Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Al...Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.展开更多
Objective:The moisture content in the soil directly affects the yield and quality of Panax notoginseng,especially at the age of three years old.However,the suitable moisture for the growth of P.notoginseng is unknown....Objective:The moisture content in the soil directly affects the yield and quality of Panax notoginseng,especially at the age of three years old.However,the suitable moisture for the growth of P.notoginseng is unknown.In this study,the effects of different soil moisture on the growth of P.notoginseng were studied.Methods:Four different water treatments(0.45 field capacity(FC),0.60 FC, 0.70 FC, and 0.85 FC) were set up in Shilin County,Yunnan Province,China.The water consumption and daily dynamic of water consumption were determined daily(from April 21 to October 18,2012),and the daily dynamic of water consumption under different weather conditions(sunny and rainy) was determined.The transpiration coefficient and water use efficiency were calculated through dry matter accumulation and total water consumption.Accumulation of saponins of roots of P.notoginseng were analyzed by HPLC after treated,and the soil moisture content suitable for the growth of P.notoginseng was estimated by regression fitting of the active ingredient accumulation and the soil moisture content.Results:The water consumption of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.89,3.68,3.37 and 2.73 kg/plant per day,respectively.The water consumption of P.notoginseng from June to August was greater than other months.The daily dynamic of water consumption on sunny days and sunny days after rain showed a "double peak" feature,and it showed a "single peak" feature on rainy days.The water uses efficiency(WUE) of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.51,3.32,4.59,3.39 gDW/kg H_(2)O,respectively.The increase of soil moistu re content would reduce the WUE of P.notoginse ng.With the increase of soil water content,the content of notoginsenoside R_(1) and ginsenoside Rg_(1) did not change significantly,while the content of ginsenoside Rb_(1) and Rd showed a decreasing trend.Conclusion:Soil moisture content significantly affected the water consumption of P.notoginseng,and when it was 56.4% of the maximum water holding capacity in the field,the sum of the four saponins of 100 strains of P.notoginseng was the highest.展开更多
基金ZHAO Yu-qing was supportive with a Liaoning Modernization TCM grant (LN403004),China
文摘Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.
基金supported by National Natural Science Foundation of China (81703641)Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (2060302)+1 种基金China Postdoctoral Science Foundation (2020T130601)National key Research and development program (2017YFC1700704)。
文摘Objective:The moisture content in the soil directly affects the yield and quality of Panax notoginseng,especially at the age of three years old.However,the suitable moisture for the growth of P.notoginseng is unknown.In this study,the effects of different soil moisture on the growth of P.notoginseng were studied.Methods:Four different water treatments(0.45 field capacity(FC),0.60 FC, 0.70 FC, and 0.85 FC) were set up in Shilin County,Yunnan Province,China.The water consumption and daily dynamic of water consumption were determined daily(from April 21 to October 18,2012),and the daily dynamic of water consumption under different weather conditions(sunny and rainy) was determined.The transpiration coefficient and water use efficiency were calculated through dry matter accumulation and total water consumption.Accumulation of saponins of roots of P.notoginseng were analyzed by HPLC after treated,and the soil moisture content suitable for the growth of P.notoginseng was estimated by regression fitting of the active ingredient accumulation and the soil moisture content.Results:The water consumption of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.89,3.68,3.37 and 2.73 kg/plant per day,respectively.The water consumption of P.notoginseng from June to August was greater than other months.The daily dynamic of water consumption on sunny days and sunny days after rain showed a "double peak" feature,and it showed a "single peak" feature on rainy days.The water uses efficiency(WUE) of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.51,3.32,4.59,3.39 gDW/kg H_(2)O,respectively.The increase of soil moistu re content would reduce the WUE of P.notoginse ng.With the increase of soil water content,the content of notoginsenoside R_(1) and ginsenoside Rg_(1) did not change significantly,while the content of ginsenoside Rb_(1) and Rd showed a decreasing trend.Conclusion:Soil moisture content significantly affected the water consumption of P.notoginseng,and when it was 56.4% of the maximum water holding capacity in the field,the sum of the four saponins of 100 strains of P.notoginseng was the highest.
