To investigate the level of serum IL-10 in allergic rhinitis patients and the correlation between IL-10 and serum total IgE （TIgE）. 50 allergic rhinitis patients and 30 normal subjects were involved in the study. Th...To investigate the level of serum IL-10 in allergic rhinitis patients and the correlation between IL-10 and serum total IgE （TIgE）. 50 allergic rhinitis patients and 30 normal subjects were involved in the study. The levels of serum IL-10 and TIgE were measured by enzyme-linked immunosorbent assay （ELISA）, and the correlation between serum IL-10 and TIgE was analyzed. In the allergic rhinitis group, the levels of serum IL-10 and TIgE were 8.34±2.48 pg/mL and 142.6± 28.2 KUA/L. In the normal control group, they were 12.86±.88 pg/mL and 47.2±12.2 KUA. There were significant differences between the two groups （P〈0. 01）; and the level of serum TIgE in the patients was negatively correlated with that of IL-10 （r= -0.46, P=0. 02）. The level of serum IL-10 was significantly decreased in allergic rhinitis patients, which was beneficial to the synthesization of IgE. IL-10 plays an important role in the episode of allergic rhinitis.展开更多
BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding ...BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding region, so its polymorphism may result in the abnormality of APOE gene and protein expression, and finally lead to dementia. OBJECTIVE: To observe the association between APOE promoter-219G/T polymorphisms with serum total cholesterol in patients with Alzheimer disease, and compare it with non-dementia people. DESIGN: Case-control, comparative observation. SETTING: Department of Neurology, Fengtian Hospital of Shenyang Medical College. PARTICIPANTS: Fifty-five dementia patients including 27 males and 28 females aged (66±3) years and treated in the Department of Neurology, Fengtian Hospital were selected from January 2002 to December 2005 as the Alzheimer disease group. They all diagnosed according to the DSM-Ⅳdiagnostic criteria of Alzheimer disease instituted by American Psychiatry Association in 1994. Meanwhile, 44 none-dementia patients including 21 males and 23 females aged (66±3) years were selected from other clinical departments of Fengtian Hospital as control group. All the participants were informed the detection and agreed. METHODS: Genomic DNA was extracted from the peripheral blood of all subjects, then 'NEST'PCR, DNA sequence and enzyme digestion were adopted to detect the expression of APOE promoter-219 polymorphism, following by biomedical statistics analysis based on the clinical total cholesterol level. MAIN OUTCOME MEASURES: Polymorphism of APOE promoter-219 G/T and total cholesterol level. RESULTS: All 55 dementia patients and 44 non-dementia ones were involved in the result analysis. ①Allele and genotype frequency: The T allele frequency of the Alzheimer disease group was significantly higher than that in the control group [88.2% (97/110), 54.5% (48/88)], while G allele frequency was remarkably lower than that in the control group [11.8%(13/110), 45.5%(40/88), χ2=8.2, P < 0.01]. The TT allele frequency of the Alzheimer disease group was significantly higher than that in the control group [76% (42/55), 48% (21/44)], while GT+GG allele frequency was remarkably lower than that in the control group [24%(13/55), 52%(23/44), χ2=8.7, P < 0.01]. ②Total cholesterol level: The level of the TT genotype patients in the Alzheimer group was obviously higher than that in GT+GG genotype patients (t =2.46, P < 0.05); the cholesterol level in the two genotypes of the control group was similar (P > 0.05). CONCLUSION: TT genotype and allele T in the APOE promoter-219 polymorphisms are the sensitive gene, and genotype TT has a relationship with the increase of total cholesterol level.展开更多
