Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The nut has a variety of bioactive compounds, such as polyphenols and vitamins. However, the carotenoids compositions an...Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The nut has a variety of bioactive compounds, such as polyphenols and vitamins. However, the carotenoids compositions and its regulatory mechanisms underlying carotenoid biosynthesis in kernels remain unknown. Here, the major carotenoids in the kernel were analyzed. The result showed that lutein and β-carotene were the predominant carotenoids in the kernel, while low levels of a-carotenoid and zeaxanthin were detected. Lutein and β-carotene were decreased during the maturation of kernel. Expression analysis by RNA-seq and q RT-PCR indicated that the expressions of TgCYP97A3 and TgLCYB were also reduced during the maturation of kernel. The contents of lutein or β-carotene were obviously increased in tobacco transiently overexpressing TgCYP97A3 or TgLCYB. Moreover, DPPH radical scavenging activity and FRAP were also significantly enhanced. In addition, several MYB and WRKY transcription factors TgMYBS3, TgMYB48 and TgWRKY11 were identified to positively regulate the TgCYP97A3 expression, while TgMYB48, TgWRKY2 and TgWRKY11 could upregulate the TgLCYB expression. The illustration of carotenoids biosynthesis and its molecular mechanism in kernels not only provides a basis for understanding carotenoids biosynthesis in kernels, but also enables the use of molecular biotechnology to develop new health products rich in carotenoids based on T. grandis nuts.展开更多
[Objectives]This study was conducted to prevent the occurrence of root rot disease in Torreya grandis and improve the yield and quality of T.grandis.[Methods]One-year-old and two-year-old seedlings of Torreya grandis...[Objectives]This study was conducted to prevent the occurrence of root rot disease in Torreya grandis and improve the yield and quality of T.grandis.[Methods]One-year-old and two-year-old seedlings of Torreya grandis‘Xifei’and‘Cufei’were inoculated with the root rot pathogen Fusarium fujikuroi,and the changes in photosynthesis,chlorophyll content,malondialdehyde,and defense enzyme system(superoxide dismutase,peroxidase,and catalase)activity in leaves of T.grandis seedlings were investigated using water as a control.Meanwhile,the control effects of 80%carbendazim wettable powder,64%metalaxyl·mancozeb wettable powder,430 g/L tebuconazole suspension and 30%difenoconazole·cyproconazole EC on root rot in T.grandis were investigated.[Results]After inoculation with the pathogen F.fujikuroi,the net photosynthetic rates and transpiration rates in leaves of T.grandis‘Xifei’and‘Cufei’decreased,and the contents of chlorophyll decreased,while the contents of malondialdehyde increased,and the contents of superoxide dismutase and catalase increased with time.However,peroxidase showed a high activity in T.grandis‘Cufei’only,but a trend of"increasing-decreasing-increasing"in T.grandis‘Xifei’.Among the four chemical control agents,64%metalaxyl·mancozeb wettable powder had the best control effect on root rot of T.grandis caused by the pathogenic fungus F.fujikuroi.[Conclusions]The net photosynthetic rate,transpiration rate,chlorophyll content,malondialdehyde content,superoxide dismutase activity and catalase activity could all be used as screening indicators for T.grandis varieties resistant to root rot.Meanwhile,64%metalaxyl·mancozeb wettable powder could be used as a control agent for root rot on T.grandis.展开更多
Torreya grandis cv.Merrillii(Taxace,Torreya)is mainly distributed across the hilly areas of subtropical China and is well known for its nutritional value.In this study,the ameliorative effects of T.grandis seed oil on...Torreya grandis cv.Merrillii(Taxace,Torreya)is mainly distributed across the hilly areas of subtropical China and is well known for its nutritional value.In this study,the ameliorative effects of T.grandis seed oil on lipid metabolism were investigated,and the underlying mechanism was explored from the perspective of gut microbiota.Mice experiments showed that the rate of body mass gain in the group where the mice were fed a high-fat diet(HFD)and supplemented with 550 mg/(kg·day)T.grandis seed oil(HFD+TO550 group)was 42.27%,while it was 62.25%in the HFD group.Compared with the HFD group,the liver and fat indices,total cholesterol,triglycerides,and low-density lipoprotein