).
文摘Stroke is one of the leading causes of death and disability worldwide.However,information on stroke-related tongue coating microbiome(TCM)is limited,and whether TCM modulation could benefit for stroke prevention and rehabilitation is unknown.Here,TCM from stroke patients(SP)was characterized using molecular techniques.The occurrence of stroke resulted in TCM dysbiosis with significantly reduced species richness and diversity.The abundance of Prevotella,Leptotrichia,Actinomyces,Alloprevotella,Haemophilus,and TM7_[G-1]were greatly reduced,but common infection Streptococcus and Pseudomonas were remarkably increased.Furthermore,an antioxidative probiotic Lactiplantibacillus plantarum AR113 was used for TCM intervention in stroke rats with cerebral ischemia/reperfusion(I/R).AR113 partly restored I/R induced change of TCM and gut microbiota with significantly improved neurological deficit,relieved histopathologic change,increased activities of antioxidant enzymes,and decreased contents of oxidative stress biomarkers.Moreover,the gene expression of antioxidant-related proteins and apoptosis-related factors heme oxygenase-1(HO-1),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),nuclear factor erythroid 2-related factor 2(Nrf2),NAD(P)H:quinone oxidoreductase-1(NQO-1),and Bcl-2 was significantly increased,but cytochrome C,cleaved caspase-3,and Bax were markedly decreased in the brain by AR113 treatment.The results suggested that AR113 could ameliorate cerebral I/R injury through antioxidation and anti-apoptosis pathways,and AR113 intervention of TCM may have the application potential for stroke prevention and control.
文摘International Organization for Standardization(ISO)officially released ISO/TR 20498-5 Traditional Chinese medicine-Computerized tongue image analysis system--Part 5:Method of acquisition and expression of tongue colour and tongue coating colour on January 11,2019.ISO/TR 20498-5 was formulated by the team of Professor Wang Yiqin,from School of Basic Medicine of Shanghai University of TCM and Shanghai Key Laboratory of Health Identification and Evaluation,and jointly completed by East China University of Science and Technology and Suzhou Jingyu Medical Instrument Co.,LTD.
文摘<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atrophic gastritis patients and 29 age and gender-matched non-atrophic gastritis controls. By sequencing the V3-V4 region of the 16S rRNA, we investigated the microbial community structure and diversity on the tongue coating. <b>Results:</b> There was no significant difference in the microbial diversity on the tongue coating between the two groups. However, compared with the control, the atrophic gastritis group had a smaller number of operational taxonomic units (OTUs). At the class level, patients with atrophic gastritis had lower relative abundances of <i>Betaproteobacteria</i> and <i>Spirochaetia</i> than the control group. At the gene level, the abundance of <i>Neisseria</i> and <i>Aggregatibacter</i> in atrophic gastritis group had significantly decreased compared with control ones. Furthermore, functional prediction revealed that 24 metabolic pathways significantly differed between the two groups. <b>Conclusions:</b> Our findings provide novel evidence that tongue coating microbiota may be a biomarker for characterizing patient with atrophic gastritis, but its mechanism needs to be further elaborated.
基金National Natural Science Foundation (81473593, 81473458).
文摘Observation of tongue coating is the characteristic content of traditional Chinese medicine syndrome differentiation and treatment. The microflora is the important component of tongue coating besides the desquamated epithelial cells. Tongue coating microflora might be an important linkage between the modern medical research and the classic traditional Chinese medicine theory. Therefore, the present paper summarised the application value of tongue coating microflora in health and disease to enrich the scienti fic connotation of traditional Chinese medicine tongue diagnosis and promote the development of integration of traditional Chinese medicine and modern medicine.