The correlation between IgY levels of the serum and the yolk has been well documented in wild and domestic birds. The levels of total yolk IgY can be an index of the general health status of birds and may contribute t...The correlation between IgY levels of the serum and the yolk has been well documented in wild and domestic birds. The levels of total yolk IgY can be an index of the general health status of birds and may contribute to breeding programs when fitness of the offspring is a concern. We measured the levels of total serum IgY and yolk IgY in three different breeds （White Leghorn, Silkie and Dongxiang blue-shell） using indirect ELISA, and found that there was a significantly positive correlation between the levels of total serum IgY and total yolk IgY in all three breeds （White Leghorn： r = 0.404, P 〈 0.001, n = 100; Silkie： r = 0.561, P 〈 0.001, n = 70; Dongxiang blue-shell： r = 0.619, P 〈 0.001, n = 30）. We also measured the total serum IgY levels in the 3-day-old offspring hatched from the Silkie hens and results were significantly correlated for serum IgY levels （r = 0.535, P 〈 0.001, n = 70） and the yolk IgY levels （r = 0.481, P 〈 0.001, n = 70）. The regression analysis showed simple linear regression between IgY levels in hen serum, yolk and offspring serum. Our results suggest that total IgY level could be used as an index for chicken fitness.展开更多
Objective To investigate the effects of simvastatin compared with standard treatment on cardiovascular outcomes and all-cause mortality in high-risk hypertensive patients with a high–normal level of total cholesterol...Objective To investigate the effects of simvastatin compared with standard treatment on cardiovascular outcomes and all-cause mortality in high-risk hypertensive patients with a high–normal level of total cholesterol(TC).Methods In a randomized,open-label,blinded-endpoint trial,hypertensive patients at high risk for cardiovascular events from 180 clinical centers in China were assigned to receive treatment with amlodipine plus amiloride/hydrochlorothiazide(n=6,776)or telmisartan(n=6,766).展开更多
Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, ...Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, sex, ethnicity, and geographic area. This study set out to quantitatively determine the serum tIgE levels in Bangladeshi population who were clinically suspected cases of allergic diseases and to find out the association of tIgE level with their age and gender. This cross-sectional descriptive exploratory study was conducted at Biomed Diagnostics Laboratory, Panthapath, Dhaka, from March 2021-September 2021. A total of 402 blood samples were collected from clinically suspected cases of allergic diseases. Quantitative detection of tIgE in serum was done by Immunoblot assay manufactured by Proteome Tech Inc. (PROTIATM Allergy-Q64S panel). Among 402 subjects, a wide variation of tIgE levels ranging from 5 to 2000 IU/ml was found but majority of the study population (30.35%) showed tIgE levels within the range of 201 - 350 IU/ml. The study population aging 1 - 10 years showed highest frequency (23.63%) in tIgE concentrations and the mean tIgE level exhibited a declining tendency with an increase in age. Depending on gender there was no difference in tIgE levels. Determination of tIgE level is imperative for screening allergic diseases. The present study enlightened the distribution of tIgE levels and suggested that among Bangladeshi subjects irrespective of gender there is an inverse relationship between tIgE level and age.展开更多
The relationship between murine IgE, IgG, and IgG1 level and birosarcoma induced by 3-Methyl-cholanthrene in NIH mice was studied. The results showed that IgE, IgG or IgGl had no significant effect on tumor incidence;...The relationship between murine IgE, IgG, and IgG1 level and birosarcoma induced by 3-Methyl-cholanthrene in NIH mice was studied. The results showed that IgE, IgG or IgGl had no significant effect on tumor incidence; the high IgE level could extend the survival time of 3-Methylcholanthrene injected mice (P<0.05) and survival time of tumor bearing mice (P<0.01), while IgG or IgGl could not. The data from this study did not show that IgE, IgG or IgGl had any significant effect on the tumor size and the tumor growth rate. The probable mechanism involved was discussed.展开更多
Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from ...Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from the infant ward of our hospital from February 2012 to December 2012.A total of 120 patients were selected and recruited.Among these patients,64 patients with symptoms of allergic diseases were assigned to the experimental group.The remaining 56 patients who had respiratory infections,but without other symptoms of allergic diseases,were assigned to the control group.The serum 25-hydroxyvitamin D3 levels were measured using liquid chromatography tandem mass spectrometry and the IgE levels were measured using radioimmunoassay in both experimental and control groups.Subsequently,the serum 25-hydroxyvitamin D3 levels and IgE levels were compared between the experimental group and control group.Results:The following findings were obtained through data comparison.The serum 25-hydroxyvitamin D3 level in the experimental group was 18.19±7.84 ng/mL,and the IgE level was 551.51±705.88 IU/mL.The serum 25-hydroxyvitamin D3 level in the control group was 21.95±7.834 ng/mL,IgE level was 49.39±32.46 IU/mL.The data comparison indicates that the serum 25-hydroxyvitamin D3 level in the control group was higher than the experimental group,and the IgE level in the control group was lower than the experimental group(P<0.05).Conclusion:The level of vitamin D3 in patients with allergic symptoms is relatively low.This may be one of the causes leading to allergies.At the same time,IgE is also one of the causes contributing to allergies.An elevation of the IgE level is very likely to cause the allergic,and there is a correlation between serum vitamin D and IgE levels.At the same time,research has found that proper supplementation of vitamin D3 can help prevent allergic diseases,and can be considered in a new and highquality program for the prevention of allergic diseases.展开更多
文摘To investigate the level of serum IL-10 in allergic rhinitis patients and the correlation between IL-10 and serum total IgE （TIgE）. 50 allergic rhinitis patients and 30 normal subjects were involved in the study. The levels of serum IL-10 and TIgE were measured by enzyme-linked immunosorbent assay （ELISA）, and the correlation between serum IL-10 and TIgE was analyzed. In the allergic rhinitis group, the levels of serum IL-10 and TIgE were 8.34±2.48 pg/mL and 142.6± 28.2 KUA/L. In the normal control group, they were 12.86±.88 pg/mL and 47.2±12.2 KUA. There were significant differences between the two groups （P〈0. 01）; and the level of serum TIgE in the patients was negatively correlated with that of IL-10 （r= -0.46, P=0. 02）. The level of serum IL-10 was significantly decreased in allergic rhinitis patients, which was beneficial to the synthesization of IgE. IL-10 plays an important role in the episode of allergic rhinitis.
文摘探讨俞募配穴针法联合穴位埋针法治疗风痰闭阻型支气管哮喘的疗效及对血清免疫球蛋白E(Immunoglobin E,IgE)、可溶性二聚体细胞因子(Interferon-γ,IFN-γ)、肺表面活性蛋白(Pulmonary surfactant protein,SP-A)水平影响。方法选取医院收治的风痰闭阻型支气管哮喘患者108例,采用区组随机化原则分为两组各54例。对照组给予穴位埋针法,治疗组在对照组基础上给予俞募配穴针法,两组患者数据观察:对比治疗后临床疗效、哮喘控制测试(Asthma control test,ACT)量表评分、第一秒最大呼气容积(Forced expiratory volume in one second,FEV1)、呼气峰值流速(Peak expiratory,PEF)、用力肺活量(Forced vital capacity,FVC)、临床症状评分及IgE、IFN-γ、SP-A水平、临床治疗安全性及患者满意度。结果经过治疗后,治疗组临床疗效显著较高(P<0.05);两组治疗前ACT评分差异无统计学意义(P>0.05);治疗后两组ACT评分明显升高(P<0.05);且治疗组升高较明显(P<0.05);治疗后两组FEV1、PEF、FVC值明显升高(P<0.05);且治疗组升高较明显(P<0.05);两组治疗前临床症状评分差异无统计学意义(P>0.05);两组治疗后咳嗽、呼吸困难、喘息等症状评分显著降低(P<0.05);且治疗组降低较明显(P<0.05);两组治疗前血清IgE、IFN-γ、SP-A水平无差异(P>0.05);两组治疗后血清IgE显著降低,血清IFN-γ、SP-A水平显著升高(P<0.05);且治疗组改善较明显(P<0.05);治疗组未见治疗不良反应,对照组患者出现1例不良反应,症状为恶心,P>0.05;治疗组治疗满意率高达98.15%(53/54),显著高于对照组的83.33%(45/54),P<0.05。结论采用俞募配穴针法联合穴位埋针法治疗风痰闭阻型支气管哮喘具有较好的治疗效果,能够改善血清IgE、IFN-γ及SP-A水平,且本次治疗安全可靠,患者十分认可,值得在临床上推广应用。
