Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size ...Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.展开更多
Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were domin...Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index (REI) showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.展开更多
To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corr...To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corresponding age classes. A static life table was prepared. Moreover, the curves of survival rate, mortality rate and killing powder were drawn so as to perform spectral analysis for Toona clliata in Qizimeishan Nature Reserve. The results showed that the Vpi and Vpf of the Toona ci/iata population were 32.32% and 4.039%, respectively, indicating that Toona ci/iata is a stable growth-oriented population with high risk sensitivity. The survival rate curve was approximately fitted to the Deevey II type, so the individuals in each age class had an equivalent mortality rate in overall. The qx and Kx all reached peaks si- multaneously at 1, 4 and 6 age classes, indicating great effects of physiological properties, environmental sieves and human disturbances on Toona ci/iata population at various growth stages. The spectral analysis showed that the fundamental wave A1 was 0.752 8, while A3 showed an obvious minor cycle deviating from AI. It suggests that there is a periodicity, with minor cycles of multi-harmonic superposition, in natural regeneration of Toona ciliata population.展开更多
The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and...The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.展开更多
In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found onl...In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found only existing T. cili- ata Roem. communities (T1 and T2) with contiguous grid quadrate method, in the Nanhe River valley, Gucheng County. By X2 test, t-test of distribution coefficient Cx method, and F test of Morisita pattern index Iδ whether the distribution patterns of the T. ciliata Roem. populations conformed to Poisson distribution were checked. The results indicated that, population T1 was in Poisson distribution under 5 m×5 m and 3 m×3 m in size by Cx and 16 methods, but in clumped distribution pattern un- der 5 m×5 m in size by the Chi-square test; however, the population was in Pois- son distribution under the dimension of 3 m×3 m. Population T2 under human dis- turbance had higher population density, indicating clumped distribution under 3 above-mentioned tests. If Chi-square test is satisfied, a distribution pattern is in Poisson distribution, and size and quantity of sampling quadrats should be given pri- ority to; and if df is greater, both theoretical values and observed values tend to- wards normal distribution more probably, and the test of distribution pattern, there- fore will be more dependable.展开更多
We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effe...We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.展开更多
With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,...With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,we used the data collected from the field survey and employed different ecological niche models.The models tested were the broken stick model(BSM),the overlapping niche model(ONM)and the niche preemption model(NPM),as well as three statistic models,the log-series distribution model(LSD),the log-normal distribution model(LND)and the Weibull distribution model(WDM).To determine the fitted model most suitable to each layer,the fitting effects were judged by criteria of the lowest value of Akaike Information Criterion(AIC),Chi-square and the K-S values with no significant difference(P>0.05)between the theoretical predictions and observed species abundance distribution values.The result showed:(1)The fitting suitability and goodness of fit of the tree,shrub and herb layers by using the three ecological niche models were ranked as:NPM>BSM>ONM.Of the three statistical models,by accepting the fitting results of the three layers,WDM was the best fitting model,followed by LND.By rejecting the fitting tests of the herb layer,LSD had the worst fitting effect.The goodness of the statistical models was ranked as:WDM>LND>LSD.In general,the statistical models had better fitting results than the ecological models.(2)T.ciliata was the dominant species of the tree layer.The species richness and diversity of the herb layer were much higher than those of either the tree layer or the shrub layer.The species richness and diversity of the shrub layer were slightly higher than those of the tree layer.The community evenness accorded to the following order:herb>shrub>tree.Considering the fitting results of the different layers,different ecological niche models or statistical models with optimal goodness of fit and ecological significance can be given priority to in studying the species abundance distribution patterns of T.ciliata communities.展开更多