cholesterol were reduced in the oil-supplement groups.Moreover,the oil supplement significantly changed the fatty acid composition and alleviated pathological damage to the liver caused by the high-fat diet.Additionally,the distinct clustering of bacteria in the composition of gut microbiota was observed in the oil treatment group compared with that in the HFD group.T.grandis seed oil significantly increased the abundance of the beneficial bacteria and short-chain fatty acid producers,including Lactobacillus,Bifidobacterium,Faecalibaculum and Allobaculum.Our results suggest that the supplements of T.grandis seed oil could alleviate hyperlipidemia caused by HFD.These positive effects are considered to be related with sciadonic acid(SCA)and are partially mediated by alterations in gut microbiota composition and functionality.展开更多
[Objectives]This study aimed to investigate the differences in nutritional properties of nuts from different Torreya grandis cultivars.[Methods]Seven T.grandis cultivars were selected to analyze the protein,soluble ca...[Objectives]This study aimed to investigate the differences in nutritional properties of nuts from different Torreya grandis cultivars.[Methods]Seven T.grandis cultivars were selected to analyze the protein,soluble carbohydrate,vitamin E,amino acids and fatty acid composition,and evaluate nutritional value of T.grandis nuts from different cultivars using gray correlation analysis.[Results]The kernels of T.grandis contained 9.47%-13.3% protein,1.92%-2.36% soluble carbohydrate,0.16-0.22 mg/g vitamin E,8.40%-11.55% amino acids,and 42.09%-49.29% fatty acid including 35.14%-40.14% oleic acid.And the coefficient of variation ranged from 5.02% to 18.9% among different cultivars.Meanwhile,the average contents of essential amino acids(E),nonessential amino acids(N)and children essential amino acids(C)were 3.88%,6.07% and 0.99%,respectively.The ratios of E/T and E/N were quite stable and in fully compliance with the recommendation standards of amino acids composition from WHO/FAO.[Conclusions]Overall,the nutritional value of tested seven cultivars could be ranked in the following decreasing order using gray correlation analysis:T.grandis cv.Dong No.2>T.grandis cv.Longfeng>T.grandis cv.Dingshan>T.grandis cv.Merrilli>T.grandis cv.Dong No.3>T.grandis cv.Dong No.1>T.grandis cv.Zhuyan.展开更多
基金funded by the National Natural Science Foundation of China (Grant No.U20A2049)the Key Research and Development Program of Zhejiang Province (Grant No.2021C02001)the Scienti?c Research Startup Fund Project of Zhejiang A&F University (Grant No.2022LFR033)。
文摘Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The nut has a variety of bioactive compounds, such as polyphenols and vitamins. However, the carotenoids compositions and its regulatory mechanisms underlying carotenoid biosynthesis in kernels remain unknown. Here, the major carotenoids in the kernel were analyzed. The result showed that lutein and β-carotene were the predominant carotenoids in the kernel, while low levels of a-carotenoid and zeaxanthin were detected. Lutein and β-carotene were decreased during the maturation of kernel. Expression analysis by RNA-seq and q RT-PCR indicated that the expressions of TgCYP97A3 and TgLCYB were also reduced during the maturation of kernel. The contents of lutein or β-carotene were obviously increased in tobacco transiently overexpressing TgCYP97A3 or TgLCYB. Moreover, DPPH radical scavenging activity and FRAP were also significantly enhanced. In addition, several MYB and WRKY transcription factors TgMYBS3, TgMYB48 and TgWRKY11 were identified to positively regulate the TgCYP97A3 expression, while TgMYB48, TgWRKY2 and TgWRKY11 could upregulate the TgLCYB expression. The illustration of carotenoids biosynthesis and its molecular mechanism in kernels not only provides a basis for understanding carotenoids biosynthesis in kernels, but also enables the use of molecular biotechnology to develop new health products rich in carotenoids based on T. grandis nuts.
基金Supported by Youth Project of Natural Science Foundation of Anhui Province(2008085QC134)Natural Science Research Project in Colleges and Universities in Anhui Province(KJHS2019B09)+2 种基金School-level Talent Start-up Project(2020xkjq009)Key Project of Anhui Provincial Department of Education(2023AH051375)Key Project of Natural Science Research of Anhui Provincial Department of Education(KJ2020A0691).