文摘Background:According to World Health Organization,colorectal cancer is the third most common cancer in the world.The prognosis assessment and condition judgment of the colorectal cancer remains a challenge clinically.Therefore,identification of diagnostic markers to evaluate the prognosis of colorectal cancer clinically should be urgently developed.We have observed that a lot of cancer patients had thick tongue clinically,but what is the relationship between tongue coating and the tumor?Methods:Seventy-four patients with colorectal carcinoma were collected through the outpatients of Tianjin Medical University Cancer Institute and Hospital from May 2010 to September 2011,in which there were 49 patients confirmed with recurrence or metastasis.All photos of patients’tongue were taken with a SONY camera in the same room and under constant conditions such as brightness or distance.Regression equation predicting thickness of tongue coating was constructed using binary logistic regression analysis.The optimal cut off of probabilities to diagnosis thick tongue coating was determined by receiver operating curve analysis.χ2 test for paired data and kappa test were used to determine the diagnostic value for recurrence and/or metastasis in colorectal cancer patients.Kolmogorov-Smirnov test was used to determine the distribution of ALT,AST,ALP ALB,TP,GLO,TBIL,DBIL,GGT,LDH,GLU,UA,CA724,CA199,CA242 and CEA.Data with the skewed distribution were presented as median(quartile interval).The association between the thickness of tongue coating and clinical-pathological character was evaluated by chi square test and two-independent-sample test.The two-independent-samplesχ2 was used to determine whether there were significant differences in the thin coating and thick coating between patients with recurrence and/or metastasis and patients without recurrence and/or metastasis.Kaplan-Meier method was used to analyze survival time.Statistical analysis was performed by SPSS(version 16.0).Results:Through retrospective clinical study we found that overall survival of colorectal cancer patients with thick greasy tongue coating is less than the patients with less tongue coating.What’s more,the risk for recurrence or/and metastasis overall survival in thick coating group was higher than thin coating group.In addition,the histological staining of the tongue slices of rats showed that EGFR receptors in the tongue root were the most among whole tongue surface.Tongue thick coating may be due to tumor patients with high levels of serum EGF which results in significantly increasing tongue coating.This finding suggested that the tongue coating of cancer patients may reflect the level of serum EGF levels in patients which may be related to shorter survival time.In addition,another study showed that serum lactic dehydrogenase level in patients with thick tongue coating is higher than patients with thin tongue coating.Conclusions:These studies suggest that tongue coating is likely to reflect some of the growth factor and enzyme levels.By observing the tongue coating we could predict the prognosis of patients and the characteristics of tongue coating may be used as new diagnostic markers to patients with colorectal carcinoma.
基金National Natural Science Foundation of China(82274411)Science and Technology Innovation Program of Hunan Province(2022RC1021)Leading Research Project of Hunan University of Chinese Medicine(2022XJJB002).
文摘Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of stained tongue coating from healthy students at Hunan University of Chinese Medicine and 1007 images of pathological(non-stained)tongue coat-ing from hospitalized patients at The First Hospital of Hunan University of Chinese Medicine withlungcancer;diabetes;andhypertensionwerecollected.Thetongueimageswererandomi-zed into the training;validation;and testing datasets in a 7:2:1 ratio.A deep learning model was constructed using the ResNet50 for recognizing stained tongue coating in the training and validation datasets.The training period was 90 epochs.The model’s performance was evaluated by its accuracy;loss curve;recall;F1 score;confusion matrix;receiver operating characteristic(ROC)curve;and precision-recall(PR)curve in the tasks of predicting stained tongue coating images in the testing dataset.The accuracy of the deep learning model was compared with that of attending physicians of traditional Chinese medicine(TCM).Results The training results showed that after 90 epochs;the model presented an excellent classification performance.The loss curve and accuracy were stable;showing no signs of overfitting.The model achieved an accuracy;recall;and F1 score of 92%;91%;and 92%;re-spectively.The confusion matrix revealed an accuracy of 92%for the model and 69%for TCM practitioners.The areas under the ROC and PR curves were 0.97 and 0.95;respectively.Conclusion The deep learning model constructed using ResNet50 can effectively recognize stained coating images with greater accuracy than visual inspection of TCM practitioners.This model has the potential to assist doctors in identifying false tongue coating and prevent-ing misdiagnosis.
基金Supported by Key Disciplines Group Construetion Project of Pudong Health Bureau of Shanghai(Grant No.PWZxk2010-12)
文摘Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.
基金Supported by National Natural Science Foundation of China (Grand No. 51422507)
文摘Cavitation erosion (CE) is the predominant cause for the failure of overflow components in fluid machinery. Advanced coatings have provided an effective solution to cavitation erosion due to the rapid development of surface engineering techniques. However, the influence of coating structures on CE resistance has not been sys- tematically studied. To better understand their relationship, micro-nano and conventional WC-10Co4Cr cermet coat- ings are deposited by high velocity oxygen fuel spray- ing(HVOF), and their microstructures are analyzed by OM, SEM and XRD. Meanwhile, characterizations of mechan- ical and electrochemical properties of the coatings are carried out, as well as the coatings' resistance to CE in 3.5 wt % NaC1 solution, and the cavitation mechanisms are explored. Results show that micro-nano WC-10Co4Cr coating possesses dense microstructure, excellent mechanical and electrochemical properties, with very low porosity of 0.26 4-0.07% and extraordinary fracture toughness of 5.58 4-0.51 MPa.m1/2. Moreover, the CE resistance of micro-nano coating is enhanced above 50% than conventional coating at the steady CE period in 3.5 wt % NaC1 solution. The superior CE resistance of micro- nano WC-10Co4Cr coating may originate from the unique micro-nano structure and properties, which can effectively obstruct the formation and propagation of CE crack. Thus,a new method is proposed to enhance the CE resistance of WC-10Co4Cr coating by manipulating the microstructure.