In order to provide a theoretical basis for the protection and development of T. ciliata germplasm resources, we studied the genetic diversity of T. ciliata by using SSR(Simple Sequence Repeat) primers to evaluate the...In order to provide a theoretical basis for the protection and development of T. ciliata germplasm resources, we studied the genetic diversity of T. ciliata by using SSR(Simple Sequence Repeat) primers to evaluate the genetic diversity of 192 T. ciliata germplasm samples from 24 populations of 5 provinces. Data Formater, Popgene, NTSYS, TFPGA and other software were used for genetic data conversion, genetic parameter estimation, dendrogram construction and genetic variation analysis. The results showed that: 1) a total of 17 alleles(Na) were detected in seven pairs of primers, with an average of 2.260 for each primer. Among them, the highest numbers of alleles(4) were detected in primers S11 and S422.The mean value of Nei’s genetic diversity index(H) was 0.4909, the mean value of Shannon information index(I) was 0.7321, and the mean value of polymorphic information content(PIC) was 0.5182. The mean expected heterozygosity(He) and observed heterozygosity(Ho) were 0.1055 and 0.4956, respectively. The Nei’s genetic distances of the populations ranged between 0.0002 and 2.6346, and the mean was 0.5477. The average genetic diversity level(H=0.1044) of the 24 populations was lower than that of the species(H=0.4909). 2) The genetic differentiation coefficients(Fst) varied from 0.2374 to 0.9148, with an average value of 0.7727. The mean of population gene flow(Nm) was 0.0735, indicating a low level of genetic exchange between populations, and suggesting that the genetic variation mainly came from within populations. 3) With the UPGMA method, the 24 populations were clustered into 3 groups at Nei’s genetic identity(0.99): the populations from Guizhou Province and Guangxi Zhuang Autonomous Region were clustered into one group, the populations from Hunan Province were in another group, and the populations from Hubei Province were in the third group. The Mantel test analysis showed a significant correlation between Nei’s genetic distance and geographic distance(r=0.6318, P=0.009<0.05). The genetic diversity of the 24 populations of T. ciliata was at a low level. Geographic isolation was the main reason for genetic differentiation among T. ciliata provenances. In the protection of germplasm resources of T. ciliata, emphasis should be placed on breeding genetic resources from the populations with higher genetic diversity(P14, for example). As for the populations with low genetic diversity, an ex-situ protection strategy as well as ecological and timber objectives, should be taken into account to maximize the conservation and utilization of the diversity of T. ciliata.展开更多
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
文摘Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.
基金Supported by Hunan Forestry Science and Technology Project(XLK201406)~~
文摘Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows: Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index (REI) showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.
基金Supported by Special Scientific Research Fund of Public Welfare Profession of Department of Science and Technology of Hubei Province(40 2012DBA40001)~~
文摘To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corresponding age classes. A static life table was prepared. Moreover, the curves of survival rate, mortality rate and killing powder were drawn so as to perform spectral analysis for Toona clliata in Qizimeishan Nature Reserve. The results showed that the Vpi and Vpf of the Toona ci/iata population were 32.32% and 4.039%, respectively, indicating that Toona ci/iata is a stable growth-oriented population with high risk sensitivity. The survival rate curve was approximately fitted to the Deevey II type, so the individuals in each age class had an equivalent mortality rate in overall. The qx and Kx all reached peaks si- multaneously at 1, 4 and 6 age classes, indicating great effects of physiological properties, environmental sieves and human disturbances on Toona ci/iata population at various growth stages. The spectral analysis showed that the fundamental wave A1 was 0.752 8, while A3 showed an obvious minor cycle deviating from AI. It suggests that there is a periodicity, with minor cycles of multi-harmonic superposition, in natural regeneration of Toona ciliata population.
基金Hubei Provincial Department of Science and Technology,under the public welfare research project[No.402012DBA40001]Hubei Provincial Department of Education,under the scientifi c research project[No.B20160555].
文摘The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.
基金Supported by Public Welfare Research Project of Science and Technology Agency of Hubei Province(402012DBA40001)~~
文摘In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found only existing T. cili- ata Roem. communities (T1 and T2) with contiguous grid quadrate method, in the Nanhe River valley, Gucheng County. By X2 test, t-test of distribution coefficient Cx method, and F test of Morisita pattern index Iδ whether the distribution patterns of the T. ciliata Roem. populations conformed to Poisson distribution were checked. The results indicated that, population T1 was in Poisson distribution under 5 m×5 m and 3 m×3 m in size by Cx and 16 methods, but in clumped distribution pattern un- der 5 m×5 m in size by the Chi-square test; however, the population was in Pois- son distribution under the dimension of 3 m×3 m. Population T2 under human dis- turbance had higher population density, indicating clumped distribution under 3 above-mentioned tests. If Chi-square test is satisfied, a distribution pattern is in Poisson distribution, and size and quantity of sampling quadrats should be given pri- ority to; and if df is greater, both theoretical values and observed values tend to- wards normal distribution more probably, and the test of distribution pattern, there- fore will be more dependable.
