Bivalent histone modifications during tooth development

Histone methylation is one of the most widely studied post-transcriptional modifications. It is thought to be an important epigenetic event that is closely associated with cell fate determination and differentiation. To explore the spatiotemporal expression of histone H3 lysine 4trimethylation（H3K4me3） and histone H3 lysine 27 trimethylation（H3K27me3） epigenetic marks and methylation or demethylation transferases in tooth organ development, we measured the expression of SET7, EZH2, KDM5 B and JMJD3 via immunohistochemistry and quantitative polymerase chain reaction（qP CR） analysis in the first molar of BALB/c mice embryos at E13.5, E15.5, E17.5, P0 and P3, respectively. We also measured the expression of H3K4me3 and H3K27me3 with immunofluorescence staining. During murine tooth germ development, methylation or demethylation transferases were expressed in a spatial–temporal manner. The bivalent modification characterized by H3K4me3 and H3K27me3 can be found during the tooth germ development, as shown by immunofluorescence. The expression of SET7, EZH2 as methylation transferases and KDM5 B and JMJD3 as demethylation transferases indicated accordingly with the expression of H3K4me3 and H3K27me3 respectively to some extent. The bivalent histone may play a critical role in tooth organ development via the regulation of cell differentiation.

Statistical Modification Analysis of Helical Planetary Gears based on Response Surface Method and Monte Carlo Simulation

Tooth modification technique is widely used in gear industry to improve the meshing performance of gearings. However, few of the present studies on tooth modification considers the influence of inevitable random errors on gear modification effects. In order to investigate the uncertainties of tooth modification amount variations on system＇s dynamic behaviors of a helical planetary gears, an analytical dynamic model including tooth modification parameters is proposed to carry out a deterministic analysis on the dynamics of a helical planetary gear. The dynamic meshing forces as well as the dynamic transmission errors of the sun-planet 1 gear pair with and without tooth modifications are computed and compared to show the effectiveness of tooth modifications on gear dynamics enhancement. By using response surface method, a fitted regression model for the dynamic transmission error（DTE） fluctuations is established to quantify the relationship between modification amounts and DTE fluctuations. By shifting the inevitable random errors arousing from manufacturing and installing process to tooth modification amount variations, a statistical tooth modification model is developed and a methodology combining Monte Carlo simulation and response surface method is presented for uncertainty analysis of tooth modifications. The uncertainly analysis reveals that the system＇s dynamic behaviors do not obey the normal distribution rule even though the design variables are normally distributed. In addition, a deterministic modification amount will not definitely achieve an optimal result for both static and dynamic transmission error fluctuation reduction simultaneously.

Investigation of a Design Modification for Double Helical Gears Reducing Vibration and Noise

To reduce vibration and noise and increase transmission efficiency, a three segment method for modifying the pinion profile was proposed. Cutter surface equations were deduced by changing the shape of the cutter-edge, substituting three segment parabolas for the line. The influence of longitudinal tooth modifications on tooth surface load distributions was discussed. Transmission error minimization and uniformity of tooth surface load distribution were chosen as optimization goals and the modified parameters were obtained by applying the complex method. Finally, an experiment comparing the loaded transmission error, vibration, and noise both before and after modifications was carried out. The results indicate that the modified design is reliable.

Lubrication characteristics of cycloid pin wheel transmission of RV reducer

In order to analyze the lubricating characteristics at different meshing points in the cycloid pin wheel transmission process,the cycloid gear teeth were discretized,combined with the kinematic analysis of the cycloid pin gear transmission and the contact analysis of the gear teeth.The progressive mesh densification method(PMD)was used to numerically solve the film thickness.The influence of the design parameters and process parameters on the lubrication characteristics was analyzed.The elastohydrodynamic lubrication and mixed lubrication characteristics at different contact points were obtained.The optimal meshing area of the cycloid gear tooth was determined,and the film thickness ratio,contact load ratio,maximum contact pressure at different points,average film thickness and roughness after contact deformation were analyzed.The conclusion of this study provides effective guidance for the research on the modification of cycloid gear teeth.

MESHING THEORY AND DESIGN METHOD OF NEW SILENT CHAIN AND SPROCKET

Based on the study of the meshing theory of a new silent chain and sprockets, and the rolling cutting theory of sprocket and hob, the harmonious relations of dominating dimensions among the new silent chain, sprocket and hob is build, the meshing conditions are expatiated, and the resolved expression, which can instruct design and calculation, is educed. The tests show that the meshing design method is feasible.

DIRECT DIGITAL DESIGN AND SIMULATION OF MESHING IN WORM-GEAR DRIVE

A direct digital design method （DDDM） of worm-gear drive is proposed. It is directly based on the simulation of manufacturing process and completely different from the conventional modeling method. The loaded tooth contact analysis （LTCA） method is analyzed, in which the advanced surface to surface searching technique is included. The influence of misalignment errors and contact deformations on contact zone and transmission error （TE） is discussed. Combined modification approach on worm tooth surface is presented. By means of DDDM and LTCA, it is very conven- ient to verify the effect of worm-gear drive＇s modification approach. The analysis results show that, the modification in profile direction reduces the sensitivity of worm-gear drive to misalignment errors and the modification in longitudinal direction decreases the TE. Thus the optimization design of worm-gear drive can be achieved prior to the actual manufacturing process.