基金the National Natural Science Foundation of China(No.51379070)the Graduate Research and Innovation Projects of Jiangsu Province(No.KYCX21_0463)。
文摘An organic-inorganic hybrid sealing agent was fabricated and used in the plasma sprayed Al_(2)O_(3)-13 wt%TiO_(2)coating,and conventional silicone agent was also used for comparison.Protection performance of the coatings was comprehensively evaluated based on both anti-corrosion and anti-biofouling properties.The results reveal that the sealing treatment is remarkably useful to decrease the porosity of the coating,and the porosity of the coating sealed with the hybrid agent is only 0.035%.Immersion corrosion test and Tafel polarization test reveal that the sealed coating with the hybrid agent exhibits a better corrosion resistance by compared with the coating sealed with silicone agent.The corrosion current density i_(corr) of the hybrid agent sealed coating is only 0.7×10^(-6)A·cm^(-2).Moreover,anti-biofouling tests both in the outdoor analogue hydraulic environment and in the natural marine environment prove that the mentioned novel coating presents a better combination of corrosion resistance and anti-biofouling property by compared with the other coatings,and it could be used as a protection of metal components in the marine environment.
文摘The relation between structures and properties of polyurethane are investigated by modern physical and chemical methods.The results obtained are as follows:the effects of the content of self-crosslinking agent on the properties of polyurethane,i.e.,dispersion stability,dynamical viscoelasticity and mechanical properties are discussed.It is found that the optimum molar ratio of epichlorohydrin and diethylenetriamine is 1:2.A mois-
基金Project supported by the Ministry of Education,Science Technology(MEST)Korea Industrial Technology Foundation(KOTEF)through the Human Resource Training Project for Regional Innovation
文摘To improve the durability of underwater rotating products,the corrosion characteristics in harsh marine environment were evaluated through various electrochemical experiments on the Al2O3-3TiO2 and CoNiCrAlY coating layers by atmospheric pressure plasma spray coating process.By evaluating the corrosion resistance of these materials,their applicability to environmentally friendly power generation equipment such as blades of tidal current turbines was examined.According to the Tafel analysis for micro-areas including the coating layer,the coating/metal interlayer and the base metal,the Al2O3-3TiO2 coating layer and the CoNiCrAlY coating layer show markedly lower corrosion current density than the base metal.The corrosion current density of the CoNiCrAlY coating layer (9.75316×10-8A/cm2) is about 1.6 times more than that of the Al2O3-3TiO2 coating layer (6.13139×10-8A/cm2).
文摘Pure W and W-Cu-W trilayer coatings were deposited on an Fe substrate by d.c. magnetron sputtering. The α-β phase evolution, intragranular stress evolution in sputter-deposited W layer were investigated by x-ray diffraction. They are directly related to the film microstructure, density and adhesion. Therefore, control of the film stress and phase component transition is essential for its applications. The phase component transition from β-W to α-W and intragranular stress evolution from tensile to compressive strongly depend on the deposition parameters and can be induced by lowering Ar pressure and rising target power. The compressively stressed films with α-W phase have a dense microstructure and high adhesion to Fe substrate.
基金the German Science Foundation (DFG) for financially supporting the research work within the scope of the DFG projects ZH205/2-1 and BO1979/32-2
文摘In this study, two thermal barrier coatings based on YSZ were produced by using a commercially available agglomerated and sintered powder and a special spray powder prepared by high energy ball milling. Both thermal barrier coatings exhibited similar overall porosities, but significantly different microstructures. Application of the special spray powder prepared by high energy ball milling led to a microstructure with numerous inclusions of semi-molten agglomerates, which introduced a plethora of clusters of fine pores into the coating and several more microstructural defects. This microstructure resulted in a significantly better thermal shock behavior compared to the conventional thermal barrier coating. The heat treatment of both thermal barrier coatings atθ=1150℃for t=100 h led to a sintering of both coatings. The results were reduced overall porosity and significantly increased fracture toughness. A correlation between the fracture toughness of both coatings after the heat treatment and the thermal shock life time could not be identified.
文摘1 Scope This standard specifies the term, definition, classification, technical requirements, test methods, quality appraisal procedures, packing, marking, transportation, storage, and quality certificate of coating resistant refractory eastables.