文摘BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding region, so its polymorphism may result in the abnormality of APOE gene and protein expression, and finally lead to dementia. OBJECTIVE: To observe the association between APOE promoter-219G/T polymorphisms with serum total cholesterol in patients with Alzheimer disease, and compare it with non-dementia people. DESIGN: Case-control, comparative observation. SETTING: Department of Neurology, Fengtian Hospital of Shenyang Medical College. PARTICIPANTS: Fifty-five dementia patients including 27 males and 28 females aged (66±3) years and treated in the Department of Neurology, Fengtian Hospital were selected from January 2002 to December 2005 as the Alzheimer disease group. They all diagnosed according to the DSM-Ⅳdiagnostic criteria of Alzheimer disease instituted by American Psychiatry Association in 1994. Meanwhile, 44 none-dementia patients including 21 males and 23 females aged (66±3) years were selected from other clinical departments of Fengtian Hospital as control group. All the participants were informed the detection and agreed. METHODS: Genomic DNA was extracted from the peripheral blood of all subjects, then 'NEST'PCR, DNA sequence and enzyme digestion were adopted to detect the expression of APOE promoter-219 polymorphism, following by biomedical statistics analysis based on the clinical total cholesterol level. MAIN OUTCOME MEASURES: Polymorphism of APOE promoter-219 G/T and total cholesterol level. RESULTS: All 55 dementia patients and 44 non-dementia ones were involved in the result analysis. ①Allele and genotype frequency: The T allele frequency of the Alzheimer disease group was significantly higher than that in the control group [88.2% (97/110), 54.5% (48/88)], while G allele frequency was remarkably lower than that in the control group [11.8%(13/110), 45.5%(40/88), χ2=8.2, P < 0.01]. The TT allele frequency of the Alzheimer disease group was significantly higher than that in the control group [76% (42/55), 48% (21/44)], while GT+GG allele frequency was remarkably lower than that in the control group [24%(13/55), 52%(23/44), χ2=8.7, P < 0.01]. ②Total cholesterol level: The level of the TT genotype patients in the Alzheimer group was obviously higher than that in GT+GG genotype patients (t =2.46, P < 0.05); the cholesterol level in the two genotypes of the control group was similar (P > 0.05). CONCLUSION: TT genotype and allele T in the APOE promoter-219 polymorphisms are the sensitive gene, and genotype TT has a relationship with the increase of total cholesterol level.
基金supported in part by special funds from the China Agriculture Research System(CARS-41)the Yangtze River Scholar and Innovation Research Team Development Program(IRT0945)the Beijing Poultry Research System
文摘The correlation between IgY levels of the serum and the yolk has been well documented in wild and domestic birds. The levels of total yolk IgY can be an index of the general health status of birds and may contribute to breeding programs when fitness of the offspring is a concern. We measured the levels of total serum IgY and yolk IgY in three different breeds （White Leghorn, Silkie and Dongxiang blue-shell） using indirect ELISA, and found that there was a significantly positive correlation between the levels of total serum IgY and total yolk IgY in all three breeds （White Leghorn： r = 0.404, P 〈 0.001, n = 100; Silkie： r = 0.561, P 〈 0.001, n = 70; Dongxiang blue-shell： r = 0.619, P 〈 0.001, n = 30）. We also measured the total serum IgY levels in the 3-day-old offspring hatched from the Silkie hens and results were significantly correlated for serum IgY levels （r = 0.535, P 〈 0.001, n = 70） and the yolk IgY levels （r = 0.481, P 〈 0.001, n = 70）. The regression analysis showed simple linear regression between IgY levels in hen serum, yolk and offspring serum. Our results suggest that total IgY level could be used as an index for chicken fitness.