基金This work was fi nancially supported by the National Key Research Projects,Forestry Resource Cultivation and Utilization Technology Innovation(Grant No.2016YFD0600606)the Natural Science Foundation of Guangdong Province of China(Grant No.2018A030313798)Characteristic innovation projects of department of education of Guangdong province(Grant No.2019KTSCX017).
文摘We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.
基金Public Welfare Research Project of Department of Science and Technology in Hubei Province(40 2012DBA40001)Scientific Research Project of Department of Education in Hubei Province(B20160555)
文摘With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,we used the data collected from the field survey and employed different ecological niche models.The models tested were the broken stick model(BSM),the overlapping niche model(ONM)and the niche preemption model(NPM),as well as three statistic models,the log-series distribution model(LSD),the log-normal distribution model(LND)and the Weibull distribution model(WDM).To determine the fitted model most suitable to each layer,the fitting effects were judged by criteria of the lowest value of Akaike Information Criterion(AIC),Chi-square and the K-S values with no significant difference(P>0.05)between the theoretical predictions and observed species abundance distribution values.The result showed:(1)The fitting suitability and goodness of fit of the tree,shrub and herb layers by using the three ecological niche models were ranked as:NPM>BSM>ONM.Of the three statistical models,by accepting the fitting results of the three layers,WDM was the best fitting model,followed by LND.By rejecting the fitting tests of the herb layer,LSD had the worst fitting effect.The goodness of the statistical models was ranked as:WDM>LND>LSD.In general,the statistical models had better fitting results than the ecological models.(2)T.ciliata was the dominant species of the tree layer.The species richness and diversity of the herb layer were much higher than those of either the tree layer or the shrub layer.The species richness and diversity of the shrub layer were slightly higher than those of the tree layer.The community evenness accorded to the following order:herb>shrub>tree.Considering the fitting results of the different layers,different ecological niche models or statistical models with optimal goodness of fit and ecological significance can be given priority to in studying the species abundance distribution patterns of T.ciliata communities.
基金The Public Welfare Research Project of Department of Science and Technology in Hubei Province (402012DBA40001)The Scientific Research Project of Department of Education in Hubei Province (B20160555)。
文摘In order to provide a theoretical basis for the protection and development of T. ciliata germplasm resources, we studied the genetic diversity of T. ciliata by using SSR(Simple Sequence Repeat) primers to evaluate the genetic diversity of 192 T. ciliata germplasm samples from 24 populations of 5 provinces. Data Formater, Popgene, NTSYS, TFPGA and other software were used for genetic data conversion, genetic parameter estimation, dendrogram construction and genetic variation analysis. The results showed that: 1) a total of 17 alleles(Na) were detected in seven pairs of primers, with an average of 2.260 for each primer. Among them, the highest numbers of alleles(4) were detected in primers S11 and S422.The mean value of Nei’s genetic diversity index(H) was 0.4909, the mean value of Shannon information index(I) was 0.7321, and the mean value of polymorphic information content(PIC) was 0.5182. The mean expected heterozygosity(He) and observed heterozygosity(Ho) were 0.1055 and 0.4956, respectively. The Nei’s genetic distances of the populations ranged between 0.0002 and 2.6346, and the mean was 0.5477. The average genetic diversity level(H=0.1044) of the 24 populations was lower than that of the species(H=0.4909). 2) The genetic differentiation coefficients(Fst) varied from 0.2374 to 0.9148, with an average value of 0.7727. The mean of population gene flow(Nm) was 0.0735, indicating a low level of genetic exchange between populations, and suggesting that the genetic variation mainly came from within populations. 3) With the UPGMA method, the 24 populations were clustered into 3 groups at Nei’s genetic identity(0.99): the populations from Guizhou Province and Guangxi Zhuang Autonomous Region were clustered into one group, the populations from Hunan Province were in another group, and the populations from Hubei Province were in the third group. The Mantel test analysis showed a significant correlation between Nei’s genetic distance and geographic distance(r=0.6318, P=0.009<0.05). The genetic diversity of the 24 populations of T. ciliata was at a low level. Geographic isolation was the main reason for genetic differentiation among T. ciliata provenances. In the protection of germplasm resources of T. ciliata, emphasis should be placed on breeding genetic resources from the populations with higher genetic diversity(P14, for example). As for the populations with low genetic diversity, an ex-situ protection strategy as well as ecological and timber objectives, should be taken into account to maximize the conservation and utilization of the diversity of T. ciliata.