文摘Fatigue crack growth tests were carried out on the SEN B3 precracked specimens, with dimensions in accordance with ISO 12108 requirements. The specimens were made of martensitic stainless steel, X17CrNi15-2, and some of them were modified by the ceramic coating deposition surface treatment. The effects of ceramic coating, on the fatigue crack growth behaviour of hollow shaft specimens, were experimentally investigated. Fatigue crack growth rates, da/dN, were characterised, using the power law relationship between da/dN (in mm/cycle) and the stress intensity factor range, ΔK (in MPa∙m0.5). The two constants of the correlation are 7.9768 × 10−9 and 2.8107 for the parent material, and those for the coated material are 2.4391 × 10−9 and 3.1990, respectively. Microstructural analyses were carried out on the tested specimens, which shows that the maximum hardness of the ceramic coating is higher than that of substrate by a factor of ~3.2. The dimple fracture dominates the final fracture mechanism for the parent material, and the combination of fatigue, ductile fracture and cleavage dominates the final fracture mechanism for the coated material, based on the SEM analyses. EDS tests’ results reveal that the parent material specimen shows higher levels of C at matrix regions along with Fe- and O-rich regions, compared with the coated material specimen.
文摘The conventional Al2O3-13 wt. % TiO2 composite ceramic coatings are fabricated by plasma spraying on the surface of extrusion wheel. The microstrueture, morphology and phase compositions of the substrate and coat- ing are investigated by using X-ray diffractometry (XRD) , scanning electron microsopy (SEM) and energy dis- persive spectroscopy (EDS). Moreover, the microhardness of the substrate and the coating are investigated using Vickers mierohardness tester, the friction and wear behaviors of the substrate and the coating are investigated by using a block-on-ring tribometer under dry sliding conditions with the load of 245 N. The results show that both γ-Al2O3 and α-Al2O3 phases are observed in the as-sprayed coatings, the mian phase is γ-Al2O3. There are white particulates Al2O3 on its surface. The Al2O3-13 wt. % TiO2 coating possesses higher mierohardness which is about 1018HV and 1.6 times that of the substrate. The wear performance of coating is better than that of the substrate. In a practical application, the life of the extrusion wheel which is plasma sprayed Al2O3-13 wt. % TiO2 coating on the surface is 1.2 times that of the conventional extrusion wheel, and the life is about 330 h.
基金National Natural Science Foundation of China (81473458)Chinese Medicine Science and Technology Program of Jiangsu Province (MS2021003)Jiangsu Province Phase V"333"Scientific Research Project (BRA2020164)。
基金financially sponsored by the National Natural Science Foundations of China(Nos.51590894,51425102,51231001)China Postdoctoral Science Foundation(No.2017T100023)。
文摘A novelγ-γ'(Ni solid solution—Ni_(3)Al)NiPtAl coating was produced on advanced single-crystal(SC)superalloy N5 by electroplating of a thin Pt coating with~3μm in thickness followed by heat treatment at 1000℃in vacuum.For comparison,a traditionalγ-γ'NiPtAl coating was also produced by electroplating of a thick Pt coating with~6μm in thickness followed by heat treatment at 1100℃in vacuum.The novel coating has a mass gain of~0.5 mg·cm^(-2)after 100 h 1-h cyclic oxidation at 1150℃,showing better oxidation resistance than the traditional coating.An oxide scale mostly consisting ofα-Al_(2)O_(3)grew on the novel coating because the element Cr from the SC alloy substrate contributes to the formation ofα-Al_(2)O_(3).Pt content in the novel coating has significant effect on the oxidation resistance.The optimized Pt content in the coating is about 16 at%,which is helpful to reduce the diffusion of harmful elements from the substrate into the coating but also to reduce the cost of the coating.
基金Project supported by the Science and Technology Commission of Shanghai Municipality,China(Grant Nos.13111102300 and 11dz1100302)the National Natural Science Foundation of China(Grant Nos.11174193 and 51202141)+1 种基金the National Basic Research Program of China(Grant Nos.2011CBA00105)the Science and Technology Commission of Shanghai Municipality,China(Grant No.14DZ2260700)
文摘It is significant for low-cost preparation of YBa2Cu3O7-δ(YBCO) coated conductors to make clear the mechanism of orientation, copper segregation, and nucleation density in BaF2-derived YBCO crystallization. In the present work,a distinct nucleation mechanism was proposed based on a transient liquid phase induced by the size effect as well as near-equilibrium assumption. With this scheme the nucleation of YBCO prepared by metal–organic deposition(MOD) or the physical vapor deposition BaF2 process was semi-quantitatively analyzed, revealing that the direct driving force for nucleation is YBCO supersaturation in the liquid phase. The theoretical analysis on the nucleation orientation portion is evidenced by the experimental result.