文摘Objective To investigate the effects of simvastatin compared with standard treatment on cardiovascular outcomes and all-cause mortality in high-risk hypertensive patients with a high–normal level of total cholesterol(TC).Methods In a randomized,open-label,blinded-endpoint trial,hypertensive patients at high risk for cardiovascular events from 180 clinical centers in China were assigned to receive treatment with amlodipine plus amiloride/hydrochlorothiazide(n=6,776)or telmisartan(n=6,766).
文摘Allergy is an adverse immunologic response of increasing prevalence and severity in which immunoglobulin E plays a pivotal role. Total IgE (tIgE) levels show wide variations depending on various factors, such as age, sex, ethnicity, and geographic area. This study set out to quantitatively determine the serum tIgE levels in Bangladeshi population who were clinically suspected cases of allergic diseases and to find out the association of tIgE level with their age and gender. This cross-sectional descriptive exploratory study was conducted at Biomed Diagnostics Laboratory, Panthapath, Dhaka, from March 2021-September 2021. A total of 402 blood samples were collected from clinically suspected cases of allergic diseases. Quantitative detection of tIgE in serum was done by Immunoblot assay manufactured by Proteome Tech Inc. (PROTIATM Allergy-Q64S panel). Among 402 subjects, a wide variation of tIgE levels ranging from 5 to 2000 IU/ml was found but majority of the study population (30.35%) showed tIgE levels within the range of 201 - 350 IU/ml. The study population aging 1 - 10 years showed highest frequency (23.63%) in tIgE concentrations and the mean tIgE level exhibited a declining tendency with an increase in age. Depending on gender there was no difference in tIgE levels. Determination of tIgE level is imperative for screening allergic diseases. The present study enlightened the distribution of tIgE levels and suggested that among Bangladeshi subjects irrespective of gender there is an inverse relationship between tIgE level and age.
文摘The relationship between murine IgE, IgG, and IgG1 level and birosarcoma induced by 3-Methyl-cholanthrene in NIH mice was studied. The results showed that IgE, IgG or IgGl had no significant effect on tumor incidence; the high IgE level could extend the survival time of 3-Methylcholanthrene injected mice (P<0.05) and survival time of tumor bearing mice (P<0.01), while IgG or IgGl could not. The data from this study did not show that IgE, IgG or IgGl had any significant effect on the tumor size and the tumor growth rate. The probable mechanism involved was discussed.
文摘Objective:To investigate the levels of serum vitamin D and IgE in infants with different allergic risks and to analyze their correlations.Methods:In the present study,the participants were selected and recruited from the infant ward of our hospital from February 2012 to December 2012.A total of 120 patients were selected and recruited.Among these patients,64 patients with symptoms of allergic diseases were assigned to the experimental group.The remaining 56 patients who had respiratory infections,but without other symptoms of allergic diseases,were assigned to the control group.The serum 25-hydroxyvitamin D3 levels were measured using liquid chromatography tandem mass spectrometry and the IgE levels were measured using radioimmunoassay in both experimental and control groups.Subsequently,the serum 25-hydroxyvitamin D3 levels and IgE levels were compared between the experimental group and control group.Results:The following findings were obtained through data comparison.The serum 25-hydroxyvitamin D3 level in the experimental group was 18.19±7.84 ng/mL,and the IgE level was 551.51±705.88 IU/mL.The serum 25-hydroxyvitamin D3 level in the control group was 21.95±7.834 ng/mL,IgE level was 49.39±32.46 IU/mL.The data comparison indicates that the serum 25-hydroxyvitamin D3 level in the control group was higher than the experimental group,and the IgE level in the control group was lower than the experimental group(P<0.05).Conclusion:The level of vitamin D3 in patients with allergic symptoms is relatively low.This may be one of the causes leading to allergies.At the same time,IgE is also one of the causes contributing to allergies.An elevation of the IgE level is very likely to cause the allergic,and there is a correlation between serum vitamin D and IgE levels.At the same time,research has found that proper supplementation of vitamin D3 can help prevent allergic diseases,and can be considered in a new and highquality program for the prevention of allergic diseases.