文摘[Objectives]This study was conducted to prevent the occurrence of root rot disease in Torreya grandis and improve the yield and quality of T.grandis.[Methods]One-year-old and two-year-old seedlings of Torreya grandis‘Xifei’and‘Cufei’were inoculated with the root rot pathogen Fusarium fujikuroi,and the changes in photosynthesis,chlorophyll content,malondialdehyde,and defense enzyme system(superoxide dismutase,peroxidase,and catalase)activity in leaves of T.grandis seedlings were investigated using water as a control.Meanwhile,the control effects of 80%carbendazim wettable powder,64%metalaxyl·mancozeb wettable powder,430 g/L tebuconazole suspension and 30%difenoconazole·cyproconazole EC on root rot in T.grandis were investigated.[Results]After inoculation with the pathogen F.fujikuroi,the net photosynthetic rates and transpiration rates in leaves of T.grandis‘Xifei’and‘Cufei’decreased,and the contents of chlorophyll decreased,while the contents of malondialdehyde increased,and the contents of superoxide dismutase and catalase increased with time.However,peroxidase showed a high activity in T.grandis‘Cufei’only,but a trend of"increasing-decreasing-increasing"in T.grandis‘Xifei’.Among the four chemical control agents,64%metalaxyl·mancozeb wettable powder had the best control effect on root rot of T.grandis caused by the pathogenic fungus F.fujikuroi.[Conclusions]The net photosynthetic rate,transpiration rate,chlorophyll content,malondialdehyde content,superoxide dismutase activity and catalase activity could all be used as screening indicators for T.grandis varieties resistant to root rot.Meanwhile,64%metalaxyl·mancozeb wettable powder could be used as a control agent for root rot on T.grandis.
基金supported by the Regional Demonstration Project of Marine Economic Innovation and Development(2013 and 2016)the K.C.Wong Magna Fund offered by the Ningbo University.
文摘Torreya grandis cv.Merrillii(Taxace,Torreya)is mainly distributed across the hilly areas of subtropical China and is well known for its nutritional value.In this study,the ameliorative effects of T.grandis seed oil on lipid metabolism were investigated,and the underlying mechanism was explored from the perspective of gut microbiota.Mice experiments showed that the rate of body mass gain in the group where the mice were fed a high-fat diet(HFD)and supplemented with 550 mg/(kg·day)T.grandis seed oil(HFD+TO550 group)was 42.27%,while it was 62.25%in the HFD group.Compared with the HFD group,the liver and fat indices,total cholesterol,triglycerides,and low-density lipoprotein cholesterol were reduced in the oil-supplement groups.Moreover,the oil supplement significantly changed the fatty acid composition and alleviated pathological damage to the liver caused by the high-fat diet.Additionally,the distinct clustering of bacteria in the composition of gut microbiota was observed in the oil treatment group compared with that in the HFD group.T.grandis seed oil significantly increased the abundance of the beneficial bacteria and short-chain fatty acid producers,including Lactobacillus,Bifidobacterium,Faecalibaculum and Allobaculum.Our results suggest that the supplements of T.grandis seed oil could alleviate hyperlipidemia caused by HFD.These positive effects are considered to be related with sciadonic acid(SCA)and are partially mediated by alterations in gut microbiota composition and functionality.
基金Supported by Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(2016C02052-12).
文摘[Objectives]This study aimed to investigate the differences in nutritional properties of nuts from different Torreya grandis cultivars.[Methods]Seven T.grandis cultivars were selected to analyze the protein,soluble carbohydrate,vitamin E,amino acids and fatty acid composition,and evaluate nutritional value of T.grandis nuts from different cultivars using gray correlation analysis.[Results]The kernels of T.grandis contained 9.47%-13.3% protein,1.92%-2.36% soluble carbohydrate,0.16-0.22 mg/g vitamin E,8.40%-11.55% amino acids,and 42.09%-49.29% fatty acid including 35.14%-40.14% oleic acid.And the coefficient of variation ranged from 5.02% to 18.9% among different cultivars.Meanwhile,the average contents of essential amino acids(E),nonessential amino acids(N)and children essential amino acids(C)were 3.88%,6.07% and 0.99%,respectively.The ratios of E/T and E/N were quite stable and in fully compliance with the recommendation standards of amino acids composition from WHO/FAO.[Conclusions]Overall,the nutritional value of tested seven cultivars could be ranked in the following decreasing order using gray correlation analysis:T.grandis cv.Dong No.2>T.grandis cv.Longfeng>T.grandis cv.Dingshan>T.grandis cv.Merrilli>T.grandis cv.Dong No.3>T.grandis cv.Dong No.1>T.grandis cv.Zhuyan.
